Click here to switch to the map view.

The map label for this gene is pfkA [H]

Identifier: 229916400

GI number: 229916400

Start: 623098

End: 624066

Strand: Reverse

Name: pfkA [H]

Synonym: EAT1b_0670

Alternate gene names: 229916400

Gene position: 624066-623098 (Counterclockwise)

Preceding gene: 229916401

Following gene: 229916399

Centisome position: 20.8

GC content: 49.54

Gene sequence:

>969_bases
GTGAATTTAAAACGTATTGCGGTATTAACAAGCGGAGGAGACGCTCCTGGAATGAACGCTGCTGTCCGTGCCGTGACCCG
AAAAGCAATTTTTGAAGGTCTTGAAGTGTACGGCGTGTATAACGGCTATCAAGGATTAATCGAAGGTGATTTAGTCGAAT
TAAATCTTGGATCAGTCGGCGATATCATTCAACGTGGAGGTACGTTCCTTCGCTCTGCGCGCTGTCCTGAATTCAGAACA
GAAGAAGGTCGCGCGAAAGCAGTAGAACAGTTGAAGAAGTTCAATATCGATGCCCTCGTGGTCATTGGTGGAGATGGCTC
TTATCGCGGAGCTCAAAAATTGACTGAACTCGGATTCCCAACAATCGGCCTTCCGGGTACAATCGATAACGATATCCCAG
GAACAGATGCGACAATCGGTTTTGACACGGCGCTCAACACAGCGCTTGATGCGATCGATAAGATTCGTGATACGGCTTCA
TCACATGAACGCACGTATGTCATCGAGGTCATGGGGCGCGACGCTGGTGATATCGCGCTTTATTCTGGTCTCGCTGGTGG
TGCTGAGTCGATTCTCGTCCCAGAACGTCCAGAAGATTTGAATGCAATCGTGGAACGAATCAATCACGGGATTGCACGCG
GTAAGAAGCACTCAATCGTCATCGTTGCTGAAGGTGCAGGAAGTGCGGAAGAAATCGGCAAATTGATTGCTGAGAAGACG
AACTCAGAAACACGCGTCACGATTCTCGGTCACATCCAACGTGGTGGTTCCCCAACTGCCGCTGACCGTGTCCTTGCAAG
TCGCATGGGTGCATACGCTGTTGAGATCTTGCTCGAAGGGAAGGCGGGCCGCGTTGTCGGAATCCGTAACAACCAAATGA
TGGATCTCGATATCGATGAGGCGCTCGACAACAACAAGCACACGATTGATATGCAATTGATTGACCTCGTCAACCAGTTG
TCGATTTAA

Upstream 100 bases:

>100_bases
ATACCCTATGCATTTTTTTCACATACTTGTGTTGTAAAACAAAGGTCAGACGTGGTATTTTATTTTCAGATTTCGTTAAA
ACACATAAGAGGTGAGCGAC

Downstream 100 bases:

>100_bases
ACGATTTTGTATGTTGCAGAGACGGGATACGGTCTAAGGCCCCCCGTTTTCTGTTTGTAAATTGCACTATATCCCCTAGG
AGGTTTTTATATTATGCGTA

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MNLKRIAVLTSGGDAPGMNAAVRAVTRKAIFEGLEVYGVYNGYQGLIEGDLVELNLGSVGDIIQRGGTFLRSARCPEFRT
EEGRAKAVEQLKKFNIDALVVIGGDGSYRGAQKLTELGFPTIGLPGTIDNDIPGTDATIGFDTALNTALDAIDKIRDTAS
SHERTYVIEVMGRDAGDIALYSGLAGGAESILVPERPEDLNAIVERINHGIARGKKHSIVIVAEGAGSAEEIGKLIAEKT
NSETRVTILGHIQRGGSPTAADRVLASRMGAYAVEILLEGKAGRVVGIRNNQMMDLDIDEALDNNKHTIDMQLIDLVNQL
SI

Sequences:

>Translated_322_residues
MNLKRIAVLTSGGDAPGMNAAVRAVTRKAIFEGLEVYGVYNGYQGLIEGDLVELNLGSVGDIIQRGGTFLRSARCPEFRT
EEGRAKAVEQLKKFNIDALVVIGGDGSYRGAQKLTELGFPTIGLPGTIDNDIPGTDATIGFDTALNTALDAIDKIRDTAS
SHERTYVIEVMGRDAGDIALYSGLAGGAESILVPERPEDLNAIVERINHGIARGKKHSIVIVAEGAGSAEEIGKLIAEKT
NSETRVTILGHIQRGGSPTAADRVLASRMGAYAVEILLEGKAGRVVGIRNNQMMDLDIDEALDNNKHTIDMQLIDLVNQL
SI
>Mature_322_residues
MNLKRIAVLTSGGDAPGMNAAVRAVTRKAIFEGLEVYGVYNGYQGLIEGDLVELNLGSVGDIIQRGGTFLRSARCPEFRT
EEGRAKAVEQLKKFNIDALVVIGGDGSYRGAQKLTELGFPTIGLPGTIDNDIPGTDATIGFDTALNTALDAIDKIRDTAS
SHERTYVIEVMGRDAGDIALYSGLAGGAESILVPERPEDLNAIVERINHGIARGKKHSIVIVAEGAGSAEEIGKLIAEKT
NSETRVTILGHIQRGGSPTAADRVLASRMGAYAVEILLEGKAGRVVGIRNNQMMDLDIDEALDNNKHTIDMQLIDLVNQL
SI

