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The map label for this gene is lysS [H]

Identifier: 229917390

GI number: 229917390

Start: 1613309

End: 1614799

Strand: Reverse

Name: lysS [H]

Synonym: EAT1b_1665

Alternate gene names: 229917390

Gene position: 1614799-1613309 (Counterclockwise)

Preceding gene: 229917391

Following gene: 229917389

Centisome position: 53.83

GC content: 45.74

Gene sequence:

>1491_bases
GTGAGCCACGAGTTAGAAATGAATGATCAAATGCAGGTTCGTTTTGAGAAGATGACCGATATGCGTGAGCGCGGTCTCGA
CCCGTTCGGTCACCGCTTTGATCGTTCTGCACACGCAGGTGAATTACATGAGCAGTATGAATCAATTGAAAAAGAGGCGT
TGGCTGAACAAGATGTAGAAGTCACATTGGCTGGACGCATCATGACGAAACGTGGGAAAGGGAAGGTTTTCTTTGCCCAC
ATTCAAGATGTGACAGGTCAAATTCAAATTTACGTTCGTAAAAATGATGTCGGGGATGAGCCGTTTGACTTATTCAAGTC
TTCAGACCTCGGTGACATCGTCGGTGTAAAAGGGAAGCTCTTTAAAACGAACGTTGGTGAGCTTTCGATTCACGTGGAAG
AGTTTGAATTATTGACGAAGGCGCTTCGCCCGCTTCCGGACAAATACCATGGATTAAAAGACGTGGAACAGCGCTATCGC
CAACGCTATCTTGATTTGATTACAAACCAGGACTCGCGTCAGACATTCATTTTGCGTAGCCGCATCATTTCGGAGATTCG
CAACTTCTTAAATCAAAAAGGATATTTAGAAGTGGAAACACCGATGTTACACACGATCGCGGGTGGAGCAGCGGCGCGTC
CGTTCTTAACGCATCATAATGCGTTGGATATGGAACTTTATATGCGTATTGCCATCGAACTTCACTTGAAACGCTTGATC
GTTGGTGGACTTGAGCGCGTTTACGAAATCGGACGTGTATTCCGTAACGAAGGGATCTCTACACGTCACAACCCTGAATT
CACGATGATCGAATTGTATGAGGCCTATGCAGATTACAACGACATCATGGATTTGACGGAAGAATTGGTTGCGCACGTAG
CGAAAGAAGTACTCGGTACAACAGATGTGCAGTATGGTGAAGATACAATTCATCTTGGCATCGGTTGGAAACGTGCACAT
ATGGTTGACCTTGTTAAAGAAGAGACAGGTGTCGACTTCTGGCAACAAATGTCCGATGAAGAAGCACGTGCTCTCGCAAA
AGAACACGGTGTAGAAGTACAAGATCATATGACGTTTGGTCATATCGTGAATGAATTCTTTGAGCAGAAAGTAGAAGAAA
CGTTGCTTCAACCGACATTTGTCACAGGACACCCGGTTGAAGTATCACCATTGGCGAAGAAGAATGAAACAGATCCACGC
TTCACGGATCGTTTCGAGTTGTTCATTGTTCGTCGTGAACATGCGAATGCCTTTACGGAGTTGAATGACCCAATCGATCA
ACGTGAACGCTTTGAAGCACAGTTGCTTGAAAAAGAAGCAGGTAACGATGAGGCACACGAACTCGACAACGATTTCCTTG
AGGCACTCGAATATGGTATGCCGCCAACAGGCGGACTTGGAATCGGGATTGACCGCCTCGTCATGTTATTGACGAACGCG
CCTTCAATCCGTGACGTTCTTCTTTTCCCAACGATGCGTCATCAACAATAA

Upstream 100 bases:

>100_bases
CACCACAACGAATGAACGGCTTCGGCTGTGCTGTTGGCCATCGGGTCGACAGTTTTTTGTTAGAGAGAAACCCAATATGG
ATAGAGGAGTGAATGGAATA

Downstream 100 bases:

>100_bases
TCAATTTAGTGGCCGGATCATCCGTGAGTATGATCCGGCTTCTTTTTTTTTGTCAGCCTCGAAACGCATAGGTAAGCCGT
TAAACAGAAGGAATACTATA

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MSHELEMNDQMQVRFEKMTDMRERGLDPFGHRFDRSAHAGELHEQYESIEKEALAEQDVEVTLAGRIMTKRGKGKVFFAH
IQDVTGQIQIYVRKNDVGDEPFDLFKSSDLGDIVGVKGKLFKTNVGELSIHVEEFELLTKALRPLPDKYHGLKDVEQRYR
QRYLDLITNQDSRQTFILRSRIISEIRNFLNQKGYLEVETPMLHTIAGGAAARPFLTHHNALDMELYMRIAIELHLKRLI
VGGLERVYEIGRVFRNEGISTRHNPEFTMIELYEAYADYNDIMDLTEELVAHVAKEVLGTTDVQYGEDTIHLGIGWKRAH
MVDLVKEETGVDFWQQMSDEEARALAKEHGVEVQDHMTFGHIVNEFFEQKVEETLLQPTFVTGHPVEVSPLAKKNETDPR
FTDRFELFIVRREHANAFTELNDPIDQRERFEAQLLEKEAGNDEAHELDNDFLEALEYGMPPTGGLGIGIDRLVMLLTNA
PSIRDVLLFPTMRHQQ

Sequences:

>Translated_496_residues
MSHELEMNDQMQVRFEKMTDMRERGLDPFGHRFDRSAHAGELHEQYESIEKEALAEQDVEVTLAGRIMTKRGKGKVFFAH
IQDVTGQIQIYVRKNDVGDEPFDLFKSSDLGDIVGVKGKLFKTNVGELSIHVEEFELLTKALRPLPDKYHGLKDVEQRYR
QRYLDLITNQDSRQTFILRSRIISEIRNFLNQKGYLEVETPMLHTIAGGAAARPFLTHHNALDMELYMRIAIELHLKRLI
VGGLERVYEIGRVFRNEGISTRHNPEFTMIELYEAYADYNDIMDLTEELVAHVAKEVLGTTDVQYGEDTIHLGIGWKRAH
MVDLVKEETGVDFWQQMSDEEARALAKEHGVEVQDHMTFGHIVNEFFEQKVEETLLQPTFVTGHPVEVSPLAKKNETDPR
FTDRFELFIVRREHANAFTELNDPIDQRERFEAQLLEKEAGNDEAHELDNDFLEALEYGMPPTGGLGIGIDRLVMLLTNA
PSIRDVLLFPTMRHQQ
>Mature_495_residues
SHELEMNDQMQVRFEKMTDMRERGLDPFGHRFDRSAHAGELHEQYESIEKEALAEQDVEVTLAGRIMTKRGKGKVFFAHI
QDVTGQIQIYVRKNDVGDEPFDLFKSSDLGDIVGVKGKLFKTNVGELSIHVEEFELLTKALRPLPDKYHGLKDVEQRYRQ
RYLDLITNQDSRQTFILRSRIISEIRNFLNQKGYLEVETPMLHTIAGGAAARPFLTHHNALDMELYMRIAIELHLKRLIV
GGLERVYEIGRVFRNEGISTRHNPEFTMIELYEAYADYNDIMDLTEELVAHVAKEVLGTTDVQYGEDTIHLGIGWKRAHM
VDLVKEETGVDFWQQMSDEEARALAKEHGVEVQDHMTFGHIVNEFFEQKVEETLLQPTFVTGHPVEVSPLAKKNETDPRF
TDRFELFIVRREHANAFTELNDPIDQRERFEAQLLEKEAGNDEAHELDNDFLEALEYGMPPTGGLGIGIDRLVMLLTNAP
SIRDVLLFPTMRHQQ

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=503, Percent_Identity=42.1471172962227, Blast_Score=390, Evalue=1e-108,
Organism=Homo sapiens, GI194272210, Length=503, Percent_Identity=42.1471172962227, Blast_Score=389, Evalue=1e-108,
Organism=Homo sapiens, GI45439306, Length=480, Percent_Identity=22.9166666666667, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI40789249, Length=320, Percent_Identity=27.1875, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI226491502, Length=499, Percent_Identity=22.8456913827655, Blast_Score=83, Evalue=7e-16,
Organism=Escherichia coli, GI1790571, Length=494, Percent_Identity=53.2388663967611, Blast_Score=536, Evalue=1e-153,
Organism=Escherichia coli, GI1789256, Length=491, Percent_Identity=53.3604887983707, Blast_Score=533, Evalue=1e-152,
Organism=Escherichia coli, GI87082379, Length=321, Percent_Identity=32.398753894081, Blast_Score=141, Evalue=7e-35,
Organism=Escherichia coli, GI1788173, Length=311, Percent_Identity=25.4019292604502, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17535925, Length=489, Percent_Identity=45.8077709611452, Blast_Score=409, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17535927, Length=489, Percent_Identity=45.8077709611452, Blast_Score=408, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI71994340, Length=489, Percent_Identity=45.8077709611452, Blast_Score=408, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17551876, Length=467, Percent_Identity=23.1263383297645, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI32566633, Length=181, Percent_Identity=29.2817679558011, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320242, Length=491, Percent_Identity=45.213849287169, Blast_Score=402, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6324256, Length=480, Percent_Identity=32.7083333333333, Blast_Score=209, Evalue=1e-54,
Organism=Saccharomyces cerevisiae, GI6323011, Length=477, Percent_Identity=24.3186582809224, Blast_Score=100, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6321807, Length=445, Percent_Identity=25.1685393258427, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6325153, Length=245, Percent_Identity=26.9387755102041, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24640851, Length=507, Percent_Identity=43.3925049309665, Blast_Score=412, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24640849, Length=507, Percent_Identity=43.3925049309665, Blast_Score=412, Evalue=1e-115,
Organism=Drosophila melanogaster, GI17136276, Length=469, Percent_Identity=23.0277185501066, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24584738, Length=228, Percent_Identity=26.7543859649123, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 57234; Mature: 57102

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHELEMNDQMQVRFEKMTDMRERGLDPFGHRFDRSAHAGELHEQYESIEKEALAEQDVE
CCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCE
VTLAGRIMTKRGKGKVFFAHIQDVTGQIQIYVRKNDVGDEPFDLFKSSDLGDIVGVKGKL
EEEECEEEEECCCCCEEEEEEECCCCEEEEEEEECCCCCCHHHHHCCCCCCCHHCCCCEE
FKTNVGELSIHVEEFELLTKALRPLPDKYHGLKDVEQRYRQRYLDLITNQDSRQTFILRS
EECCCCCEEEEHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
RIISEIRNFLNQKGYLEVETPMLHTIAGGAAARPFLTHHNALDMELYMRIAIELHLKRLI
HHHHHHHHHHCCCCEEEECCCHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHH
VGGLERVYEIGRVFRNEGISTRHNPEFTMIELYEAYADYNDIMDLTEELVAHVAKEVLGT
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCC
TDVQYGEDTIHLGIGWKRAHMVDLVKEETGVDFWQQMSDEEARALAKEHGVEVQDHMTFG
CCCCCCCCEEEEECCCHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHCCCHHHHHHHH
HIVNEFFEQKVEETLLQPTFVTGHPVEVSPLAKKNETDPRFTDRFELFIVRREHANAFTE
HHHHHHHHHHHHHHHCCCCEECCCCCEECCCCCCCCCCCCCCCCEEEEEEEEHHCCHHHH
LNDPIDQRERFEAQLLEKEAGNDEAHELDNDFLEALEYGMPPTGGLGIGIDRLVMLLTNA
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCC
PSIRDVLLFPTMRHQQ
CCHHHEEECCCCCCCC
>Mature Secondary Structure 
SHELEMNDQMQVRFEKMTDMRERGLDPFGHRFDRSAHAGELHEQYESIEKEALAEQDVE
CCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCE
VTLAGRIMTKRGKGKVFFAHIQDVTGQIQIYVRKNDVGDEPFDLFKSSDLGDIVGVKGKL
EEEECEEEEECCCCCEEEEEEECCCCEEEEEEEECCCCCCHHHHHCCCCCCCHHCCCCEE
FKTNVGELSIHVEEFELLTKALRPLPDKYHGLKDVEQRYRQRYLDLITNQDSRQTFILRS
EECCCCCEEEEHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
RIISEIRNFLNQKGYLEVETPMLHTIAGGAAARPFLTHHNALDMELYMRIAIELHLKRLI
HHHHHHHHHHCCCCEEEECCCHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHH
VGGLERVYEIGRVFRNEGISTRHNPEFTMIELYEAYADYNDIMDLTEELVAHVAKEVLGT
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCC
TDVQYGEDTIHLGIGWKRAHMVDLVKEETGVDFWQQMSDEEARALAKEHGVEVQDHMTFG
CCCCCCCCEEEEECCCHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHCCCHHHHHHHH
HIVNEFFEQKVEETLLQPTFVTGHPVEVSPLAKKNETDPRFTDRFELFIVRREHANAFTE
HHHHHHHHHHHHHHHCCCCEECCCCCEECCCCCCCCCCCCCCCCEEEEEEEEHHCCHHHH
LNDPIDQRERFEAQLLEKEAGNDEAHELDNDFLEALEYGMPPTGGLGIGIDRLVMLLTNA
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCC
PSIRDVLLFPTMRHQQ
CCHHHEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA