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The map label for this gene is yugJ [H]

Identifier: 229916477

GI number: 229916477

Start: 701219

End: 702382

Strand: Reverse

Name: yugJ [H]

Synonym: EAT1b_0747

Alternate gene names: 229916477

Gene position: 702382-701219 (Counterclockwise)

Preceding gene: 229916478

Following gene: 229916476

Centisome position: 23.41

GC content: 51.29

Gene sequence:

>1164_bases
ATGCACAACTTTGAATACAAAAACCCGACAAAACTAATTTTTGGGACACATCAGATCGAAAAATTAGCAGGAGAACTCGA
AGCGCTCGACGCGAAGAAAGTGATGCTCGTCTATGGTGGCGGAAGCATCAAAAAGAACGGGACGTACGATGAAGTCGTGG
CCGAACTTGAAAAAGCAGGCATCTCATACGTAGACTTCTCAGGTGTTGAACCGAACCCACGCATCGAGACGGTACGTCGT
GCGGTGAAATTTGCGCGTGAAGAGGACGTTGACGCCTTGCTTGCTGTCGGTGGCGGTTCCGTCATCGACTGTACAAAATT
GATTTCAGCAGCGATTCCATATGACGGGGACGCGTGGGACATCGTGCTTCGTGAACATATCCCGACAGAGGCGGTACCGT
TCGGGACTGTCCTTACACTCGCTGCGACAGGGTCGGAGATGAACTCGGGATCGGTCATTACGAACTGGGAGACTCAAGAG
AAGTTTGGATGGGGCCACCCGCTCGTGTTCCCGACATTCTCGATCCTCGACCCACGTTACACGGTCTCAGTTCCAAAAGA
CCAGACAATCTATGGCATCGTCGACATGATGAGCCACTGTTTCGAACAATACTTCCACCCGAACCATGCACCGGTACAAG
AACGCATGACAGAAGGTGTCTTGAAAGCAGTCGTTGAATCGGCACCAAAACTCGTGAACGATTTAGAGAACGTAGACCTT
CGTGCCATCATCTTGTTCTCAGGTACGATGGCATTGAACGGTGTCCTGCAAATGGGTGCATCGGGTGACTGGGCATCGCA
CAACATCGAACACGCAGTATCCGCTGTCCATGATATCCCGCATGCCGGAGGTCTCGCGATTCTCTTCCCACGTTGGATGG
AGCATGTATTAGATGAAGACAACGCGGCTCGCTTCGCACGACTCGGTACAGAAGTATTTGATGTCGAGCCTGGCGAGAAC
GATTTGGAAACAGGGAAACGTACGATCCAAGCTGTACGTGCATTCTTCGACCAACTCGGTGCACCGAACTCGCTTCGTGA
CTATGAGATCGATGAGTCGACATTCGATGCGATTCTCGATTCTGCGATGAAACGTGGCGCATTCGGTCGTTTCCGTACGC
TCGAACGGGAAGATGTCGCACATATCCTCGAACTCAGTAAATAA

Upstream 100 bases:

>100_bases
GGATCATCGATCGCATCTACGCATAAGCACGATACTTTTCATTCCGTAAAAACAGGGTATATAGTGTAGGTGTTACATAT
TTCTATGAGGAGGATGACTT

Downstream 100 bases:

>100_bases
GGCGATTTCTTGCCTATTTTGCAATAATGATTGAACGAATCTAACGTTTCCGTTTATGATGGGTCATGTATGGCTCTTTT
ACGAGCGATTTAAAGGAGGA

Product: iron-containing alcohol dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MHNFEYKNPTKLIFGTHQIEKLAGELEALDAKKVMLVYGGGSIKKNGTYDEVVAELEKAGISYVDFSGVEPNPRIETVRR
AVKFAREEDVDALLAVGGGSVIDCTKLISAAIPYDGDAWDIVLREHIPTEAVPFGTVLTLAATGSEMNSGSVITNWETQE
KFGWGHPLVFPTFSILDPRYTVSVPKDQTIYGIVDMMSHCFEQYFHPNHAPVQERMTEGVLKAVVESAPKLVNDLENVDL
RAIILFSGTMALNGVLQMGASGDWASHNIEHAVSAVHDIPHAGGLAILFPRWMEHVLDEDNAARFARLGTEVFDVEPGEN
DLETGKRTIQAVRAFFDQLGAPNSLRDYEIDESTFDAILDSAMKRGAFGRFRTLEREDVAHILELSK

Sequences:

