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The map label for this gene is dnaG [H]

Identifier: 229916321

GI number: 229916321

Start: 540747

End: 542558

Strand: Reverse

Name: dnaG [H]

Synonym: EAT1b_0590

Alternate gene names: 229916321

Gene position: 542558-540747 (Counterclockwise)

Preceding gene: 229916322

Following gene: 229916320

Centisome position: 18.09

GC content: 49.56

Gene sequence:

>1812_bases
ATGCCACGAATACCAGATGAAGTCATCGCAGAAGTGAAACAAGCGACCGATATCGTAGAATTGATTGGCGAACGTGTCGA
ACTAAAGAAACAAGGACACCGCCTTGTCGGATTATGTCCGTTTCATTCCGAAAACTCACCATCTTTCTCCGTCTCACCTG
AAAAAGGGATGTATCACTGTTTCGGTTGTGGCGCCGGCGGAAATGTATTTACGTTCGTGATGGAAACCGAGGGGTTGTCG
TTTCCTGAGACGGTCGAACGCTTGGCAACACGGGCGAATATTTCGGTAGACGGCATTCAAGGAGACGAGGAATTGACGCG
TGAACAGGTATGGCGCAAACAGATGCGTGATGCGCATCGCCTTGTCGCCGACTTGTATCATGAAGTGTTGATGAAAACAG
AAGCTGGAGAAGTGGGCTTAACGTATTTACGGAATCGTGACCTAGCTGAAGAGACGATTGCGCAATACAAGATCGGTTTT
GCCCCAGACCAACGACGCTTTACTGTCGAGCTGTTGGAGCGGCGCGGGTTTGATTTAGAAGTAATGGTTGAGGCAGGTTT
GATCTCAAAGTCGAAGTCCGGTGAATATTTTGACCGATTTGAAGGACGGGTCGTCTTTCCGATTGCGGATCGAGACGGCA
CCATCGTTGGCTTCAGTGGACGAGCGATTGACGACCGACAACCCAAGTATGTGAATACGGCTGAGACGCCACTCTTCAAT
AAGAGCGAGTTGTTGTTCGGTTTCTCCCAAGCCCGAGCGACGATGCGTAAGAAAAAACGTGTCTTCCTCGTTGAAGGGAA
CGTCGACGTCCTGCAAGTCGCGCAAGCGGGCACACAAGAAGTTGTGGCATCGCTCGGGACCGCGTTCTCCCCTCAACACG
CAAGGGCGCTATCCCGCATCGTGGAAGAAGTCATCATCTGTTATGACGGAGACCACGCGGGACAAGAAGCGACACAAAAA
GTCATCCGCCTCTTAGAAGCGTCTCCACTCGATGTCCAAGTTTTGCCACTACCGGAAAAGACGGACCCAGATGATTATAT
CCGTCAGCATGGGGCAACTCACTGGCAACAGTTTATTGAATCTGAGCAGTTGAGTGTTCTCGATTTTATGAATCGCATGT
ACCGACGTGGAAAAAACTTGAACCGAGAAGGAGAACGGATTCGCTTTATTGAATCTATGCTTGAAGAGATCGGTAAGACA
AATGATTCGATTCGAAGAGATTTATATGTGAAGAAATTATCGGATGAGTTTGAGATCCCGCTAGAGACGCTCCGTTCGAG
AATCCCTCGTCCGACGTCAAGCGAATCAACTCCCACCCGCGCGATGGAACCTGTAGAGCCGGTACGAAAACGCTCGAGTA
AAGCAGGTGAGCCACGTTGGATGAAAGCCGAGAAGTACTTGTTGCGTGCGATGTTGACGGGGCCGGAGCAGGTCCGTTTC
GTACGCGACCGTTTAGGGCTTCGTTATCAAGATGAAACCCATCGTATGTTAGGTTCAGCATTGTATGAAATCGTGTCCGA
AACGATGACACAGGACGAGTTTGATCAGATCACGCTTCCGTTCATGGAGCGTTATTGCGATCAACGTCCTGATTATGCGA
TGCGCTTTGCAGAGATTCGTCTGATGGCTGGACCGGATGAACTGTCTGAAGATGTTTTAGACGAATACATTCGGGTCATA
AACAGTTATGAGGAGCAACGTCAAATCGAGCGTGCAAAAAGGGAAATCGCTCAAGACGCCGAAAGTATTGTAGACCAGGC
GTCCAAGCTACAAGAATTGATTGCCCGTAGTCGTCGTCTTCGAGAACGATGA

