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The map label for this gene is nifU [H]

Identifier: 229916531

GI number: 229916531

Start: 756074

End: 756508

Strand: Reverse

Name: nifU [H]

Synonym: EAT1b_0802

Alternate gene names: 229916531

Gene position: 756508-756074 (Counterclockwise)

Preceding gene: 229916532

Following gene: 229916530

Centisome position: 25.22

GC content: 49.2

Gene sequence:

>435_bases
ATGAGTTTTAATAATCTCGACATGTTGTACCGACAGGTCATCATGGATCACTATAAAAACCCAAGAAACCGTGGCGTCAT
CGAAGATGGCGTGACGGTGGACTTGAACAACCCGACGTGTGGGGATTCGCTTCGTCTTCAACTACAGGTCGAAGACAACA
TCGTCAAAGATGCAAAGTTTGAAGGGGAGGGCTGCTCAATCAGTCTTGCCTCGGCATCGATGATGACCCAAATCGTCAAA
GGAAAAACAGTAGAAGAGGCGCTTCAACTCGCCAACATCTTCTCTGAAATGGTTCAAGGCAAAGAGTACGACACGGATAC
GTTTGACCTCGGTGATATCGAGGCGCTCTCAGGCGTATCGAAATTCCCGGCTCGAATCAAGTGTGCCACGCTCGCGTGGA
AAGCGCTTGAAAAAGGAGTAGACGAGGACGCGTAA

Upstream 100 bases:

>100_bases
CAATCATCGACGGCACGGGCGAGCTTCTATTTGTACAACACAAAAGAAGAAGTCGACCGCTTCATCGAGGGATTACGTCA
AACGAAGGAGTATTTCAATT

Downstream 100 bases:

>100_bases
GGAGAAGGAGGAGACCGATATGGCAAAGAACATGCCGGAAATTGGCGATTATAAATACGGCTTCCATGATCGGGACGTTT
CGATTTTCCGTTCAGGCCGT

Product: SUF system FeS assembly protein, NifU family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 144; Mature: 143

Protein sequence:

>144_residues
MSFNNLDMLYRQVIMDHYKNPRNRGVIEDGVTVDLNNPTCGDSLRLQLQVEDNIVKDAKFEGEGCSISLASASMMTQIVK
GKTVEEALQLANIFSEMVQGKEYDTDTFDLGDIEALSGVSKFPARIKCATLAWKALEKGVDEDA

Sequences:

>Translated_144_residues
MSFNNLDMLYRQVIMDHYKNPRNRGVIEDGVTVDLNNPTCGDSLRLQLQVEDNIVKDAKFEGEGCSISLASASMMTQIVK
GKTVEEALQLANIFSEMVQGKEYDTDTFDLGDIEALSGVSKFPARIKCATLAWKALEKGVDEDA
>Mature_143_residues
SFNNLDMLYRQVIMDHYKNPRNRGVIEDGVTVDLNNPTCGDSLRLQLQVEDNIVKDAKFEGEGCSISLASASMMTQIVKG
KTVEEALQLANIFSEMVQGKEYDTDTFDLGDIEALSGVSKFPARIKCATLAWKALEKGVDEDA

Specific function: May be involved in the formation or repair of [Fe-S] clusters present in iron-sulfur proteins (Potential) [H]

COG id: COG0822

COG function: function code C; NifU homolog involved in Fe-S cluster formation

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifU family [H]

Homologues:

Organism=Homo sapiens, GI56699456, Length=89, Percent_Identity=44.9438202247191, Blast_Score=79, Evalue=2e-15,
Organism=Homo sapiens, GI24307953, Length=84, Percent_Identity=45.2380952380952, Blast_Score=74, Evalue=6e-14,
Organism=Escherichia coli, GI1788878, Length=90, Percent_Identity=40, Blast_Score=75, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17543474, Length=89, Percent_Identity=37.0786516853933, Blast_Score=69, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6324800, Length=140, Percent_Identity=30, Blast_Score=75, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6325122, Length=140, Percent_Identity=28.5714285714286, Blast_Score=71, Evalue=7e-14,
Organism=Drosophila melanogaster, GI21355597, Length=89, Percent_Identity=42.6966292134831, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002871
- InterPro:   IPR011341 [H]

Pfam domain/function: PF01592 NifU_N [H]

EC number: NA

Molecular weight: Translated: 15926; Mature: 15795

Theoretical pI: Translated: 4.31; Mature: 4.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFNNLDMLYRQVIMDHYKNPRNRGVIEDGVTVDLNNPTCGDSLRLQLQVEDNIVKDAKF
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCEEEEEEECCCCCCCCEE
EGEGCSISLASASMMTQIVKGKTVEEALQLANIFSEMVQGKEYDTDTFDLGDIEALSGVS
CCCCCEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCHH
KFPARIKCATLAWKALEKGVDEDA
HCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SFNNLDMLYRQVIMDHYKNPRNRGVIEDGVTVDLNNPTCGDSLRLQLQVEDNIVKDAKF
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCEEEEEEECCCCCCCCEE
EGEGCSISLASASMMTQIVKGKTVEEALQLANIFSEMVQGKEYDTDTFDLGDIEALSGVS
CCCCCEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCHH
KFPARIKCATLAWKALEKGVDEDA
HCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]