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The map label for this gene is yisY [H]

Identifier: 229916695

GI number: 229916695

Start: 913987

End: 914787

Strand: Reverse

Name: yisY [H]

Synonym: EAT1b_0968

Alternate gene names: 229916695

Gene position: 914787-913987 (Counterclockwise)

Preceding gene: 229916696

Following gene: 229916694

Centisome position: 30.49

GC content: 49.69

Gene sequence:

>801_bases
ATGGGATATATTACAGCACATGACGGTACAAATCTATACGTAGAGGATGTCGGGTCTGGGGAACCGATTGTCTTCTTGCA
CGGCTGGCCAGCAAACAACAATATGTTCGAGTATCAGAAGAATGCGCTCGTCGAAGCGGGATATCGCTACATCGGCATTG
ATTTCAGAGGATACGGGAAATCTGATGCCCCGGCGACAGGTTATGACTATGAGACAATGGCGTCTGATGTGCATATGGTC
GTTTACGGACTTGGCTTAAAAGATTTCACGCTCGTCGGTTTTTCAATGGGTGGGGCGATTGCAGTTCGCTACGCTGCCGA
CTATAAAGAGGATGGTCTGAAAAAGTTAGTGTTAGCGGGAGCAGCAGCACCAATCTTCACACAGCGTGCAGATTATCCAT
ATGGAATGACAACGGACGAAGTCGACGCGTTGATTGACGATACACGGGCCGACCGACCGAAGATGCTCGAAGGGTTCGGA
GAAATCTTCTTCGAGAAAGAACATTCGAAACCGATGCAAAACTGGTTCCACCATCTCGCGCTCGTCGCGTCGAGCCATGG
GACGATTGCCTCGGCACAGGCGTTACGTGACGAAGATTTACGAGATGCCCTGCCGAAAATTGATGTCGAGACGTTGATCA
TCCATGGAGTACATGACAAGATCTGTCCTTTCGCATTCGCCGAGCAGATGGAGCAAGGCATCTCGAACGCGCGAATCGAG
CGCTTTGAAGAGAGTGGACACGGTACGGTGCTGGATGAACGAGAGAAATTTAACGATACGTTGTTGCAGTTTGTTCAATA
A

Upstream 100 bases:

>100_bases
AGCGCTCTGGCGCGACACATAAGAGGGTTTCTCGAAGTTGGAATAGGGAACAATGAAGTTGTGAGTGTTTTCGCTAAATA
ATGACTGTGGAGGTTGGATG

Downstream 100 bases:

>100_bases
TCGATGGCCTCCGAATCGGGGGCTTTTTGTATGTCGATAAGGGTAAGAACCAACGAAAACGGGTATAATTCAATTGGAGT
TCCCTTCCATTTTTTGAATC

Product: alpha/beta hydrolase fold protein

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MGYITAHDGTNLYVEDVGSGEPIVFLHGWPANNNMFEYQKNALVEAGYRYIGIDFRGYGKSDAPATGYDYETMASDVHMV
VYGLGLKDFTLVGFSMGGAIAVRYAADYKEDGLKKLVLAGAAAPIFTQRADYPYGMTTDEVDALIDDTRADRPKMLEGFG
EIFFEKEHSKPMQNWFHHLALVASSHGTIASAQALRDEDLRDALPKIDVETLIIHGVHDKICPFAFAEQMEQGISNARIE
RFEESGHGTVLDEREKFNDTLLQFVQ

Sequences:

>Translated_266_residues
MGYITAHDGTNLYVEDVGSGEPIVFLHGWPANNNMFEYQKNALVEAGYRYIGIDFRGYGKSDAPATGYDYETMASDVHMV
VYGLGLKDFTLVGFSMGGAIAVRYAADYKEDGLKKLVLAGAAAPIFTQRADYPYGMTTDEVDALIDDTRADRPKMLEGFG
EIFFEKEHSKPMQNWFHHLALVASSHGTIASAQALRDEDLRDALPKIDVETLIIHGVHDKICPFAFAEQMEQGISNARIE
RFEESGHGTVLDEREKFNDTLLQFVQ
>Mature_265_residues
GYITAHDGTNLYVEDVGSGEPIVFLHGWPANNNMFEYQKNALVEAGYRYIGIDFRGYGKSDAPATGYDYETMASDVHMVV
YGLGLKDFTLVGFSMGGAIAVRYAADYKEDGLKKLVLAGAAAPIFTQRADYPYGMTTDEVDALIDDTRADRPKMLEGFGE
IFFEKEHSKPMQNWFHHLALVASSHGTIASAQALRDEDLRDALPKIDVETLIIHGVHDKICPFAFAEQMEQGISNARIER
FEESGHGTVLDEREKFNDTLLQFVQ

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily [H]

Homologues:

Organism=Homo sapiens, GI27597073, Length=111, Percent_Identity=32.4324324324324, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI87081721, Length=275, Percent_Identity=26.9090909090909, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 29635; Mature: 29503

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGYITAHDGTNLYVEDVGSGEPIVFLHGWPANNNMFEYQKNALVEAGYRYIGIDFRGYGK
CCEEEECCCCEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCC
SDAPATGYDYETMASDVHMVVYGLGLKDFTLVGFSMGGAIAVRYAADYKEDGLKKLVLAG
CCCCCCCCCHHHHHCCCEEEEEECCCCCEEEEEEECCCEEEEEEECCCCHHHHHHHHHHC
AAAPIFTQRADYPYGMTTDEVDALIDDTRADRPKMLEGFGEIFFEKEHSKPMQNWFHHLA
CCCHHHHCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHEECCCCCHHHHHHHHHH
LVASSHGTIASAQALRDEDLRDALPKIDVETLIIHGVHDKICPFAFAEQMEQGISNARIE
HHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
RFEESGHGTVLDEREKFNDTLLQFVQ
HHHHCCCCCEECCHHHHHHHHHHHCC
>Mature Secondary Structure 
GYITAHDGTNLYVEDVGSGEPIVFLHGWPANNNMFEYQKNALVEAGYRYIGIDFRGYGK
CEEEECCCCEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCC
SDAPATGYDYETMASDVHMVVYGLGLKDFTLVGFSMGGAIAVRYAADYKEDGLKKLVLAG
CCCCCCCCCHHHHHCCCEEEEEECCCCCEEEEEEECCCEEEEEEECCCCHHHHHHHHHHC
AAAPIFTQRADYPYGMTTDEVDALIDDTRADRPKMLEGFGEIFFEKEHSKPMQNWFHHLA
CCCHHHHCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHEECCCCCHHHHHHHHHH
LVASSHGTIASAQALRDEDLRDALPKIDVETLIIHGVHDKICPFAFAEQMEQGISNARIE
HHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
RFEESGHGTVLDEREKFNDTLLQFVQ
HHHHCCCCCEECCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377 [H]