Upstream 100 bases:

>100_bases
ATTGCTTCAAGAGGGTATAATAGAGTATTGACAGTACAGTGAAACGGAACGTGATGAGCGTTCCGTTTCGTTGCGAGATT
GGAAACAGGGAGTGGTCAAC

Downstream 100 bases:

>100_bases
TCGTGGATGAAGTTTGGAAGGAGTTGAACTGCTAGATGGCAGAGAAGAAGAAAGAGGATTTATTTAACGCCATAGAGGAA
CTTGAAAAGTTAGGTAAGAA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 603; Mature: 602

Protein sequence:

>603_residues
MPRIPDEVIAEVKQATDIVELIGERVELKKQGHRLVGLCPFHSENSPSFSVSPEKGMYHCFGCGAGGNVFTFVMETEGLS
FPETVERLATRANISVDGIQGDEELTREQVWRKQMRDAHRLVADLYHEVLMKTEAGEVGLTYLRNRDLAEETIAQYKIGF
APDQRRFTVELLERRGFDLEVMVEAGLISKSKSGEYFDRFEGRVVFPIADRDGTIVGFSGRAIDDRQPKYVNTAETPLFN
KSELLFGFSQARATMRKKKRVFLVEGNVDVLQVAQAGTQEVVASLGTAFSPQHARALSRIVEEVIICYDGDHAGQEATQK
VIRLLEASPLDVQVLPLPEKTDPDDYIRQHGATHWQQFIESEQLSVLDFMNRMYRRGKNLNREGERIRFIESMLEEIGKT
NDSIRRDLYVKKLSDEFEIPLETLRSRIPRPTSSESTPTRAMEPVEPVRKRSSKAGEPRWMKAEKYLLRAMLTGPEQVRF
VRDRLGLRYQDETHRMLGSALYEIVSETMTQDEFDQITLPFMERYCDQRPDYAMRFAEIRLMAGPDELSEDVLDEYIRVI
NSYEEQRQIERAKREIAQDAESIVDQASKLQELIARSRRLRER

Sequences:

>Translated_603_residues
MPRIPDEVIAEVKQATDIVELIGERVELKKQGHRLVGLCPFHSENSPSFSVSPEKGMYHCFGCGAGGNVFTFVMETEGLS
FPETVERLATRANISVDGIQGDEELTREQVWRKQMRDAHRLVADLYHEVLMKTEAGEVGLTYLRNRDLAEETIAQYKIGF
APDQRRFTVELLERRGFDLEVMVEAGLISKSKSGEYFDRFEGRVVFPIADRDGTIVGFSGRAIDDRQPKYVNTAETPLFN
KSELLFGFSQARATMRKKKRVFLVEGNVDVLQVAQAGTQEVVASLGTAFSPQHARALSRIVEEVIICYDGDHAGQEATQK
VIRLLEASPLDVQVLPLPEKTDPDDYIRQHGATHWQQFIESEQLSVLDFMNRMYRRGKNLNREGERIRFIESMLEEIGKT
NDSIRRDLYVKKLSDEFEIPLETLRSRIPRPTSSESTPTRAMEPVEPVRKRSSKAGEPRWMKAEKYLLRAMLTGPEQVRF
VRDRLGLRYQDETHRMLGSALYEIVSETMTQDEFDQITLPFMERYCDQRPDYAMRFAEIRLMAGPDELSEDVLDEYIRVI
NSYEEQRQIERAKREIAQDAESIVDQASKLQELIARSRRLRER
>Mature_602_residues
PRIPDEVIAEVKQATDIVELIGERVELKKQGHRLVGLCPFHSENSPSFSVSPEKGMYHCFGCGAGGNVFTFVMETEGLSF
PETVERLATRANISVDGIQGDEELTREQVWRKQMRDAHRLVADLYHEVLMKTEAGEVGLTYLRNRDLAEETIAQYKIGFA
PDQRRFTVELLERRGFDLEVMVEAGLISKSKSGEYFDRFEGRVVFPIADRDGTIVGFSGRAIDDRQPKYVNTAETPLFNK
SELLFGFSQARATMRKKKRVFLVEGNVDVLQVAQAGTQEVVASLGTAFSPQHARALSRIVEEVIICYDGDHAGQEATQKV
IRLLEASPLDVQVLPLPEKTDPDDYIRQHGATHWQQFIESEQLSVLDFMNRMYRRGKNLNREGERIRFIESMLEEIGKTN
DSIRRDLYVKKLSDEFEIPLETLRSRIPRPTSSESTPTRAMEPVEPVRKRSSKAGEPRWMKAEKYLLRAMLTGPEQVRFV
RDRLGLRYQDETHRMLGSALYEIVSETMTQDEFDQITLPFMERYCDQRPDYAMRFAEIRLMAGPDELSEDVLDEYIRVIN
SYEEQRQIERAKREIAQDAESIVDQASKLQELIARSRRLRER

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=357, Percent_Identity=38.0952380952381, Blast_Score=245, Evalue=5e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007693
- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 69335; Mature: 69203

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRIPDEVIAEVKQATDIVELIGERVELKKQGHRLVGLCPFHSENSPSFSVSPEKGMYHC
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCEEE
FGCGAGGNVFTFVMETEGLSFPETVERLATRANISVDGIQGDEELTREQVWRKQMRDAHR
EECCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHHHHHH
LVADLYHEVLMKTEAGEVGLTYLRNRDLAEETIAQYKIGFAPDQRRFTVELLERRGFDLE
HHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEE
VMVEAGLISKSKSGEYFDRFEGRVVFPIADRDGTIVGFSGRAIDDRQPKYVNTAETPLFN
EEEECCHHCCCCCCCHHHHHCCEEEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCC
KSELLFGFSQARATMRKKKRVFLVEGNVDVLQVAQAGTQEVVASLGTAFSPQHARALSRI
CHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHH
VEEVIICYDGDHAGQEATQKVIRLLEASPLDVQVLPLPEKTDPDDYIRQHGATHWQQFIE
HHHHHEEECCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHCCCHHHHHHHH
SEQLSVLDFMNRMYRRGKNLNREGERIRFIESMLEEIGKTNDSIRRDLYVKKLSDEFEIP
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCC
LETLRSRIPRPTSSESTPTRAMEPVEPVRKRSSKAGEPRWMKAEKYLLRAMLTGPEQVRF
HHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHH
VRDRLGLRYQDETHRMLGSALYEIVSETMTQDEFDQITLPFMERYCDQRPDYAMRFAEIR
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHCCHHHHHHHHCCCCCHHHHHHHHE
LMAGPDELSEDVLDEYIRVINSYEEQRQIERAKREIAQDAESIVDQASKLQELIARSRRL
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RER
CCC
>Mature Secondary Structure 
PRIPDEVIAEVKQATDIVELIGERVELKKQGHRLVGLCPFHSENSPSFSVSPEKGMYHC
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCEEE
FGCGAGGNVFTFVMETEGLSFPETVERLATRANISVDGIQGDEELTREQVWRKQMRDAHR
EECCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHHHHHH
LVADLYHEVLMKTEAGEVGLTYLRNRDLAEETIAQYKIGFAPDQRRFTVELLERRGFDLE
HHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEE
VMVEAGLISKSKSGEYFDRFEGRVVFPIADRDGTIVGFSGRAIDDRQPKYVNTAETPLFN
EEEECCHHCCCCCCCHHHHHCCEEEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCC
KSELLFGFSQARATMRKKKRVFLVEGNVDVLQVAQAGTQEVVASLGTAFSPQHARALSRI
CHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHH
VEEVIICYDGDHAGQEATQKVIRLLEASPLDVQVLPLPEKTDPDDYIRQHGATHWQQFIE
HHHHHEEECCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHCCCHHHHHHHH
SEQLSVLDFMNRMYRRGKNLNREGERIRFIESMLEEIGKTNDSIRRDLYVKKLSDEFEIP
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCC
LETLRSRIPRPTSSESTPTRAMEPVEPVRKRSSKAGEPRWMKAEKYLLRAMLTGPEQVRF
HHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHH
VRDRLGLRYQDETHRMLGSALYEIVSETMTQDEFDQITLPFMERYCDQRPDYAMRFAEIR
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHCCHHHHHHHHCCCCCHHHHHHHHE
LMAGPDELSEDVLDEYIRVINSYEEQRQIERAKREIAQDAESIVDQASKLQELIARSRRL
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RER
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]