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The map label for this gene is glpK [H]

Identifier: 229916615

GI number: 229916615

Start: 841168

End: 842685

Strand: Direct

Name: glpK [H]

Synonym: EAT1b_0887

Alternate gene names: 229916615

Gene position: 841168-842685 (Clockwise)

Preceding gene: 229916614

Following gene: 229916627

Centisome position: 28.04

GC content: 50.99

Gene sequence:

>1518_bases
ATGGCGAAAGAAAAACGTTATATTTTAGCATTGGACCAAGGAACGACAAGTTCACGGGCAATCATTTTTGATCATGATGG
AAAAATTGTCACCGTCGCACAGCGCGAATTCAAACAATACTTCCCAAAACCAGGCTGGGTCGAGCACAACGCCAATGAGA
TTTGGGGCTCTGTCCTCGCCGTTATGGCGGAAGCGTTCGGTTCTGCTGATATCGACCCGAATGAGATTGCTGGAATCGGG
ATCACAAACCAACGTGAAACAGCTGTCGTCTGGGAAAAAGAAACAGGACGCCCTGTCTATAACGCCGTTGTCTGGCAATC
CCGTCAAACCGCCGACATCTGTGAAGAGCTGAAAGCAGCCGGTCATTCCGATATGGTCCGCGACAAAACAGGTCTCCTCA
TCGATGCCTACTTCTCAGGAACAAAAGTCAAATGGATTCTTGACAACGTCGAGGGTGCACGTGAAAAAGCTGAGAACGGC
GAGCTCCTCTTCGGGACAATCGACACGTGGCTCATTTGGAAACTCTCTGGTGGAAAAGCACACGTAACCGATTACTCGAA
CGCATCGCGTACACTCATGTACAATATCTACGAGCAGAAGTGGGATGACGAGTTACTCGACATCTTGACAGTGCCTAAAT
CCATGCTTCCTGAAGTCAAACCGTCAAGTGAAGTCTATGCGAACACGATTCCATACCACTTCTTCGGATTCGAAGTCCCG
ATTGCCGGAGCAGCAGGTGACCAACAGGCCGCCTTGTTCGGACAAACGTGCTTCGAAAAAGGTGAGGCGAAAAACACGTA
CGGAACAGGTTGTTTCATGCTCATGAACACGGGAGAAGAAGCCGTCAAATCCGATCACGGTCTCTTGACGACAATCGCAT
GGGGCTATAACGGGAAAGTAGAATATGCGCTCGAAGGATCAATCTTCGTCGCCGGTTCTGCGATCCAGTGGCTCCGTGAC
GGATTGCGGATGTTGAAATCAGCGAAAGACTCGGAACAGTATGCGACACGGATCGAATCAACTGACGGTGTCTATGTCGT
TCCTGCCTTCGTTGGTCTCGGTGCCCCGTACTGGAACTCTGACGTCCGTGGTGCCATCTTCGGTCTCACGCGTGGGACAG
AGAAAGAACAGATGGTCCGTGCGACAATCGAGTCACTCGCTTACCAGACACGTGATGTCCTCACGTCGATGGAGAGTGAT
TCAGGCATCAACTTGAAGACGCTCCGCGTCGATGGTGGGGCCGTCAGCAACAACTTCCTCATGCAGTTCCAATCGGACAT
TCTCAATGTTCCGGTCGAACGTCCTGAAGTGAGTGAAACGACTGCACTCGGTGCCGCCTACTTGGCGGGTCTCGCCGTTG
GATTCTGGAAAGACCAAGCCGAAGTCAAAGACCAGTGGAAACTCGACAAGAAGTTCGATCCGAACATGGACGAGTCTCAC
CGTGAAGCCCTCTACAAAGGATGGCAACACGCAGTCGAAGCCACAATGGCATTCAAACCTTCAAAACTTGAAGTATAA

Upstream 100 bases:

>100_bases
TGGATTCCAGTTGTCGGTCCGATTATCGGTGCTGTCATCGCCGCACTCGTTTACGGCATTCTCGTCCCTTAACACATAAT
TTTGAAGGAGGAAATCACAT

Downstream 100 bases:

>100_bases
GACGAATGGGCATCCTTACGGGTGCCCATTTTGTATGCAAAAAAGAGTATCCAAAACGGATACTCTTACAGTTTACGTAA
GCGAACTCTCGGGCTCGTCG

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 505; Mature: 504

Protein sequence:

>505_residues
MAKEKRYILALDQGTTSSRAIIFDHDGKIVTVAQREFKQYFPKPGWVEHNANEIWGSVLAVMAEAFGSADIDPNEIAGIG
ITNQRETAVVWEKETGRPVYNAVVWQSRQTADICEELKAAGHSDMVRDKTGLLIDAYFSGTKVKWILDNVEGAREKAENG
ELLFGTIDTWLIWKLSGGKAHVTDYSNASRTLMYNIYEQKWDDELLDILTVPKSMLPEVKPSSEVYANTIPYHFFGFEVP
IAGAAGDQQAALFGQTCFEKGEAKNTYGTGCFMLMNTGEEAVKSDHGLLTTIAWGYNGKVEYALEGSIFVAGSAIQWLRD
GLRMLKSAKDSEQYATRIESTDGVYVVPAFVGLGAPYWNSDVRGAIFGLTRGTEKEQMVRATIESLAYQTRDVLTSMESD
SGINLKTLRVDGGAVSNNFLMQFQSDILNVPVERPEVSETTALGAAYLAGLAVGFWKDQAEVKDQWKLDKKFDPNMDESH
REALYKGWQHAVEATMAFKPSKLEV

Sequences:

>Translated_505_residues
MAKEKRYILALDQGTTSSRAIIFDHDGKIVTVAQREFKQYFPKPGWVEHNANEIWGSVLAVMAEAFGSADIDPNEIAGIG
ITNQRETAVVWEKETGRPVYNAVVWQSRQTADICEELKAAGHSDMVRDKTGLLIDAYFSGTKVKWILDNVEGAREKAENG
ELLFGTIDTWLIWKLSGGKAHVTDYSNASRTLMYNIYEQKWDDELLDILTVPKSMLPEVKPSSEVYANTIPYHFFGFEVP
IAGAAGDQQAALFGQTCFEKGEAKNTYGTGCFMLMNTGEEAVKSDHGLLTTIAWGYNGKVEYALEGSIFVAGSAIQWLRD
GLRMLKSAKDSEQYATRIESTDGVYVVPAFVGLGAPYWNSDVRGAIFGLTRGTEKEQMVRATIESLAYQTRDVLTSMESD
SGINLKTLRVDGGAVSNNFLMQFQSDILNVPVERPEVSETTALGAAYLAGLAVGFWKDQAEVKDQWKLDKKFDPNMDESH
REALYKGWQHAVEATMAFKPSKLEV
>Mature_504_residues
AKEKRYILALDQGTTSSRAIIFDHDGKIVTVAQREFKQYFPKPGWVEHNANEIWGSVLAVMAEAFGSADIDPNEIAGIGI
TNQRETAVVWEKETGRPVYNAVVWQSRQTADICEELKAAGHSDMVRDKTGLLIDAYFSGTKVKWILDNVEGAREKAENGE
LLFGTIDTWLIWKLSGGKAHVTDYSNASRTLMYNIYEQKWDDELLDILTVPKSMLPEVKPSSEVYANTIPYHFFGFEVPI
AGAAGDQQAALFGQTCFEKGEAKNTYGTGCFMLMNTGEEAVKSDHGLLTTIAWGYNGKVEYALEGSIFVAGSAIQWLRDG
LRMLKSAKDSEQYATRIESTDGVYVVPAFVGLGAPYWNSDVRGAIFGLTRGTEKEQMVRATIESLAYQTRDVLTSMESDS
GINLKTLRVDGGAVSNNFLMQFQSDILNVPVERPEVSETTALGAAYLAGLAVGFWKDQAEVKDQWKLDKKFDPNMDESHR
EALYKGWQHAVEATMAFKPSKLEV

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI189409120, Length=514, Percent_Identity=44.3579766536965, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI4504007, Length=516, Percent_Identity=44.1860465116279, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI42794763, Length=520, Percent_Identity=44.0384615384615, Blast_Score=431, Evalue=1e-121,
Organism=Homo sapiens, GI41393575, Length=506, Percent_Identity=43.4782608695652, Blast_Score=425, Evalue=1e-119,
Organism=Homo sapiens, GI88196792, Length=515, Percent_Identity=32.8155339805825, Blast_Score=309, Evalue=4e-84,
Organism=Escherichia coli, GI1790361, Length=496, Percent_Identity=62.5, Blast_Score=679, Evalue=0.0,
Organism=Escherichia coli, GI1787789, Length=525, Percent_Identity=24.1904761904762, Blast_Score=106, Evalue=5e-24,
Organism=Escherichia coli, GI1790005, Length=525, Percent_Identity=21.7142857142857, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789987, Length=495, Percent_Identity=23.8383838383838, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1789168, Length=480, Percent_Identity=22.2916666666667, Blast_Score=79, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17535599, Length=497, Percent_Identity=40.0402414486922, Blast_Score=379, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI71986140, Length=521, Percent_Identity=24.7600767754319, Blast_Score=187, Evalue=8e-48,
Organism=Caenorhabditis elegans, GI71986147, Length=300, Percent_Identity=29, Blast_Score=134, Evalue=9e-32,
Organism=Saccharomyces cerevisiae, GI6321755, Length=545, Percent_Identity=35.045871559633, Blast_Score=308, Evalue=1e-84,
Organism=Drosophila melanogaster, GI17864214, Length=511, Percent_Identity=41.2915851272016, Blast_Score=387, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24654573, Length=439, Percent_Identity=43.0523917995444, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24655555, Length=496, Percent_Identity=37.7016129032258, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24655560, Length=496, Percent_Identity=37.7016129032258, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24655570, Length=496, Percent_Identity=37.7016129032258, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24655574, Length=496, Percent_Identity=37.7016129032258, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24655565, Length=496, Percent_Identity=37.7016129032258, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI19922058, Length=524, Percent_Identity=30.9160305343511, Blast_Score=284, Evalue=9e-77,
Organism=Drosophila melanogaster, GI24652871, Length=491, Percent_Identity=31.7718940936864, Blast_Score=272, Evalue=4e-73,
Organism=Drosophila melanogaster, GI24656367, Length=493, Percent_Identity=27.1805273833671, Blast_Score=223, Evalue=2e-58,
Organism=Drosophila melanogaster, GI21357867, Length=508, Percent_Identity=26.3779527559055, Blast_Score=217, Evalue=2e-56,
Organism=Drosophila melanogaster, GI24656372, Length=328, Percent_Identity=28.6585365853659, Blast_Score=171, Evalue=1e-42,
Organism=Drosophila melanogaster, GI45552909, Length=292, Percent_Identity=26.7123287671233, Blast_Score=126, Evalue=4e-29,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 56042; Mature: 55911

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00933 FGGY_KINASES_1 ; PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKRYILALDQGTTSSRAIIFDHDGKIVTVAQREFKQYFPKPGWVEHNANEIWGSVLA
CCCCCEEEEEEECCCCCCEEEEEECCCCEEEEHHHHHHHHCCCCCCCCCCHHHHHHHHHH
VMAEAFGSADIDPNEIAGIGITNQRETAVVWEKETGRPVYNAVVWQSRQTADICEELKAA
HHHHHHCCCCCCHHHEEECCCCCCCCEEEEEECCCCCCHHHHHEECCCCHHHHHHHHHHC
GHSDMVRDKTGLLIDAYFSGTKVKWILDNVEGAREKAENGELLFGTIDTWLIWKLSGGKA
CCCCHHHCCCCCEEEEEECCCEEEEEEECCHHHHHHCCCCCEEEEEECEEEEEEECCCEE
HVTDYSNASRTLMYNIYEQKWDDELLDILTVPKSMLPEVKPSSEVYANTIPYHFFGFEVP
EEECCCCCCCEEEEEEHHHCCCHHHHHHHHCCHHHCCCCCCCCCCEECCCCEEEEEEEEE
IAGAAGDQQAALFGQTCFEKGEAKNTYGTGCFMLMNTGEEAVKSDHGLLTTIAWGYNGKV
ECCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCHHHHHCCCCEEEEEEECCCCEE
EYALEGSIFVAGSAIQWLRDGLRMLKSAKDSEQYATRIESTDGVYVVPAFVGLGAPYWNS
EEEECCCEEEECHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEHHHHCCCCCCCC
DVRGAIFGLTRGTEKEQMVRATIESLAYQTRDVLTSMESDSGINLKTLRVDGGAVSNNFL
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHH
MQFQSDILNVPVERPEVSETTALGAAYLAGLAVGFWKDQAEVKDQWKLDKKFDPNMDESH
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCHHH
REALYKGWQHAVEATMAFKPSKLEV
HHHHHHHHHHHHHHHHEECCCCCCC
>Mature Secondary Structure 
AKEKRYILALDQGTTSSRAIIFDHDGKIVTVAQREFKQYFPKPGWVEHNANEIWGSVLA
CCCCEEEEEEECCCCCCEEEEEECCCCEEEEHHHHHHHHCCCCCCCCCCHHHHHHHHHH
VMAEAFGSADIDPNEIAGIGITNQRETAVVWEKETGRPVYNAVVWQSRQTADICEELKAA
HHHHHHCCCCCCHHHEEECCCCCCCCEEEEEECCCCCCHHHHHEECCCCHHHHHHHHHHC
GHSDMVRDKTGLLIDAYFSGTKVKWILDNVEGAREKAENGELLFGTIDTWLIWKLSGGKA
CCCCHHHCCCCCEEEEEECCCEEEEEEECCHHHHHHCCCCCEEEEEECEEEEEEECCCEE
HVTDYSNASRTLMYNIYEQKWDDELLDILTVPKSMLPEVKPSSEVYANTIPYHFFGFEVP
EEECCCCCCCEEEEEEHHHCCCHHHHHHHHCCHHHCCCCCCCCCCEECCCCEEEEEEEEE
IAGAAGDQQAALFGQTCFEKGEAKNTYGTGCFMLMNTGEEAVKSDHGLLTTIAWGYNGKV
ECCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCHHHHHCCCCEEEEEEECCCCEE
EYALEGSIFVAGSAIQWLRDGLRMLKSAKDSEQYATRIESTDGVYVVPAFVGLGAPYWNS
EEEECCCEEEECHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEHHHHCCCCCCCC
DVRGAIFGLTRGTEKEQMVRATIESLAYQTRDVLTSMESDSGINLKTLRVDGGAVSNNFL
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHH
MQFQSDILNVPVERPEVSETTALGAAYLAGLAVGFWKDQAEVKDQWKLDKKFDPNMDESH
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCHHH
REALYKGWQHAVEATMAFKPSKLEV
HHHHHHHHHHHHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA