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The map label for this gene is glyA [H]

Identifier: 229917051

GI number: 229917051

Start: 1298167

End: 1299420

Strand: Reverse

Name: glyA [H]

Synonym: EAT1b_1325

Alternate gene names: 229917051

Gene position: 1299420-1298167 (Counterclockwise)

Preceding gene: 229917059

Following gene: 229917050

Centisome position: 43.32

GC content: 52.47

Gene sequence:

>1254_bases
ATGGTAAACACGACGAAGTTGAAAGTGCAGGATGCGGAATTGTTTGAGGCAATGCAACACGAGCTCGGTCGTCAGCGCGA
CAACATCGAACTCATCGCTTCAGAAAACTTTGTTTCAGAAGCGGTCATGGAAGCGCAAGGAGGCGTATTGACGAACAAAT
ATGCCGAAGGATATCCGGGACGCCGTTACTATGGTGGCTGTGAGTTTGTCGACGTCGCTGAGAACTTGGCGCGTGACCGT
GCGAAAGAATTGTTCGGTGCAGAACATGCGAACGTACAACCACACTCGGGTGCACAAGCGAACATGGCGGTCTACTTCAC
GGTGCTTGAAGCAGGAGATACGGTTCTCGGAATGAACCTGTCTCATGGTGGTCACTTGACGCACGGGAGTCCGGTCAACT
TCTCAGGTGTGCAATATAACTTCGTCGAATACGGTGTAGACAAAGAGACAGAACACATCGATTATGACGTCGTCGCTGCA
CTTGCTAAAGAACATAAGCCGAAATTGATTGTCGCGGGAGCATCAGCATACCCACGTACGATCGACTTCGCGAAATTCCG
TGAGATCGCGGACAGTGTCGATGCTTACCTTATGGTCGATATGGCGCACATCGCTGGACTCGTGGCAGCGGGGCTTCACC
CGAACCCGGTCGAGCATGCGCACTTCGTCACGACGACGACGCATAAGACGCTTCGTGGACCACGTGGCGGAATGATTCTT
TGTAAAGAAGAGTTCGCCAAAGCGATCGACAAGTCGATTTTCCCAGGGATTCAAGGTGGACCACTCATGCACGTCATCGC
AGCGAAAGCGGTCGCGTTCGGTGAGGCACTCCAACCGGAATTCAAAGATTACCAACGTCAAGTCATTGCGAATGCGCAAG
CACTTGCGGCAGGTCTTGAAGAAGAGGGACTCCGAATCGTATCGGGCGGAACGGACAATCACCTCTTGCTCGTCGATCTT
CGAGGAATCGACATCACAGGTAAAGCGGCTGAGCATGCACTAGATGCAGCGGGAATCACAGTCAATAAAAACACGATTCC
ATTCGACCCGGCATCACCGTTCGTCACAAGCGGAATCCGTCTCGGAACGGCTGCGATGACGACTCGTGGTTTCAAAGAAA
CGGATATGAAAGAAGTGGCACGTCTCATCGGGCGTGTGCTCAAACAACATGAAGACGAGGCGGTAATCGCGGAAGCACTT
CAAGACGTGCGATTGTTAACGGCGAAGTTCCCGCTTTACCCTGAACGAGGCTGA

Upstream 100 bases:

>100_bases
ATGATACCCAACTTTTCATCAGATTATTGCGATACCCAAAAAAACAAGCGTGTGGTACGATGGGGGTGTTGATTTTTAGA
CATTTAGAGGGGGTCTTCAC

Downstream 100 bases:

>100_bases
GTCAATGGATATTCGGACACTAGCAGAACGAGAGCTATCCGAACTTCCATTGTTCGATATGACAGAATGGTTGGCTCGTA
AAGGGGAATACGATCCGTCT

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase [H]

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MVNTTKLKVQDAELFEAMQHELGRQRDNIELIASENFVSEAVMEAQGGVLTNKYAEGYPGRRYYGGCEFVDVAENLARDR
AKELFGAEHANVQPHSGAQANMAVYFTVLEAGDTVLGMNLSHGGHLTHGSPVNFSGVQYNFVEYGVDKETEHIDYDVVAA
LAKEHKPKLIVAGASAYPRTIDFAKFREIADSVDAYLMVDMAHIAGLVAAGLHPNPVEHAHFVTTTTHKTLRGPRGGMIL
CKEEFAKAIDKSIFPGIQGGPLMHVIAAKAVAFGEALQPEFKDYQRQVIANAQALAAGLEEEGLRIVSGGTDNHLLLVDL
RGIDITGKAAEHALDAAGITVNKNTIPFDPASPFVTSGIRLGTAAMTTRGFKETDMKEVARLIGRVLKQHEDEAVIAEAL
QDVRLLTAKFPLYPERG

Sequences:

>Translated_417_residues
MVNTTKLKVQDAELFEAMQHELGRQRDNIELIASENFVSEAVMEAQGGVLTNKYAEGYPGRRYYGGCEFVDVAENLARDR
AKELFGAEHANVQPHSGAQANMAVYFTVLEAGDTVLGMNLSHGGHLTHGSPVNFSGVQYNFVEYGVDKETEHIDYDVVAA
LAKEHKPKLIVAGASAYPRTIDFAKFREIADSVDAYLMVDMAHIAGLVAAGLHPNPVEHAHFVTTTTHKTLRGPRGGMIL
CKEEFAKAIDKSIFPGIQGGPLMHVIAAKAVAFGEALQPEFKDYQRQVIANAQALAAGLEEEGLRIVSGGTDNHLLLVDL
RGIDITGKAAEHALDAAGITVNKNTIPFDPASPFVTSGIRLGTAAMTTRGFKETDMKEVARLIGRVLKQHEDEAVIAEAL
QDVRLLTAKFPLYPERG
>Mature_417_residues
MVNTTKLKVQDAELFEAMQHELGRQRDNIELIASENFVSEAVMEAQGGVLTNKYAEGYPGRRYYGGCEFVDVAENLARDR
AKELFGAEHANVQPHSGAQANMAVYFTVLEAGDTVLGMNLSHGGHLTHGSPVNFSGVQYNFVEYGVDKETEHIDYDVVAA
LAKEHKPKLIVAGASAYPRTIDFAKFREIADSVDAYLMVDMAHIAGLVAAGLHPNPVEHAHFVTTTTHKTLRGPRGGMIL
CKEEFAKAIDKSIFPGIQGGPLMHVIAAKAVAFGEALQPEFKDYQRQVIANAQALAAGLEEEGLRIVSGGTDNHLLLVDL
RGIDITGKAAEHALDAAGITVNKNTIPFDPASPFVTSGIRLGTAAMTTRGFKETDMKEVARLIGRVLKQHEDEAVIAEAL
QDVRLLTAKFPLYPERG

Specific function: Interconversion of serine and glycine [H]

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family [H]

Homologues:

Organism=Homo sapiens, GI19923315, Length=401, Percent_Identity=46.6334164588529, Blast_Score=353, Evalue=1e-97,
Organism=Homo sapiens, GI261862352, Length=401, Percent_Identity=46.6334164588529, Blast_Score=353, Evalue=1e-97,
Organism=Homo sapiens, GI261862350, Length=401, Percent_Identity=46.6334164588529, Blast_Score=353, Evalue=1e-97,
Organism=Homo sapiens, GI261862348, Length=401, Percent_Identity=46.6334164588529, Blast_Score=353, Evalue=1e-97,
Organism=Homo sapiens, GI261862346, Length=397, Percent_Identity=46.5994962216625, Blast_Score=342, Evalue=6e-94,
Organism=Homo sapiens, GI22547186, Length=403, Percent_Identity=47.394540942928, Blast_Score=340, Evalue=1e-93,
Organism=Homo sapiens, GI22547189, Length=388, Percent_Identity=46.6494845360825, Blast_Score=313, Evalue=2e-85,
Organism=Escherichia coli, GI1788902, Length=406, Percent_Identity=64.5320197044335, Blast_Score=508, Evalue=1e-145,
Organism=Caenorhabditis elegans, GI25144732, Length=423, Percent_Identity=46.5721040189125, Blast_Score=358, Evalue=4e-99,
Organism=Caenorhabditis elegans, GI25144729, Length=423, Percent_Identity=46.5721040189125, Blast_Score=357, Evalue=5e-99,
Organism=Saccharomyces cerevisiae, GI6319739, Length=406, Percent_Identity=48.0295566502463, Blast_Score=380, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6323087, Length=409, Percent_Identity=44.4987775061125, Blast_Score=328, Evalue=9e-91,
Organism=Drosophila melanogaster, GI24640005, Length=407, Percent_Identity=47.9115479115479, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI221329721, Length=407, Percent_Identity=47.9115479115479, Blast_Score=374, Evalue=1e-104,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798 [H]

Pfam domain/function: PF00464 SHMT [H]

EC number: =2.1.2.1 [H]

Molecular weight: Translated: 45202; Mature: 45202

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNTTKLKVQDAELFEAMQHELGRQRDNIELIASENFVSEAVMEAQGGVLTNKYAEGYPG
CCCCEEEEEEHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCEEECHHHCCCCC
RRYYGGCEFVDVAENLARDRAKELFGAEHANVQPHSGAQANMAVYFTVLEAGDTVLGMNL
CEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCEEEEEEE
SHGGHLTHGSPVNFSGVQYNFVEYGVDKETEHIDYDVVAALAKEHKPKLIVAGASAYPRT
CCCCCCCCCCCCCCCCEEEEHEEECCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCC
IDFAKFREIADSVDAYLMVDMAHIAGLVAAGLHPNPVEHAHFVTTTTHKTLRGPRGGMIL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHCCCCCCEEE
CKEEFAKAIDKSIFPGIQGGPLMHVIAAKAVAFGEALQPEFKDYQRQVIANAQALAAGLE
EHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCHH
EEGLRIVSGGTDNHLLLVDLRGIDITGKAAEHALDAAGITVNKNTIPFDPASPFVTSGIR
HCCEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHCCEEECCCCCCCCCCCCHHHCCCE
LGTAAMTTRGFKETDMKEVARLIGRVLKQHEDEAVIAEALQDVRLLTAKFPLYPERG
ECHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MVNTTKLKVQDAELFEAMQHELGRQRDNIELIASENFVSEAVMEAQGGVLTNKYAEGYPG
CCCCEEEEEEHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCEEECHHHCCCCC
RRYYGGCEFVDVAENLARDRAKELFGAEHANVQPHSGAQANMAVYFTVLEAGDTVLGMNL
CEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCEEEEEEE
SHGGHLTHGSPVNFSGVQYNFVEYGVDKETEHIDYDVVAALAKEHKPKLIVAGASAYPRT
CCCCCCCCCCCCCCCCEEEEHEEECCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCC
IDFAKFREIADSVDAYLMVDMAHIAGLVAAGLHPNPVEHAHFVTTTTHKTLRGPRGGMIL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHCCCCCCEEE
CKEEFAKAIDKSIFPGIQGGPLMHVIAAKAVAFGEALQPEFKDYQRQVIANAQALAAGLE
EHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCHH
EEGLRIVSGGTDNHLLLVDLRGIDITGKAAEHALDAAGITVNKNTIPFDPASPFVTSGIR
HCCEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHCCEEECCCCCCCCCCCCHHHCCCE
LGTAAMTTRGFKETDMKEVARLIGRVLKQHEDEAVIAEALQDVRLLTAKFPLYPERG
ECHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA