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The map label for this gene is radA [H]

Identifier: 229917385

GI number: 229917385

Start: 1602157

End: 1603533

Strand: Reverse

Name: radA [H]

Synonym: EAT1b_1660

Alternate gene names: 229917385

Gene position: 1603533-1602157 (Counterclockwise)

Preceding gene: 229917386

Following gene: 229917384

Centisome position: 53.45

GC content: 52.87

Gene sequence:

>1377_bases
ATGGCCAAAATTAAAACGAAATACGTCTGCCAATCGTGCGGATATGAATCAGCCAAGTGGATGGGGCGTTGTCCGGGTTG
TAATGAATGGAATACGCTCGTCGAAGAGTTTGTAGAAGAGAAGAAAGCCAAACGTGGAGCGGCCTTTGTGCATAGTTCAT
CGCGACAATTGAAACCGGAACGCTTAGCAAACGTCGTCTCACAAGAAGAGACGCGTGTGCATACGAATAGCCGTGAATTT
GACCGCGTGCTCGGTGGAGGGATTGTCCCAGGTTCACTCGTCTTAGTCGGAGGGGACCCAGGGATTGGGAAATCGACGAT
ACTGCTACAGGTCAGCGCTCACTTAGCCCATAGTGGTAAAAAGGTGCTGTACATCTCGGGAGAGGAATCGCTCAAACAGA
CGAAACTGCGTGCGGAACGGCTTGGCTTACCGACGGATGACTTGTTCGTACTCGCTGAGACGGACATGTTGATGATCGAA
CGGGTTATTGATGAGGAGCAACCTGGATTTGTCATCATTGACTCGATTCAAACGGTATATATGGATGAGATTCAATCAGC
GCCAGGAAGCGTGTCACAAGTTCGAGAATGTACGGCCGCCCTCATGAAAATTGCGAAAACGCGAGGGATTGCCGTCTTCA
TCGTCGGTCACGTGACGAAGCAAGGGTCGATTGCCGGCCCGCGCTTACTTGAACATATGGTCGATGCGGTACTTTATTTT
GAAGGGGAGCGTCATCATACGTTCCGTATTTTACGGGCGGTAAAAAACCGTTTTGGGTCGACGAACGAGATTGGCATTTT
CGAGATGAAAGAGAGCGGACTAGAGGAAGTGCTCAATCCGTCCGAAATATTCTTGGAGGAACGCACTGCGGGCGTATCGG
GTTCGACCGTCGTTGCCTCGATGGAAGGTACGCGGACGGTACTCGTGGAACTCCAAGCACTCATCTCTCCGACATCGTTC
GGGAACCCGAGACGTATGGCCACGGGCATCGATCAAAACCGAGTCGCGCTGCTCATGGCCGTGCTCGAAAAACGTTCCGG
CCTCTTGCTTCAGACGCAGGATGCGTATTTGAAAGCAGCCGGTGGCGTCAAACTGGACGAACCGGCGATCGACCTTGCGA
TTTGTATCTCGATTGCCTCGAGTTTCCGGGATCGTCCGACCCGTCCGACGGATGTCTGCATCGGTGAGGTCGGATTGACC
GGAGAAGTGCGAAGGGTTTCCCGAATCGAGCAACGTGTCGCTGAGGCGGCAAAACTAGGTTTTACACGTGCCATCATCCC
GAAAAATAACATGGGGGGATGGACCGCTCCGGCAGGGATTGAAGTCGTCGGGGTCGGAACAGTGGACGAAGCACTCCAGT
TGGCGATTCCGTTCTAA

Upstream 100 bases:

>100_bases
AAGATGGAGAGTTCGTCGTTCGTAACGAAGGTGTAGTTCAGTAAAACATTCAGCTCCCTCGTCGTTTAGAGGGAGCTTTT
TTTAGACAGGAAGGGAACTT

Downstream 100 bases:

>100_bases
CGGGTCGCCACTGGCAGTTTTTAACGCTTTGTAACGAACGTGATTGAAAAAATCGTGTTACAATGAAAGTGTTATTTTTT
GAAATTTTATTTCATATAGA

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MAKIKTKYVCQSCGYESAKWMGRCPGCNEWNTLVEEFVEEKKAKRGAAFVHSSSRQLKPERLANVVSQEETRVHTNSREF
DRVLGGGIVPGSLVLVGGDPGIGKSTILLQVSAHLAHSGKKVLYISGEESLKQTKLRAERLGLPTDDLFVLAETDMLMIE
RVIDEEQPGFVIIDSIQTVYMDEIQSAPGSVSQVRECTAALMKIAKTRGIAVFIVGHVTKQGSIAGPRLLEHMVDAVLYF
EGERHHTFRILRAVKNRFGSTNEIGIFEMKESGLEEVLNPSEIFLEERTAGVSGSTVVASMEGTRTVLVELQALISPTSF
GNPRRMATGIDQNRVALLMAVLEKRSGLLLQTQDAYLKAAGGVKLDEPAIDLAICISIASSFRDRPTRPTDVCIGEVGLT
GEVRRVSRIEQRVAEAAKLGFTRAIIPKNNMGGWTAPAGIEVVGVGTVDEALQLAIPF

Sequences:

>Translated_458_residues
MAKIKTKYVCQSCGYESAKWMGRCPGCNEWNTLVEEFVEEKKAKRGAAFVHSSSRQLKPERLANVVSQEETRVHTNSREF
DRVLGGGIVPGSLVLVGGDPGIGKSTILLQVSAHLAHSGKKVLYISGEESLKQTKLRAERLGLPTDDLFVLAETDMLMIE
RVIDEEQPGFVIIDSIQTVYMDEIQSAPGSVSQVRECTAALMKIAKTRGIAVFIVGHVTKQGSIAGPRLLEHMVDAVLYF
EGERHHTFRILRAVKNRFGSTNEIGIFEMKESGLEEVLNPSEIFLEERTAGVSGSTVVASMEGTRTVLVELQALISPTSF
GNPRRMATGIDQNRVALLMAVLEKRSGLLLQTQDAYLKAAGGVKLDEPAIDLAICISIASSFRDRPTRPTDVCIGEVGLT
GEVRRVSRIEQRVAEAAKLGFTRAIIPKNNMGGWTAPAGIEVVGVGTVDEALQLAIPF
>Mature_457_residues
AKIKTKYVCQSCGYESAKWMGRCPGCNEWNTLVEEFVEEKKAKRGAAFVHSSSRQLKPERLANVVSQEETRVHTNSREFD
RVLGGGIVPGSLVLVGGDPGIGKSTILLQVSAHLAHSGKKVLYISGEESLKQTKLRAERLGLPTDDLFVLAETDMLMIER
VIDEEQPGFVIIDSIQTVYMDEIQSAPGSVSQVRECTAALMKIAKTRGIAVFIVGHVTKQGSIAGPRLLEHMVDAVLYFE
GERHHTFRILRAVKNRFGSTNEIGIFEMKESGLEEVLNPSEIFLEERTAGVSGSTVVASMEGTRTVLVELQALISPTSFG
NPRRMATGIDQNRVALLMAVLEKRSGLLLQTQDAYLKAAGGVKLDEPAIDLAICISIASSFRDRPTRPTDVCIGEVGLTG
EVRRVSRIEQRVAEAAKLGFTRAIIPKNNMGGWTAPAGIEVVGVGTVDEALQLAIPF

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=461, Percent_Identity=47.5054229934924, Blast_Score=430, Evalue=1e-122,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 49846; Mature: 49715

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIKTKYVCQSCGYESAKWMGRCPGCNEWNTLVEEFVEEKKAKRGAAFVHSSSRQLKPE
CCCCCHHHHHHHCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHCCHHEEECCCCCCCHH
RLANVVSQEETRVHTNSREFDRVLGGGIVPGSLVLVGGDPGIGKSTILLQVSAHLAHSGK
HHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCEEEECCCCCCCCCEEEEEEEHHHHCCCC
KVLYISGEESLKQTKLRAERLGLPTDDLFVLAETDMLMIERVIDEEQPGFVIIDSIQTVY
EEEEECCHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHCCCCCCEEEECCHHHHH
MDEIQSAPGSVSQVRECTAALMKIAKTRGIAVFIVGHVTKQGSIAGPRLLEHMVDAVLYF
HHHHHCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHC
EGERHHTFRILRAVKNRFGSTNEIGIFEMKESGLEEVLNPSEIFLEERTAGVSGSTVVAS
CCCCHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHCCHHHHHHHHHCCCCCCCEEEEE
MEGTRTVLVELQALISPTSFGNPRRMATGIDQNRVALLMAVLEKRSGLLLQTQDAYLKAA
CCCCHHHHHHHHHHHCCCCCCCCHHHHCCCCCHHHHHHHHHHHHCCCCEEEECHHHHHHC
GGVKLDEPAIDLAICISIASSFRDRPTRPTDVCIGEVGLTGEVRRVSRIEQRVAEAAKLG
CCCEECCCHHHHHHHHHHHHHHCCCCCCCHHEEEECCCCCHHHHHHHHHHHHHHHHHHHC
FTRAIIPKNNMGGWTAPAGIEVVGVGTVDEALQLAIPF
CEEEECCCCCCCCCCCCCCEEEEECCCHHHHHHHCCCC
>Mature Secondary Structure 
AKIKTKYVCQSCGYESAKWMGRCPGCNEWNTLVEEFVEEKKAKRGAAFVHSSSRQLKPE
CCCCHHHHHHHCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHCCHHEEECCCCCCCHH
RLANVVSQEETRVHTNSREFDRVLGGGIVPGSLVLVGGDPGIGKSTILLQVSAHLAHSGK
HHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCEEEECCCCCCCCCEEEEEEEHHHHCCCC
KVLYISGEESLKQTKLRAERLGLPTDDLFVLAETDMLMIERVIDEEQPGFVIIDSIQTVY
EEEEECCHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHCCCCCCEEEECCHHHHH
MDEIQSAPGSVSQVRECTAALMKIAKTRGIAVFIVGHVTKQGSIAGPRLLEHMVDAVLYF
HHHHHCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHC
EGERHHTFRILRAVKNRFGSTNEIGIFEMKESGLEEVLNPSEIFLEERTAGVSGSTVVAS
CCCCHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHCCHHHHHHHHHCCCCCCCEEEEE
MEGTRTVLVELQALISPTSFGNPRRMATGIDQNRVALLMAVLEKRSGLLLQTQDAYLKAA
CCCCHHHHHHHHHHHCCCCCCCCHHHHCCCCCHHHHHHHHHHHHCCCCEEEECHHHHHHC
GGVKLDEPAIDLAICISIASSFRDRPTRPTDVCIGEVGLTGEVRRVSRIEQRVAEAAKLG
CCCEECCCHHHHHHHHHHHHHHCCCCCCCHHEEEECCCCCHHHHHHHHHHHHHHHHHHHC
FTRAIIPKNNMGGWTAPAGIEVVGVGTVDEALQLAIPF
CEEEECCCCCCCCCCCCCCEEEEECCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 8016066 [H]