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The map label for this gene is ispD [H]

Identifier: 229917383

GI number: 229917383

Start: 1600242

End: 1600922

Strand: Reverse

Name: ispD [H]

Synonym: EAT1b_1658

Alternate gene names: 229917383

Gene position: 1600922-1600242 (Counterclockwise)

Preceding gene: 229917384

Following gene: 229917382

Centisome position: 53.37

GC content: 51.98

Gene sequence:

>681_bases
ATGGTAGCTTATACAGTTGTCATCCCGGCTGCCGGGAGAGGGAAGCGGATGGGTGCGGATGCGAATAAGCTCATGCTGAC
GCTCCGTGACAAACCGATTATTGCTTGGACGTTACAGGCGTTCGATACCGATCCGTGGTGCGAACGAATGGTGTTAGCCA
TCCGGCCAGAAGAACGTGACTGGTTCGAGGCGATGACGTCACAGATGAAGACGCCGGTTACATACGTAGCAGGAGGGGAA
GAACGACAACAGAGTGTCCATGCAGGACTGAAGGTTGTTGCACCCGATACGATTGTGATGATTCATGACGGTGCGCGTCC
GTTTGTAAAACGTGAACAGTTGCATCAAGTGGCTGAAGCGGCACTGCAAGGTGGTGCCATCTTGGCGGTACCCGTTAAAG
ATACGGTCAAACAAGTTCAACGTCATCACATCGAACGCACCGTTCCCCGTGAAAACTTATGGTTGGCACAAACGCCGCAA
GCCTTTCAGGCAAAAGCGATTTTGGCGGCACATGAACGAGCGGTCGAACAACAGGTGATGGGAACGGATGATGCCAGTTT
GTTTGAGTGGCTCGGCGAATCGGTACAAGTGGTCCTTGGGGACTATCACAACATCAAAATGACGACGCCCGAAGATTTAT
TGTTCGGCGACGCGATTTTAGCGAAGGAGGAATTACAATGA

Upstream 100 bases:

>100_bases
TTACAAACGTCTGCAGGCCGTATGATTTTTGCCCGTCCAGAATAACGGATCAAAAAGAGACGCGAGAGCGCTCTCTTTTT
TCACGAAGAGAGGTATTAAC

Downstream 100 bases:

>100_bases
GAATTGGTCAAGGCTTTGATGTACACGCATTTGCAGAAGGACGTCCGTTAATCTTAGGAGGGATTGAGATTCCACATGAG
CGCGGACTGCTCGGTCATTC

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT [H]

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MVAYTVVIPAAGRGKRMGADANKLMLTLRDKPIIAWTLQAFDTDPWCERMVLAIRPEERDWFEAMTSQMKTPVTYVAGGE
ERQQSVHAGLKVVAPDTIVMIHDGARPFVKREQLHQVAEAALQGGAILAVPVKDTVKQVQRHHIERTVPRENLWLAQTPQ
AFQAKAILAAHERAVEQQVMGTDDASLFEWLGESVQVVLGDYHNIKMTTPEDLLFGDAILAKEELQ

Sequences:

>Translated_226_residues
MVAYTVVIPAAGRGKRMGADANKLMLTLRDKPIIAWTLQAFDTDPWCERMVLAIRPEERDWFEAMTSQMKTPVTYVAGGE
ERQQSVHAGLKVVAPDTIVMIHDGARPFVKREQLHQVAEAALQGGAILAVPVKDTVKQVQRHHIERTVPRENLWLAQTPQ
AFQAKAILAAHERAVEQQVMGTDDASLFEWLGESVQVVLGDYHNIKMTTPEDLLFGDAILAKEELQ
>Mature_226_residues
MVAYTVVIPAAGRGKRMGADANKLMLTLRDKPIIAWTLQAFDTDPWCERMVLAIRPEERDWFEAMTSQMKTPVTYVAGGE
ERQQSVHAGLKVVAPDTIVMIHDGARPFVKREQLHQVAEAALQGGAILAVPVKDTVKQVQRHHIERTVPRENLWLAQTPQ
AFQAKAILAAHERAVEQQVMGTDDASLFEWLGESVQVVLGDYHNIKMTTPEDLLFGDAILAKEELQ

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP) [H]

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family [H]

Homologues:

Organism=Homo sapiens, GI157412259, Length=232, Percent_Identity=27.5862068965517, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI157671913, Length=134, Percent_Identity=32.089552238806, Blast_Score=73, Evalue=2e-13,
Organism=Escherichia coli, GI1789104, Length=221, Percent_Identity=36.1990950226244, Blast_Score=124, Evalue=5e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR018294 [H]

Pfam domain/function: PF01128 IspD [H]

EC number: =2.7.7.60 [H]

Molecular weight: Translated: 25229; Mature: 25229

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAYTVVIPAAGRGKRMGADANKLMLTLRDKPIIAWTLQAFDTDPWCERMVLAIRPEERD
CEEEEEEEECCCCCCCCCCCCCCEEEEECCCCEEEEEEEECCCCHHHHHEEEEECCCCHH
WFEAMTSQMKTPVTYVAGGEERQQSVHAGLKVVAPDTIVMIHDGARPFVKREQLHQVAEA
HHHHHHHHHCCCEEEEECCHHHHHHHHCCEEEECCCEEEEEECCCCCHHHHHHHHHHHHH
ALQGGAILAVPVKDTVKQVQRHHIERTVPRENLWLAQTPQAFQAKAILAAHERAVEQQVM
HHCCCCEEEECCHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHC
GTDDASLFEWLGESVQVVLGDYHNIKMTTPEDLLFGDAILAKEELQ
CCCCHHHHHHHCHHHEEEECCCCEEEECCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVAYTVVIPAAGRGKRMGADANKLMLTLRDKPIIAWTLQAFDTDPWCERMVLAIRPEERD
CEEEEEEEECCCCCCCCCCCCCCEEEEECCCCEEEEEEEECCCCHHHHHEEEEECCCCHH
WFEAMTSQMKTPVTYVAGGEERQQSVHAGLKVVAPDTIVMIHDGARPFVKREQLHQVAEA
HHHHHHHHHCCCEEEEECCHHHHHHHHCCEEEECCCEEEEEECCCCCHHHHHHHHHHHHH
ALQGGAILAVPVKDTVKQVQRHHIERTVPRENLWLAQTPQAFQAKAILAAHERAVEQQVM
HHCCCCEEEECCHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHC
GTDDASLFEWLGESVQVVLGDYHNIKMTTPEDLLFGDAILAKEELQ
CCCCHHHHHHHCHHHEEEECCCCEEEECCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA