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The map label for this gene is sigA [H]

Identifier: 229916320

GI number: 229916320

Start: 539617

End: 540711

Strand: Reverse

Name: sigA [H]

Synonym: EAT1b_0589

Alternate gene names: 229916320

Gene position: 540711-539617 (Counterclockwise)

Preceding gene: 229916321

Following gene: 229916319

Centisome position: 18.02

GC content: 46.85

Gene sequence:

>1095_bases
ATGGCAGAGAAGAAGAAAGAGGATTTATTTAACGCCATAGAGGAACTTGAAAAGTTAGGTAAGAAAAAAGGCGTCGTCTC
AATGCAAGCAATCCAAGATCGCCTCAGTCACTTCGAGGTGGATTCGGATTTCATCGATAACTTCATCGAAGATTTAGAGG
CTAAAGACATCCGCGTCACAGAAGATGCAGCGGACGCAGAAGAAACACCTGCGAAAAAAGAGGATGAAGAAGTCGATTTG
AACGACTTATCAGTACCTCCCGGAATCAAAATCAACGACCCAGTTCGAATGTATTTGAAAGAAATCGGACGAGTCGATCT
CCTCAGTGCGAAAGATGAGACAGAGCTCGCTAAACGAATCGAGCAAGGGGACGAGGAAGCGAAGAAACGTCTCGCAGAAG
CGAACCTACGTCTCGTCGTGTCGATTGCGAAGCGTTATGTCGGTCGTGGGATGCTCTTCCTTGATTTGATTCAAGAAGGG
AACATGGGACTTATTAAAGCGGTCGAAAAATTCGACTACATGAAAGGGTTCAAATTCTCGACGTATGCGACGTGGTGGAT
TCGCCAAGCGATCACACGTGCGATTGCTGACCAAGCCCGTACGATTCGTATTCCTGTACACATGGTGGAAACAATCAACA
AATTGATTCGTGTCCAACGTCAATTGCTTCAAGACCTCGGTCGCGAACCTTCTCCGGAAGAGATTGCCAAAGAGATGGAA
CTCACACCTGAGAAGGTCCGTGAAATTCTAAAGATTGCCCAAGAACCTGTTTCTTTAGAAACACCGATTGGGGAAGAAGA
CGACTCACATCTCGGTGACTTCATCGAAGACCAAGATGCGATGGCTCCTCAAGATGCGGCAGCCTATGAATTGTTGAAAG
AGCAATTAGAAGACGTACTAGATACGTTGACAGACCGTGAAGAGAACGTCCTTCGCCTTCGTTTCGGTCTAGATGACGGC
CGCACACGCACACTCGAAGAAGTTGGTAAAGTATTCGGTGTCACACGTGAGCGGATTCGTCAAATCGAAGCGAAAGCCCT
TCGTAAATTGCGTCATCCGAGTCGTTCCAAACGCTTGAAAGACTTCCTCGATTAA

Upstream 100 bases:

>100_bases
TTGTAGACCAGGCGTCCAAGCTACAAGAATTGATTGCCCGTAGTCGTCGTCTTCGAGAACGATGATCGTGGATGAAGTTT
GGAAGGAGTTGAACTGCTAG

Downstream 100 bases:

>100_bases
TTTCATATTTGAATAAAGTGTGAAGTTTTAGAAGAAAGGAAGGAAAATGAGTGATTTTCCTTCCTTCTTTATTATTTTTT
GAGTAAAATAGTGTTGGATA

Product: RNA polymerase, sigma 70 subunit, RpoD subfamily

Products: NA

Alternate protein names: Sigma-43; Sigma-A [H]

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MAEKKKEDLFNAIEELEKLGKKKGVVSMQAIQDRLSHFEVDSDFIDNFIEDLEAKDIRVTEDAADAEETPAKKEDEEVDL
NDLSVPPGIKINDPVRMYLKEIGRVDLLSAKDETELAKRIEQGDEEAKKRLAEANLRLVVSIAKRYVGRGMLFLDLIQEG
NMGLIKAVEKFDYMKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVQRQLLQDLGREPSPEEIAKEME
LTPEKVREILKIAQEPVSLETPIGEEDDSHLGDFIEDQDAMAPQDAAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDG
RTRTLEEVGKVFGVTRERIRQIEAKALRKLRHPSRSKRLKDFLD

Sequences:

>Translated_364_residues
MAEKKKEDLFNAIEELEKLGKKKGVVSMQAIQDRLSHFEVDSDFIDNFIEDLEAKDIRVTEDAADAEETPAKKEDEEVDL
NDLSVPPGIKINDPVRMYLKEIGRVDLLSAKDETELAKRIEQGDEEAKKRLAEANLRLVVSIAKRYVGRGMLFLDLIQEG
NMGLIKAVEKFDYMKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVQRQLLQDLGREPSPEEIAKEME
LTPEKVREILKIAQEPVSLETPIGEEDDSHLGDFIEDQDAMAPQDAAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDG
RTRTLEEVGKVFGVTRERIRQIEAKALRKLRHPSRSKRLKDFLD
>Mature_363_residues
AEKKKEDLFNAIEELEKLGKKKGVVSMQAIQDRLSHFEVDSDFIDNFIEDLEAKDIRVTEDAADAEETPAKKEDEEVDLN
DLSVPPGIKINDPVRMYLKEIGRVDLLSAKDETELAKRIEQGDEEAKKRLAEANLRLVVSIAKRYVGRGMLFLDLIQEGN
MGLIKAVEKFDYMKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVQRQLLQDLGREPSPEEIAKEMEL
TPEKVREILKIAQEPVSLETPIGEEDDSHLGDFIEDQDAMAPQDAAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGR
TRTLEEVGKVFGVTRERIRQIEAKALRKLRHPSRSKRLKDFLD

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=238, Percent_Identity=68.4873949579832, Blast_Score=343, Evalue=1e-95,
Organism=Escherichia coli, GI1789098, Length=311, Percent_Identity=41.8006430868167, Blast_Score=237, Evalue=1e-63,
Organism=Escherichia coli, GI1789871, Length=272, Percent_Identity=31.6176470588235, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1788231, Length=202, Percent_Identity=28.7128712871287, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 41814; Mature: 41683

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEKKKEDLFNAIEELEKLGKKKGVVSMQAIQDRLSHFEVDSDFIDNFIEDLEAKDIRVT
CCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEC
EDAADAEETPAKKEDEEVDLNDLSVPPGIKINDPVRMYLKEIGRVDLLSAKDETELAKRI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEECCCCHHHHHHHH
EQGDEEAKKRLAEANLRLVVSIAKRYVGRGMLFLDLIQEGNMGLIKAVEKFDYMKGFKFS
HCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCH
TYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVQRQLLQDLGREPSPEEIAKEME
HHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHC
LTPEKVREILKIAQEPVSLETPIGEEDDSHLGDFIEDQDAMAPQDAAAYELLKEQLEDVL
CCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
DTLTDREENVLRLRFGLDDGRTRTLEEVGKVFGVTRERIRQIEAKALRKLRHPSRSKRLK
HHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
DFLD
HHCC
>Mature Secondary Structure 
AEKKKEDLFNAIEELEKLGKKKGVVSMQAIQDRLSHFEVDSDFIDNFIEDLEAKDIRVT
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEC
EDAADAEETPAKKEDEEVDLNDLSVPPGIKINDPVRMYLKEIGRVDLLSAKDETELAKRI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEECCCCHHHHHHHH
EQGDEEAKKRLAEANLRLVVSIAKRYVGRGMLFLDLIQEGNMGLIKAVEKFDYMKGFKFS
HCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCH
TYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVQRQLLQDLGREPSPEEIAKEME
HHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHC
LTPEKVREILKIAQEPVSLETPIGEEDDSHLGDFIEDQDAMAPQDAAAYELLKEQLEDVL
CCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
DTLTDREENVLRLRFGLDDGRTRTLEEVGKVFGVTRERIRQIEAKALRKLRHPSRSKRLK
HHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
DFLD
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]