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The map label for this gene is kinA [H]

Identifier: 229916315

GI number: 229916315

Start: 534342

End: 536105

Strand: Reverse

Name: kinA [H]

Synonym: EAT1b_0584

Alternate gene names: 229916315

Gene position: 536105-534342 (Counterclockwise)

Preceding gene: 229916317

Following gene: 229916314

Centisome position: 17.87

GC content: 44.67

Gene sequence:

>1764_bases
GTGAATCGCATGAAACCATTGCGTGAACTAAGAAAATCGTACTTTTTCCAAATTACGTTCATCATCATCATTATTTTAGC
ACTTGTCATTTCATCACTTGCCTTCTCTGCTGACCGCGTCACGGTGTCTCGAGCAGAAGAATTGGCGAAAGAGCAATTTG
ATTACGCCATCGATGACAGTATTGAGAATGTGGAGTCCCGACTTGGGAAATTGTATGAGGATTTAGTTTACTTAGGCGCT
TACGTTGGAAATGAGCCATGGATTGAAGAACGGATCCGAACAGGATATTTGAATCAACGCACGAGTTTTACGTCCATCTT
TTATTATGATGTCAATCAAGACTCATTTAAATGGTACTCGGAGCGACAAGTCGCCTCTTCTGTTCTGAAACGGGATCCGA
AGTTCACGGAAGTATTGACTGCAAGTGGAAAAGAACTATATATGAGTGACCCTGTGATGATCAGTGGGGATATGGGAACT
TTCCTATACGTTCCGGTCATCGAAGAAAATCGACCAATCGGTATTTTTGGGGGAAGCATTAGTGTCTTGAACTCTGACTT
GTATCGTCGTTCGTTGGAATCTGCGAATGATGACATGATTCTATACTTGTTAGATGAGCAAGGCGAAGTGTTGACGACTT
CGTTCAACTTGGTCACAGATGCAGAGCGTCGGATATCGCGTGAAGTCATCGACGAAGCATTAAATCGAAGCGAACGGACA
ATCTTGAGTACGGATGAAGATACGGTAATGTTTGAACCGGATGCACGAGTCAGTGGATGGAGCATGCTCGCAAAACATCA
AAATGACTCCGTCTATGAAACGGTCTACGACACGCGTTGGCAGTATTTGATGATTGCGCTCGTTACATTCTTCCTGAGCA
TTCTCGTTGGATTGTTGCTTTCCCGGACGTTGACGAATCCGCTTGTCGAGCTGGTCAACCAGATTAAACAACAACAATCG
CTCGGAACGATTTCATTGGAACGAACGGGTTCGAAAGAAGTGGAGACGCTACTCGATACGTATAACGATTATGCACAGCG
GATGGAAACGTCACGACGTGAACAGTTGAGTCAGCAACAGTTGATTTTGCATCAAGAAAAATTAGCCTCGCTCGGTCAAT
TGGCCGCCGGGATTGCCCATGAAATTCGAAATCCTCTGACTCCCGTCCATATCACGTTGCAGATGGTGCGCGAGGGTCAA
GGTAGTTCAGATATGGTGGATGTCGCCATCAAAGAACTGGAGCGGGCGAATGATTTGATTTCAACGATGCTAACGCTCGC
CAAACCCGATCAGGCTGGTAAAAATGAAGATTGGATCAACATGTTAGACTTTGCGAAACGACTTGAATTTTTAATGGATG
CCGAATGTTACAAACGACCGACAAAATGGGAATTACTTGTACCGAACGACATGCCTCCATTTTATGCATCCTTTGATATG
CTCGTTCAAATTATCTACAACCTCTTCAAAAATGCGGTCGATGCCGTCGAATCAAAAGGCGCTGACGGGAGTGTCGTCGT
GACGGTACTCTTTACGGAGCAGGAATATCGCTTTTTGATTGAAGATAACGGAATTGGCATGAGTGAGGAACAACTCAAGA
CAGTCGGTTCCGCCTTCAATACGACGAAGGAAAGTGGAAACGGTCTAGGAATCTATATGATTCAAGAGTACTTGAAGAGC
GTTAACGGTGCGATGGAGATTGATAGTGTGATGAACAAAGGGACCGCGATTTTAATCAAAATCCCAAGGAGACAACCGTC
ATGA

Upstream 100 bases:

>100_bases
CGAAGTGCTTTCATACGTATTAGTCTAACATTGTTTCGAAAATACGAAAAGATTTGCGTGTATTCATGCTATAATTTTTA
GGTTAGAGAGGAGGGACGCC

Downstream 100 bases:

>100_bases
AAGGACTAAAACATTATTGGCTTTGGTTACCGATCGTGTGGGCCATGACGATTCTCCTTATCACCTCGATTCAACTCCCA
GAGCAAAAGACGAATGAAAA

Product: histidine kinase

Products: NA

Alternate protein names: Stage II sporulation protein F; Stage II sporulation protein J [H]

Number of amino acids: Translated: 587; Mature: 587

Protein sequence:

>587_residues
MNRMKPLRELRKSYFFQITFIIIIILALVISSLAFSADRVTVSRAEELAKEQFDYAIDDSIENVESRLGKLYEDLVYLGA
YVGNEPWIEERIRTGYLNQRTSFTSIFYYDVNQDSFKWYSERQVASSVLKRDPKFTEVLTASGKELYMSDPVMISGDMGT
FLYVPVIEENRPIGIFGGSISVLNSDLYRRSLESANDDMILYLLDEQGEVLTTSFNLVTDAERRISREVIDEALNRSERT
ILSTDEDTVMFEPDARVSGWSMLAKHQNDSVYETVYDTRWQYLMIALVTFFLSILVGLLLSRTLTNPLVELVNQIKQQQS
LGTISLERTGSKEVETLLDTYNDYAQRMETSRREQLSQQQLILHQEKLASLGQLAAGIAHEIRNPLTPVHITLQMVREGQ
GSSDMVDVAIKELERANDLISTMLTLAKPDQAGKNEDWINMLDFAKRLEFLMDAECYKRPTKWELLVPNDMPPFYASFDM
LVQIIYNLFKNAVDAVESKGADGSVVVTVLFTEQEYRFLIEDNGIGMSEEQLKTVGSAFNTTKESGNGLGIYMIQEYLKS
VNGAMEIDSVMNKGTAILIKIPRRQPS

Sequences:

>Translated_587_residues
MNRMKPLRELRKSYFFQITFIIIIILALVISSLAFSADRVTVSRAEELAKEQFDYAIDDSIENVESRLGKLYEDLVYLGA
YVGNEPWIEERIRTGYLNQRTSFTSIFYYDVNQDSFKWYSERQVASSVLKRDPKFTEVLTASGKELYMSDPVMISGDMGT
FLYVPVIEENRPIGIFGGSISVLNSDLYRRSLESANDDMILYLLDEQGEVLTTSFNLVTDAERRISREVIDEALNRSERT
ILSTDEDTVMFEPDARVSGWSMLAKHQNDSVYETVYDTRWQYLMIALVTFFLSILVGLLLSRTLTNPLVELVNQIKQQQS
LGTISLERTGSKEVETLLDTYNDYAQRMETSRREQLSQQQLILHQEKLASLGQLAAGIAHEIRNPLTPVHITLQMVREGQ
GSSDMVDVAIKELERANDLISTMLTLAKPDQAGKNEDWINMLDFAKRLEFLMDAECYKRPTKWELLVPNDMPPFYASFDM
LVQIIYNLFKNAVDAVESKGADGSVVVTVLFTEQEYRFLIEDNGIGMSEEQLKTVGSAFNTTKESGNGLGIYMIQEYLKS
VNGAMEIDSVMNKGTAILIKIPRRQPS
>Mature_587_residues
MNRMKPLRELRKSYFFQITFIIIIILALVISSLAFSADRVTVSRAEELAKEQFDYAIDDSIENVESRLGKLYEDLVYLGA
YVGNEPWIEERIRTGYLNQRTSFTSIFYYDVNQDSFKWYSERQVASSVLKRDPKFTEVLTASGKELYMSDPVMISGDMGT
FLYVPVIEENRPIGIFGGSISVLNSDLYRRSLESANDDMILYLLDEQGEVLTTSFNLVTDAERRISREVIDEALNRSERT
ILSTDEDTVMFEPDARVSGWSMLAKHQNDSVYETVYDTRWQYLMIALVTFFLSILVGLLLSRTLTNPLVELVNQIKQQQS
LGTISLERTGSKEVETLLDTYNDYAQRMETSRREQLSQQQLILHQEKLASLGQLAAGIAHEIRNPLTPVHITLQMVREGQ
GSSDMVDVAIKELERANDLISTMLTLAKPDQAGKNEDWINMLDFAKRLEFLMDAECYKRPTKWELLVPNDMPPFYASFDM
LVQIIYNLFKNAVDAVESKGADGSVVVTVLFTEQEYRFLIEDNGIGMSEEQLKTVGSAFNTTKESGNGLGIYMIQEYLKS
VNGAMEIDSVMNKGTAILIKIPRRQPS

Specific function: Phosphorylates the sporulation-regulatory proteins spo0A and spo0F. It also autophosphorylates in the presence of ATP [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=271, Percent_Identity=29.1512915129151, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1790436, Length=243, Percent_Identity=29.2181069958848, Blast_Score=102, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR013655
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS; PF08447 PAS_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 66854; Mature: 66854

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRMKPLRELRKSYFFQITFIIIIILALVISSLAFSADRVTVSRAEELAKEQFDYAIDDS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCHHHHH
IENVESRLGKLYEDLVYLGAYVGNEPWIEERIRTGYLNQRTSFTSIFYYDVNQDSFKWYS
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCEEEEEEEECCCCHHHHHH
ERQVASSVLKRDPKFTEVLTASGKELYMSDPVMISGDMGTFLYVPVIEENRPIGIFGGSI
HHHHHHHHHHCCCCHHHHHHCCCCEEEECCCEEEECCCCCEEEEEEECCCCCEEEECCCH
SVLNSDLYRRSLESANDDMILYLLDEQGEVLTTSFNLVTDAERRISREVIDEALNRSERT
HHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCE
ILSTDEDTVMFEPDARVSGWSMLAKHQNDSVYETVYDTRWQYLMIALVTFFLSILVGLLL
EEECCCCEEEECCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRTLTNPLVELVNQIKQQQSLGTISLERTGSKEVETLLDTYNDYAQRMETSRREQLSQQQ
HHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LILHQEKLASLGQLAAGIAHEIRNPLTPVHITLQMVREGQGSSDMVDVAIKELERANDLI
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
STMLTLAKPDQAGKNEDWINMLDFAKRLEFLMDAECYKRPTKWELLVPNDMPPFYASFDM
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHCCCCCEEEECCCCCCCHHHHHHH
LVQIIYNLFKNAVDAVESKGADGSVVVTVLFTEQEYRFLIEDNGIGMSEEQLKTVGSAFN
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHH
TTKESGNGLGIYMIQEYLKSVNGAMEIDSVMNKGTAILIKIPRRQPS
CCCCCCCCEEHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEECCCCCC
>Mature Secondary Structure
MNRMKPLRELRKSYFFQITFIIIIILALVISSLAFSADRVTVSRAEELAKEQFDYAIDDS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCHHHHH
IENVESRLGKLYEDLVYLGAYVGNEPWIEERIRTGYLNQRTSFTSIFYYDVNQDSFKWYS
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCEEEEEEEECCCCHHHHHH
ERQVASSVLKRDPKFTEVLTASGKELYMSDPVMISGDMGTFLYVPVIEENRPIGIFGGSI
HHHHHHHHHHCCCCHHHHHHCCCCEEEECCCEEEECCCCCEEEEEEECCCCCEEEECCCH
SVLNSDLYRRSLESANDDMILYLLDEQGEVLTTSFNLVTDAERRISREVIDEALNRSERT
HHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCE
ILSTDEDTVMFEPDARVSGWSMLAKHQNDSVYETVYDTRWQYLMIALVTFFLSILVGLLL
EEECCCCEEEECCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRTLTNPLVELVNQIKQQQSLGTISLERTGSKEVETLLDTYNDYAQRMETSRREQLSQQQ
HHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LILHQEKLASLGQLAAGIAHEIRNPLTPVHITLQMVREGQGSSDMVDVAIKELERANDLI
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
STMLTLAKPDQAGKNEDWINMLDFAKRLEFLMDAECYKRPTKWELLVPNDMPPFYASFDM
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHCCCCCEEEECCCCCCCHHHHHHH
LVQIIYNLFKNAVDAVESKGADGSVVVTVLFTEQEYRFLIEDNGIGMSEEQLKTVGSAFN
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHH
TTKESGNGLGIYMIQEYLKSVNGAMEIDSVMNKGTAILIKIPRRQPS
CCCCCCCCEEHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2104615; 2509430; 9384377 [H]