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The map label for this gene is rsmE [H]

Identifier: 229916343

GI number: 229916343

Start: 564812

End: 565576

Strand: Reverse

Name: rsmE [H]

Synonym: EAT1b_0613

Alternate gene names: 229916343

Gene position: 565576-564812 (Counterclockwise)

Preceding gene: 229916344

Following gene: 229916341

Centisome position: 18.85

GC content: 48.89

Gene sequence:

>765_bases
ATGCAACGTTATTTTTTGACAAAAGAAGCCTTTAAAGGCGAAACGGTGACCCTTCCTTCCGACGTGGTCCATCACATCGG
TACAGTCTTACGACAAGGTCCTGGATATGAGATGGTCCTATTGGATGGTACAGGTGTTGAGTATGCCTGTGTCGTGACAT
CACTTGAGAAAAAGAGCGGGGAAGCGAAAGTCATGTCATCGCGCCAGGCGACGACGGAATTGCCGATCGAGGTGACTTTG
GCGTATGCACTACCGAAAGGAGACAAAGTCGAGCTCGTCGCCCAAAAAGCGACGGAGCTTGGCGTCCACCATCTGTTGCT
GTTCGAATCGACGCGCTCTGTTGCCAAATGGGATGGAAAAAAAGCTCCGAAAAAAGTGGAGCGGATTCAAAAGATTATGC
AAGAAGCGGCTGAACAATCCTATCGTGCCGTGGTACCGACTTGCACATATATTTCTCCGAAAGAGCTCCAACAGAAAATA
GATGGCTATGATGCCGTCGTCATCGCATATGAAGAGTCGGCAAAAGAAGGGGAACAGTCTGCTTTCGCGCAACTGTTGAC
TCAACTGAAACCGAAACAACGCGTTCTCGTCATCACGGGACCTGAAGGTGGATTCGATGTGAACGAAATCAATCAATGGA
CTCAGGCAGGAGCGACTGCATGTGCATTTGGACCACGAATTCTTCGGTCTGAGACGGCGCCGCTTTATGCATTATCTGCA
ATCTCTTTCGCGCTTGAACTGAACAGATCATATGAAAACGGATGA

Upstream 100 bases:

>100_bases
GGCTGAAGTGACGAATGCTTTGGTCGCTGCTGGATTTGAGATCGAAGAGACGCGCGTGATGGAAGATTGGGTCGCGATTA
TCGCCAAGAAGGGGTAAGTT

Downstream 100 bases:

>100_bases
CCCGATCGAGGTCATCCGTTTTTCATTTTATTTTTTGTTGATTGTAATCACTTTCTTCGTGACGTTTCCGCCAAGGTCGG
TCACTTCGAATGTGAAGGTA

Product: hypothetical protein

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MQRYFLTKEAFKGETVTLPSDVVHHIGTVLRQGPGYEMVLLDGTGVEYACVVTSLEKKSGEAKVMSSRQATTELPIEVTL
AYALPKGDKVELVAQKATELGVHHLLLFESTRSVAKWDGKKAPKKVERIQKIMQEAAEQSYRAVVPTCTYISPKELQQKI
DGYDAVVIAYEESAKEGEQSAFAQLLTQLKPKQRVLVITGPEGGFDVNEINQWTQAGATACAFGPRILRSETAPLYALSA
ISFALELNRSYENG

Sequences:

>Translated_254_residues
MQRYFLTKEAFKGETVTLPSDVVHHIGTVLRQGPGYEMVLLDGTGVEYACVVTSLEKKSGEAKVMSSRQATTELPIEVTL
AYALPKGDKVELVAQKATELGVHHLLLFESTRSVAKWDGKKAPKKVERIQKIMQEAAEQSYRAVVPTCTYISPKELQQKI
DGYDAVVIAYEESAKEGEQSAFAQLLTQLKPKQRVLVITGPEGGFDVNEINQWTQAGATACAFGPRILRSETAPLYALSA
ISFALELNRSYENG
>Mature_254_residues
MQRYFLTKEAFKGETVTLPSDVVHHIGTVLRQGPGYEMVLLDGTGVEYACVVTSLEKKSGEAKVMSSRQATTELPIEVTL
AYALPKGDKVELVAQKATELGVHHLLLFESTRSVAKWDGKKAPKKVERIQKIMQEAAEQSYRAVVPTCTYISPKELQQKI
DGYDAVVIAYEESAKEGEQSAFAQLLTQLKPKQRVLVITGPEGGFDVNEINQWTQAGATACAFGPRILRSETAPLYALSA
ISFALELNRSYENG

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=227, Percent_Identity=33.4801762114537, Blast_Score=115, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 27926; Mature: 27926

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRYFLTKEAFKGETVTLPSDVVHHIGTVLRQGPGYEMVLLDGTGVEYACVVTSLEKKSG
CCCEECCHHHCCCCEEECHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEEEHHHHCCC
EAKVMSSRQATTELPIEVTLAYALPKGDKVELVAQKATELGVHHLLLFESTRSVAKWDGK
CHHHHHCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHCHHHEEEECCCHHHHHCCCC
KAPKKVERIQKIMQEAAEQSYRAVVPTCTYISPKELQQKIDGYDAVVIAYEESAKEGEQS
CCHHHHHHHHHHHHHHHHHCCEEECCEECCCCHHHHHHHHCCCCEEEEEECCCCCCCHHH
AFAQLLTQLKPKQRVLVITGPEGGFDVNEINQWTQAGATACAFGPRILRSETAPLYALSA
HHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCCHHHHCCCHHHHCCCCCHHHHHH
ISFALELNRSYENG
HHHHHHHCCCCCCC
>Mature Secondary Structure
MQRYFLTKEAFKGETVTLPSDVVHHIGTVLRQGPGYEMVLLDGTGVEYACVVTSLEKKSG
CCCEECCHHHCCCCEEECHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEEEHHHHCCC
EAKVMSSRQATTELPIEVTLAYALPKGDKVELVAQKATELGVHHLLLFESTRSVAKWDGK
CHHHHHCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHCHHHEEEECCCHHHHHCCCC
KAPKKVERIQKIMQEAAEQSYRAVVPTCTYISPKELQQKIDGYDAVVIAYEESAKEGEQS
CCHHHHHHHHHHHHHHHHHCCEEECCEECCCCHHHHHHHHCCCCEEEEEECCCCCCCHHH
AFAQLLTQLKPKQRVLVITGPEGGFDVNEINQWTQAGATACAFGPRILRSETAPLYALSA
HHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCCHHHHCCCHHHHCCCCCHHHHHH
ISFALELNRSYENG
HHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9023197; 9384377 [H]