>Translated_387_residues
MHNFEYKNPTKLIFGTHQIEKLAGELEALDAKKVMLVYGGGSIKKNGTYDEVVAELEKAGISYVDFSGVEPNPRIETVRR
AVKFAREEDVDALLAVGGGSVIDCTKLISAAIPYDGDAWDIVLREHIPTEAVPFGTVLTLAATGSEMNSGSVITNWETQE
KFGWGHPLVFPTFSILDPRYTVSVPKDQTIYGIVDMMSHCFEQYFHPNHAPVQERMTEGVLKAVVESAPKLVNDLENVDL
RAIILFSGTMALNGVLQMGASGDWASHNIEHAVSAVHDIPHAGGLAILFPRWMEHVLDEDNAARFARLGTEVFDVEPGEN
DLETGKRTIQAVRAFFDQLGAPNSLRDYEIDESTFDAILDSAMKRGAFGRFRTLEREDVAHILELSK
>Mature_387_residues
MHNFEYKNPTKLIFGTHQIEKLAGELEALDAKKVMLVYGGGSIKKNGTYDEVVAELEKAGISYVDFSGVEPNPRIETVRR
AVKFAREEDVDALLAVGGGSVIDCTKLISAAIPYDGDAWDIVLREHIPTEAVPFGTVLTLAATGSEMNSGSVITNWETQE
KFGWGHPLVFPTFSILDPRYTVSVPKDQTIYGIVDMMSHCFEQYFHPNHAPVQERMTEGVLKAVVESAPKLVNDLENVDL
RAIILFSGTMALNGVLQMGASGDWASHNIEHAVSAVHDIPHAGGLAILFPRWMEHVLDEDNAARFARLGTEVFDVEPGEN
DLETGKRTIQAVRAFFDQLGAPNSLRDYEIDESTFDAILDSAMKRGAFGRFRTLEREDVAHILELSK

Specific function: Unknown

COG id: COG1979

COG function: function code C; Uncharacterized oxidoreductases, Fe-dependent alcohol dehydrogenase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1789386, Length=372, Percent_Identity=40.5913978494624, Blast_Score=258, Evalue=4e-70,
Organism=Escherichia coli, GI48994951, Length=330, Percent_Identity=26.3636363636364, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI87082107, Length=300, Percent_Identity=27, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1789163, Length=374, Percent_Identity=23.7967914438503, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787493, Length=361, Percent_Identity=21.0526315789474, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17537053, Length=204, Percent_Identity=27.4509803921569, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6321181, Length=350, Percent_Identity=24, Blast_Score=97, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24657991, Length=419, Percent_Identity=23.1503579952267, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: 1.1.-.- [C]

Molecular weight: Translated: 42720; Mature: 42720

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00913 ADH_IRON_1 ; PS00060 ADH_IRON_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNFEYKNPTKLIFGTHQIEKLAGELEALDAKKVMLVYGGGSIKKNGTYDEVVAELEKAG
CCCCCCCCCCEEEECHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCC
ISYVDFSGVEPNPRIETVRRAVKFAREEDVDALLAVGGGSVIDCTKLISAAIPYDGDAWD
CCEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCCHHE
IVLREHIPTEAVPFGTVLTLAATGSEMNSGSVITNWETQEKFGWGHPLVFPTFSILDPRY
EEEHHCCCCCCCCCCHHEEEEECCCCCCCCCEEECCCCHHHCCCCCCHHHCHHHHCCCCE
TVSVPKDQTIYGIVDMMSHCFEQYFHPNHAPVQERMTEGVLKAVVESAPKLVNDLENVDL
EEECCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
RAIILFSGTMALNGVLQMGASGDWASHNIEHAVSAVHDIPHAGGLAILFPRWMEHVLDED
EEEEEEECHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHHCCC
NAARFARLGTEVFDVEPGENDLETGKRTIQAVRAFFDQLGAPNSLRDYEIDESTFDAILD
CHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
SAMKRGAFGRFRTLEREDVAHILELSK
HHHHCCCCHHHHCCCHHHHHHHHHCCC
>Mature Secondary Structure
MHNFEYKNPTKLIFGTHQIEKLAGELEALDAKKVMLVYGGGSIKKNGTYDEVVAELEKAG
CCCCCCCCCCEEEECHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCC
ISYVDFSGVEPNPRIETVRRAVKFAREEDVDALLAVGGGSVIDCTKLISAAIPYDGDAWD
CCEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCCHHE
IVLREHIPTEAVPFGTVLTLAATGSEMNSGSVITNWETQEKFGWGHPLVFPTFSILDPRY
EEEHHCCCCCCCCCCHHEEEEECCCCCCCCCEEECCCCHHHCCCCCCHHHCHHHHCCCCE
TVSVPKDQTIYGIVDMMSHCFEQYFHPNHAPVQERMTEGVLKAVVESAPKLVNDLENVDL
EEECCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
RAIILFSGTMALNGVLQMGASGDWASHNIEHAVSAVHDIPHAGGLAILFPRWMEHVLDED
EEEEEEECHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHHCCC
NAARFARLGTEVFDVEPGENDLETGKRTIQAVRAFFDQLGAPNSLRDYEIDESTFDAILD
CHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
SAMKRGAFGRFRTLEREDVAHILELSK
HHHHCCCCHHHHCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377 [H]