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The map label for this gene is yugI [H]

Identifier: 229916479

GI number: 229916479

Start: 703550

End: 703942

Strand: Reverse

Name: yugI [H]

Synonym: EAT1b_0749

Alternate gene names: 229916479

Gene position: 703942-703550 (Counterclockwise)

Preceding gene: 229916480

Following gene: 229916478

Centisome position: 23.47

GC content: 42.49

Gene sequence:

>393_bases
ATGGCAAAATACGAAAAAGATCAAGTATTAACAGGTAAAGTAACAGGAATCCAACCTTTCGGTGCTTTCGTAGCACTCGA
TGACGAGACTCAAGGTCTTGTACACATTTCAGAAATCTCACACGACTACGTAAAAGACGTAAACGATTACGTTCAAGTTG
GTCAAGAAGTTGAAGTGAAAATCCTTGAAGTGGACGAAGCTTCTAAAAAAATCAAGCTTTCAATGAAAGCGACGCAAGAA
GCTCCAAAACGCGAGAAGTCTGCGAAGCCAGCTCGTCGTGCGCCACGCCGCAACGAAGCAGCTTCAAACTTCAAGCAAGA
AGAAGCTCCAGGATTCACAGTCCTCAAAGACAAACTTGAAGACTGGATCAAAAACACAAACTTCAAGAAGTAA

Upstream 100 bases:

>100_bases
TACCAACACGTCAAAAATAATCTTTTTTACTAAATTCTACACTTTATCGAAAAAAGTATGTTATAGTAAGTTGATGAATT
TAGCATAGGAGTTGTTTGTA

Downstream 100 bases:

>100_bases
GTGTTTCAAGGTGGCCCGTTCCAAACGGGTCACCTTTTTTTGTTTTACAGGAATCGGTGGGACGTATGACGAAACTGTAA
ACCGAATAGGAGGAGATCAT

Product: RNA binding S1 domain protein

Products: RNAn; a nucleoside diphosphate [C]

Alternate protein names: GSP13 [H]

Number of amino acids: Translated: 130; Mature: 129

Protein sequence:

>130_residues
MAKYEKDQVLTGKVTGIQPFGAFVALDDETQGLVHISEISHDYVKDVNDYVQVGQEVEVKILEVDEASKKIKLSMKATQE
APKREKSAKPARRAPRRNEAASNFKQEEAPGFTVLKDKLEDWIKNTNFKK

Sequences:

>Translated_130_residues
MAKYEKDQVLTGKVTGIQPFGAFVALDDETQGLVHISEISHDYVKDVNDYVQVGQEVEVKILEVDEASKKIKLSMKATQE
APKREKSAKPARRAPRRNEAASNFKQEEAPGFTVLKDKLEDWIKNTNFKK
>Mature_129_residues
AKYEKDQVLTGKVTGIQPFGAFVALDDETQGLVHISEISHDYVKDVNDYVQVGQEVEVKILEVDEASKKIKLSMKATQEA
PKREKSAKPARRAPRRNEAASNFKQEEAPGFTVLKDKLEDWIKNTNFKK

Specific function: Involved In Mrna Degradation. Hydrolyzes Single-Stranded Polyribonucleotides Processively In The 3' To 5' Direction. Involved In The RNA Degradosome, A Multi-Enzyme Complex Important In RNA Processing And Messenger RNA Degradation. [C]

COG id: COG1098

COG function: function code J; Predicted RNA binding protein (contains ribosomal protein S1 domain)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI145693187, Length=79, Percent_Identity=53.1645569620253, Blast_Score=76, Evalue=7e-16,
Organism=Escherichia coli, GI87082262, Length=109, Percent_Identity=33.9449541284404, Blast_Score=66, Evalue=8e-13,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR000110
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00575 S1 [H]

EC number: 2.7.7.8 [C]

Molecular weight: Translated: 14707; Mature: 14576

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKYEKDQVLTGKVTGIQPFGAFVALDDETQGLVHISEISHDYVKDVNDYVQVGQEVEVK
CCCCCCCCEEECCCCCCCCCCEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCEEE
ILEVDEASKKIKLSMKATQEAPKREKSAKPARRAPRRNEAASNFKQEEAPGFTVLKDKLE
EEEECCCCCEEEEEHHHHHHCHHHHHCCCCHHCCCCCCHHHHHCCHHCCCCHHHHHHHHH
DWIKNTNFKK
HHHHCCCCCC
>Mature Secondary Structure 
AKYEKDQVLTGKVTGIQPFGAFVALDDETQGLVHISEISHDYVKDVNDYVQVGQEVEVK
CCCCCCCEEECCCCCCCCCCEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCEEE
ILEVDEASKKIKLSMKATQEAPKREKSAKPARRAPRRNEAASNFKQEEAPGFTVLKDKLE
EEEECCCCCEEEEEHHHHHHCHHHHHCCCCHHCCCCCCHHHHHCCHHCCCCHHHHHHHHH
DWIKNTNFKK
HHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 4500 [C]

Specific activity: NA

Km value (mM): NA

Substrates: RNAn+1; phosphate [C]

Specific reaction: RNAn+1 + phosphate = RNAn + a nucleoside diphosphate [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 9298659 [H]