BacMap

Definition	Exiguobacterium sp. AT1b, complete genome.
Accession	NC_012673
Length	2,999,895

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The map label for this gene is ytbE [H]

Identifier: 229916495

GI number: 229916495

Start: 721165

End: 721998

Strand: Reverse

Name: ytbE [H]

Synonym: EAT1b_0765

Alternate gene names: 229916495

Gene position: 721998-721165 (Counterclockwise)

Preceding gene: 229916496

Following gene: 229916493

Centisome position: 24.07

GC content: 48.8

Gene sequence:

>834_bases
ATGAAACAATCTATAGAGTTGAATCACGGTCAGAAAGTTCTTCCTGCCATCGGACTAGGTGTCTTCAAAGTAGAAGACGG
AGATACGGTCATCGACGCCGTCGTCGAGGCGATTCGTCAAGGATACCGTCATATCGATACGGCAGCTGTTTATGAGAATG
AAGAGGGTGTCGGTGTCGGAATCCGAAAAGGGATGGAAATCGCAAATATTTCTCGGGATGATTTGTTCATCACCTCAAAA
CTTTGGAATGCCGATCAAGGTTATGAATCAGCTATCGCTGCGTATGAAGCTTCACTCGAACGCCTCGGCTTAGAACAATT
GGATTTGTATTTGATTCATTGGCCGGTGGAAGACAAGATGGTCGAGAGCTGGCGTGCCCTCGAAACGTTGTATCGAGAGG
GGCGTGTCGGGGCAGTCGGTGTGAGTAATTTCCAAATCCATCACCTCGAGACATTGGCTGAAAACAGTGACCTCGTTCCA
GCTGTGAACCAGGTTGAACGGCATCCGTTGCTTCGTCAAACCGAGTTAGCATCGTACATGAGCGAGCACGGCATCGTACC
GGTCGCTTGGGCACCACTTATGCAGGGCGGGTTGTTCGAAGAGCAGACTTTAGTAGAGGTCGCGAAAGAAGTCAATCGTT
CCGTCGCCCAGGTGTTGTTACGGTGGCATCTCCAAACGGGATGGGTGATTATTCCGAAATCCATCAAACCACATCGAATC
GCAGAAAACTTCGATGTACATGACTTTGAGTTGACCAATGATCAAATGGAACGAATCGATGCACTCGACCAGCATAAACG
TGTCGGTCCGGACCCAGACGATTTTGATTTCTAA

Upstream 100 bases:

>100_bases
GGGGCGGCTCTTCTTATGCAGTTACTTCAATGTGCCTTATTCCACAAAAGGAGAATCCCCATTATAGTGAAGGAGAATTC
TCACGAAAGAAGGGGTACAA

Downstream 100 bases:

>100_bases
TGCAGTATGTAAAAAAGTCCTTCTCTCGCTCGAGAGAAGGACTTTTGCCATTATTTTTTAATGTCGAACTTTTCGCTAAG
GAAGTTCACGACTTCTTCAG

Product: 2,5-didehydrogluconate reductase

Products: 2-keto-L-gulonate; NADP [C]

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MKQSIELNHGQKVLPAIGLGVFKVEDGDTVIDAVVEAIRQGYRHIDTAAVYENEEGVGVGIRKGMEIANISRDDLFITSK
LWNADQGYESAIAAYEASLERLGLEQLDLYLIHWPVEDKMVESWRALETLYREGRVGAVGVSNFQIHHLETLAENSDLVP
AVNQVERHPLLRQTELASYMSEHGIVPVAWAPLMQGGLFEEQTLVEVAKEVNRSVAQVLLRWHLQTGWVIIPKSIKPHRI
AENFDVHDFELTNDQMERIDALDQHKRVGPDPDDFDF

Sequences:

>Translated_277_residues
MKQSIELNHGQKVLPAIGLGVFKVEDGDTVIDAVVEAIRQGYRHIDTAAVYENEEGVGVGIRKGMEIANISRDDLFITSK
LWNADQGYESAIAAYEASLERLGLEQLDLYLIHWPVEDKMVESWRALETLYREGRVGAVGVSNFQIHHLETLAENSDLVP
AVNQVERHPLLRQTELASYMSEHGIVPVAWAPLMQGGLFEEQTLVEVAKEVNRSVAQVLLRWHLQTGWVIIPKSIKPHRI
AENFDVHDFELTNDQMERIDALDQHKRVGPDPDDFDF
>Mature_277_residues
MKQSIELNHGQKVLPAIGLGVFKVEDGDTVIDAVVEAIRQGYRHIDTAAVYENEEGVGVGIRKGMEIANISRDDLFITSK
LWNADQGYESAIAAYEASLERLGLEQLDLYLIHWPVEDKMVESWRALETLYREGRVGAVGVSNFQIHHLETLAENSDLVP
AVNQVERHPLLRQTELASYMSEHGIVPVAWAPLMQGGLFEEQTLVEVAKEVNRSVAQVLLRWHLQTGWVIIPKSIKPHRI
AENFDVHDFELTNDQMERIDALDQHKRVGPDPDDFDF

Specific function: Reduction [C]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI24497577, Length=298, Percent_Identity=36.5771812080537, Blast_Score=182, Evalue=3e-46,
Organism=Homo sapiens, GI5174391, Length=298, Percent_Identity=36.5771812080537, Blast_Score=182, Evalue=3e-46,
Organism=Homo sapiens, GI223468663, Length=299, Percent_Identity=37.4581939799331, Blast_Score=178, Evalue=4e-45,
Organism=Homo sapiens, GI4502049, Length=300, Percent_Identity=38, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI93277124, Length=306, Percent_Identity=36.2745098039216, Blast_Score=167, Evalue=8e-42,
Organism=Homo sapiens, GI310109922, Length=280, Percent_Identity=35.7142857142857, Blast_Score=164, Evalue=6e-41,
Organism=Homo sapiens, GI5453543, Length=300, Percent_Identity=33.6666666666667, Blast_Score=163, Evalue=1e-40,
Organism=Homo sapiens, GI310109920, Length=300, Percent_Identity=33.6666666666667, Blast_Score=163, Evalue=1e-40,
Organism=Homo sapiens, GI45446745, Length=300, Percent_Identity=34, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI4503285, Length=300, Percent_Identity=34, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI5174695, Length=299, Percent_Identity=33.1103678929766, Blast_Score=159, Evalue=2e-39,
Organism=Homo sapiens, GI24497585, Length=300, Percent_Identity=32.3333333333333, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI291291012, Length=280, Percent_Identity=36.0714285714286, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI24497583, Length=298, Percent_Identity=32.5503355704698, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI300116273, Length=278, Percent_Identity=32.7338129496403, Blast_Score=141, Evalue=6e-34,
Organism=Homo sapiens, GI300116271, Length=277, Percent_Identity=31.7689530685921, Blast_Score=133, Evalue=1e-31,
Organism=Homo sapiens, GI207028673, Length=128, Percent_Identity=39.84375, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI310109926, Length=128, Percent_Identity=39.0625, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI310109924, Length=154, Percent_Identity=33.1168831168831, Blast_Score=82, Evalue=8e-16,
Organism=Escherichia coli, GI87082198, Length=275, Percent_Identity=45.8181818181818, Blast_Score=251, Evalue=4e-68,
Organism=Escherichia coli, GI1786400, Length=267, Percent_Identity=32.9588014981273, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1788081, Length=282, Percent_Identity=30.8510638297872, Blast_Score=97, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71998625, Length=263, Percent_Identity=35.7414448669202, Blast_Score=178, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17537077, Length=299, Percent_Identity=33.7792642140468, Blast_Score=174, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI17537075, Length=308, Percent_Identity=34.0909090909091, Blast_Score=172, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17552492, Length=273, Percent_Identity=35.5311355311355, Blast_Score=170, Evalue=8e-43,
Organism=Caenorhabditis elegans, GI17564128, Length=300, Percent_Identity=33.3333333333333, Blast_Score=166, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17538386, Length=274, Percent_Identity=33.9416058394161, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17561298, Length=276, Percent_Identity=35.1449275362319, Blast_Score=158, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17550248, Length=295, Percent_Identity=33.8983050847458, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17537079, Length=301, Percent_Identity=30.8970099667774, Blast_Score=146, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17561300, Length=255, Percent_Identity=34.1176470588235, Blast_Score=134, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17562292, Length=272, Percent_Identity=29.0441176470588, Blast_Score=131, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17566692, Length=288, Percent_Identity=30.5555555555556, Blast_Score=130, Evalue=8e-31,
Organism=Saccharomyces cerevisiae, GI6324694, Length=294, Percent_Identity=36.734693877551, Blast_Score=185, Evalue=7e-48,
Organism=Saccharomyces cerevisiae, GI6320576, Length=293, Percent_Identity=35.8361774744027, Blast_Score=183, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6322556, Length=278, Percent_Identity=38.1294964028777, Blast_Score=182, Evalue=5e-47,
Organism=Saccharomyces cerevisiae, GI6321896, Length=318, Percent_Identity=33.0188679245283, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6319625, Length=291, Percent_Identity=34.020618556701, Blast_Score=146, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6320079, Length=288, Percent_Identity=30.2083333333333, Blast_Score=124, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24662789, Length=289, Percent_Identity=35.2941176470588, Blast_Score=176, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24657054, Length=285, Percent_Identity=35.7894736842105, Blast_Score=168, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24663317, Length=299, Percent_Identity=32.1070234113712, Blast_Score=165, Evalue=3e-41,
Organism=Drosophila melanogaster, GI24644950, Length=313, Percent_Identity=32.9073482428115, Blast_Score=165, Evalue=3e-41,
Organism=Drosophila melanogaster, GI20129731, Length=295, Percent_Identity=34.2372881355932, Blast_Score=161, Evalue=4e-40,
Organism=Drosophila melanogaster, GI221378297, Length=333, Percent_Identity=30.9309309309309, Blast_Score=157, Evalue=6e-39,
Organism=Drosophila melanogaster, GI21356425, Length=295, Percent_Identity=32.2033898305085, Blast_Score=157, Evalue=8e-39,
Organism=Drosophila melanogaster, GI24662785, Length=287, Percent_Identity=37.2822299651568, Blast_Score=154, Evalue=7e-38,
Organism=Drosophila melanogaster, GI24662781, Length=297, Percent_Identity=36.7003367003367, Blast_Score=153, Evalue=1e-37,
Organism=Drosophila melanogaster, GI45553081, Length=293, Percent_Identity=30.7167235494881, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI281366140, Length=256, Percent_Identity=31.25, Blast_Score=134, Evalue=8e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: NA

Molecular weight: Translated: 31326; Mature: 31326

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00063 ALDOKETO_REDUCTASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQSIELNHGQKVLPAIGLGVFKVEDGDTVIDAVVEAIRQGYRHIDTAAVYENEEGVGVG
CCCCCCCCCCCEEHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEH
IRKGMEIANISRDDLFITSKLWNADQGYESAIAAYEASLERLGLEQLDLYLIHWPVEDKM
HHCCCCHHCCCCCCEEEEHHHCCCCCCHHHHHHHHHHHHHHCCHHHEEEEEEEECCHHHH
VESWRALETLYREGRVGAVGVSNFQIHHLETLAENSDLVPAVNQVERHPLLRQTELASYM
HHHHHHHHHHHHCCCCCEEECCCEEEHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHH
SEHGIVPVAWAPLMQGGLFEEQTLVEVAKEVNRSVAQVLLRWHLQTGWVIIPKSIKPHRI
HHCCCEEEEHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHH
AENFDVHDFELTNDQMERIDALDQHKRVGPDPDDFDF
HCCCCCCEEECCHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKQSIELNHGQKVLPAIGLGVFKVEDGDTVIDAVVEAIRQGYRHIDTAAVYENEEGVGVG
CCCCCCCCCCCEEHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEH
IRKGMEIANISRDDLFITSKLWNADQGYESAIAAYEASLERLGLEQLDLYLIHWPVEDKM
HHCCCCHHCCCCCCEEEEHHHCCCCCCHHHHHHHHHHHHHHCCHHHEEEEEEEECCHHHH
VESWRALETLYREGRVGAVGVSNFQIHHLETLAENSDLVPAVNQVERHPLLRQTELASYM
HHHHHHHHHHHHCCCCCEEECCCEEEHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHH
SEHGIVPVAWAPLMQGGLFEEQTLVEVAKEVNRSVAQVLLRWHLQTGWVIIPKSIKPHRI
HHCCCEEEEHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHH
AENFDVHDFELTNDQMERIDALDQHKRVGPDPDDFDF
HCCCCCCEEECCHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2,5-diketo-D-gluconate; NADPH [C]

Specific reaction: 2,5-diketo-D-gluconate + NADPH = 2-keto-L-gulonate + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]