Click here to switch to the map view.

The map label for this gene is yqiR [H]

Identifier: 229916243

GI number: 229916243

Start: 466035

End: 467672

Strand: Reverse

Name: yqiR [H]

Synonym: EAT1b_0512

Alternate gene names: 229916243

Gene position: 467672-466035 (Counterclockwise)

Preceding gene: 229916245

Following gene: 229916242

Centisome position: 15.59

GC content: 46.83

Gene sequence:

>1638_bases
ATGAATAAACGATTGATGATTGTCGGAGCTGGAAAAGGCGGGACTGAGGTATTAAATATGTTGATGGATTCACCATTGGC
GACCATTATCGCGGTCGTAGATTCTGATGCATCTGCCGAAGGAATAGCCCTTGCTAAAAGTTATGGCATATCTGTGGCGG
ACGATTGGAAACAGTATGCGGATGCGACTCTAGATTTTGTCATCGAAACGACGGGGAAGCATCAAACGTTCGAACAATTA
AAAGATCATTTCAAAGATGCAGTCGTCTTACCAGGGGAATTTATTCGAATTGTGGTCGAACGATTGAAACAGAATCAACA
AATGCTCGAGGCCATTATTGAATGTACGAGTGAGGCCATCTCGGTCGCAGATCATCACGGTAACACGATTTTGATCAATC
CGTCTTATACACGGTTGACCGGATTCAAAAAAGAAGATGTGGTCGACAAACCAGCGACGGCAGATATCGGGATGCAGGAA
AGCGTCCATTTAAAAGTACTATCGACCGGGCAAGGCGTTCGGGACATCCGGATGCAAATTGGGGCGACACGCCGAGATAT
TCTTGTGAATGCGGAACCGATCATCGTTGAAGGGAAGTTGCGAGGATCGGTCGGGGTCATTCGTGACGTATCTCAGATTC
GTGCACTGCGTGATGAATTGCAGGAGGCAAAGACACGCATTCGGAATCTAGAAGCGAAATATCAGTTTGCCGATATCATT
GCGACGAGTGATGCCATGCGATTTGTCATCGAACAGGCAAAACTTGCCGCTGTGACCCCTGTGACGGTATTGATTCGCGG
AGAATCGGGAACAGGAAAGGAACTGTTCGCCCATGCCATTCATGGAGAATCTCCTCGAAAATATAAAAAGTTCATTCGCG
TGAACTGTGCGGCGATTTCACCGACGCTACTTGAAAGTGAGCTATTCGGTTATGAAGATGGAGCCTTCAGTGGAGCTAGA
CGAGGCGGGAAAACGGGATATTTCGAAGAAGCGGATGGCGGATCACTCTTTCTAGACGAAATCGGGGAGCTGCCCCTTGA
TGTCCAAGTGAAGCTCTTACGTGTCCTCCAAGAAAATGAAATTGTCCGAGTCGGAGGGACGACAGCAATCCCAATCGATG
TCCGGATCATTGCCGCAACGAATGCCGATCTTGAACAAAAGGTAGCAGACGGAACATTCCGGGAAGATTTGTATTATCGA
ATCAATCGGATGCCGATCCATATTCCACCATTACGTGAGCGATTAAATGAACTCGAGGCACTATGTGAACGGATAATCCG
TAAACTGAATCAAGAGTACGGTCGTAGTGTACGCGGCGTTGACGAAGATGCCTTATCATTACTGAAGAGCCAACATTGGA
AAGGGAATGTGCGAGAACTTGAGAATGTGATTGGGCGAGCGATGATTTATATGGCAAGTTCCGAACAGTGGATTGCCCCA
CATCACCTGCAGTTATCCAATCGTTCCACGAGTCAAAAAGTAGTCGAATCTGGAACGTTTCACGAGGCCGTTGTAAAATA
TGAACGCGATTTGATTGAACGAACAATTGAAGAGGCAGGCGGCAACAAATCAGAAGCGGCCCGCCGATTAAAGATTTCGA
TTCGAACATTATACAATAAACTCGGAACCACGCAATAA

Upstream 100 bases:

>100_bases
GAAATCGATCTGTTCGACACGGTCTATTTTTGGGCAGCTTGCGAATCAGGTGAGGATTCAGTAGCATGAAATAGAGAAGA
GAGTTGATGGGGGAATCGGT

Downstream 100 bases:

>100_bases
TTTGCATGAATATTCCTGCATGGTAAGCATTAATTTGCATGGTCATCCGCATTATAAAGCGCTTACAATTTAGGAAACAA
ACTTGCTTATGATATAGTGT

Product: PAS modulated sigma54 specific transcriptional regulator, Fis family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 545; Mature: 545

Protein sequence:

>545_residues
MNKRLMIVGAGKGGTEVLNMLMDSPLATIIAVVDSDASAEGIALAKSYGISVADDWKQYADATLDFVIETTGKHQTFEQL
KDHFKDAVVLPGEFIRIVVERLKQNQQMLEAIIECTSEAISVADHHGNTILINPSYTRLTGFKKEDVVDKPATADIGMQE
SVHLKVLSTGQGVRDIRMQIGATRRDILVNAEPIIVEGKLRGSVGVIRDVSQIRALRDELQEAKTRIRNLEAKYQFADII
ATSDAMRFVIEQAKLAAVTPVTVLIRGESGTGKELFAHAIHGESPRKYKKFIRVNCAAISPTLLESELFGYEDGAFSGAR
RGGKTGYFEEADGGSLFLDEIGELPLDVQVKLLRVLQENEIVRVGGTTAIPIDVRIIAATNADLEQKVADGTFREDLYYR
INRMPIHIPPLRERLNELEALCERIIRKLNQEYGRSVRGVDEDALSLLKSQHWKGNVRELENVIGRAMIYMASSEQWIAP
HHLQLSNRSTSQKVVESGTFHEAVVKYERDLIERTIEEAGGNKSEAARRLKISIRTLYNKLGTTQ

Sequences:

>Translated_545_residues
MNKRLMIVGAGKGGTEVLNMLMDSPLATIIAVVDSDASAEGIALAKSYGISVADDWKQYADATLDFVIETTGKHQTFEQL
KDHFKDAVVLPGEFIRIVVERLKQNQQMLEAIIECTSEAISVADHHGNTILINPSYTRLTGFKKEDVVDKPATADIGMQE
SVHLKVLSTGQGVRDIRMQIGATRRDILVNAEPIIVEGKLRGSVGVIRDVSQIRALRDELQEAKTRIRNLEAKYQFADII
ATSDAMRFVIEQAKLAAVTPVTVLIRGESGTGKELFAHAIHGESPRKYKKFIRVNCAAISPTLLESELFGYEDGAFSGAR
RGGKTGYFEEADGGSLFLDEIGELPLDVQVKLLRVLQENEIVRVGGTTAIPIDVRIIAATNADLEQKVADGTFREDLYYR
INRMPIHIPPLRERLNELEALCERIIRKLNQEYGRSVRGVDEDALSLLKSQHWKGNVRELENVIGRAMIYMASSEQWIAP
HHLQLSNRSTSQKVVESGTFHEAVVKYERDLIERTIEEAGGNKSEAARRLKISIRTLYNKLGTTQ
>Mature_545_residues
MNKRLMIVGAGKGGTEVLNMLMDSPLATIIAVVDSDASAEGIALAKSYGISVADDWKQYADATLDFVIETTGKHQTFEQL
KDHFKDAVVLPGEFIRIVVERLKQNQQMLEAIIECTSEAISVADHHGNTILINPSYTRLTGFKKEDVVDKPATADIGMQE
SVHLKVLSTGQGVRDIRMQIGATRRDILVNAEPIIVEGKLRGSVGVIRDVSQIRALRDELQEAKTRIRNLEAKYQFADII
ATSDAMRFVIEQAKLAAVTPVTVLIRGESGTGKELFAHAIHGESPRKYKKFIRVNCAAISPTLLESELFGYEDGAFSGAR
RGGKTGYFEEADGGSLFLDEIGELPLDVQVKLLRVLQENEIVRVGGTTAIPIDVRIIAATNADLEQKVADGTFREDLYYR
INRMPIHIPPLRERLNELEALCERIIRKLNQEYGRSVRGVDEDALSLLKSQHWKGNVRELENVIGRAMIYMASSEQWIAP
HHLQLSNRSTSQKVVESGTFHEAVVKYERDLIERTIEEAGGNKSEAARRLKISIRTLYNKLGTTQ