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI48762920, Length=358, Percent_Identity=43.2960893854749, Blast_Score=250, Evalue=1e-66,
Organism=Homo sapiens, GI266453619, Length=341, Percent_Identity=43.9882697947214, Blast_Score=248, Evalue=7e-66,
Organism=Homo sapiens, GI11321601, Length=349, Percent_Identity=43.8395415472779, Blast_Score=247, Evalue=8e-66,
Organism=Homo sapiens, GI266453768, Length=341, Percent_Identity=43.9882697947214, Blast_Score=247, Evalue=1e-65,
Organism=Homo sapiens, GI266453748, Length=341, Percent_Identity=43.9882697947214, Blast_Score=247, Evalue=1e-65,
Organism=Homo sapiens, GI4505749, Length=341, Percent_Identity=43.9882697947214, Blast_Score=247, Evalue=1e-65,
Organism=Escherichia coli, GI1790350, Length=318, Percent_Identity=58.1761006289308, Blast_Score=367, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI25147584, Length=311, Percent_Identity=45.3376205787781, Blast_Score=239, Evalue=1e-63,
Organism=Caenorhabditis elegans, GI17558788, Length=310, Percent_Identity=37.741935483871, Blast_Score=198, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI72003601, Length=289, Percent_Identity=34.6020761245675, Blast_Score=168, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6323861, Length=315, Percent_Identity=40, Blast_Score=212, Evalue=6e-56,
Organism=Saccharomyces cerevisiae, GI6321679, Length=346, Percent_Identity=39.0173410404624, Blast_Score=208, Evalue=9e-55,
Organism=Drosophila melanogaster, GI17647809, Length=341, Percent_Identity=42.8152492668622, Blast_Score=244, Evalue=7e-65,
Organism=Drosophila melanogaster, GI24652337, Length=341, Percent_Identity=42.8152492668622, Blast_Score=244, Evalue=7e-65,
Organism=Drosophila melanogaster, GI28573326, Length=341, Percent_Identity=42.2287390029325, Blast_Score=243, Evalue=2e-64,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 34292; Mature: 34292

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKRIAVLTSGGDAPGMNAAVRAVTRKAIFEGLEVYGVYNGYQGLIEGDLVELNLGSVG
CCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCHHEEEEECCCCCCCCCCEEEEECCCHH
DIIQRGGTFLRSARCPEFRTEEGRAKAVEQLKKFNIDALVVIGGDGSYRGAQKLTELGFP
HHHHCCCHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCC
TIGLPGTIDNDIPGTDATIGFDTALNTALDAIDKIRDTASSHERTYVIEVMGRDAGDIAL
CCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEE
YSGLAGGAESILVPERPEDLNAIVERINHGIARGKKHSIVIVAEGAGSAEEIGKLIAEKT
ECCCCCCCCCEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCC
NSETRVTILGHIQRGGSPTAADRVLASRMGAYAVEILLEGKAGRVVGIRNNQMMDLDIDE
CCCEEEEEEEEECCCCCCHHHHHHHHHHHHHEEEEEEEECCCCCEEEECCCEEEECCHHH
ALDNNKHTIDMQLIDLVNQLSI
HHCCCCEEEHHHHHHHHHHHCC
>Mature Secondary Structure
MNLKRIAVLTSGGDAPGMNAAVRAVTRKAIFEGLEVYGVYNGYQGLIEGDLVELNLGSVG
CCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCHHEEEEECCCCCCCCCCEEEEECCCHH
DIIQRGGTFLRSARCPEFRTEEGRAKAVEQLKKFNIDALVVIGGDGSYRGAQKLTELGFP
HHHHCCCHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCC
TIGLPGTIDNDIPGTDATIGFDTALNTALDAIDKIRDTASSHERTYVIEVMGRDAGDIAL
CCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEE
YSGLAGGAESILVPERPEDLNAIVERINHGIARGKKHSIVIVAEGAGSAEEIGKLIAEKT
ECCCCCCCCCEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCC
NSETRVTILGHIQRGGSPTAADRVLASRMGAYAVEILLEGKAGRVVGIRNNQMMDLDIDE
CCCEEEEEEEEECCCCCCHHHHHHHHHHHHHEEEEEEEECCCCCEEEECCCEEEECCHHH
ALDNNKHTIDMQLIDLVNQLSI
HHCCCCEEEHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA