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The map label for this gene is pheS

Identifier: 229916371

GI number: 229916371

Start: 595115

End: 596137

Strand: Reverse

Name: pheS

Synonym: EAT1b_0641

Alternate gene names: 229916371

Gene position: 596137-595115 (Counterclockwise)

Preceding gene: 229916372

Following gene: 229916370

Centisome position: 19.87

GC content: 48.19

Gene sequence:

>1023_bases
ATGAAAGAACAATTAGAACAGTTACAAGTGGAAGCGTTAGAAGAAATCAAACAGGCATCCACACAAAAATCATTGAATGA
CGTCCGCATCAAATATTTAGGGAAAAAAGGACCAATGACTGAAGTGCTCCGTGGCATGGGTAAATTATCTGCCGAGGAAC
GTCCGATTATCGGTGAGTTGGCGAATACCGTTCGTACCGCGATTCAAAGCACACTCGATGCTCGAATCGAAGAGGTGAAG
GCGATTGAATTAGAGGAAAAGTTAAAAGCGGAAACGCTCGATGTGACTCTACCGGGGCGGACATCGACTCCTGGCCACAG
TCATCTCTTACAACAAATCATCGACGAGATGGAAGACTTGTTCGTTGGACTCGGATATACGATTGCTGAAGGTCCTGAAG
TCGAAACCGATCTCTACAACTTCGAAATGCTCAACTTGCCGAAAGACCACCCTGCACGAGACATGCAGGACTCGTTCTAT
ATCACGGAAGAGACGTTACTTCGTACTCATACGTCTCCTGTACAGGCTCGCACGATGTTGGAGGCGAACGGAAAACCGAT
TCGCATCATCTGCCCAGGTAAAGTGTATCGTCGTGATGAAGACGATGCGACGCATTCGCATCAATTCATGCAAATCGAAG
GGTTGGTTGTCGATGAATCCATCTCGATGGCTGACTTGAAAGGGACACTCGAAGTGTTCGTGAAGCAGTTGTTCGGAGAA
ACGCGCGAAATTCGTCTCCGTCCAAGCTTCTTCCCGTTCACGGAACCATCGGTCGAAGTCGACATCTCTTGCTTCAAGTG
TGGCGGGAAAGGATGTAACGTCTGTAAAGGAACCGGTTGGATTGAAATTCTCGGAGGCGGAATGGTCCATCCGCATGTCT
TGGAGATGGCTGGCTATGACTCATCAAAAGTGTCAGGGTTCGCATTCGGAATCGGAGTGGAGCGTATGGCGATGCTCAAA
CACGGCGTCGACGACATCCGCCATTTCTACACAAATGACTTACGTTTCATCGAGCAATTCTAA

Upstream 100 bases:

>100_bases
AAGTGGGCCATGAATTGGCCAAGTAGGGTGGTACCGCGATGATTCGTCCCTTCAGTCAACGACTGAAGGGACTTTTTTAT
GTAGAGGAGGAAGCGAACAC

Downstream 100 bases:

>100_bases
GGAGGAAGACAGATGTTACTTTCAAAAAAATGGTTGAATCAATATATTGATGTATCCGATTTAAGCGGCGAAGCGCTCGG
TGACTTGATTACGAAGAACG

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MKEQLEQLQVEALEEIKQASTQKSLNDVRIKYLGKKGPMTEVLRGMGKLSAEERPIIGELANTVRTAIQSTLDARIEEVK
AIELEEKLKAETLDVTLPGRTSTPGHSHLLQQIIDEMEDLFVGLGYTIAEGPEVETDLYNFEMLNLPKDHPARDMQDSFY
ITEETLLRTHTSPVQARTMLEANGKPIRIICPGKVYRRDEDDATHSHQFMQIEGLVVDESISMADLKGTLEVFVKQLFGE
TREIRLRPSFFPFTEPSVEVDISCFKCGGKGCNVCKGTGWIEILGGGMVHPHVLEMAGYDSSKVSGFAFGIGVERMAMLK
HGVDDIRHFYTNDLRFIEQF

Sequences:

>Translated_340_residues
MKEQLEQLQVEALEEIKQASTQKSLNDVRIKYLGKKGPMTEVLRGMGKLSAEERPIIGELANTVRTAIQSTLDARIEEVK
AIELEEKLKAETLDVTLPGRTSTPGHSHLLQQIIDEMEDLFVGLGYTIAEGPEVETDLYNFEMLNLPKDHPARDMQDSFY
ITEETLLRTHTSPVQARTMLEANGKPIRIICPGKVYRRDEDDATHSHQFMQIEGLVVDESISMADLKGTLEVFVKQLFGE
TREIRLRPSFFPFTEPSVEVDISCFKCGGKGCNVCKGTGWIEILGGGMVHPHVLEMAGYDSSKVSGFAFGIGVERMAMLK
HGVDDIRHFYTNDLRFIEQF
>Mature_340_residues
MKEQLEQLQVEALEEIKQASTQKSLNDVRIKYLGKKGPMTEVLRGMGKLSAEERPIIGELANTVRTAIQSTLDARIEEVK
AIELEEKLKAETLDVTLPGRTSTPGHSHLLQQIIDEMEDLFVGLGYTIAEGPEVETDLYNFEMLNLPKDHPARDMQDSFY
ITEETLLRTHTSPVQARTMLEANGKPIRIICPGKVYRRDEDDATHSHQFMQIEGLVVDESISMADLKGTLEVFVKQLFGE
TREIRLRPSFFPFTEPSVEVDISCFKCGGKGCNVCKGTGWIEILGGGMVHPHVLEMAGYDSSKVSGFAFGIGVERMAMLK
HGVDDIRHFYTNDLRFIEQF

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=269, Percent_Identity=29.368029739777, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI5729820, Length=242, Percent_Identity=32.2314049586777, Blast_Score=115, Evalue=7e-26,
Organism=Escherichia coli, GI1788007, Length=337, Percent_Identity=47.7744807121662, Blast_Score=316, Evalue=1e-87,
Organism=Caenorhabditis elegans, GI32563657, Length=275, Percent_Identity=29.0909090909091, Blast_Score=124, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17508957, Length=275, Percent_Identity=29.0909090909091, Blast_Score=124, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI32566635, Length=279, Percent_Identity=33.3333333333333, Blast_Score=122, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6321087, Length=296, Percent_Identity=29.0540540540541, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6325304, Length=285, Percent_Identity=28.4210526315789, Blast_Score=107, Evalue=3e-24,
Organism=Drosophila melanogaster, GI18858079, Length=309, Percent_Identity=29.126213592233, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI17137424, Length=249, Percent_Identity=30.5220883534137, Blast_Score=106, Evalue=2e-23,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_EXISA (C4L450)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002885017.1
- GeneID:   7871380
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_0641
- OMA:   FRASYFP
- ProtClustDB:   PRK00488
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 38224; Mature: 38224

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEQLEQLQVEALEEIKQASTQKSLNDVRIKYLGKKGPMTEVLRGMGKLSAEERPIIGEL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHCCCCCCCHHHHH
ANTVRTAIQSTLDARIEEVKAIELEEKLKAETLDVTLPGRTSTPGHSHLLQQIIDEMEDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHH
FVGLGYTIAEGPEVETDLYNFEMLNLPKDHPARDMQDSFYITEETLLRTHTSPVQARTML
HHHCCCEECCCCCCCCHHCCEEEECCCCCCCCCCCCCCEEEEHHHHHHHCCCCHHHHHHH
EANGKPIRIICPGKVYRRDEDDATHSHQFMQIEGLVVDESISMADLKGTLEVFVKQLFGE
HCCCCEEEEECCCHHCCCCCCCCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHCC
TREIRLRPSFFPFTEPSVEVDISCFKCGGKGCNVCKGTGWIEILGGGMVHPHVLEMAGYD
CEEEEECCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCEEEEECCCCCCHHHHHHCCCC
SSKVSGFAFGIGVERMAMLKHGVDDIRHFYTNDLRFIEQF
CCCCCCEEEHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKEQLEQLQVEALEEIKQASTQKSLNDVRIKYLGKKGPMTEVLRGMGKLSAEERPIIGEL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHCCCCCCCHHHHH
ANTVRTAIQSTLDARIEEVKAIELEEKLKAETLDVTLPGRTSTPGHSHLLQQIIDEMEDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHH
FVGLGYTIAEGPEVETDLYNFEMLNLPKDHPARDMQDSFYITEETLLRTHTSPVQARTML
HHHCCCEECCCCCCCCHHCCEEEECCCCCCCCCCCCCCEEEEHHHHHHHCCCCHHHHHHH
EANGKPIRIICPGKVYRRDEDDATHSHQFMQIEGLVVDESISMADLKGTLEVFVKQLFGE
HCCCCEEEEECCCHHCCCCCCCCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHCC
TREIRLRPSFFPFTEPSVEVDISCFKCGGKGCNVCKGTGWIEILGGGMVHPHVLEMAGYD
CEEEEECCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCEEEEECCCCCCHHHHHHCCCC
SSKVSGFAFGIGVERMAMLKHGVDDIRHFYTNDLRFIEQF
CCCCCCEEEHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA