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The map label for this gene is recN [H]

Identifier: 229916246

GI number: 229916246

Start: 469444

End: 471141

Strand: Reverse

Name: recN [H]

Synonym: EAT1b_0515

Alternate gene names: 229916246

Gene position: 471141-469444 (Counterclockwise)

Preceding gene: 229916247

Following gene: 229916245

Centisome position: 15.71

GC content: 47.06

Gene sequence:

>1698_bases
ATGTTAGCAGAACTATCAATCAAACAGTTTGCGATTATTGACGAATTAAACATTCCGTTTAATCGAGGTATGACTGTGCT
CACGGGTGAGACAGGTGCCGGTAAATCGATCTTGTTGGATGCGATTGGATTGCTTGTTGGAGGACGTGGCTCAAGTGAGT
TCGTGCGATATGGTCAAGATAAAGCTGAAATCGAAGGTCTGTTCATGATTGAACCGGATCACCCGGTCATCGAAGCGGCC
GAACAATATGGCATCGATGTCGAGGATGGGACAGTCATCTTACGTCGAGACCTACATAGTAGCGGGAAGAGCGTCTGCCG
TGTGAACGGAAAAATGATGCCGCTCTCGACGTTACGGGAGTTCGGGCGGCTCCTTGTCGATATTCACGGTCAACATGAAC
ATCAACATTTAATGGATGCAGAATTTCACCTTGGCATATTGGACCATTTCGCCGAAAAAGAAATCAGTCCGTTAGTAGAG
GAATATCAAACAGCATATGCTGCCTGGAAACAAGTCGCAGATGAATTGAAACGTCTCAGTCAAAGTGAGCAGGAGCTTGC
TCAACGAATGGATCTTTTGTCGTTTCAAACGAAAGAAATCGAGGCGGCTGAATTAAAAGTTGGTGAAGAACAGGCGTTGA
CAGAAGAGCGAGATGAATTGGCCAACTTTGAACGTATTCATCAACACATTCGTCTCTCCTATGAGGCGGTTGCCGAGGAG
TCAAATGGACTCGATCAAATTGGAGTGGCAATGCGTGAGATGGAAGAAGCGTCACAGCTCTCATCGTCTTTGTCGTCTGT
ATCGGAAACCATCTCTAACGCTTATTATTTGTTAGAAGATGCCAAGTTCCAACTGCGCGACCAGTTGGATTCGCTCGAAT
TCGACCCGATCCGATTGGATGAAATCGAGTCAAGATTGGCTCTTTTCCAGCAACTGAAACGAAAGTATGGGACGACGATC
GAAGAAGTCATCACCTATGGCGAACAAGTATCGGATGAATTGAAGTCGATGACGAACCGGGAAGAGCATTTACAGACGCT
TTCAGATGAGCTCATACGATTAGAGCAGATTGCAAGAGATGCTGGTATGAGACTCTCTTCAGCGCGTAAGGTGGCTGCGG
AGTCGCTTCAGCATGCGATTCATCGAGAACTGAAAGAACTATACATGGAAAAAACAGCGTTCGAGGTCCGTTTCAAAACC
ACATCGCTTAAACAAGATGGTTTAGATGACGTGGAGTTTTACATGATGACGAACGTTGGAGAACCGTTCAAGCCGCTCGC
AAAAGTCGCTTCAGGAGGTGAGCTTTCCCGTGTCATGCTCGCACTCAAATCCATCTTTTCAAGAACGGTCGGCGTGGCCT
CCATTATTTTTGACGAGGTCGACACAGGAGTCTCTGGACGTGTCGCGCAGGCGATGGGTGAAAAAATCTTCCGACTTTCT
GTCGGCTCTCAAGTCCTCTGTATCACGCACTTGGCACAAGTTGCTGCGATGGCAGACCAACATCTTTACATTACGAAAAC
CGAAACGAACGAGCGGACGAAAACGAATGTGACGTCCCTCGACCGCGAAAGCCGAATCGACGAATTGGGTCGCATGATTG
CTGGCGCTCAAATGACAGACTTGACGAAGCAACACGTCGAAGAATTACTTGAGATGTCACAGCAAGTAAAACAGAAGTTC
ATCAAAGGAGTGTCATAA

Upstream 100 bases:

>100_bases
GTGTGTGGAGATGATACGATTCTAATGATTGCACGCGACGAAGAGAAAGCGAAGCAAATCATCGATCGACTACTCGGAAT
GTTATGAGGTGAACGTACGT

Downstream 100 bases:

>100_bases
GTGATTACATTATGTATTGCGGACGACAATCGAGAAATCGTCGATTTGTTGAGCCGACAACTAGAATCTGAGCCTGATCT
TCGTGTCGTAGGTACGGCAT

Product: DNA repair protein RecN

Products: NA

Alternate protein names: Recombination protein N [H]

Number of amino acids: Translated: 565; Mature: 565

Protein sequence:

>565_residues
MLAELSIKQFAIIDELNIPFNRGMTVLTGETGAGKSILLDAIGLLVGGRGSSEFVRYGQDKAEIEGLFMIEPDHPVIEAA
EQYGIDVEDGTVILRRDLHSSGKSVCRVNGKMMPLSTLREFGRLLVDIHGQHEHQHLMDAEFHLGILDHFAEKEISPLVE
EYQTAYAAWKQVADELKRLSQSEQELAQRMDLLSFQTKEIEAAELKVGEEQALTEERDELANFERIHQHIRLSYEAVAEE
SNGLDQIGVAMREMEEASQLSSSLSSVSETISNAYYLLEDAKFQLRDQLDSLEFDPIRLDEIESRLALFQQLKRKYGTTI
EEVITYGEQVSDELKSMTNREEHLQTLSDELIRLEQIARDAGMRLSSARKVAAESLQHAIHRELKELYMEKTAFEVRFKT
TSLKQDGLDDVEFYMMTNVGEPFKPLAKVASGGELSRVMLALKSIFSRTVGVASIIFDEVDTGVSGRVAQAMGEKIFRLS
VGSQVLCITHLAQVAAMADQHLYITKTETNERTKTNVTSLDRESRIDELGRMIAGAQMTDLTKQHVEELLEMSQQVKQKF
IKGVS

Sequences:

>Translated_565_residues
MLAELSIKQFAIIDELNIPFNRGMTVLTGETGAGKSILLDAIGLLVGGRGSSEFVRYGQDKAEIEGLFMIEPDHPVIEAA
EQYGIDVEDGTVILRRDLHSSGKSVCRVNGKMMPLSTLREFGRLLVDIHGQHEHQHLMDAEFHLGILDHFAEKEISPLVE
EYQTAYAAWKQVADELKRLSQSEQELAQRMDLLSFQTKEIEAAELKVGEEQALTEERDELANFERIHQHIRLSYEAVAEE
SNGLDQIGVAMREMEEASQLSSSLSSVSETISNAYYLLEDAKFQLRDQLDSLEFDPIRLDEIESRLALFQQLKRKYGTTI
EEVITYGEQVSDELKSMTNREEHLQTLSDELIRLEQIARDAGMRLSSARKVAAESLQHAIHRELKELYMEKTAFEVRFKT
TSLKQDGLDDVEFYMMTNVGEPFKPLAKVASGGELSRVMLALKSIFSRTVGVASIIFDEVDTGVSGRVAQAMGEKIFRLS
VGSQVLCITHLAQVAAMADQHLYITKTETNERTKTNVTSLDRESRIDELGRMIAGAQMTDLTKQHVEELLEMSQQVKQKF
IKGVS
>Mature_565_residues
MLAELSIKQFAIIDELNIPFNRGMTVLTGETGAGKSILLDAIGLLVGGRGSSEFVRYGQDKAEIEGLFMIEPDHPVIEAA
EQYGIDVEDGTVILRRDLHSSGKSVCRVNGKMMPLSTLREFGRLLVDIHGQHEHQHLMDAEFHLGILDHFAEKEISPLVE
EYQTAYAAWKQVADELKRLSQSEQELAQRMDLLSFQTKEIEAAELKVGEEQALTEERDELANFERIHQHIRLSYEAVAEE
SNGLDQIGVAMREMEEASQLSSSLSSVSETISNAYYLLEDAKFQLRDQLDSLEFDPIRLDEIESRLALFQQLKRKYGTTI
EEVITYGEQVSDELKSMTNREEHLQTLSDELIRLEQIARDAGMRLSSARKVAAESLQHAIHRELKELYMEKTAFEVRFKT
TSLKQDGLDDVEFYMMTNVGEPFKPLAKVASGGELSRVMLALKSIFSRTVGVASIIFDEVDTGVSGRVAQAMGEKIFRLS
VGSQVLCITHLAQVAAMADQHLYITKTETNERTKTNVTSLDRESRIDELGRMIAGAQMTDLTKQHVEELLEMSQQVKQKF
IKGVS

Specific function: Involved in recombinational repair of damaged DNA. Seems to be the first protein recruited to repair centers, foci that are the site of double-strand DNA break(s), followed by recO and then recF [H]

COG id: COG0497

COG function: function code L; ATPase involved in DNA repair

Gene ontology:

Cell location: Cytoplasm, nucleoid. Note=Cytoplasmically located in untreated cells. Recruited to foci following treatment with DNA damaging agents; these foci are presumably the breaks themselves. They are almost always located within nucleoids [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recN family [H]

Homologues:

Organism=Escherichia coli, GI48994901, Length=558, Percent_Identity=36.5591397849462, Blast_Score=330, Evalue=1e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004604
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 63582; Mature: 63582

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAELSIKQFAIIDELNIPFNRGMTVLTGETGAGKSILLDAIGLLVGGRGSSEFVRYGQD
CCCCCCHHHHHHHHCCCCCHHCCEEEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHCCCC
KAEIEGLFMIEPDHPVIEAAEQYGIDVEDGTVILRRDLHSSGKSVCRVNGKMMPLSTLRE
HHHCCEEEEECCCCHHHHHHHHCCCCCCCCCEEEEHHHCCCCCCEEEECCCCCCHHHHHH
FGRLLVDIHGQHEHQHLMDAEFHLGILDHFAEKEISPLVEEYQTAYAAWKQVADELKRLS
HHHHHHHHCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSEQELAQRMDLLSFQTKEIEAAELKVGEEQALTEERDELANFERIHQHIRLSYEAVAEE
HHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SNGLDQIGVAMREMEEASQLSSSLSSVSETISNAYYLLEDAKFQLRDQLDSLEFDPIRLD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
EIESRLALFQQLKRKYGTTIEEVITYGEQVSDELKSMTNREEHLQTLSDELIRLEQIARD
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
AGMRLSSARKVAAESLQHAIHRELKELYMEKTAFEVRFKTTSLKQDGLDDVEFYMMTNVG
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHHHCCC
EPFKPLAKVASGGELSRVMLALKSIFSRTVGVASIIFDEVDTGVSGRVAQAMGEKIFRLS
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
VGSQVLCITHLAQVAAMADQHLYITKTETNERTKTNVTSLDRESRIDELGRMIAGAQMTD
CCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHH
LTKQHVEELLEMSQQVKQKFIKGVS
HHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLAELSIKQFAIIDELNIPFNRGMTVLTGETGAGKSILLDAIGLLVGGRGSSEFVRYGQD
CCCCCCHHHHHHHHCCCCCHHCCEEEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHCCCC
KAEIEGLFMIEPDHPVIEAAEQYGIDVEDGTVILRRDLHSSGKSVCRVNGKMMPLSTLRE
HHHCCEEEEECCCCHHHHHHHHCCCCCCCCCEEEEHHHCCCCCCEEEECCCCCCHHHHHH
FGRLLVDIHGQHEHQHLMDAEFHLGILDHFAEKEISPLVEEYQTAYAAWKQVADELKRLS
HHHHHHHHCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSEQELAQRMDLLSFQTKEIEAAELKVGEEQALTEERDELANFERIHQHIRLSYEAVAEE
HHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SNGLDQIGVAMREMEEASQLSSSLSSVSETISNAYYLLEDAKFQLRDQLDSLEFDPIRLD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
EIESRLALFQQLKRKYGTTIEEVITYGEQVSDELKSMTNREEHLQTLSDELIRLEQIARD
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
AGMRLSSARKVAAESLQHAIHRELKELYMEKTAFEVRFKTTSLKQDGLDDVEFYMMTNVG
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHHHCCC
EPFKPLAKVASGGELSRVMLALKSIFSRTVGVASIIFDEVDTGVSGRVAQAMGEKIFRLS
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
VGSQVLCITHLAQVAAMADQHLYITKTETNERTKTNVTSLDRESRIDELGRMIAGAQMTD
CCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHH
LTKQHVEELLEMSQQVKQKFIKGVS
HHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2106508; 8969508; 9384377; 2507400; 9044256 [H]