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The map label for this gene is metQ [H]

Identifier: 229916536

GI number: 229916536

Start: 761257

End: 762090

Strand: Reverse

Name: metQ [H]

Synonym: EAT1b_0807

Alternate gene names: 229916536

Gene position: 762090-761257 (Counterclockwise)

Preceding gene: 229916537

Following gene: 229916535

Centisome position: 25.4

GC content: 49.88

Gene sequence:

>834_bases
ATGAAAACTTGGAAAACAGTACTCGGATTGACGGCATTATCAGCATTCACAGTTCTCGCGGCTTGCGGCGGGGAAGAGGC
AGAAACAGGTTCTGGCGATGACAAAACATTGATTGTCGGTGCGTCAAACGTACCGCACGCTGAGATTTTAGAGCACGTAC
AAGAAGAATATGAAGCAAAAGGTTACAAACTCGAAATCAAGAAGTTCCAAGACTACGTGCTTCCGAACAAGACGCTCGCG
AGCGGGGAGCTCGATGCGAACTACTTCCAACACGAACCATACCTCAAGTCACAAATGGCTGAAAACAAAGACTACAAATT
CGCATCAGCGGGTGGCGTCCACATCGAGCCGATCGGTGTCTACTCACAAAAATACAAATCGCTCGACGAGCTTCCTGAAG
GTGCGGAAATCATCATGAGCTCATCGGTCGCTGACCACGGACGAATCCTTACGATGCTCCAGTCAGAAGGTCTCATCACG
CTTGCTGAAGGCAAGACGGTCGATGCGACGGTCGCTGACATCGTCGACAACCCGAAAGACTTGAAATTCAAGACGGACGT
GGAAGCAGCACTTCTTCCGACGGCTTACAATAACGGAGAAGGCGATGCGGTCCTCATCAACACGAACTATGCGATCGACG
CAGGTCTCAACCCACTTGAAGATGCAATCGCACTCGAAGGGGAAGATTCACCATACGTCAACTTGATCGTCACGCGTGAA
GGGGACGAAGAAGATGAGCGCGTCAAAGCACTCCTCGAAGTATTGACGTCTGAAGAGACACAACAGTGGATCTTAGACGA
ATATAAAGGTGCGGTCGTACCGGTCAAACAATAA

Upstream 100 bases:

>100_bases
GATCATCGTGTTCGTCATCCAATTCATCGGTGACCGACTCGTCACGAAAATTGATAAACGTTCAGCATAATCACACATAT
ACTATTTGGAGGGATTCATT

Downstream 100 bases:

>100_bases
GTCATCTCGACTCAGGAGTCTTCCTCCTGGGTCTTTTTTTGTCTAGTGTTCTTTCATAAAGAGAAGTACACTACATATAT
GAAAGAAAAGGGGGGATTTT

Product: NLPA lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MKTWKTVLGLTALSAFTVLAACGGEEAETGSGDDKTLIVGASNVPHAEILEHVQEEYEAKGYKLEIKKFQDYVLPNKTLA
SGELDANYFQHEPYLKSQMAENKDYKFASAGGVHIEPIGVYSQKYKSLDELPEGAEIIMSSSVADHGRILTMLQSEGLIT
LAEGKTVDATVADIVDNPKDLKFKTDVEAALLPTAYNNGEGDAVLINTNYAIDAGLNPLEDAIALEGEDSPYVNLIVTRE
GDEEDERVKALLEVLTSEETQQWILDEYKGAVVPVKQ

Sequences:

>Translated_277_residues
MKTWKTVLGLTALSAFTVLAACGGEEAETGSGDDKTLIVGASNVPHAEILEHVQEEYEAKGYKLEIKKFQDYVLPNKTLA
SGELDANYFQHEPYLKSQMAENKDYKFASAGGVHIEPIGVYSQKYKSLDELPEGAEIIMSSSVADHGRILTMLQSEGLIT
LAEGKTVDATVADIVDNPKDLKFKTDVEAALLPTAYNNGEGDAVLINTNYAIDAGLNPLEDAIALEGEDSPYVNLIVTRE
GDEEDERVKALLEVLTSEETQQWILDEYKGAVVPVKQ
>Mature_277_residues
MKTWKTVLGLTALSAFTVLAACGGEEAETGSGDDKTLIVGASNVPHAEILEHVQEEYEAKGYKLEIKKFQDYVLPNKTLA
SGELDANYFQHEPYLKSQMAENKDYKFASAGGVHIEPIGVYSQKYKSLDELPEGAEIIMSSSVADHGRILTMLQSEGLIT
LAEGKTVDATVADIVDNPKDLKFKTDVEAALLPTAYNNGEGDAVLINTNYAIDAGLNPLEDAIALEGEDSPYVNLIVTRE
GDEEDERVKALLEVLTSEETQQWILDEYKGAVVPVKQ

Specific function: Part of the ABC transporter complex metNIQ involved in methionine import. Binds the methionine and transfers it to the membrane-bound permease. It has also been shown to be involved in methionine sulfoxide transport (Probable) [H]

COG id: COG1464

COG function: function code P; ABC-type metal ion transport system, periplasmic component/surface antigen

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nlpA lipoprotein family [H]

Homologues:

Organism=Escherichia coli, GI1786396, Length=270, Percent_Identity=38.1481481481481, Blast_Score=172, Evalue=2e-44,
Organism=Escherichia coli, GI1790093, Length=249, Percent_Identity=34.9397590361446, Blast_Score=148, Evalue=5e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004872 [H]

Pfam domain/function: PF03180 Lipoprotein_9 [H]

EC number: NA

Molecular weight: Translated: 30186; Mature: 30186

Theoretical pI: Translated: 4.14; Mature: 4.14

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTWKTVLGLTALSAFTVLAACGGEEAETGSGDDKTLIVGASNVPHAEILEHVQEEYEAK
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCC
GYKLEIKKFQDYVLPNKTLASGELDANYFQHEPYLKSQMAENKDYKFASAGGVHIEPIGV
CEEEEEEHHHHHCCCCCCCCCCCCCCHHHHCCCHHHHHHHCCCCCEEECCCCEEEEEECH
YSQKYKSLDELPEGAEIIMSSSVADHGRILTMLQSEGLITLAEGKTVDATVADIVDNPKD
HHHHHHHHHHCCCCHHEEEECCCCCCCEEEEEEECCCEEEEECCCCCCHHHHHHHCCCCC
LKFKTDVEAALLPTAYNNGEGDAVLINTNYAIDAGLNPLEDAIALEGEDSPYVNLIVTRE
CEEECCCCEEEECCCCCCCCCCEEEEECCEEEECCCCCHHHHEEECCCCCCEEEEEEECC
GDEEDERVKALLEVLTSEETQQWILDEYKGAVVPVKQ
CCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEECCC
>Mature Secondary Structure
MKTWKTVLGLTALSAFTVLAACGGEEAETGSGDDKTLIVGASNVPHAEILEHVQEEYEAK
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCC
GYKLEIKKFQDYVLPNKTLASGELDANYFQHEPYLKSQMAENKDYKFASAGGVHIEPIGV
CEEEEEEHHHHHCCCCCCCCCCCCCCHHHHCCCHHHHHHHCCCCCEEECCCCEEEEEECH
YSQKYKSLDELPEGAEIIMSSSVADHGRILTMLQSEGLITLAEGKTVDATVADIVDNPKD
HHHHHHHHHHCCCCHHEEEECCCCCCCEEEEEEECCCEEEEECCCCCCHHHHHHHCCCCC
LKFKTDVEAALLPTAYNNGEGDAVLINTNYAIDAGLNPLEDAIALEGEDSPYVNLIVTRE
CEEECCCCEEEECCCCCCCCCCEEEEECCEEEECCCCCHHHHEEECCCCCCEEEEEEECC
GDEEDERVKALLEVLTSEETQQWILDEYKGAVVPVKQ
CCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]