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The map label for this gene is pyk [H]

Identifier: 229916399

GI number: 229916399

Start: 621262

End: 623004

Strand: Reverse

Name: pyk [H]

Synonym: EAT1b_0669

Alternate gene names: 229916399

Gene position: 623004-621262 (Counterclockwise)

Preceding gene: 229916400

Following gene: 229916398

Centisome position: 20.77

GC content: 49.68

Gene sequence:

>1743_bases
ATGCGTAGAACGAAAATTGTATGTACCATTGGACCGGCTTCTGAGAAGCGTCTTCCTGAGATGATTGAAGCTGGGATGAA
CGTTGCCCGTCTCAACTTCTCACACGGTGACTATGAAGAGCACGGCGCTCGTATCCGTGACATCCGTGAAGCAGCGAAAG
CAGCGGGTAAAGTTGTCACTGTTCTTCTCGACACAAAAGGTCCAGAAATCCGCACGCATACATTCGAAGACGGTAAGGCA
CTTCTTGAAAAAGGAAAAGAAGTCACGATCGTCTCGACGGAAGAAATCGTCGGAACGAAAGATCGTTTCTCTGTGACTTA
CGAAGGACTCTACGATGATGTGGAAGTCGGTTCACACATCATGCTCGACGACGGTCTCATCGGACTCCGTGTCGTTGAAA
AACTTGATAACCGTGATCTCCGTTGCGTCATCGAAAACGAAGGGATCATCAAGACGAAGAAAGGTGTTAACTTGCCAAAC
GTCAAGGTAAACCTTCCTGCCCTCACGGACAAAGATATCGCGGACATCGAGTTCGGGATCTCACAAGACATTGACGTGAT
TGCAGCATCATTCGTACGTCGTGCATCTGACGTAGTTGAAATTCGTCAACTTCTCGAGAAGCACAACGCGTCTCACATCA
AGATTTACCCGAAAATCGAGAACCAAGAAGGTGTCGATAACATCGAAGAAATCTTGGCAATCTCTGACGGTTTGATGGTA
GCCCGTGGTGACCTCGGAATTGAAATCCCGACGGAAGAAGTGACACCAGTTCAAAAAGAACTCATCAAGAAGTGTAACGA
CCTCGGGAAGCCGGTCATCACAGCAACGCAAATGCTTGATTCAATGCAACGTAACCCGCGTCCGACACGTGCGGAAGCAT
CAGACGTAGCAAACGCAATCTTGGATGGAACAGACGCAGTCATGCTCTCTGGTGAAACAGCTGCAGGGGACTACCCAATC
GAATCTGTTCAAATGCAAGCTGCAATCGCAGAGCGTACAGAACGCATGCTCGACTACAAAAACTTGTTGAAAGATCGTCA
GAAGCGCAGTGAGCACACAGTGACGGATGCGATCTCACAAGCGGTTGCGCACACGGCAATCAACTTGAACGCCAAAGCAA
TCTTGACACCGACACAATCAGGTTATACGGCTGCTCGTACAGCGAAGTATCGTCCTGAAGCGAACATCATCGCGGTGACA
GAATCGGAGCGCGTTGCGCGTCAATTGAACCTCGTATGGGGCGTATATCCAATCGTTGCTGAAACAATGCCGAACAATAC
GGATGAAATGTTGGTTCAAGCATCGGAAGCGGCACGTCACGCTGGATTCGTCACTGACGGTGACCTCGTTGTCATCTCAG
CTGGTATTCCTGCAGCGACTGCTGGTACGACGAACATGATGAAAGTCCACATCGTTGGTCACGCTCTCGCAGACGGACGT
GGAATCGGTGAGCGCGGTGTCGTTGGAGAAGTCGTTGTCGCAAAAAATGCGGATGAAGCAAACGCAAAAGCGAAAGACGG
CATGATCCTTGTCGCACCATTCACAGATAAAGAAATGATGCCTGCGATTGAAAAAGCGGCTGGAATCATCACGGAAGACG
GTGGATTGACGAGCCATGCTGGGATCGTTGGACCATCACTTCGCAAGCCGGTCATCGTAGGTGTTGACGCAGCGACAACA
TCGCTTCGTGACGGTCAGATGATTTCAATCGACCCACTCACAGGTAAAATCTACAACGCATAA

Upstream 100 bases:

>100_bases
GATTTAAACGATTTTGTATGTTGCAGAGACGGGATACGGTCTAAGGCCCCCCGTTTTCTGTTTGTAAATTGCACTATATC
CCCTAGGAGGTTTTTATATT

Downstream 100 bases:

>100_bases
TTGAACGACAATCGAACCGGCCTTCTACAAAGGTCGGTTCTTTTTCCGTTCGAAGAAAGGAATACGTATGAGTGCATATC
TATTTTTATTGATGCTCGTC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 580; Mature: 580

Protein sequence:

>580_residues
MRRTKIVCTIGPASEKRLPEMIEAGMNVARLNFSHGDYEEHGARIRDIREAAKAAGKVVTVLLDTKGPEIRTHTFEDGKA
LLEKGKEVTIVSTEEIVGTKDRFSVTYEGLYDDVEVGSHIMLDDGLIGLRVVEKLDNRDLRCVIENEGIIKTKKGVNLPN
VKVNLPALTDKDIADIEFGISQDIDVIAASFVRRASDVVEIRQLLEKHNASHIKIYPKIENQEGVDNIEEILAISDGLMV
ARGDLGIEIPTEEVTPVQKELIKKCNDLGKPVITATQMLDSMQRNPRPTRAEASDVANAILDGTDAVMLSGETAAGDYPI
ESVQMQAAIAERTERMLDYKNLLKDRQKRSEHTVTDAISQAVAHTAINLNAKAILTPTQSGYTAARTAKYRPEANIIAVT
ESERVARQLNLVWGVYPIVAETMPNNTDEMLVQASEAARHAGFVTDGDLVVISAGIPAATAGTTNMMKVHIVGHALADGR
GIGERGVVGEVVVAKNADEANAKAKDGMILVAPFTDKEMMPAIEKAAGIITEDGGLTSHAGIVGPSLRKPVIVGVDAATT
SLRDGQMISIDPLTGKIYNA

Sequences:

>Translated_580_residues
MRRTKIVCTIGPASEKRLPEMIEAGMNVARLNFSHGDYEEHGARIRDIREAAKAAGKVVTVLLDTKGPEIRTHTFEDGKA
LLEKGKEVTIVSTEEIVGTKDRFSVTYEGLYDDVEVGSHIMLDDGLIGLRVVEKLDNRDLRCVIENEGIIKTKKGVNLPN
VKVNLPALTDKDIADIEFGISQDIDVIAASFVRRASDVVEIRQLLEKHNASHIKIYPKIENQEGVDNIEEILAISDGLMV
ARGDLGIEIPTEEVTPVQKELIKKCNDLGKPVITATQMLDSMQRNPRPTRAEASDVANAILDGTDAVMLSGETAAGDYPI
ESVQMQAAIAERTERMLDYKNLLKDRQKRSEHTVTDAISQAVAHTAINLNAKAILTPTQSGYTAARTAKYRPEANIIAVT
ESERVARQLNLVWGVYPIVAETMPNNTDEMLVQASEAARHAGFVTDGDLVVISAGIPAATAGTTNMMKVHIVGHALADGR
GIGERGVVGEVVVAKNADEANAKAKDGMILVAPFTDKEMMPAIEKAAGIITEDGGLTSHAGIVGPSLRKPVIVGVDAATT
SLRDGQMISIDPLTGKIYNA
>Mature_580_residues
MRRTKIVCTIGPASEKRLPEMIEAGMNVARLNFSHGDYEEHGARIRDIREAAKAAGKVVTVLLDTKGPEIRTHTFEDGKA
LLEKGKEVTIVSTEEIVGTKDRFSVTYEGLYDDVEVGSHIMLDDGLIGLRVVEKLDNRDLRCVIENEGIIKTKKGVNLPN
VKVNLPALTDKDIADIEFGISQDIDVIAASFVRRASDVVEIRQLLEKHNASHIKIYPKIENQEGVDNIEEILAISDGLMV
ARGDLGIEIPTEEVTPVQKELIKKCNDLGKPVITATQMLDSMQRNPRPTRAEASDVANAILDGTDAVMLSGETAAGDYPI
ESVQMQAAIAERTERMLDYKNLLKDRQKRSEHTVTDAISQAVAHTAINLNAKAILTPTQSGYTAARTAKYRPEANIIAVT
ESERVARQLNLVWGVYPIVAETMPNNTDEMLVQASEAARHAGFVTDGDLVVISAGIPAATAGTTNMMKVHIVGHALADGR
GIGERGVVGEVVVAKNADEANAKAKDGMILVAPFTDKEMMPAIEKAAGIITEDGGLTSHAGIVGPSLRKPVIVGVDAATT
SLRDGQMISIDPLTGKIYNA

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=488, Percent_Identity=45.2868852459016, Blast_Score=388, Evalue=1e-108,
Organism=Homo sapiens, GI33286420, Length=488, Percent_Identity=45.2868852459016, Blast_Score=388, Evalue=1e-108,
Organism=Homo sapiens, GI10835121, Length=490, Percent_Identity=44.6938775510204, Blast_Score=383, Evalue=1e-106,
Organism=Homo sapiens, GI33286418, Length=488, Percent_Identity=45.2868852459016, Blast_Score=382, Evalue=1e-106,
Organism=Homo sapiens, GI32967597, Length=490, Percent_Identity=44.6938775510204, Blast_Score=382, Evalue=1e-106,
Organism=Homo sapiens, GI310128732, Length=289, Percent_Identity=48.4429065743945, Blast_Score=238, Evalue=1e-62,
Organism=Homo sapiens, GI310128730, Length=289, Percent_Identity=48.4429065743945, Blast_Score=238, Evalue=1e-62,
Organism=Homo sapiens, GI310128736, Length=237, Percent_Identity=45.5696202531646, Blast_Score=180, Evalue=3e-45,
Organism=Homo sapiens, GI310128734, Length=237, Percent_Identity=45.5696202531646, Blast_Score=180, Evalue=3e-45,
Organism=Homo sapiens, GI310128738, Length=216, Percent_Identity=44.4444444444444, Blast_Score=159, Evalue=6e-39,
Organism=Escherichia coli, GI1787965, Length=477, Percent_Identity=48.6373165618449, Blast_Score=439, Evalue=1e-124,
Organism=Escherichia coli, GI1788160, Length=482, Percent_Identity=38.5892116182573, Blast_Score=281, Evalue=9e-77,
Organism=Escherichia coli, GI1787994, Length=165, Percent_Identity=30.9090909090909, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17544584, Length=486, Percent_Identity=46.5020576131687, Blast_Score=382, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI71984413, Length=489, Percent_Identity=43.1492842535787, Blast_Score=352, Evalue=4e-97,
Organism=Caenorhabditis elegans, GI17506829, Length=489, Percent_Identity=43.1492842535787, Blast_Score=352, Evalue=4e-97,
Organism=Caenorhabditis elegans, GI71984406, Length=489, Percent_Identity=43.1492842535787, Blast_Score=351, Evalue=5e-97,
Organism=Caenorhabditis elegans, GI17506831, Length=489, Percent_Identity=42.7402862985685, Blast_Score=351, Evalue=5e-97,
Organism=Saccharomyces cerevisiae, GI6324923, Length=492, Percent_Identity=41.6666666666667, Blast_Score=342, Evalue=9e-95,
Organism=Saccharomyces cerevisiae, GI6319279, Length=487, Percent_Identity=41.8891170431211, Blast_Score=335, Evalue=9e-93,
Organism=Drosophila melanogaster, GI24648964, Length=489, Percent_Identity=43.1492842535787, Blast_Score=361, Evalue=1e-100,
Organism=Drosophila melanogaster, GI28571814, Length=489, Percent_Identity=43.1492842535787, Blast_Score=360, Evalue=1e-99,
Organism=Drosophila melanogaster, GI24648966, Length=413, Percent_Identity=44.0677966101695, Blast_Score=317, Evalue=1e-86,
Organism=Drosophila melanogaster, GI24581235, Length=486, Percent_Identity=34.5679012345679, Blast_Score=274, Evalue=1e-73,
Organism=Drosophila melanogaster, GI24646914, Length=272, Percent_Identity=43.75, Blast_Score=206, Evalue=3e-53,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 62582; Mature: 62582

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRTKIVCTIGPASEKRLPEMIEAGMNVARLNFSHGDYEEHGARIRDIREAAKAAGKVVT
CCCEEEEEEECCCCHHCCHHHHHCCCCEEEEECCCCCHHHHCHHHHHHHHHHHHCCEEEE
VLLDTKGPEIRTHTFEDGKALLEKGKEVTIVSTEEIVGTKDRFSVTYEGLYDDVEVGSHI
EEEECCCCCEEEEECCCCHHHHHCCCEEEEEEHHHHCCCCCCEEEEECCCCCCHHCCCEE
MLDDGLIGLRVVEKLDNRDLRCVIENEGIIKTKKGVNLPNVKVNLPALTDKDIADIEFGI
EECCCCHHHHHHHHHCCCCEEEEEECCCEEEECCCCCCCCEEEECCCCCCCCCHHEECCC
SQDIDVIAASFVRRASDVVEIRQLLEKHNASHIKIYPKIENQEGVDNIEEILAISDGLMV
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHCCCEEE
ARGDLGIEIPTEEVTPVQKELIKKCNDLGKPVITATQMLDSMQRNPRPTRAEASDVANAI
EECCCCEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
LDGTDAVMLSGETAAGDYPIESVQMQAAIAERTERMLDYKNLLKDRQKRSEHTVTDAISQ
HCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVAHTAINLNAKAILTPTQSGYTAARTAKYRPEANIIAVTESERVARQLNLVWGVYPIVA
HHHHHHEECCCEEEEECCCCCCCHHHCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHH
ETMPNNTDEMLVQASEAARHAGFVTDGDLVVISAGIPAATAGTTNMMKVHIVGHALADGR
HHCCCCHHHHHHHHHHHHHHCCCEECCCEEEEECCCCCCCCCCCCEEEEEEEEEEHHCCC
GIGERGVVGEVVVAKNADEANAKAKDGMILVAPFTDKEMMPAIEKAAGIITEDGGLTSHA
CCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCEEECCCCCCCCC
GIVGPSLRKPVIVGVDAATTSLRDGQMISIDPLTGKIYNA
CCCCCCCCCCEEEEECCHHCCCCCCCEEEEECCCCCEECC
>Mature Secondary Structure
MRRTKIVCTIGPASEKRLPEMIEAGMNVARLNFSHGDYEEHGARIRDIREAAKAAGKVVT
CCCEEEEEEECCCCHHCCHHHHHCCCCEEEEECCCCCHHHHCHHHHHHHHHHHHCCEEEE
VLLDTKGPEIRTHTFEDGKALLEKGKEVTIVSTEEIVGTKDRFSVTYEGLYDDVEVGSHI
EEEECCCCCEEEEECCCCHHHHHCCCEEEEEEHHHHCCCCCCEEEEECCCCCCHHCCCEE
MLDDGLIGLRVVEKLDNRDLRCVIENEGIIKTKKGVNLPNVKVNLPALTDKDIADIEFGI
EECCCCHHHHHHHHHCCCCEEEEEECCCEEEECCCCCCCCEEEECCCCCCCCCHHEECCC
SQDIDVIAASFVRRASDVVEIRQLLEKHNASHIKIYPKIENQEGVDNIEEILAISDGLMV
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHCCCEEE
ARGDLGIEIPTEEVTPVQKELIKKCNDLGKPVITATQMLDSMQRNPRPTRAEASDVANAI
EECCCCEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
LDGTDAVMLSGETAAGDYPIESVQMQAAIAERTERMLDYKNLLKDRQKRSEHTVTDAISQ
HCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVAHTAINLNAKAILTPTQSGYTAARTAKYRPEANIIAVTESERVARQLNLVWGVYPIVA
HHHHHHEECCCEEEEECCCCCCCHHHCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHH
ETMPNNTDEMLVQASEAARHAGFVTDGDLVVISAGIPAATAGTTNMMKVHIVGHALADGR
HHCCCCHHHHHHHHHHHHHHCCCEECCCEEEEECCCCCCCCCCCCEEEEEEEEEEHHCCC
GIGERGVVGEVVVAKNADEANAKAKDGMILVAPFTDKEMMPAIEKAAGIITEDGGLTSHA
CCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCEEECCCCCCCCC
GIVGPSLRKPVIVGVDAATTSLRDGQMISIDPLTGKIYNA
CCCCCCCCCCEEEEECCHHCCCCCCCEEEEECCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7549104 [H]