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The map label for this gene is pheT [H]

Identifier: 229916370

GI number: 229916370

Start: 592727

End: 595102

Strand: Reverse

Name: pheT [H]

Synonym: EAT1b_0640

Alternate gene names: 229916370

Gene position: 595102-592727 (Counterclockwise)

Preceding gene: 229916371

Following gene: 229916366

Centisome position: 19.84

GC content: 50.42

Gene sequence:

>2376_bases
ATGTTACTTTCAAAAAAATGGTTGAATCAATATATTGATGTATCCGATTTAAGCGGCGAAGCGCTCGGTGACTTGATTAC
GAAGAACGGAATCGAAGTCGAAAGTGTGACAAATCGTGGGGAAGGACTTTCTGGGATCGTCGTCGGTCGGGTCCTTTCAT
GTGAGAAACATCCGGAAGCGGATAAGTTAAACGTGACGAAAGTGGACATCGGAGAATGTGACCCTGTTCAAATCGTCTGT
GGCGCACCGAACGTGCGCGAAGGGCAACATGTGATTGTGGCCAAAGTTGGCGCACGCTTACCAGGACTTAAAATCAAAAA
AGCAAAACTTCGTGGTGTCGAATCACAAGGGATGATTTGTTCACTCGAAGAGCTCGGCTTTGAGAAAAAACAAATTCGGG
AAGACGAGCAAGATGGAATCCACACATTCCGTGAATCCGTACAAGTTGGTGCAGATGTGCTCGATTTACTCGACTTGAAC
GATGAAGTCATCGAACTCGGCTTGACTCCGAACCGTTCAGATTGCTTGAGCCTCTATGGAATCATTCATGAGGTTGCGGC
CATCTTAGAGCGTCCATACACGTTGCCGACGGCAGATGTCACGTCATCATCACCATCGGAGGTGTCTATCGAACTTGCGA
CACCGAACTGTTCATATTACGCAGCTCGTGAAGTCAAAGGGGTAACGATTGCAGAATCGCCACAATGGCTAAAGAATATC
TTGATCGCAGAAGGTGTCCGTCCAATCAACAACATTGTCGACGTGACGAACTACGTCATGCTTGAAATCGGACAACCGCT
TCACGCATTCGATGCGAAGAAACTCGGCAACTCGATTGTGGTCCGTCAAGCCGAAAATGGAGAAGAAATGGTCACACTCG
ACGACGTGACGCGCACGCTCGATTCATCGATGATGGTCATCACAGACGGACAAGCTCCAGTCGCCATCGCAGGCGTCATG
GGTGGTGCCAATACAGAGGTAGATCTTTCAACGACAGATATCATTTTAGAGTCAGCGTACTTTGATCCGGCATCCGTACG
GAAGACGAGCCGTACGCTCGGTCTCCGTTCAGATTCAAGCGCTCGTTTCGAAAAAGGCGTTGACCCAGAACGTGTCATGC
TCGCGCTCGATCGCGCCGCGCAACTGATTGTCGATCTCGGTGGAGGAGCCATTCATGATGCGGTGCGTTCAGGTGAAGCC
GAATCACGTGCCCGAGAAATCCAAGTGTCGATTGACTATATCAACAAGCGCTTAGGGATGACACTCGATCGTCAGACAAT
CGTTCACATCCTGGAGCGTCTCGGTCTTGCGACAGAAGGGGATGATGTGCTGACCGTACACATTCCATCGCGCCGTCCGG
ACTTAGAGCTTCCGGCTGATATCACAGAAGAAGTGGCACGTCTATACGGATATGATAGTCTCCCGTCGACGTTACCTGCC
TCTCAAATGAAAGGCTATTTGCCAGCAATCAACGTATTGCGCAGGCAGACACGTCGCTTGTTGCAAGGAGTCGGCTTATC
ACAAGCCATTACCTACTCGTTAACGAGTGAGGCACATGCGACACGTTTCGGTGGAGAAACCGATCAGCTCGTTCGATTGG
CGATGCCGATGAGTGAAGAACGTTCTGTCCTTCGTACGTCGCTCGTCCCTGGGTTGTTAGAGGCGGCACGTTACAACACG
GCGCGTCAACAATCGAACGTCGCACTTTACGAAACAGGACGCGTCTATACGGAGCACGGAGAAACGTTACCACGTGAGAA
AGAACGGGTGGCTGGTGTCGTCACAGGTCTATGGATGGATCATCGTTCGCAAGGGACACGGATCCCGGTCGACTATTTCG
TCGCAAAAGGGATTGTCGACACGTTAGTCCAAACGCTTCGACTAGATGTCACGTATGAGGCCGTATCGATGCCTGATATG
CATCCTGGTCGTACGGCAAACATCTTGATGGACGGAGAGGTCATCGGATATGTCGGACAAGTGCATCCAGGAACATCGAA
AGACGTCTACGGTATGAAGGAAGTGTATGTATTCGAACTCGACCTCTTGGCACTTCGTGGAGACGAGACACTTCTTTATC
AAGACGTACCACGTTATCCATCGATTTCACGAGATTTGGCACTCGTCTTATCACGTGAGATTCCGGCTGGGACAGTGGAA
CAAACGATCCGCCAAGCGGCTGGTCCACTCTTGATTGACCTCGTCTTGTTTGACGTCTATACAGGGGAGAATGTTGGAGC
GGATGAGAAATCGATTGCCTTCTCACTCAAGTTCCAAGACCCGTCACGAACGTTACAAGATGAAGAAGTAACGGCAGCAT
ATGAAACAATTGTCGAGGCGGTCCGTGCGACACACGGCGCTGAACTTCGAGCGTAA

Upstream 100 bases:

>100_bases
AGTGGAGCGTATGGCGATGCTCAAACACGGCGTCGACGACATCCGCCATTTCTACACAAATGACTTACGTTTCATCGAGC
AATTCTAAGGAGGAAGACAG

Downstream 100 bases:

>100_bases
AAAATATGGAGCGTTGTCCACCTGTGACAACGCTCCTTTTATTATTGAATCTCGAGTGCGAGATCACCGAACTTGGTCCA
TCCGACAAGACTCGTCACAC

Product: phenylalanyl-tRNA synthetase, subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 791; Mature: 791

Protein sequence:

>791_residues
MLLSKKWLNQYIDVSDLSGEALGDLITKNGIEVESVTNRGEGLSGIVVGRVLSCEKHPEADKLNVTKVDIGECDPVQIVC
GAPNVREGQHVIVAKVGARLPGLKIKKAKLRGVESQGMICSLEELGFEKKQIREDEQDGIHTFRESVQVGADVLDLLDLN
DEVIELGLTPNRSDCLSLYGIIHEVAAILERPYTLPTADVTSSSPSEVSIELATPNCSYYAAREVKGVTIAESPQWLKNI
LIAEGVRPINNIVDVTNYVMLEIGQPLHAFDAKKLGNSIVVRQAENGEEMVTLDDVTRTLDSSMMVITDGQAPVAIAGVM
GGANTEVDLSTTDIILESAYFDPASVRKTSRTLGLRSDSSARFEKGVDPERVMLALDRAAQLIVDLGGGAIHDAVRSGEA
ESRAREIQVSIDYINKRLGMTLDRQTIVHILERLGLATEGDDVLTVHIPSRRPDLELPADITEEVARLYGYDSLPSTLPA
SQMKGYLPAINVLRRQTRRLLQGVGLSQAITYSLTSEAHATRFGGETDQLVRLAMPMSEERSVLRTSLVPGLLEAARYNT
ARQQSNVALYETGRVYTEHGETLPREKERVAGVVTGLWMDHRSQGTRIPVDYFVAKGIVDTLVQTLRLDVTYEAVSMPDM
HPGRTANILMDGEVIGYVGQVHPGTSKDVYGMKEVYVFELDLLALRGDETLLYQDVPRYPSISRDLALVLSREIPAGTVE
QTIRQAAGPLLIDLVLFDVYTGENVGADEKSIAFSLKFQDPSRTLQDEEVTAAYETIVEAVRATHGAELRA

Sequences:

>Translated_791_residues
MLLSKKWLNQYIDVSDLSGEALGDLITKNGIEVESVTNRGEGLSGIVVGRVLSCEKHPEADKLNVTKVDIGECDPVQIVC
GAPNVREGQHVIVAKVGARLPGLKIKKAKLRGVESQGMICSLEELGFEKKQIREDEQDGIHTFRESVQVGADVLDLLDLN
DEVIELGLTPNRSDCLSLYGIIHEVAAILERPYTLPTADVTSSSPSEVSIELATPNCSYYAAREVKGVTIAESPQWLKNI
LIAEGVRPINNIVDVTNYVMLEIGQPLHAFDAKKLGNSIVVRQAENGEEMVTLDDVTRTLDSSMMVITDGQAPVAIAGVM
GGANTEVDLSTTDIILESAYFDPASVRKTSRTLGLRSDSSARFEKGVDPERVMLALDRAAQLIVDLGGGAIHDAVRSGEA
ESRAREIQVSIDYINKRLGMTLDRQTIVHILERLGLATEGDDVLTVHIPSRRPDLELPADITEEVARLYGYDSLPSTLPA
SQMKGYLPAINVLRRQTRRLLQGVGLSQAITYSLTSEAHATRFGGETDQLVRLAMPMSEERSVLRTSLVPGLLEAARYNT
ARQQSNVALYETGRVYTEHGETLPREKERVAGVVTGLWMDHRSQGTRIPVDYFVAKGIVDTLVQTLRLDVTYEAVSMPDM
HPGRTANILMDGEVIGYVGQVHPGTSKDVYGMKEVYVFELDLLALRGDETLLYQDVPRYPSISRDLALVLSREIPAGTVE
QTIRQAAGPLLIDLVLFDVYTGENVGADEKSIAFSLKFQDPSRTLQDEEVTAAYETIVEAVRATHGAELRA
>Mature_791_residues
MLLSKKWLNQYIDVSDLSGEALGDLITKNGIEVESVTNRGEGLSGIVVGRVLSCEKHPEADKLNVTKVDIGECDPVQIVC
GAPNVREGQHVIVAKVGARLPGLKIKKAKLRGVESQGMICSLEELGFEKKQIREDEQDGIHTFRESVQVGADVLDLLDLN
DEVIELGLTPNRSDCLSLYGIIHEVAAILERPYTLPTADVTSSSPSEVSIELATPNCSYYAAREVKGVTIAESPQWLKNI
LIAEGVRPINNIVDVTNYVMLEIGQPLHAFDAKKLGNSIVVRQAENGEEMVTLDDVTRTLDSSMMVITDGQAPVAIAGVM
GGANTEVDLSTTDIILESAYFDPASVRKTSRTLGLRSDSSARFEKGVDPERVMLALDRAAQLIVDLGGGAIHDAVRSGEA
ESRAREIQVSIDYINKRLGMTLDRQTIVHILERLGLATEGDDVLTVHIPSRRPDLELPADITEEVARLYGYDSLPSTLPA
SQMKGYLPAINVLRRQTRRLLQGVGLSQAITYSLTSEAHATRFGGETDQLVRLAMPMSEERSVLRTSLVPGLLEAARYNT
ARQQSNVALYETGRVYTEHGETLPREKERVAGVVTGLWMDHRSQGTRIPVDYFVAKGIVDTLVQTLRLDVTYEAVSMPDM
HPGRTANILMDGEVIGYVGQVHPGTSKDVYGMKEVYVFELDLLALRGDETLLYQDVPRYPSISRDLALVLSREIPAGTVE
QTIRQAAGPLLIDLVLFDVYTGENVGADEKSIAFSLKFQDPSRTLQDEEVTAAYETIVEAVRATHGAELRA

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI124028525, Length=272, Percent_Identity=26.4705882352941, Blast_Score=81, Evalue=3e-15,
Organism=Escherichia coli, GI1788006, Length=804, Percent_Identity=35.9452736318408, Blast_Score=456, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI17533403, Length=274, Percent_Identity=27.007299270073, Blast_Score=81, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323089, Length=292, Percent_Identity=26.7123287671233, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21355211, Length=279, Percent_Identity=30.1075268817204, Blast_Score=98, Evalue=2e-20,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 86524; Mature: 86524

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLSKKWLNQYIDVSDLSGEALGDLITKNGIEVESVTNRGEGLSGIVVGRVLSCEKHPEA
CCCCHHHHHHHCCCCCCCCHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHCCCCCCCC
DKLNVTKVDIGECDPVQIVCGAPNVREGQHVIVAKVGARLPGLKIKKAKLRGVESQGMIC
CCEEEEEEECCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCCEEEHHHHCCCCCCCCEE
SLEELGFEKKQIREDEQDGIHTFRESVQVGADVLDLLDLNDEVIELGLTPNRSDCLSLYG
EHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEECCCCCHHHHHHHHH
IIHEVAAILERPYTLPTADVTSSSPSEVSIELATPNCSYYAAREVKGVTIAESPQWLKNI
HHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEHHCCCCCEEECCHHHHHHH
LIAEGVRPINNIVDVTNYVMLEIGQPLHAFDAKKLGNSIVVRQAENGEEMVTLDDVTRTL
HHHCCCCHHHHHHHHHHHEEEECCCCHHHHHHHHCCCEEEEEECCCCCCEEEHHHHHHHH
DSSMMVITDGQAPVAIAGVMGGANTEVDLSTTDIILESAYFDPASVRKTSRTLGLRSDSS
CCCEEEEECCCCCEEEEEEECCCCCEEECCHHHHEEECCCCCHHHHHHHHHHHCCCCCCC
ARFEKGVDPERVMLALDRAAQLIVDLGGGAIHDAVRSGEAESRAREIQVSIDYINKRLGM
HHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHCCEEEEEEHHHHHHHHCC
TLDRQTIVHILERLGLATEGDDVLTVHIPSRRPDLELPADITEEVARLYGYDSLPSTLPA
EECHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCH
SQMKGYLPAINVLRRQTRRLLQGVGLSQAITYSLTSEAHATRFGGETDQLVRLAMPMSEE
HHHCCCHHHHHHHHHHHHHHHHCCCCCHHEEEEECCCHHHHHCCCCHHHHHHHHCCCCHH
RSVLRTSLVPGLLEAARYNTARQQSNVALYETGRVYTEHGETLPREKERVAGVVTGLWMD
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEECCCCCCCCHHHHHHHHHHHHHHH
HRSQGTRIPVDYFVAKGIVDTLVQTLRLDVTYEAVSMPDMHPGRTANILMDGEVIGYVGQ
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCEEEEEECCCEEEEEEC
VHPGTSKDVYGMKEVYVFELDLLALRGDETLLYQDVPRYPSISRDLALVLSREIPAGTVE
CCCCCCCCCCCCCEEEEEEEEEEEECCCCEEEECCCCCCCCCCHHHHHHHHCCCCCCHHH
QTIRQAAGPLLIDLVLFDVYTGENVGADEKSIAFSLKFQDPSRTLQDEEVTAAYETIVEA
HHHHHHCCCCEEEHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHH
VRATHGAELRA
HHHHCCCCCCC
>Mature Secondary Structure
MLLSKKWLNQYIDVSDLSGEALGDLITKNGIEVESVTNRGEGLSGIVVGRVLSCEKHPEA
CCCCHHHHHHHCCCCCCCCHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHCCCCCCCC
DKLNVTKVDIGECDPVQIVCGAPNVREGQHVIVAKVGARLPGLKIKKAKLRGVESQGMIC
CCEEEEEEECCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCCEEEHHHHCCCCCCCCEE
SLEELGFEKKQIREDEQDGIHTFRESVQVGADVLDLLDLNDEVIELGLTPNRSDCLSLYG
EHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEECCCCCHHHHHHHHH
IIHEVAAILERPYTLPTADVTSSSPSEVSIELATPNCSYYAAREVKGVTIAESPQWLKNI
HHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEHHCCCCCEEECCHHHHHHH
LIAEGVRPINNIVDVTNYVMLEIGQPLHAFDAKKLGNSIVVRQAENGEEMVTLDDVTRTL
HHHCCCCHHHHHHHHHHHEEEECCCCHHHHHHHHCCCEEEEEECCCCCCEEEHHHHHHHH
DSSMMVITDGQAPVAIAGVMGGANTEVDLSTTDIILESAYFDPASVRKTSRTLGLRSDSS
CCCEEEEECCCCCEEEEEEECCCCCEEECCHHHHEEECCCCCHHHHHHHHHHHCCCCCCC
ARFEKGVDPERVMLALDRAAQLIVDLGGGAIHDAVRSGEAESRAREIQVSIDYINKRLGM
HHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHCCEEEEEEHHHHHHHHCC
TLDRQTIVHILERLGLATEGDDVLTVHIPSRRPDLELPADITEEVARLYGYDSLPSTLPA
EECHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCH
SQMKGYLPAINVLRRQTRRLLQGVGLSQAITYSLTSEAHATRFGGETDQLVRLAMPMSEE
HHHCCCHHHHHHHHHHHHHHHHCCCCCHHEEEEECCCHHHHHCCCCHHHHHHHHCCCCHH
RSVLRTSLVPGLLEAARYNTARQQSNVALYETGRVYTEHGETLPREKERVAGVVTGLWMD
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEECCCCCCCCHHHHHHHHHHHHHHH
HRSQGTRIPVDYFVAKGIVDTLVQTLRLDVTYEAVSMPDMHPGRTANILMDGEVIGYVGQ
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCEEEEEECCCEEEEEEC
VHPGTSKDVYGMKEVYVFELDLLALRGDETLLYQDVPRYPSISRDLALVLSREIPAGTVE
CCCCCCCCCCCCCEEEEEEEEEEEECCCCEEEECCCCCCCCCCHHHHHHHHCCCCCCHHH
QTIRQAAGPLLIDLVLFDVYTGENVGADEKSIAFSLKFQDPSRTLQDEEVTAAYETIVEA
HHHHHHCCCCEEEHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHH
VRATHGAELRA
HHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA