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The map label for this gene is tyrS1 [H]

Identifier: 229916423

GI number: 229916423

Start: 646815

End: 648062

Strand: Reverse

Name: tyrS1 [H]

Synonym: EAT1b_0693

Alternate gene names: 229916423

Gene position: 648062-646815 (Counterclockwise)

Preceding gene: 229916425

Following gene: 229916422

Centisome position: 21.6

GC content: 49.6

Gene sequence:

>1248_bases
ATGGAACTATTAAAAGACTTAGAGTTTCGAGGGCTTGTCAACCAAATGACAGATGAAGAAGGATTGAAAGAACAACTTAA
ACAACCGACGACGTTATACACGGGATTTGACCCGACAGCGGACTCGCTTCATATCGGGCACTTGCTCCCGATTTTGACGC
TAAAACGTTTTCAACAAGCAGGGCATCAGGTCATCGCGCTCGTCGGTGGGGCGACAGGGATGATCGGAGACCCATCTGGT
CGTTCGGATGAACGTTCATTGAACACGTTGGATACGGTCAAGCTCTTCTCCGATCGAATCAAAGATCAGTTGAGCCGATT
CCTTCCACTTGAAGGGGAGAACCCGATCACGATTCGCAACAACTACGAATGGACGGAAGAGATGTCGATCATCGACTTCC
TTCGCGACATCGGGAAGCACTTCCCAATCAGCTACATGCTTGCGAAAGATTCTGTCGATTCACGCATGGAGAATGGGATC
TCGTACACAGAATTCTCATACATGTTACTTCAATCTTACGACTTCTTGAAACTATACGAGACAGAGAAATGTCGTCTTCA
AGTCGGTGGAAGTGACCAATGGGGGAACATCACGGCAGGTCTTGAATTGATTCGTCGCTTCGGTCATTCAGAAAAAGCGT
TCGGACTTACGGTTCCGCTCGTCACAAAGTCGGACGGACAGAAGTTCGGGAAGACAGCGGGCGGTGCGGTTTGGTTAGAC
GCAGATAAGACGTCACCATACGAGTTCTTCCAGTTCTGGATCAACGTCGACGATGCGGATGTCATCAAGTTCATCAAATA
CTTCACGTTCATGTCGCACGAAGAGATTGAAGCACTTGCGACTGAAGTGACGGAAGCACCTGAGAAACGTGTGGCGCAGC
GCCGACTCGCCGAAGAAATGACAAAGCTCGTCCATGGTGAAGCAGGACTTGAACAAGCGGAACGCATCACGAAAGCGTTC
TTTAGCGGAGATTTGACGTCGCTTCAACCGGAAGACATGGAAGATGCGTTCAAAGGAATGCCGCAGTTCGAGCTTGACGG
TGAGCGCAACATCGTAGACCTCCTCGTCGAGGCGAACATCTCACCATCGAAACGTCAAGCACGTGAAGACGTGACGAACG
GCGCGATTTACTTGAACGGCGAACGCGAACAAGCACTTGATAAAGTCGTCACTCGCGAAGACACGCTCGGTGAATTCACC
ATCATTCGCCGCGGTAAGAAAAAATATTTCGTGATTCGTCACGTATGA

Upstream 100 bases:

>100_bases
GCGGTCGCAAATTGCGACAAGTAGGGTGGTACCGCGGAATCTCCGTCCCTTCTCATATGAGAAGGGACGTTTTTTTATGT
CAATTGAAGGAGTGGAACAG

Downstream 100 bases:

>100_bases
ACATCCTGTCCGTTCCTCATTTGTTGAGGAATGGACTTTTTTTATACATGACGTGCGGTTCGGATTTGCTAAAATGGGGA
GAAAGGAGGGATGACGATGG

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase 1; TyrRS 1 [H]

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MELLKDLEFRGLVNQMTDEEGLKEQLKQPTTLYTGFDPTADSLHIGHLLPILTLKRFQQAGHQVIALVGGATGMIGDPSG
RSDERSLNTLDTVKLFSDRIKDQLSRFLPLEGENPITIRNNYEWTEEMSIIDFLRDIGKHFPISYMLAKDSVDSRMENGI
SYTEFSYMLLQSYDFLKLYETEKCRLQVGGSDQWGNITAGLELIRRFGHSEKAFGLTVPLVTKSDGQKFGKTAGGAVWLD
ADKTSPYEFFQFWINVDDADVIKFIKYFTFMSHEEIEALATEVTEAPEKRVAQRRLAEEMTKLVHGEAGLEQAERITKAF
FSGDLTSLQPEDMEDAFKGMPQFELDGERNIVDLLVEANISPSKRQAREDVTNGAIYLNGEREQALDKVVTREDTLGEFT
IIRRGKKKYFVIRHV

Sequences:

>Translated_415_residues
MELLKDLEFRGLVNQMTDEEGLKEQLKQPTTLYTGFDPTADSLHIGHLLPILTLKRFQQAGHQVIALVGGATGMIGDPSG
RSDERSLNTLDTVKLFSDRIKDQLSRFLPLEGENPITIRNNYEWTEEMSIIDFLRDIGKHFPISYMLAKDSVDSRMENGI
SYTEFSYMLLQSYDFLKLYETEKCRLQVGGSDQWGNITAGLELIRRFGHSEKAFGLTVPLVTKSDGQKFGKTAGGAVWLD
ADKTSPYEFFQFWINVDDADVIKFIKYFTFMSHEEIEALATEVTEAPEKRVAQRRLAEEMTKLVHGEAGLEQAERITKAF
FSGDLTSLQPEDMEDAFKGMPQFELDGERNIVDLLVEANISPSKRQAREDVTNGAIYLNGEREQALDKVVTREDTLGEFT
IIRRGKKKYFVIRHV
>Mature_415_residues
MELLKDLEFRGLVNQMTDEEGLKEQLKQPTTLYTGFDPTADSLHIGHLLPILTLKRFQQAGHQVIALVGGATGMIGDPSG
RSDERSLNTLDTVKLFSDRIKDQLSRFLPLEGENPITIRNNYEWTEEMSIIDFLRDIGKHFPISYMLAKDSVDSRMENGI
SYTEFSYMLLQSYDFLKLYETEKCRLQVGGSDQWGNITAGLELIRRFGHSEKAFGLTVPLVTKSDGQKFGKTAGGAVWLD
ADKTSPYEFFQFWINVDDADVIKFIKYFTFMSHEEIEALATEVTEAPEKRVAQRRLAEEMTKLVHGEAGLEQAERITKAF
FSGDLTSLQPEDMEDAFKGMPQFELDGERNIVDLLVEANISPSKRQAREDVTNGAIYLNGEREQALDKVVTREDTLGEFT
IIRRGKKKYFVIRHV

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=437, Percent_Identity=37.070938215103, Blast_Score=276, Evalue=2e-74,
Organism=Escherichia coli, GI1787925, Length=417, Percent_Identity=53.9568345323741, Blast_Score=447, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI25145802, Length=402, Percent_Identity=35.5721393034826, Blast_Score=241, Evalue=7e-64,
Organism=Caenorhabditis elegans, GI25145805, Length=328, Percent_Identity=32.0121951219512, Blast_Score=176, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6325160, Length=436, Percent_Identity=36.2385321100917, Blast_Score=281, Evalue=2e-76,
Organism=Drosophila melanogaster, GI20130383, Length=407, Percent_Identity=42.014742014742, Blast_Score=323, Evalue=1e-88,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 47181; Mature: 47181

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELLKDLEFRGLVNQMTDEEGLKEQLKQPTTLYTGFDPTADSLHIGHLLPILTLKRFQQA
CCHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEECCCCCCCCEEHHHHHHHHHHHHHHHC
GHQVIALVGGATGMIGDPSGRSDERSLNTLDTVKLFSDRIKDQLSRFLPLEGENPITIRN
CCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECC
NYEWTEEMSIIDFLRDIGKHFPISYMLAKDSVDSRMENGISYTEFSYMLLQSYDFLKLYE
CCCHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHCCCCHHHHHHHHHHCCCHHEEEC
TEKCRLQVGGSDQWGNITAGLELIRRFGHSEKAFGLTVPLVTKSDGQKFGKTAGGAVWLD
CCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHHHCCCCCCEEEEE
ADKTSPYEFFQFWINVDDADVIKFIKYFTFMSHEEIEALATEVTEAPEKRVAQRRLAEEM
CCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHCHHHHHHHHHHHHHH
TKLVHGEAGLEQAERITKAFFSGDLTSLQPEDMEDAFKGMPQFELDGERNIVDLLVEANI
HHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCEECCCCCCCEEEEEECCC
SPSKRQAREDVTNGAIYLNGEREQALDKVVTREDTLGEFTIIRRGKKKYFVIRHV
CCHHHHHHHHHCCCEEEECCCHHHHHHHHHHCCCCCCCCCEEECCCCEEEEEECC
>Mature Secondary Structure
MELLKDLEFRGLVNQMTDEEGLKEQLKQPTTLYTGFDPTADSLHIGHLLPILTLKRFQQA
CCHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEECCCCCCCCEEHHHHHHHHHHHHHHHC
GHQVIALVGGATGMIGDPSGRSDERSLNTLDTVKLFSDRIKDQLSRFLPLEGENPITIRN
CCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECC
NYEWTEEMSIIDFLRDIGKHFPISYMLAKDSVDSRMENGISYTEFSYMLLQSYDFLKLYE
CCCHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHCCCCHHHHHHHHHHCCCHHEEEC
TEKCRLQVGGSDQWGNITAGLELIRRFGHSEKAFGLTVPLVTKSDGQKFGKTAGGAVWLD
CCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHHHCCCCCCEEEEE
ADKTSPYEFFQFWINVDDADVIKFIKYFTFMSHEEIEALATEVTEAPEKRVAQRRLAEEM
CCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHCHHHHHHHHHHHHHH
TKLVHGEAGLEQAERITKAFFSGDLTSLQPEDMEDAFKGMPQFELDGERNIVDLLVEANI
HHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCEECCCCCCCEEEEEECCC
SPSKRQAREDVTNGAIYLNGEREQALDKVVTREDTLGEFTIIRRGKKKYFVIRHV
CCHHHHHHHHHCCCEEEECCCHHHHHHHHHHCCCCCCCCCEEECCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]