Click here to switch to the map view.

The map label for this gene is sufB [H]

Identifier: 229916530

GI number: 229916530

Start: 754657

End: 756054

Strand: Reverse

Name: sufB [H]

Synonym: EAT1b_0801

Alternate gene names: 229916530

Gene position: 756054-754657 (Counterclockwise)

Preceding gene: 229916531

Following gene: 229916529

Centisome position: 25.2

GC content: 49.21

Gene sequence:

>1398_bases
ATGGCAAAGAACATGCCGGAAATTGGCGATTATAAATACGGCTTCCATGATCGGGACGTTTCGATTTTCCGTTCAGGCCG
TGGCTTGACGACAGAAATCGTAGAGACGATCTCACGCATGAAAGAAGAGCCACAATGGATGCTCGACTTCCGTTTGAAGT
CGTTGGATCACTTCAACAAGATGGCGATGCCGACATGGGGCGGAGACCTCTCGGCACTTGATTTCGACGAAATCACGTAC
TATGTCAAACCATCTGAAAAGACAGAACGCTCATGGGATGAGGTACCAGAAGAAATCAAACGTACGTTTGACAAGCTCGG
TATCCCAGAAGCAGAGCAAAAATACTTGGCAGGTGTCTCGGCTCAGTATGAGTCTGAGGTTGTCTACCACAGCATGCAAG
AAGACTTCGAAGAAAAAGGTGTCATCTTCAAAGATACAGACACAGCCCTTAAAGAAGACGAAGAAATCTTCAAAGAGTAC
TTCGGGAAGTTGATTCCGCCGACGGACAACAAGTTTGCGGCCCTCAACTCAGCAGTTTGGTCGGGTGGCTCGTTCATCTA
CGTACCAAAAGGCGTGAAACTCGAGCAACCGCTCCAAGCCTATTTCCGCATCAACTCGGAAAACATGGGTCAGTTCGAGC
GTACGCTTATCATCGTCGACGAAGGCGCATCGGTTCATTACGTCGAAGGATGTACGGCACCGGTCTATACAACGAACTCG
CTTCACTCAGCGGTCGTTGAGATCTTCGTCAACAAAGATGCATACTGCCGTTACACGACAATCCAAAACTGGGCGAACAA
CGTCTACAACTTGGTAACGAAACGTGCAGTCTGTGAAGCGGGTGGATCAATGGAGTGGATCGATGGAAACATCGGCTCGA
AACTCACGATGAAATACCCGGCTGTCATCTTGAAAGGGGAAGGCGCACGTGGGATGACACTCTCAATCGCCCTCGCTGGT
AAAGGACAGCACCAAGACGCTGGCGCGAAAATGATTCACCTCGCACCAAACACGTCGTCGTCTATCGTCTCGAAATCGAT
TTCGAAACAAGGCGGAAAAGTATCGTACCGTGGAATCGTCCACTTCGGCCGTAAGGCGAAGGGCGCACGCTCGAACATCG
AGTGTGACACGCTCATCATGGACAACGAGTCGACGTCAGATACGATTCCGTACAACGAAATCTTGAACGACCAAGTGTCG
CTCGAGCACGAAGCGAAAGTCTCAAAAGTGTCAGAAGAGCAACTCTTCTACCTTATGAGCCGTGGAATTTCGGAAGAAGA
AGCGACAGAAATGATCGTGATGGGCTTCATCGAGCCATTCACAAAAGAATTGCCAATGGAATATGCGGTCGAAATGAACC
GTCTCATCAAGTTCGAGATGGAAGGTTCGATTGGATAA

Upstream 100 bases:

>100_bases
GTATCGAAATTCCCGGCTCGAATCAAGTGTGCCACGCTCGCGTGGAAAGCGCTTGAAAAAGGAGTAGACGAGGACGCGTA
AGGAGAAGGAGGAGACCGAT

Downstream 100 bases:

>100_bases
GGATTATCCGTCCGAATAACGTAAAGCGTGATTGATGTTTCATACATCAATCACGCTTTTTTATTTACGTTCGCAGGCAA
GAGGTTAGGTCGGGTCGAAA

Product: FeS assembly protein SufB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MAKNMPEIGDYKYGFHDRDVSIFRSGRGLTTEIVETISRMKEEPQWMLDFRLKSLDHFNKMAMPTWGGDLSALDFDEITY
YVKPSEKTERSWDEVPEEIKRTFDKLGIPEAEQKYLAGVSAQYESEVVYHSMQEDFEEKGVIFKDTDTALKEDEEIFKEY
FGKLIPPTDNKFAALNSAVWSGGSFIYVPKGVKLEQPLQAYFRINSENMGQFERTLIIVDEGASVHYVEGCTAPVYTTNS
LHSAVVEIFVNKDAYCRYTTIQNWANNVYNLVTKRAVCEAGGSMEWIDGNIGSKLTMKYPAVILKGEGARGMTLSIALAG
KGQHQDAGAKMIHLAPNTSSSIVSKSISKQGGKVSYRGIVHFGRKAKGARSNIECDTLIMDNESTSDTIPYNEILNDQVS
LEHEAKVSKVSEEQLFYLMSRGISEEEATEMIVMGFIEPFTKELPMEYAVEMNRLIKFEMEGSIG

Sequences:

>Translated_465_residues
MAKNMPEIGDYKYGFHDRDVSIFRSGRGLTTEIVETISRMKEEPQWMLDFRLKSLDHFNKMAMPTWGGDLSALDFDEITY
YVKPSEKTERSWDEVPEEIKRTFDKLGIPEAEQKYLAGVSAQYESEVVYHSMQEDFEEKGVIFKDTDTALKEDEEIFKEY
FGKLIPPTDNKFAALNSAVWSGGSFIYVPKGVKLEQPLQAYFRINSENMGQFERTLIIVDEGASVHYVEGCTAPVYTTNS
LHSAVVEIFVNKDAYCRYTTIQNWANNVYNLVTKRAVCEAGGSMEWIDGNIGSKLTMKYPAVILKGEGARGMTLSIALAG
KGQHQDAGAKMIHLAPNTSSSIVSKSISKQGGKVSYRGIVHFGRKAKGARSNIECDTLIMDNESTSDTIPYNEILNDQVS
LEHEAKVSKVSEEQLFYLMSRGISEEEATEMIVMGFIEPFTKELPMEYAVEMNRLIKFEMEGSIG
>Mature_464_residues
AKNMPEIGDYKYGFHDRDVSIFRSGRGLTTEIVETISRMKEEPQWMLDFRLKSLDHFNKMAMPTWGGDLSALDFDEITYY
VKPSEKTERSWDEVPEEIKRTFDKLGIPEAEQKYLAGVSAQYESEVVYHSMQEDFEEKGVIFKDTDTALKEDEEIFKEYF
GKLIPPTDNKFAALNSAVWSGGSFIYVPKGVKLEQPLQAYFRINSENMGQFERTLIIVDEGASVHYVEGCTAPVYTTNSL
HSAVVEIFVNKDAYCRYTTIQNWANNVYNLVTKRAVCEAGGSMEWIDGNIGSKLTMKYPAVILKGEGARGMTLSIALAGK
GQHQDAGAKMIHLAPNTSSSIVSKSISKQGGKVSYRGIVHFGRKAKGARSNIECDTLIMDNESTSDTIPYNEILNDQVSL
EHEAKVSKVSEEQLFYLMSRGISEEEATEMIVMGFIEPFTKELPMEYAVEMNRLIKFEMEGSIG

Specific function: The sufBCD complex acts synergistically with sufE to stimulate the cysteine desulfurase activity of sufS. The sufBCD complex contributes to the assembly or repair of oxygen-labile iron-sulfur clusters under oxidative stress. May facilitate iron uptake fro

COG id: COG0719

COG function: function code O; ABC-type transport system involved in Fe-S cluster assembly, permease component

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0051 (ycf24) family [H]

Homologues:

Organism=Escherichia coli, GI87081955, Length=478, Percent_Identity=40.3765690376569, Blast_Score=363, Evalue=1e-101,
Organism=Escherichia coli, GI1787971, Length=154, Percent_Identity=27.2727272727273, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010231
- InterPro:   IPR000825 [H]

Pfam domain/function: PF01458 UPF0051 [H]

EC number: NA

Molecular weight: Translated: 52396; Mature: 52265

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNMPEIGDYKYGFHDRDVSIFRSGRGLTTEIVETISRMKEEPQWMLDFRLKSLDHFNK
CCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
MAMPTWGGDLSALDFDEITYYVKPSEKTERSWDEVPEEIKRTFDKLGIPEAEQKYLAGVS
HCCCCCCCCCCCEEHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCC
AQYESEVVYHSMQEDFEEKGVIFKDTDTALKEDEEIFKEYFGKLIPPTDNKFAALNSAVW
CHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHC
SGGSFIYVPKGVKLEQPLQAYFRINSENMGQFERTLIIVDEGASVHYVEGCTAPVYTTNS
CCCCEEEECCCCCCCCHHHHHEEECCCCCCCEEEEEEEEECCCCEEEECCCCCCEEECCH
LHSAVVEIFVNKDAYCRYTTIQNWANNVYNLVTKRAVCEAGGSMEWIDGNIGSKLTMKYP
HHHHHHHHEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEECC
AVILKGEGARGMTLSIALAGKGQHQDAGAKMIHLAPNTSSSIVSKSISKQGGKVSYRGIV
EEEEECCCCCCEEEEEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCEEEEEHHE
HFGRKAKGARSNIECDTLIMDNESTSDTIPYNEILNDQVSLEHEAKVSKVSEEQLFYLMS
EECCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
RGISEEEATEMIVMGFIEPFTKELPMEYAVEMNRLIKFEMEGSIG
CCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEEEEEECCCCC
>Mature Secondary Structure 
AKNMPEIGDYKYGFHDRDVSIFRSGRGLTTEIVETISRMKEEPQWMLDFRLKSLDHFNK
CCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
MAMPTWGGDLSALDFDEITYYVKPSEKTERSWDEVPEEIKRTFDKLGIPEAEQKYLAGVS
HCCCCCCCCCCCEEHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCC
AQYESEVVYHSMQEDFEEKGVIFKDTDTALKEDEEIFKEYFGKLIPPTDNKFAALNSAVW
CHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHC
SGGSFIYVPKGVKLEQPLQAYFRINSENMGQFERTLIIVDEGASVHYVEGCTAPVYTTNS
CCCCEEEECCCCCCCCHHHHHEEECCCCCCCEEEEEEEEECCCCEEEECCCCCCEEECCH
LHSAVVEIFVNKDAYCRYTTIQNWANNVYNLVTKRAVCEAGGSMEWIDGNIGSKLTMKYP
HHHHHHHHEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEECC
AVILKGEGARGMTLSIALAGKGQHQDAGAKMIHLAPNTSSSIVSKSISKQGGKVSYRGIV
EEEEECCCCCCEEEEEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCEEEEEHHE
HFGRKAKGARSNIECDTLIMDNESTSDTIPYNEILNDQVSLEHEAKVSKVSEEQLFYLMS
EECCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
RGISEEEATEMIVMGFIEPFTKELPMEYAVEMNRLIKFEMEGSIG
CCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]