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The map label for this gene is amyA [C]

Identifier: 229917132

GI number: 229917132

Start: 1378197

End: 1379741

Strand: Reverse

Name: amyA [C]

Synonym: EAT1b_1406

Alternate gene names: 229917132

Gene position: 1379741-1378197 (Counterclockwise)

Preceding gene: 229917133

Following gene: 229917131

Centisome position: 45.99

GC content: 50.87

Gene sequence:

>1545_bases
ATGTTGAAGAAACGACAAGGGATTGCCGTCCTGGCCGGAGTGACATCGATTGCACTGCTTTCAGGGCAACCGGTCGCACA
GGCAGCGACCCCACAGAACGGTACGATGATGCAATATTTTGAATGGTACGTTCCAAACGATGGGCAACACTGGAACCGAC
TCTCGAATGATTCGCAGCATTTGAAAGACATCGGGATCTCCACGGTTTGGATTCCGCCAGCGTATAAAGGGACGTCTCAA
AATGATGTCGGATACGGGGCGTATGATTTGTATGATTTAGGAGAGTTCAATCAAAAAGGGACGACTCGGACGAAGTATGG
AACGAAAGCGCAGCTACAGTCGGCAATCTCGAACTTACGCGGGAAAGGGATTGGCGTATATGGGGATGTCGTCATGAACC
ATAAGGGCGGAGCGGATTATACCGAGTCCGTTCAAGCGGTCGAGGTCAATCCTTCTAACCGAAATCAAGAGACGTCTGGG
GAATATGCCATTTCCGCCTGGACGGGATTCAACTTTGCGGGCCGCAACAATACATACTCGCCGTTCAAGTGGCGTTGGTA
TCACTTTGACGGGACTGACTGGGATCAGTCACGGAGTTTGAGCCGTATCTACAAATTCAAGAGCACGGGTAAAGCATGGG
ACAGTGAAGTGTCCGGGGAGAACGGAAATTATGACTACTTGATGTACGCCGATGTCGATTTTGAGCATCCGGAAGTACGT
CAGGAGATGAAAAACTGGGGGAAATGGTACGCAGATTCACTCGGATTGGACGGCTTCCGACTTGATGCGGTGAAGCATAT
CAATCATTCATACTTAAAGGAATGGGTGACGAGTGTCCGTCAGGCAACCGGGAAAGAGATGTTCACTGTTGCGGAGTATT
GGAAGAATGACTTAGGGGCCATCAATGATTACTTGGCGAAGACGGGCTACACTCATTCCGTATTCGATGTACCACTCCAT
TACAATTTCCAAGCGGCAGGGAACGGCGGCGGTTACTATGACATGCGGAACATCCTAAAAGGCACGGTCGTCGAGCAGCA
TCCTACACTCGCTGTCACCATTGTCGACAACCATGACTCACAACCAGGGCAGTCACTGGAATCAACGGTCGCGAATTGGT
TCAAACCGCTCGCCTATGCGACCATCATGACACGCGGTCAAGGATATCCAGCACTCTTCTACGGAGATTATTATGGAACG
AAAGGGACGACGAACCGTGAAATCCCGAATATGTCAGCGTCGCTTCAGCCGATTTTGAAAGCGCGTAAAGATTTCGCCTA
CGGTACACAACATGACTACATCAATCACCAAGACGTCATCGGGTGGACTCGTGAAGGGGTGACGGACCGTACGAAGTCAG
GTCTTGCGACGATTTTGTCGGACGGACCAGGCGGGTCGAAATGGATGTATGTCGGGAAGCAGAACGCGGGAGAAGTATGG
AAAGACATGACCGGCAACAATGGTCGTCTCGTGACGATCAATGCGGACGGTTGGGGCGAGTTCTTCGTCAATGGCGGCTC
GGTTTCCATCTATACGCAACAATGA

Upstream 100 bases:

>100_bases
GTGAGAACTGTTTTTGGACATCGGTCTATGCAACAAAATCAAAATCCTTCACAATAATTAATGCAAGCGCTTTCATTAAT
TATTTGAGGAGGAATGAAGG

Downstream 100 bases:

>100_bases
ACTCAGGTAGACTTGTCTCTAGCAACAAGAGACAAGTCTTTTTTCTGAATTTTCACCACATAAGTTTTATACGTGATATA
ATGACCTAGTGTTAATTCTT

Product: cytoplasmic alpha-amylase

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase [H]

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MLKKRQGIAVLAGVTSIALLSGQPVAQAATPQNGTMMQYFEWYVPNDGQHWNRLSNDSQHLKDIGISTVWIPPAYKGTSQ
NDVGYGAYDLYDLGEFNQKGTTRTKYGTKAQLQSAISNLRGKGIGVYGDVVMNHKGGADYTESVQAVEVNPSNRNQETSG
EYAISAWTGFNFAGRNNTYSPFKWRWYHFDGTDWDQSRSLSRIYKFKSTGKAWDSEVSGENGNYDYLMYADVDFEHPEVR
QEMKNWGKWYADSLGLDGFRLDAVKHINHSYLKEWVTSVRQATGKEMFTVAEYWKNDLGAINDYLAKTGYTHSVFDVPLH
YNFQAAGNGGGYYDMRNILKGTVVEQHPTLAVTIVDNHDSQPGQSLESTVANWFKPLAYATIMTRGQGYPALFYGDYYGT
KGTTNREIPNMSASLQPILKARKDFAYGTQHDYINHQDVIGWTREGVTDRTKSGLATILSDGPGGSKWMYVGKQNAGEVW
KDMTGNNGRLVTINADGWGEFFVNGGSVSIYTQQ

Sequences:

>Translated_514_residues
MLKKRQGIAVLAGVTSIALLSGQPVAQAATPQNGTMMQYFEWYVPNDGQHWNRLSNDSQHLKDIGISTVWIPPAYKGTSQ
NDVGYGAYDLYDLGEFNQKGTTRTKYGTKAQLQSAISNLRGKGIGVYGDVVMNHKGGADYTESVQAVEVNPSNRNQETSG
EYAISAWTGFNFAGRNNTYSPFKWRWYHFDGTDWDQSRSLSRIYKFKSTGKAWDSEVSGENGNYDYLMYADVDFEHPEVR
QEMKNWGKWYADSLGLDGFRLDAVKHINHSYLKEWVTSVRQATGKEMFTVAEYWKNDLGAINDYLAKTGYTHSVFDVPLH
YNFQAAGNGGGYYDMRNILKGTVVEQHPTLAVTIVDNHDSQPGQSLESTVANWFKPLAYATIMTRGQGYPALFYGDYYGT
KGTTNREIPNMSASLQPILKARKDFAYGTQHDYINHQDVIGWTREGVTDRTKSGLATILSDGPGGSKWMYVGKQNAGEVW
KDMTGNNGRLVTINADGWGEFFVNGGSVSIYTQQ
>Mature_514_residues
MLKKRQGIAVLAGVTSIALLSGQPVAQAATPQNGTMMQYFEWYVPNDGQHWNRLSNDSQHLKDIGISTVWIPPAYKGTSQ
NDVGYGAYDLYDLGEFNQKGTTRTKYGTKAQLQSAISNLRGKGIGVYGDVVMNHKGGADYTESVQAVEVNPSNRNQETSG
EYAISAWTGFNFAGRNNTYSPFKWRWYHFDGTDWDQSRSLSRIYKFKSTGKAWDSEVSGENGNYDYLMYADVDFEHPEVR
QEMKNWGKWYADSLGLDGFRLDAVKHINHSYLKEWVTSVRQATGKEMFTVAEYWKNDLGAINDYLAKTGYTHSVFDVPLH
YNFQAAGNGGGYYDMRNILKGTVVEQHPTLAVTIVDNHDSQPGQSLESTVANWFKPLAYATIMTRGQGYPALFYGDYYGT
KGTTNREIPNMSASLQPILKARKDFAYGTQHDYINHQDVIGWTREGVTDRTKSGLATILSDGPGGSKWMYVGKQNAGEVW
KDMTGNNGRLVTINADGWGEFFVNGGSVSIYTQQ

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1788236, Length=491, Percent_Identity=40.5295315682281, Blast_Score=377, Evalue=1e-105,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013776
- InterPro:   IPR015237
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF09154 DUF1939 [H]

EC number: =3.2.1.1 [H]

Molecular weight: Translated: 57511; Mature: 57511

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKRQGIAVLAGVTSIALLSGQPVAQAATPQNGTMMQYFEWYVPNDGQHWNRLSNDSQH
CCCCCCCCEEEEHHHHHHEECCCCCHHCCCCCCCCEEEEEEEECCCCCCHHHHCCCCHHH
LKDIGISTVWIPPAYKGTSQNDVGYGAYDLYDLGEFNQKGTTRTKYGTKAQLQSAISNLR
HHHCCCEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHC
GKGIGVYGDVVMNHKGGADYTESVQAVEVNPSNRNQETSGEYAISAWTGFNFAGRNNTYS
CCCCEEEEEEEEECCCCCCHHHCEEEEEECCCCCCCCCCCCEEEEEEECCEECCCCCCCC
PFKWRWYHFDGTDWDQSRSLSRIYKFKSTGKAWDSEVSGENGNYDYLMYADVDFEHPEVR
CCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHH
QEMKNWGKWYADSLGLDGFRLDAVKHINHSYLKEWVTSVRQATGKEMFTVAEYWKNDLGA
HHHHHHHHHHHHHCCCCCEEHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
INDYLAKTGYTHSVFDVPLHYNFQAAGNGGGYYDMRNILKGTVVEQHPTLAVTIVDNHDS
HHHHHHHCCCCCEEEECCEEEEEEECCCCCCHHHHHHHHHCCEEECCCCEEEEEEECCCC
QPGQSLESTVANWFKPLAYATIMTRGQGYPALFYGDYYGTKGTTNREIPNMSASLQPILK
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHH
ARKDFAYGTQHDYINHQDVIGWTREGVTDRTKSGLATILSDGPGGSKWMYVGKQNAGEVW
HHHHHCCCCCCCCCCCCHHCCCCCCCCCHHHHCCCEEEECCCCCCCEEEEEECCCCHHHH
KDMTGNNGRLVTINADGWGEFFVNGGSVSIYTQQ
HHCCCCCCEEEEEECCCCEEEEEECCEEEEEECC
>Mature Secondary Structure
MLKKRQGIAVLAGVTSIALLSGQPVAQAATPQNGTMMQYFEWYVPNDGQHWNRLSNDSQH
CCCCCCCCEEEEHHHHHHEECCCCCHHCCCCCCCCEEEEEEEECCCCCCHHHHCCCCHHH
LKDIGISTVWIPPAYKGTSQNDVGYGAYDLYDLGEFNQKGTTRTKYGTKAQLQSAISNLR
HHHCCCEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHC
GKGIGVYGDVVMNHKGGADYTESVQAVEVNPSNRNQETSGEYAISAWTGFNFAGRNNTYS
CCCCEEEEEEEEECCCCCCHHHCEEEEEECCCCCCCCCCCCEEEEEEECCEECCCCCCCC
PFKWRWYHFDGTDWDQSRSLSRIYKFKSTGKAWDSEVSGENGNYDYLMYADVDFEHPEVR
CCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHH
QEMKNWGKWYADSLGLDGFRLDAVKHINHSYLKEWVTSVRQATGKEMFTVAEYWKNDLGA
HHHHHHHHHHHHHCCCCCEEHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
INDYLAKTGYTHSVFDVPLHYNFQAAGNGGGYYDMRNILKGTVVEQHPTLAVTIVDNHDS
HHHHHHHCCCCCEEEECCEEEEEEECCCCCCHHHHHHHHHCCEEECCCCEEEEEEECCCC
QPGQSLESTVANWFKPLAYATIMTRGQGYPALFYGDYYGTKGTTNREIPNMSASLQPILK
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHH
ARKDFAYGTQHDYINHQDVIGWTREGVTDRTKSGLATILSDGPGGSKWMYVGKQNAGEVW
HHHHHCCCCCCCCCCCCHHCCCCCCCCCHHHHCCCEEEECCCCCCCEEEEEECCCCHHHH
KDMTGNNGRLVTINADGWGEFFVNGGSVSIYTQQ
HHCCCCCCEEEEEECCCCEEEEEECCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6185474; 6170539; 2830166; 6156671; 10924103 [H]