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The map label for this gene is truA

Identifier: 229917326

GI number: 229917326

Start: 1557889

End: 1558629

Strand: Reverse

Name: truA

Synonym: EAT1b_1601

Alternate gene names: 229917326

Gene position: 1558629-1557889 (Counterclockwise)

Preceding gene: 229917327

Following gene: 229917325

Centisome position: 51.96

GC content: 51.28

Gene sequence:

>741_bases
ATGAGACGAATTAAACTGATTGTCGCCTATGACGGGACACATTATGCGGGATATCAGGTACAACCGAATGGCAATACCAT
TCAAGCGGAGATTGAATCCGTCTTGGCACGTATGCATCGTCATCCGGTGAAAATCATCGCCTCAGGTCGTACAGATGCTC
GCGTCCATGCGTTAGGGCAGGTGATTCACTTTGATACGACGTTAGAGATGCCGGCTGAGCGCTTCGTCAAAGCGTTAAAC
GCCATGCTACCAGACGATATCCTCGTTAAACAGGCGGAAGAAATGGATGATACGTTTCATGCACGTTACGGTGCGAAGCG
AAAAGAGTATCGCTATCATATCCGTCAGACCGAGGATCCGTTTCGTCGGCATTATGCTGCGACCGTGACGTATAAGCTGA
GCCTTGAGGCGATGCGTCAAGCGATGGAGCGGTTGATTGGGACTCACGACTTTACGTCGTTCTCTGTGACAAAGGCAGAA
GTCGAAGATCGGGTCCGCACCATCTACGAAGCAGAAGTGCTCGAGATGGGGGACGAGATCATATTCCGGTTCGTTGGCTC
GGGTTTTCTATACAACCAAGTCCGCATCATGGTAGGGACGGTCATCGAGGTGGGGAGAGGGAAGTATCAGCCCGCAGATA
TCACACAAATGCTGGAGGCGAAGGACCGCCGCGTGGCGGGGATTACGGCGCCCCCACACGGTTTGTATTTGTGGAATGTG
AATTATGAAAAAGTAGATTGA

Upstream 100 bases:

>100_bases
GGACGAGGTTGCGTGAGATGGAGTGGCAAATGCGAGACACAGCCGTATTGCTCATCTTTGTCGCGTTGACACTCATTTTG
TTTGGATTGAGAGGGATTGG

Downstream 100 bases:

>100_bases
CATTCCCCTTATAAAATCGTATTATATTCATTGGCATTTCATTTTCAAATTTAGAGATGAACCACAATCTTAGCCCCGTA
AACTAGGATTATGATAAAGC

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MRRIKLIVAYDGTHYAGYQVQPNGNTIQAEIESVLARMHRHPVKIIASGRTDARVHALGQVIHFDTTLEMPAERFVKALN
AMLPDDILVKQAEEMDDTFHARYGAKRKEYRYHIRQTEDPFRRHYAATVTYKLSLEAMRQAMERLIGTHDFTSFSVTKAE
VEDRVRTIYEAEVLEMGDEIIFRFVGSGFLYNQVRIMVGTVIEVGRGKYQPADITQMLEAKDRRVAGITAPPHGLYLWNV
NYEKVD

Sequences:

>Translated_246_residues
MRRIKLIVAYDGTHYAGYQVQPNGNTIQAEIESVLARMHRHPVKIIASGRTDARVHALGQVIHFDTTLEMPAERFVKALN
AMLPDDILVKQAEEMDDTFHARYGAKRKEYRYHIRQTEDPFRRHYAATVTYKLSLEAMRQAMERLIGTHDFTSFSVTKAE
VEDRVRTIYEAEVLEMGDEIIFRFVGSGFLYNQVRIMVGTVIEVGRGKYQPADITQMLEAKDRRVAGITAPPHGLYLWNV
NYEKVD
>Mature_246_residues
MRRIKLIVAYDGTHYAGYQVQPNGNTIQAEIESVLARMHRHPVKIIASGRTDARVHALGQVIHFDTTLEMPAERFVKALN
AMLPDDILVKQAEEMDDTFHARYGAKRKEYRYHIRQTEDPFRRHYAATVTYKLSLEAMRQAMERLIGTHDFTSFSVTKAE
VEDRVRTIYEAEVLEMGDEIIFRFVGSGFLYNQVRIMVGTVIEVGRGKYQPADITQMLEAKDRRVAGITAPPHGLYLWNV
NYEKVD

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=245, Percent_Identity=33.0612244897959, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI199559785, Length=286, Percent_Identity=26.5734265734266, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI70166645, Length=271, Percent_Identity=26.5682656826568, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI70166634, Length=269, Percent_Identity=26.7657992565056, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI70166599, Length=269, Percent_Identity=26.7657992565056, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1788657, Length=242, Percent_Identity=34.7107438016529, Blast_Score=145, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI71985046, Length=291, Percent_Identity=27.8350515463918, Blast_Score=87, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6321112, Length=272, Percent_Identity=24.6323529411765, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_EXISA (C4KZL4)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002885972.1
- GeneID:   7869930
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_1601
- OMA:   KGVHANN
- ProtClustDB:   PRK00021
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 28321; Mature: 28321

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: NA

Important sites: ACT_SITE 52-52

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRIKLIVAYDGTHYAGYQVQPNGNTIQAEIESVLARMHRHPVKIIASGRTDARVHALGQ
CCEEEEEEEECCCEECCEEECCCCCEEHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHH
VIHFDTTLEMPAERFVKALNAMLPDDILVKQAEEMDDTFHARYGAKRKEYRYHIRQTEDP
HHEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCH
FRRHYAATVTYKLSLEAMRQAMERLIGTHDFTSFSVTKAEVEDRVRTIYEAEVLEMGDEI
HHHHHEEEEEEEEEHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHH
IFRFVGSGFLYNQVRIMVGTVIEVGRGKYQPADITQMLEAKDRRVAGITAPPHGLYLWNV
HHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEEEECCCCCCEEEEEC
NYEKVD
CCEECC
>Mature Secondary Structure
MRRIKLIVAYDGTHYAGYQVQPNGNTIQAEIESVLARMHRHPVKIIASGRTDARVHALGQ
CCEEEEEEEECCCEECCEEECCCCCEEHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHH
VIHFDTTLEMPAERFVKALNAMLPDDILVKQAEEMDDTFHARYGAKRKEYRYHIRQTEDP
HHEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCH
FRRHYAATVTYKLSLEAMRQAMERLIGTHDFTSFSVTKAEVEDRVRTIYEAEVLEMGDEI
HHHHHEEEEEEEEEHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHH
IFRFVGSGFLYNQVRIMVGTVIEVGRGKYQPADITQMLEAKDRRVAGITAPPHGLYLWNV
HHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEEEECCCCCCEEEEEC
NYEKVD
CCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA