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The map label for this gene is ppnK [H]

Identifier: 229916417

GI number: 229916417

Start: 640279

End: 641088

Strand: Direct

Name: ppnK [H]

Synonym: EAT1b_0687

Alternate gene names: 229916417

Gene position: 640279-641088 (Clockwise)

Preceding gene: 229916412

Following gene: 229916420

Centisome position: 21.34

GC content: 48.89

Gene sequence:

>810_bases
ATGAGAAACAACGTCTATTTGTACCATCGTAACGTCAACGAATTCACAAAAGAAGTGAAGAAACTGAGAGAGATTGGGGA
AAAACACGGGTTCAACATCGTGAATCAACCGGAAGACGCGAACATCATCGCTGCAGTAGGCGGGGACGGGGCATTCTTAC
AAGCGGTTCGCTACACAGGCTTTCGTCAAGACGCCATCTACGTCGGCTTCGGTCGTGGATTGAACGAGTTCTATTGTGAT
TTCGACATACATAAACTCGATGAAGTCGATCGCCTCTTCTCGGACAACTCGACGCGAATCGAAGAAGGTCTTGAAGTCCG
GAAGTATCCACTTCTTTCAGCATCGATCAACGAATCGACACCGCTCCTCTGTTTAAATGAGGCTTCGATTAAATCAAGTA
TTATCAAATCACTCGCGATCGAGGTCTATATCGACGACCTACATTTCGAAACATTCCGTGGGGACGGAATGGTTGTATCG
ACACCAACGGGATCGACCGCCTACAACAAATCGCTCAGCGGCGCGGTCGTCGATCCGCTCATCCCATGTATGCAAGTGAG
TGAGATCGCTTCGGTCAACAACAACCGTTACCGTACACTTGGCTCATCCTTCATCGTCCACGAATCGCGCAAGTTGTCAC
TCCGCATCGTCGAAGACGGCAACGACTATCCGATTATCGGGATGGATAACGAGGCGCTCAGCTTGAAGTACACAGACCGA
ATCGATATCGAACTCTCAGACCAGGTCATCAAGACCGTCAAACTTCGGACCAACTCGTTCTGGCATAAAGTGCAACGTTC
ATTCTTCTGA

Upstream 100 bases:

>100_bases
ACAAACTTCCTGCTTTTGGCTCTGTTTCAGTTTTCCCTATATCGGTAGGAAGAAACACGTTATAATAAAGGGGACACCAT
TGACAAAGGGGATGACTCAG

Downstream 100 bases:

>100_bases
TGAAGAAAAGGAGTGGCTTCATCCCTTTCGGGTGAAACCACTCCTCTTTTTACATCGTGGCTTTTTCTGCCTCGTACACG
GGCTTCCCTTCGACGACAGT

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase 2 [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MRNNVYLYHRNVNEFTKEVKKLREIGEKHGFNIVNQPEDANIIAAVGGDGAFLQAVRYTGFRQDAIYVGFGRGLNEFYCD
FDIHKLDEVDRLFSDNSTRIEEGLEVRKYPLLSASINESTPLLCLNEASIKSSIIKSLAIEVYIDDLHFETFRGDGMVVS
TPTGSTAYNKSLSGAVVDPLIPCMQVSEIASVNNNRYRTLGSSFIVHESRKLSLRIVEDGNDYPIIGMDNEALSLKYTDR
IDIELSDQVIKTVKLRTNSFWHKVQRSFF

Sequences:

>Translated_269_residues
MRNNVYLYHRNVNEFTKEVKKLREIGEKHGFNIVNQPEDANIIAAVGGDGAFLQAVRYTGFRQDAIYVGFGRGLNEFYCD
FDIHKLDEVDRLFSDNSTRIEEGLEVRKYPLLSASINESTPLLCLNEASIKSSIIKSLAIEVYIDDLHFETFRGDGMVVS
TPTGSTAYNKSLSGAVVDPLIPCMQVSEIASVNNNRYRTLGSSFIVHESRKLSLRIVEDGNDYPIIGMDNEALSLKYTDR
IDIELSDQVIKTVKLRTNSFWHKVQRSFF
>Mature_269_residues
MRNNVYLYHRNVNEFTKEVKKLREIGEKHGFNIVNQPEDANIIAAVGGDGAFLQAVRYTGFRQDAIYVGFGRGLNEFYCD
FDIHKLDEVDRLFSDNSTRIEEGLEVRKYPLLSASINESTPLLCLNEASIKSSIIKSLAIEVYIDDLHFETFRGDGMVVS
TPTGSTAYNKSLSGAVVDPLIPCMQVSEIASVNNNRYRTLGSSFIVHESRKLSLRIVEDGNDYPIIGMDNEALSLKYTDR
IDIELSDQVIKTVKLRTNSFWHKVQRSFF

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus [H]

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family [H]

Homologues:

Organism=Escherichia coli, GI1788968, Length=231, Percent_Identity=26.4069264069264, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504 [H]

Pfam domain/function: PF01513 NAD_kinase [H]

EC number: =2.7.1.23 [H]

Molecular weight: Translated: 30478; Mature: 30478

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNNVYLYHRNVNEFTKEVKKLREIGEKHGFNIVNQPEDANIIAAVGGDGAFLQAVRYTG
CCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHCC
FRQDAIYVGFGRGLNEFYCDFDIHKLDEVDRLFSDNSTRIEEGLEVRKYPLLSASINEST
CCCCEEEEEECCCCCCEEECCCCHHHHHHHHHHCCCCHHHHCCCHHHCCEEEECCCCCCC
PLLCLNEASIKSSIIKSLAIEVYIDDLHFETFRGDGMVVSTPTGSTAYNKSLSGAVVDPL
CEEEECCHHHHHHHHHHHEEEEEEECEEEEEECCCCEEEECCCCCCCCCCCCCCCHHHHH
IPCMQVSEIASVNNNRYRTLGSSFIVHESRKLSLRIVEDGNDYPIIGMDNEALSLKYTDR
HHHHHHHHHHHCCCCEEEECCCEEEEECCCEEEEEEEECCCCEEEEECCCCEEEEEECCE
IDIELSDQVIKTVKLRTNSFWHKVQRSFF
EEEEEHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRNNVYLYHRNVNEFTKEVKKLREIGEKHGFNIVNQPEDANIIAAVGGDGAFLQAVRYTG
CCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHCC
FRQDAIYVGFGRGLNEFYCDFDIHKLDEVDRLFSDNSTRIEEGLEVRKYPLLSASINEST
CCCCEEEEEECCCCCCEEECCCCHHHHHHHHHHCCCCHHHHCCCHHHCCEEEECCCCCCC
PLLCLNEASIKSSIIKSLAIEVYIDDLHFETFRGDGMVVSTPTGSTAYNKSLSGAVVDPL
CEEEECCHHHHHHHHHHHEEEEEEECEEEEEECCCCEEEECCCCCCCCCCCCCCCHHHHH
IPCMQVSEIASVNNNRYRTLGSSFIVHESRKLSLRIVEDGNDYPIIGMDNEALSLKYTDR
HHHHHHHHHHHCCCCEEEECCCEEEEECCCEEEEEEEECCCCEEEEECCCCEEEEEECCE
IDIELSDQVIKTVKLRTNSFWHKVQRSFF
EEEEEHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]