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The map label for this gene is ptsI [H]

Identifier: 229916494

GI number: 229916494

Start: 719396

End: 721114

Strand: Direct

Name: ptsI [H]

Synonym: EAT1b_0764

Alternate gene names: 229916494

Gene position: 719396-721114 (Clockwise)

Preceding gene: 229916491

Following gene: 229916501

Centisome position: 23.98

GC content: 48.05

Gene sequence:

>1719_bases
ATGTCACACATTAAAGGTATTGGTGCATCTGCAGGGATTGCAGTTGCAAAAGCATTCGTCATGGAGACACCTTCATTCGA
TATCCCATCACACAAGATTGAAGATGTCGCTGCAGAAAAGGCTCGTTTCCAAGATGCAATCGCACAATCTAAAACAGAGC
TTGAAGCCATTCGCGAACATACACTTCGTGAGCTCGGTCCAGACAAAGCCGAGATTTTCTCAGCTCACCTTTTAATCGTT
GAGGACCCAGAAATCGTATCGCAAGTGAACGACAAAATTGAAAGCGAAAACATGAACGCTGCGAAGGCACTTGACGAAGT
TGCCCAAACGATGGTCATGATTTTCGAATCAATGGATAACGAATACATGCGCGAACGTGCGGCTGATGTTCGTGACGTGA
CAAAACGTGTCATGGCTCACATTCTCGGAGTGACATTCGTGACTCCAGCTTCGATTTCAGAAGAAGTCATCATTATCGCA
GAGGACTTGACGCCTTCTGATACGGCACAACTTAACCGTAAATACGTCAAAGGATTCGCGACGAACATCGGTGGACGTAC
GTCTCACTCTGCCATCATGTCACGCTCACTTGAAATTCCGGCCGTCGTCGGAACAAAAGTGGCACTTGAAGAAATCAAAA
ACGGTGACCTCGTCATCATCGACGGAACAGAAGGTGACGTCTTCGTCAACCCTTCAGAAGATCTTGTCAAAGAGTACGAA
AGCAAACGTGCTGATTTTGAAGCGTACAAAGAAGAGTTGAAGCAACTCGTCAACGAAGAGTCGATCACAGCGGACGGTCA
CAAAGTGAAGCTTGCTGCGAACATCGGGACACCGAACGACCTCGAAGGCGTTTTGAAAAACGGTTCAGATGCGATCGGGC
TCTATCGTACGGAGTTCCTATATATGGACTCTGAAACGTTCCCGACCGAAGAAGAGCAATACACAGCATACAAAACGGTT
CTTGAAGGGATGGAAGGCAAGCAAGTCGTCATCCGTACACTCGACATCGGTGGCGACAAAGAGTTGTCATACCTTGACCT
CCCGAAAGAAATGAACCCATTCCTCGGCTATCGTGCCATCCGCCTTTGCCTCGAGGAAACAGATTTGTTCCGCACACAAC
TTCGCGCCCTTCTCCGTGCCTCAGCTCACGGGAACCTCGCCATCATGTTCCCAATGATTGCGACACTCGAAGAGTTCCGC
GCGGCGAAAGCCCTTCTTCTCGAAGAAGAAGCGAAGTTGAAAGAAGAAGGAATCGAAGTCGGACAATATGAGACTGGAAT
GATGGTTGAAATTCCATCAACGGCTGTCATGGCGAAACAATTCGCAAAAGAAGTCGACTTCTTCTCAGTCGGTACAAACG
ACTTGATTCAATACACGATGGCGGCTGACCGGATGAACGAAAAAGTTTCTTACTTGTACCAACCGTTCAACCCTGCCATC
TTGAACCTTCTCCACAACGTCATCACAGAAGCACACAAAGCTGGCAAATGGGTCGGCATGTGTGGAGAGATGGCTGGAGA
AGAACTCGCGATTCCAATCCTTCTCGGTCTTGGTCTCGACGAATTCTCAATGTCCGCTTCAAGCGTCCTTCGTGCGCGTA
GCCTCGTGAAACGCTTGACGAAGTCGGAAACAGAAGCAATCGTCAACGAAGTCCTCAACATGGACACAGCTGAAGAAGTC
GTGAACTTCCTTAGCGAAAAGTTCGACATTAAAAAATAA

Upstream 100 bases:

>100_bases
ACTTTAACTTTCCCCGTTCTCGTTAATTCTTTTTTCTTTTCGGGTATGAAGTAACGAGGCAGGAAAGTACACTATTTCTT
TGAAAAGAGGGACTACGCTT

Downstream 100 bases:

>100_bases
TGGCAAAAGTCCTTCTCTCGAGCGAGAGAAGGACTTTTTTACATACTGCATTAGAAATCAAAATCGTCTGGGTCCGGACC
GACACGTTTATGCTGGTCGA

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 572; Mature: 571

Protein sequence:

>572_residues
MSHIKGIGASAGIAVAKAFVMETPSFDIPSHKIEDVAAEKARFQDAIAQSKTELEAIREHTLRELGPDKAEIFSAHLLIV
EDPEIVSQVNDKIESENMNAAKALDEVAQTMVMIFESMDNEYMRERAADVRDVTKRVMAHILGVTFVTPASISEEVIIIA
EDLTPSDTAQLNRKYVKGFATNIGGRTSHSAIMSRSLEIPAVVGTKVALEEIKNGDLVIIDGTEGDVFVNPSEDLVKEYE
SKRADFEAYKEELKQLVNEESITADGHKVKLAANIGTPNDLEGVLKNGSDAIGLYRTEFLYMDSETFPTEEEQYTAYKTV
LEGMEGKQVVIRTLDIGGDKELSYLDLPKEMNPFLGYRAIRLCLEETDLFRTQLRALLRASAHGNLAIMFPMIATLEEFR
AAKALLLEEEAKLKEEGIEVGQYETGMMVEIPSTAVMAKQFAKEVDFFSVGTNDLIQYTMAADRMNEKVSYLYQPFNPAI
LNLLHNVITEAHKAGKWVGMCGEMAGEELAIPILLGLGLDEFSMSASSVLRARSLVKRLTKSETEAIVNEVLNMDTAEEV
VNFLSEKFDIKK

Sequences:

>Translated_572_residues
MSHIKGIGASAGIAVAKAFVMETPSFDIPSHKIEDVAAEKARFQDAIAQSKTELEAIREHTLRELGPDKAEIFSAHLLIV
EDPEIVSQVNDKIESENMNAAKALDEVAQTMVMIFESMDNEYMRERAADVRDVTKRVMAHILGVTFVTPASISEEVIIIA
EDLTPSDTAQLNRKYVKGFATNIGGRTSHSAIMSRSLEIPAVVGTKVALEEIKNGDLVIIDGTEGDVFVNPSEDLVKEYE
SKRADFEAYKEELKQLVNEESITADGHKVKLAANIGTPNDLEGVLKNGSDAIGLYRTEFLYMDSETFPTEEEQYTAYKTV
LEGMEGKQVVIRTLDIGGDKELSYLDLPKEMNPFLGYRAIRLCLEETDLFRTQLRALLRASAHGNLAIMFPMIATLEEFR
AAKALLLEEEAKLKEEGIEVGQYETGMMVEIPSTAVMAKQFAKEVDFFSVGTNDLIQYTMAADRMNEKVSYLYQPFNPAI
LNLLHNVITEAHKAGKWVGMCGEMAGEELAIPILLGLGLDEFSMSASSVLRARSLVKRLTKSETEAIVNEVLNMDTAEEV
VNFLSEKFDIKK
>Mature_571_residues
SHIKGIGASAGIAVAKAFVMETPSFDIPSHKIEDVAAEKARFQDAIAQSKTELEAIREHTLRELGPDKAEIFSAHLLIVE
DPEIVSQVNDKIESENMNAAKALDEVAQTMVMIFESMDNEYMRERAADVRDVTKRVMAHILGVTFVTPASISEEVIIIAE
DLTPSDTAQLNRKYVKGFATNIGGRTSHSAIMSRSLEIPAVVGTKVALEEIKNGDLVIIDGTEGDVFVNPSEDLVKEYES
KRADFEAYKEELKQLVNEESITADGHKVKLAANIGTPNDLEGVLKNGSDAIGLYRTEFLYMDSETFPTEEEQYTAYKTVL
EGMEGKQVVIRTLDIGGDKELSYLDLPKEMNPFLGYRAIRLCLEETDLFRTQLRALLRASAHGNLAIMFPMIATLEEFRA
AKALLLEEEAKLKEEGIEVGQYETGMMVEIPSTAVMAKQFAKEVDFFSVGTNDLIQYTMAADRMNEKVSYLYQPFNPAIL
NLLHNVITEAHKAGKWVGMCGEMAGEELAIPILLGLGLDEFSMSASSVLRARSLVKRLTKSETEAIVNEVLNMDTAEEVV
NFLSEKFDIKK

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=571, Percent_Identity=45.3590192644483, Blast_Score=514, Evalue=1e-147,
Organism=Escherichia coli, GI48994992, Length=474, Percent_Identity=38.3966244725738, Blast_Score=354, Evalue=8e-99,
Organism=Escherichia coli, GI1788726, Length=563, Percent_Identity=33.9253996447602, Blast_Score=337, Evalue=9e-94,
Organism=Escherichia coli, GI1789193, Length=568, Percent_Identity=32.9225352112676, Blast_Score=298, Evalue=5e-82,
Organism=Escherichia coli, GI1787994, Length=429, Percent_Identity=28.2051282051282, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI226510935, Length=132, Percent_Identity=31.0606060606061, Blast_Score=81, Evalue=2e-16,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 63375; Mature: 63244

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHIKGIGASAGIAVAKAFVMETPSFDIPSHKIEDVAAEKARFQDAIAQSKTELEAIREH
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLRELGPDKAEIFSAHLLIVEDPEIVSQVNDKIESENMNAAKALDEVAQTMVMIFESMDN
HHHHHCCCHHHHHEEEEEEEECHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCH
EYMRERAADVRDVTKRVMAHILGVTFVTPASISEEVIIIAEDLTPSDTAQLNRKYVKGFA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHH
TNIGGRTSHSAIMSRSLEIPAVVGTKVALEEIKNGDLVIIDGTEGDVFVNPSEDLVKEYE
HCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCEEECCCHHHHHHHH
SKRADFEAYKEELKQLVNEESITADGHKVKLAANIGTPNDLEGVLKNGSDAIGLYRTEFL
HHCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCEEEEEEEEE
YMDSETFPTEEEQYTAYKTVLEGMEGKQVVIRTLDIGGDKELSYLDLPKEMNPFLGYRAI
EECCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCEECCCHHCCHHHHHHHH
RLCLEETDLFRTQLRALLRASAHGNLAIMFPMIATLEEFRAAKALLLEEEAKLKEEGIEV
HHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GQYETGMMVEIPSTAVMAKQFAKEVDFFSVGTNDLIQYTMAADRMNEKVSYLYQPFNPAI
CCCCCCEEEECCHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LNLLHNVITEAHKAGKWVGMCGEMAGEELAIPILLGLGLDEFSMSASSVLRARSLVKRLT
HHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEHHHEECCCHHHHHHHHHHHHHHHHHHHHH
KSETEAIVNEVLNMDTAEEVVNFLSEKFDIKK
HHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SHIKGIGASAGIAVAKAFVMETPSFDIPSHKIEDVAAEKARFQDAIAQSKTELEAIREH
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLRELGPDKAEIFSAHLLIVEDPEIVSQVNDKIESENMNAAKALDEVAQTMVMIFESMDN
HHHHHCCCHHHHHEEEEEEEECHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCH
EYMRERAADVRDVTKRVMAHILGVTFVTPASISEEVIIIAEDLTPSDTAQLNRKYVKGFA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHH
TNIGGRTSHSAIMSRSLEIPAVVGTKVALEEIKNGDLVIIDGTEGDVFVNPSEDLVKEYE
HCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCEEECCCHHHHHHHH
SKRADFEAYKEELKQLVNEESITADGHKVKLAANIGTPNDLEGVLKNGSDAIGLYRTEFL
HHCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCEEEEEEEEE
YMDSETFPTEEEQYTAYKTVLEGMEGKQVVIRTLDIGGDKELSYLDLPKEMNPFLGYRAI
EECCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCEECCCHHCCHHHHHHHH
RLCLEETDLFRTQLRALLRASAHGNLAIMFPMIATLEEFRAAKALLLEEEAKLKEEGIEV
HHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GQYETGMMVEIPSTAVMAKQFAKEVDFFSVGTNDLIQYTMAADRMNEKVSYLYQPFNPAI
CCCCCCEEEECCHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LNLLHNVITEAHKAGKWVGMCGEMAGEELAIPILLGLGLDEFSMSASSVLRARSLVKRLT
HHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEHHHEECCCHHHHHHHHHHHHHHHHHHHHH
KSETEAIVNEVLNMDTAEEVVNFLSEKFDIKK
HHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA