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The map label for this gene is trpA [H]

Identifier: 229917175

GI number: 229917175

Start: 1415181

End: 1415948

Strand: Reverse

Name: trpA [H]

Synonym: EAT1b_1449

Alternate gene names: 229917175

Gene position: 1415948-1415181 (Counterclockwise)

Preceding gene: 229917176

Following gene: 229917173

Centisome position: 47.2

GC content: 52.73

Gene sequence:

>768_bases
ATGAAACTAGAGACAGCGATTCGACAGCGAGAAGGAGCGGCGTTCGTTCCGTATATCATGGCCGGTGACGGCGGGATGGA
CCGGCTCATCCCGACCATCGTGGAACTAGAGCGGATGGGTGCGACGGCGATTGAACTCGGTATCCCTTTCTCCGATCCGG
TGGCAGATGGCCCAATCATTGAGGCGGCAGGAATGCGCGCGCTTGAAGCAGGTGTGACGCTTGAAATGGTATTGAACCAT
TTAATAGAAGGAAATGATCAATTTAACGTTCCAATCGTCTTGATGACCTACTTGAATCCAGTGTTTCGGTTCGGGGTCCA
ACCGTTCTTTGATCGGGCGAACGAATGTGGGGTGAGCGGGGTCATTATTCCTGACCTTCCGTTTGAGGAGAGTCTCCAGT
TATTCGCAGATGTTAATCGGCATGACGTCGCCGTCATTCCGCTCGTCACGTTGACGAGCAGTGAGGCGCGGACAACGTCC
ATCTTAGAAAAAGCAGAAGGATTCGTGTATGCGGTCACGCTTAAAGGGACGACCGGGAAAGCAGATGTGTTCCCGGACGA
GTTACTCGCCTACTTGACTCGTTTGACAGAGCAGTCACGTGTTCCCGTCTTGGCAGGATTCGGAGTCCATCGTCCGGAGC
AAGTGCAGACGTTAGGACAGGCGTGTGACGGTGTCGTCGTCGGGAGTTTCATTGTCGAGGCACTTCATGAAGGACGGACA
GAAGATGTTGAGACGTTGATTCAATCAGCAAAACACTTACACGCATAA

Upstream 100 bases:

>100_bases
CGCTCGCGAAGACGATGGGAGAAAACGATACACTGGTGATTTGTTTATCAGGGCGCGGAGACAAAGACGTCCATACAATC
CGAGAGAAGGTGGCGACACG

Downstream 100 bases:

>100_bases
GAAAACCGGCGCTTCAAATGAGGCGCCGGTTATTCGTTTACTCATAATTCCACTCGGTCTAGCCCATCTTCACCAAATGT
TTGGTCGAGTTGATCATGAC

Product: tryptophan synthase, subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MKLETAIRQREGAAFVPYIMAGDGGMDRLIPTIVELERMGATAIELGIPFSDPVADGPIIEAAGMRALEAGVTLEMVLNH
LIEGNDQFNVPIVLMTYLNPVFRFGVQPFFDRANECGVSGVIIPDLPFEESLQLFADVNRHDVAVIPLVTLTSSEARTTS
ILEKAEGFVYAVTLKGTTGKADVFPDELLAYLTRLTEQSRVPVLAGFGVHRPEQVQTLGQACDGVVVGSFIVEALHEGRT
EDVETLIQSAKHLHA

Sequences:

>Translated_255_residues
MKLETAIRQREGAAFVPYIMAGDGGMDRLIPTIVELERMGATAIELGIPFSDPVADGPIIEAAGMRALEAGVTLEMVLNH
LIEGNDQFNVPIVLMTYLNPVFRFGVQPFFDRANECGVSGVIIPDLPFEESLQLFADVNRHDVAVIPLVTLTSSEARTTS
ILEKAEGFVYAVTLKGTTGKADVFPDELLAYLTRLTEQSRVPVLAGFGVHRPEQVQTLGQACDGVVVGSFIVEALHEGRT
EDVETLIQSAKHLHA
>Mature_255_residues
MKLETAIRQREGAAFVPYIMAGDGGMDRLIPTIVELERMGATAIELGIPFSDPVADGPIIEAAGMRALEAGVTLEMVLNH
LIEGNDQFNVPIVLMTYLNPVFRFGVQPFFDRANECGVSGVIIPDLPFEESLQLFADVNRHDVAVIPLVTLTSSEARTTS
ILEKAEGFVYAVTLKGTTGKADVFPDELLAYLTRLTEQSRVPVLAGFGVHRPEQVQTLGQACDGVVVGSFIVEALHEGRT
EDVETLIQSAKHLHA

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate [H]

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family [H]

Homologues:

Organism=Escherichia coli, GI1787514, Length=211, Percent_Identity=35.0710900473934, Blast_Score=128, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6321412, Length=255, Percent_Identity=34.9019607843137, Blast_Score=147, Evalue=1e-36,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028 [H]

Pfam domain/function: PF00290 Trp_syntA [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 27630; Mature: 27630

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLETAIRQREGAAFVPYIMAGDGGMDRLIPTIVELERMGATAIELGIPFSDPVADGPII
CCCHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEE
EAAGMRALEAGVTLEMVLNHLIEGNDQFNVPIVLMTYLNPVFRFGVQPFFDRANECGVSG
HHHCCHHHHHCCCHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHCCCHHHHHHHHCCCCC
VIIPDLPFEESLQLFADVNRHDVAVIPLVTLTSSEARTTSILEKAEGFVYAVTLKGTTGK
EEECCCCHHHHHHHHHCCCCCCEEEEEEEEECCCCHHHHHHHHHCCCEEEEEEEECCCCC
ADVFPDELLAYLTRLTEQSRVPVLAGFGVHRPEQVQTLGQACDGVVVGSFIVEALHEGRT
CCCCHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCC
EDVETLIQSAKHLHA
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MKLETAIRQREGAAFVPYIMAGDGGMDRLIPTIVELERMGATAIELGIPFSDPVADGPII
CCCHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEE
EAAGMRALEAGVTLEMVLNHLIEGNDQFNVPIVLMTYLNPVFRFGVQPFFDRANECGVSG
HHHCCHHHHHCCCHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHCCCHHHHHHHHCCCCC
VIIPDLPFEESLQLFADVNRHDVAVIPLVTLTSSEARTTSILEKAEGFVYAVTLKGTTGK
EEECCCCHHHHHHHHHCCCCCCEEEEEEEEECCCCHHHHHHHHHCCCEEEEEEEECCCCC
ADVFPDELLAYLTRLTEQSRVPVLAGFGVHRPEQVQTLGQACDGVVVGSFIVEALHEGRT
CCCCHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCC
EDVETLIQSAKHLHA
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376 [H]