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The map label for this gene is yvoF [H]

Identifier: 229916606

GI number: 229916606

Start: 830468

End: 830950

Strand: Reverse

Name: yvoF [H]

Synonym: EAT1b_0878

Alternate gene names: 229916606

Gene position: 830950-830468 (Counterclockwise)

Preceding gene: 229916607

Following gene: 229916605

Centisome position: 27.7

GC content: 53.62

Gene sequence:

>483_bases
ATGCGTAAGACGACACGGTATCATATCGAGGGACCGAATCCGCTTTGGCAAATGTATCAGACGATTCGCTTCCTGCGTGT
TTTCAAAAACACGGCGATCGTCCTGATCGGGCGTTACTTCCCGTCGGTGAAGGCGAAACGTTGGCTTTACCGGACGTTCC
TTGGGATGAATATCGGGGAGTATAGTGCCCTTGCTCTCATGGTCACACCGGACGTCATGTTCCCGGAACGCATCACGATC
GGTCGCAATACGGTCATCGGCTTCAATTCGACGATCCTCTGTCATGAGTATCTCGTAGACGAGTATCGGATCGGGGACGT
CATCATCGGTGATGGCGTATTGATTGGTGCGAACGTGACCATCTTGCCGGGTGTGACCATCGGAGACGGTGCCGTCATCG
GTGCGGGAGCGATTGTGCACCGTGACGTCTTACCGGGAGAACGGGTCATCGGTTCGAAGCTTCAATCGTTGACACATATT
TGA

Upstream 100 bases:

>100_bases
TGGGCGCTCAAGGGACGACCGTTCCTCGAATCGCTCGAACCGGACGTCATTATCGACTCGATGGAACATTTCGAGGCATG
GCTAGACGAGGTCAGCATTC

Downstream 100 bases:

>100_bases
CACAATTTTTCGCAAAATGTTTTCATGAGCATTTTGTTCATACAAGATCAAGAATACTTGAGCGGTATCACTTTTTTTAA
AGGAGATGCCGCTTTTTCAT

Product: acetyltransferase

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MRKTTRYHIEGPNPLWQMYQTIRFLRVFKNTAIVLIGRYFPSVKAKRWLYRTFLGMNIGEYSALALMVTPDVMFPERITI
GRNTVIGFNSTILCHEYLVDEYRIGDVIIGDGVLIGANVTILPGVTIGDGAVIGAGAIVHRDVLPGERVIGSKLQSLTHI

Sequences:

>Translated_160_residues
MRKTTRYHIEGPNPLWQMYQTIRFLRVFKNTAIVLIGRYFPSVKAKRWLYRTFLGMNIGEYSALALMVTPDVMFPERITI
GRNTVIGFNSTILCHEYLVDEYRIGDVIIGDGVLIGANVTILPGVTIGDGAVIGAGAIVHRDVLPGERVIGSKLQSLTHI
>Mature_160_residues
MRKTTRYHIEGPNPLWQMYQTIRFLRVFKNTAIVLIGRYFPSVKAKRWLYRTFLGMNIGEYSALALMVTPDVMFPERITI
GRNTVIGFNSTILCHEYLVDEYRIGDVIIGDGVLIGANVTILPGVTIGDGAVIGAGAIVHRDVLPGERVIGSKLQSLTHI

Specific function: May Assist Cellular Detoxification By Acetylating Nonmetabolizable Pyranosides, Thereby Preventing Their Reentry Into The Cell. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 17861; Mature: 17861

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKTTRYHIEGPNPLWQMYQTIRFLRVFKNTAIVLIGRYFPSVKAKRWLYRTFLGMNIGE
CCCCEEEEECCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHCCCCCC
YSALALMVTPDVMFPERITIGRNTVIGFNSTILCHEYLVDEYRIGDVIIGDGVLIGANVT
CEEEEEEEECCCCCCCEEECCCCEEEECCCEEEHHHHHHCCCCCCCEEEECCEEEECCEE
ILPGVTIGDGAVIGAGAIVHRDVLPGERVIGSKLQSLTHI
EECCEEECCCCEECCCCEEEECCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MRKTTRYHIEGPNPLWQMYQTIRFLRVFKNTAIVLIGRYFPSVKAKRWLYRTFLGMNIGE
CCCCEEEEECCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHCCCCCC
YSALALMVTPDVMFPERITIGRNTVIGFNSTILCHEYLVDEYRIGDVIIGDGVLIGANVT
CEEEEEEEECCCCCCCEEECCCCEEEECCCEEEHHHHHHCCCCCCCEEEECCEEEECCEE
ILPGVTIGDGAVIGAGAIVHRDVLPGERVIGSKLQSLTHI
EECCEEECCCCEECCCCEEEECCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]