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The map label for this gene is rbsB [H]

Identifier: 229916314

GI number: 229916314

Start: 533407

End: 534345

Strand: Reverse

Name: rbsB [H]

Synonym: EAT1b_0583

Alternate gene names: 229916314

Gene position: 534345-533407 (Counterclockwise)

Preceding gene: 229916315

Following gene: 229916313

Centisome position: 17.81

GC content: 45.05

Gene sequence:

>939_bases
ATGAAAGGACTAAAACATTATTGGCTTTGGTTACCGATCGTGTGGGCCATGACGATTCTCCTTATCACCTCGATTCAACT
CCCAGAGCAAAAGACGAATGAAAAAGAAGTCGGATTCGTATTTGTCAGTGATAAACACGAACATGCGAATAAACTACTGG
ACTCGTTCGTGCATACAGCAAATCAAAAAGGGTTCATCCCGATTCCGACAACGAGTGAAGACTCACGTGTCCTTGAAAAA
GAAAAATTAGATTCATTGATTGACCGGGGTGTCGAGGCCATCTTTTTGACGACTCTCGATGACACGTTCATTCAACCTTC
ATTAGAACGCGCAAAACGTAAAAACATCCCGATTATTGCGATTGACCGCATGATTGATCATCCGAGTGTGCTCACATCCG
TCGTATCCGACAATGTTGACATCGGTCGGATGGCGGCCGAACAAATTGCTGCCAAGTATAATGAGCTCGATCGTCCGATT
CGCGTGGTCGAAGTGAGGGGAACCCCGAAAGTTCGTTCCACGATGGACCGGAGTCTCGGCATTCGAGAGTACGAGAAGCA
ACATGGTGACGATTTTCAGATCATCGCATCCATCACCGGGAACTATGACACATTCCAAGCTGAAATTGAGATGTCACGTT
GGTTAGAAGAAGAGATTCCGTTTGATGCAGTCTTTTCACATAACGATGATAATACGTTCGGTGTCGTCCGGGCTTTAGAA
AGTCACGGTTTGACGGATAAGACGATCGTGTCGGTCGACGGGATTTCAGATATCTATCCGCTCATTCAAAACGGGAAGGT
CAATGCCACTGTCGTGCAGTCACCGAACGAGATGATGGAAGTCGGCTTCAAAGCACTCGAACAACATTTAAATGGAAAGA
AAATTGCCAAACACTATTATTCATCGAGTTATTTATATGAAGGTGTGGCAAAAGATTGA

Upstream 100 bases:

>100_bases
TTCAAGAGTACTTGAAGAGCGTTAACGGTGCGATGGAGATTGATAGTGTGATGAACAAAGGGACCGCGATTTTAATCAAA
ATCCCAAGGAGACAACCGTC

Downstream 100 bases:

>100_bases
TTGACAAATCATTTGATTCCGCTATGCTAGGAAAAGTGGAAAGAAACAGGAGGTGTATCGATTGAACAGCTTCCAACAGT
TCAAATTGCTACCGTTTATG

Product: LacI family transcriptional regulator

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MKGLKHYWLWLPIVWAMTILLITSIQLPEQKTNEKEVGFVFVSDKHEHANKLLDSFVHTANQKGFIPIPTTSEDSRVLEK
EKLDSLIDRGVEAIFLTTLDDTFIQPSLERAKRKNIPIIAIDRMIDHPSVLTSVVSDNVDIGRMAAEQIAAKYNELDRPI
RVVEVRGTPKVRSTMDRSLGIREYEKQHGDDFQIIASITGNYDTFQAEIEMSRWLEEEIPFDAVFSHNDDNTFGVVRALE
SHGLTDKTIVSVDGISDIYPLIQNGKVNATVVQSPNEMMEVGFKALEQHLNGKKIAKHYYSSSYLYEGVAKD

Sequences:

>Translated_312_residues
MKGLKHYWLWLPIVWAMTILLITSIQLPEQKTNEKEVGFVFVSDKHEHANKLLDSFVHTANQKGFIPIPTTSEDSRVLEK
EKLDSLIDRGVEAIFLTTLDDTFIQPSLERAKRKNIPIIAIDRMIDHPSVLTSVVSDNVDIGRMAAEQIAAKYNELDRPI
RVVEVRGTPKVRSTMDRSLGIREYEKQHGDDFQIIASITGNYDTFQAEIEMSRWLEEEIPFDAVFSHNDDNTFGVVRALE
SHGLTDKTIVSVDGISDIYPLIQNGKVNATVVQSPNEMMEVGFKALEQHLNGKKIAKHYYSSSYLYEGVAKD
>Mature_312_residues
MKGLKHYWLWLPIVWAMTILLITSIQLPEQKTNEKEVGFVFVSDKHEHANKLLDSFVHTANQKGFIPIPTTSEDSRVLEK
EKLDSLIDRGVEAIFLTTLDDTFIQPSLERAKRKNIPIIAIDRMIDHPSVLTSVVSDNVDIGRMAAEQIAAKYNELDRPI
RVVEVRGTPKVRSTMDRSLGIREYEKQHGDDFQIIASITGNYDTFQAEIEMSRWLEEEIPFDAVFSHNDDNTFGVVRALE
SHGLTDKTIVSVDGISDIYPLIQNGKVNATVVQSPNEMMEVGFKALEQHLNGKKIAKHYYSSSYLYEGVAKD

Specific function: Involved in the high-affinity D-ribose membrane transport system [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=248, Percent_Identity=26.6129032258064, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1790674, Length=232, Percent_Identity=31.0344827586207, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788473, Length=239, Percent_Identity=21.3389121338912, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 35392; Mature: 35392

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGLKHYWLWLPIVWAMTILLITSIQLPEQKTNEKEVGFVFVSDKHEHANKLLDSFVHTA
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHC
NQKGFIPIPTTSEDSRVLEKEKLDSLIDRGVEAIFLTTLDDTFIQPSLERAKRKNIPIIA
CCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCHHHCCHHHHHHHHCCCCEEE
IDRMIDHPSVLTSVVSDNVDIGRMAAEQIAAKYNELDRPIRVVEVRGTPKVRSTMDRSLG
ECCHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCC
IREYEKQHGDDFQIIASITGNYDTFQAEIEMSRWLEEEIPFDAVFSHNDDNTFGVVRALE
HHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHH
SHGLTDKTIVSVDGISDIYPLIQNGKVNATVVQSPNEMMEVGFKALEQHLNGKKIAKHYY
HCCCCCCEEEEECCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHH
SSSYLYEGVAKD
CCHHHHCCCCCC
>Mature Secondary Structure
MKGLKHYWLWLPIVWAMTILLITSIQLPEQKTNEKEVGFVFVSDKHEHANKLLDSFVHTA
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHC
NQKGFIPIPTTSEDSRVLEKEKLDSLIDRGVEAIFLTTLDDTFIQPSLERAKRKNIPIIA
CCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCHHHCCHHHHHHHHCCCCEEE
IDRMIDHPSVLTSVVSDNVDIGRMAAEQIAAKYNELDRPIRVVEVRGTPKVRSTMDRSLG
ECCHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCC
IREYEKQHGDDFQIIASITGNYDTFQAEIEMSRWLEEEIPFDAVFSHNDDNTFGVVRALE
HHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHH
SHGLTDKTIVSVDGISDIYPLIQNGKVNATVVQSPNEMMEVGFKALEQHLNGKKIAKHYY
HCCCCCCEEEEECCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHH
SSSYLYEGVAKD
CCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 7921236 [H]