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The map label for this gene is glpF [H]

Identifier: 229916614

GI number: 229916614

Start: 840432

End: 841139

Strand: Direct

Name: glpF [H]

Synonym: EAT1b_0886

Alternate gene names: 229916614

Gene position: 840432-841139 (Clockwise)

Preceding gene: 229916610

Following gene: 229916615

Centisome position: 28.02

GC content: 51.84

Gene sequence:

>708_bases
ATGTCAGCATTTGTCAGTGAAATTATTGGTACGATGCTATTGATTCTGCTCGGTAACGGTGTCGTCGCCGGCGTCGTCCT
CCGCCATTCAAAAGCGGAGAGCGCAGGTTGGGTCGTCATCACCTTTGGTTGGGGATTCGCTGTTATGATTGGGGTATACG
CAGCTGGAATTTATGGAGGAGCGCATTTGAATCCTGCTGTCACCGTCGGGTTGGCTACAGCAGGCTCATTCCCTTGGGCT
GATGTGCCGGCTTACATCTTGGCGCAATTTATCGGGGCGTTCATCGGGGCCGTCCTCGTATTTCTACACTACAAGCCACA
TTATGATGTGACCGAAGATGCGGGACTCAAGTTGGCCACGTTCTCCACTGCGCCAGCCATTCGTAATACGCCATTTAACT
TGATCTCGGAAATCATCGGTACGTTCGTCCTCGTGTTTGGGATTTTATCATTTGGAGCCAACACGTTCACAGACGGCTTG
AATCCGCTCATCGTAGCGTTTCTCGTTGTATCAATTGGTATGTCTCTTGGTGGACCTACCGGTTATGCGATCAACCCGGC
ACGTGACCTTGGACCGCGTATCGCTCACGCCGTGCTCCCGATTAAAGGAAAAGGCACATCAGACTGGGGTTACTCATGGA
TTCCAGTTGTCGGTCCGATTATCGGTGCTGTCATCGCCGCACTCGTTTACGGCATTCTCGTCCCTTAA

Upstream 100 bases:

>100_bases
TAAAGTAGGATTTTTGAAAAACACTGTTTTAAAACGCTTACATTTTGGGCATATAATGAAGTGGTAAGTCAATTTGATAA
GGGAGAAAGGGGAGTATCAC

Downstream 100 bases:

>100_bases
CACATAATTTTGAAGGAGGAAATCACATATGGCGAAAGAAAAACGTTATATTTTAGCATTGGACCAAGGAACGACAAGTT
CACGGGCAATCATTTTTGAT

Product: MIP family channel protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 235; Mature: 234

Protein sequence:

>235_residues
MSAFVSEIIGTMLLILLGNGVVAGVVLRHSKAESAGWVVITFGWGFAVMIGVYAAGIYGGAHLNPAVTVGLATAGSFPWA
DVPAYILAQFIGAFIGAVLVFLHYKPHYDVTEDAGLKLATFSTAPAIRNTPFNLISEIIGTFVLVFGILSFGANTFTDGL
NPLIVAFLVVSIGMSLGGPTGYAINPARDLGPRIAHAVLPIKGKGTSDWGYSWIPVVGPIIGAVIAALVYGILVP

Sequences:

>Translated_235_residues
MSAFVSEIIGTMLLILLGNGVVAGVVLRHSKAESAGWVVITFGWGFAVMIGVYAAGIYGGAHLNPAVTVGLATAGSFPWA
DVPAYILAQFIGAFIGAVLVFLHYKPHYDVTEDAGLKLATFSTAPAIRNTPFNLISEIIGTFVLVFGILSFGANTFTDGL
NPLIVAFLVVSIGMSLGGPTGYAINPARDLGPRIAHAVLPIKGKGTSDWGYSWIPVVGPIIGAVIAALVYGILVP
>Mature_234_residues
SAFVSEIIGTMLLILLGNGVVAGVVLRHSKAESAGWVVITFGWGFAVMIGVYAAGIYGGAHLNPAVTVGLATAGSFPWAD
VPAYILAQFIGAFIGAVLVFLHYKPHYDVTEDAGLKLATFSTAPAIRNTPFNLISEIIGTFVLVFGILSFGANTFTDGLN
PLIVAFLVVSIGMSLGGPTGYAINPARDLGPRIAHAVLPIKGKGTSDWGYSWIPVVGPIIGAVIAALVYGILVP

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4826645, Length=245, Percent_Identity=35.5102040816327, Blast_Score=126, Evalue=1e-29,
Organism=Homo sapiens, GI157266307, Length=244, Percent_Identity=38.5245901639344, Blast_Score=125, Evalue=3e-29,
Organism=Homo sapiens, GI22538420, Length=246, Percent_Identity=34.1463414634146, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI4502187, Length=248, Percent_Identity=32.258064516129, Blast_Score=107, Evalue=7e-24,
Organism=Homo sapiens, GI310133356, Length=166, Percent_Identity=33.7349397590361, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI310114181, Length=166, Percent_Identity=33.7349397590361, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI4502181, Length=195, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI4755125, Length=195, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1790362, Length=250, Percent_Identity=34.8, Blast_Score=101, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI71994009, Length=241, Percent_Identity=32.7800829875519, Blast_Score=111, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17544068, Length=249, Percent_Identity=33.7349397590361, Blast_Score=107, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI71992966, Length=245, Percent_Identity=32.6530612244898, Blast_Score=104, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17531431, Length=245, Percent_Identity=32.2448979591837, Blast_Score=103, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17531429, Length=245, Percent_Identity=32.2448979591837, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI32564052, Length=238, Percent_Identity=31.9327731092437, Blast_Score=99, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17533613, Length=238, Percent_Identity=31.9327731092437, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17558372, Length=234, Percent_Identity=32.0512820512821, Blast_Score=91, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71992961, Length=233, Percent_Identity=31.7596566523605, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71993722, Length=234, Percent_Identity=30.7692307692308, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6321054, Length=206, Percent_Identity=35.9223300970874, Blast_Score=118, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6322985, Length=291, Percent_Identity=29.553264604811, Blast_Score=90, Evalue=3e-19,
Organism=Drosophila melanogaster, GI45550503, Length=233, Percent_Identity=29.6137339055794, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24652747, Length=230, Percent_Identity=28.695652173913, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI45551084, Length=230, Percent_Identity=28.695652173913, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 24370; Mature: 24239

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAFVSEIIGTMLLILLGNGVVAGVVLRHSKAESAGWVVITFGWGFAVMIGVYAAGIYGG
CHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEEEEECHHHHHHHHHHHHHHCCC
AHLNPAVTVGLATAGSFPWADVPAYILAQFIGAFIGAVLVFLHYKPHYDVTEDAGLKLAT
CCCCHHHEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEE
FSTAPAIRNTPFNLISEIIGTFVLVFGILSFGANTFTDGLNPLIVAFLVVSIGMSLGGPT
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHCCCCC
GYAINPARDLGPRIAHAVLPIKGKGTSDWGYSWIPVVGPIIGAVIAALVYGILVP
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SAFVSEIIGTMLLILLGNGVVAGVVLRHSKAESAGWVVITFGWGFAVMIGVYAAGIYGG
HHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEEEEECHHHHHHHHHHHHHHCCC
AHLNPAVTVGLATAGSFPWADVPAYILAQFIGAFIGAVLVFLHYKPHYDVTEDAGLKLAT
CCCCHHHEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEE
FSTAPAIRNTPFNLISEIIGTFVLVFGILSFGANTFTDGLNPLIVAFLVVSIGMSLGGPT
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHCCCCC
GYAINPARDLGPRIAHAVLPIKGKGTSDWGYSWIPVVGPIIGAVIAALVYGILVP
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]