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The map label for this gene is ytzG [H]

Identifier: 229916458

GI number: 229916458

Start: 679737

End: 680450

Strand: Reverse

Name: ytzG [H]

Synonym: EAT1b_0728

Alternate gene names: 229916458

Gene position: 680450-679737 (Counterclockwise)

Preceding gene: 229916459

Following gene: 229916455

Centisome position: 22.68

GC content: 50.98

Gene sequence:

>714_bases
ATGCGGATTGATAAGTTGTTAGCGAACATGGGATATGGTTCACGAAAAGACGTGAAAATCCTATTGAAACAAGGGGCGGT
GCGAGTCGATGAGGCACTTGTAAAAGATGCGAAACGGCACGTCGACCCATCATCAGAGAAAGTCACGGTGTTCGGGGAAG
TGGTCGAGTATCGTCCGTACATCTATTTCATGATGAATAAGCCGGAAGGGGTCATCAGTGCGACGGAAGATCAAGTCGAG
ACGACAGTCATCGAGTTGATTGATTCTGAATATGCCCATTATGACCTCTTCCCGGTCGGACGGCTTGATAAAGATACGAC
GGGACTGCTCCTCATCACGAACGACGGCGCGTTCAATCATGCGCTCATGTCACCTCGCAAGCACGTCGACAAGGTGTATA
TCGCCGAAGTCGACGGGGAGATGACAGTAGAGGATATCAAGACGTTCGCGAATGGGGTCGAGTTAGAGGATGGGTATACG
ACAAAGCCGGCTCGTCTTGAGATACTGAGCCGGAGCGGACGTCGGTCGGTCATTCGTCTCACGTTGTCTGAGGGGAAATA
TCACCAAGTGAAACGAATGGTCGCGGCGGTCGGCAAACATGTCGAACGATTGGAGCGTGTTCAAATCGGGGAGCTAGAAC
TCGATGAGACGCTTGAGCTCGGGGCATATCGTGAGTTGACGGAAGAAGAGGTTCAACTGTTTCTTCAATCATGA

Upstream 100 bases:

>100_bases
GAGTGACAATCGGCGTTCTCGTATATGGATACCTCGGCTGGCGGAGCCATCTCGCAGGTAAATTACTAGGGAAACGCTTT
GAATATCGGAGGAAAATGTA

Downstream 100 bases:

>100_bases
GTAAGATGAGAGAATCTGTCTGATGGGACGGATTCTCTTTTTGTATAAAAAGCTCTGTTTTTCGATTATATCTTTTTGAA
AATGGCATCAAAATCATCAC

Product: pseudouridine synthase

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MRIDKLLANMGYGSRKDVKILLKQGAVRVDEALVKDAKRHVDPSSEKVTVFGEVVEYRPYIYFMMNKPEGVISATEDQVE
TTVIELIDSEYAHYDLFPVGRLDKDTTGLLLITNDGAFNHALMSPRKHVDKVYIAEVDGEMTVEDIKTFANGVELEDGYT
TKPARLEILSRSGRRSVIRLTLSEGKYHQVKRMVAAVGKHVERLERVQIGELELDETLELGAYRELTEEEVQLFLQS

Sequences:

>Translated_237_residues
MRIDKLLANMGYGSRKDVKILLKQGAVRVDEALVKDAKRHVDPSSEKVTVFGEVVEYRPYIYFMMNKPEGVISATEDQVE
TTVIELIDSEYAHYDLFPVGRLDKDTTGLLLITNDGAFNHALMSPRKHVDKVYIAEVDGEMTVEDIKTFANGVELEDGYT
TKPARLEILSRSGRRSVIRLTLSEGKYHQVKRMVAAVGKHVERLERVQIGELELDETLELGAYRELTEEEVQLFLQS
>Mature_237_residues
MRIDKLLANMGYGSRKDVKILLKQGAVRVDEALVKDAKRHVDPSSEKVTVFGEVVEYRPYIYFMMNKPEGVISATEDQVE
TTVIELIDSEYAHYDLFPVGRLDKDTTGLLLITNDGAFNHALMSPRKHVDKVYIAEVDGEMTVEDIKTFANGVELEDGYT
TKPARLEILSRSGRRSVIRLTLSEGKYHQVKRMVAAVGKHVERLERVQIGELELDETLELGAYRELTEEEVQLFLQS

Specific function: Responsible For Synthesis Of Pseudouridine From Uracil-516 In 16s Ribosomal RNA. [C]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=232, Percent_Identity=39.6551724137931, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1787524, Length=234, Percent_Identity=31.1965811965812, Blast_Score=103, Evalue=7e-24,
Organism=Escherichia coli, GI1790453, Length=240, Percent_Identity=30, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI87081838, Length=189, Percent_Identity=33.8624338624339, Blast_Score=91, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 26891; Mature: 26891

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIDKLLANMGYGSRKDVKILLKQGAVRVDEALVKDAKRHVDPSSEKVTVFGEVVEYRPY
CCHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHCCCE
IYFMMNKPEGVISATEDQVETTVIELIDSEYAHYDLFPVGRLDKDTTGLLLITNDGAFNH
EEEEEECCCCCEECCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEECCCCCCH
ALMSPRKHVDKVYIAEVDGEMTVEDIKTFANGVELEDGYTTKPARLEILSRSGRRSVIRL
HHCCCHHHCCEEEEEECCCCEEHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCEEEEE
TLSEGKYHQVKRMVAAVGKHVERLERVQIGELELDETLELGAYRELTEEEVQLFLQS
EECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRIDKLLANMGYGSRKDVKILLKQGAVRVDEALVKDAKRHVDPSSEKVTVFGEVVEYRPY
CCHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHCCCE
IYFMMNKPEGVISATEDQVETTVIELIDSEYAHYDLFPVGRLDKDTTGLLLITNDGAFNH
EEEEEECCCCCEECCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEECCCCCCH
ALMSPRKHVDKVYIAEVDGEMTVEDIKTFANGVELEDGYTTKPARLEILSRSGRRSVIRL
HHCCCHHHCCEEEEEECCCCEEHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCEEEEE
TLSEGKYHQVKRMVAAVGKHVERLERVQIGELELDETLELGAYRELTEEEVQLFLQS
EECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]