Specific function: Member Of The Two-Component Regulatory System Atos/Atoc Involved In The Transcriptional Regulation Of The Ato Genes For Acetoacetate Metabolism. Also An Inhibitor Of Polyamine Biosynthesis. [C]

COG id: COG3829

COG function: function code KT; Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=337, Percent_Identity=43.026706231454, Blast_Score=250, Evalue=1e-67,
Organism=Escherichia coli, GI87082117, Length=255, Percent_Identity=49.8039215686275, Blast_Score=248, Evalue=8e-67,
Organism=Escherichia coli, GI1790437, Length=307, Percent_Identity=43.6482084690554, Blast_Score=247, Evalue=1e-66,
Organism=Escherichia coli, GI1789087, Length=235, Percent_Identity=51.063829787234, Blast_Score=239, Evalue=4e-64,
Organism=Escherichia coli, GI1790299, Length=337, Percent_Identity=41.839762611276, Blast_Score=231, Evalue=7e-62,
Organism=Escherichia coli, GI1786524, Length=326, Percent_Identity=38.9570552147239, Blast_Score=228, Evalue=7e-61,
Organism=Escherichia coli, GI1789233, Length=297, Percent_Identity=42.4242424242424, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI1788905, Length=290, Percent_Identity=43.448275862069, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI87082152, Length=313, Percent_Identity=38.6581469648562, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1787583, Length=310, Percent_Identity=36.4516129032258, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI87081872, Length=307, Percent_Identity=35.8306188925081, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI87081858, Length=446, Percent_Identity=28.2511210762332, Blast_Score=140, Evalue=3e-34,
Organism=Escherichia coli, GI1789828, Length=260, Percent_Identity=34.6153846153846, Blast_Score=127, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR002197
- InterPro:   IPR016040
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR002078 [H]

Pfam domain/function: PF02954 HTH_8; PF00989 PAS; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 60603; Mature: 60603

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS50112 PAS ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKRLMIVGAGKGGTEVLNMLMDSPLATIIAVVDSDASAEGIALAKSYGISVADDWKQYA
CCCEEEEEECCCCHHHHHHHHHCCCHHHHEEEECCCCCCCCEEEHHHCCCCCHHHHHHHH
DATLDFVIETTGKHQTFEQLKDHFKDAVVLPGEFIRIVVERLKQNQQMLEAIIECTSEAI
CCCEEEEEECCCCHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVADHHGNTILINPSYTRLTGFKKEDVVDKPATADIGMQESVHLKVLSTGQGVRDIRMQI
HHHHCCCCEEEECCCHHHHCCCCHHCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHH
GATRRDILVNAEPIIVEGKLRGSVGVIRDVSQIRALRDELQEAKTRIRNLEAKYQFADII
CCCCCCEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
ATSDAMRFVIEQAKLAAVTPVTVLIRGESGTGKELFAHAIHGESPRKYKKFIRVNCAAIS
HHHHHHHHHHHHHHHHEECEEEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHEEHHHCC
PTLLESELFGYEDGAFSGARRGGKTGYFEEADGGSLFLDEIGELPLDVQVKLLRVLQENE
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHCCCCCHHHHHHHHHHHCCC
IVRVGGTTAIPIDVRIIAATNADLEQKVADGTFREDLYYRINRMPIHIPPLRERLNELEA
EEEECCCEEEEEEEEEEEECCCCHHHHHHCCCHHHHHHHHHCCCCEECCHHHHHHHHHHH
LCERIIRKLNQEYGRSVRGVDEDALSLLKSQHWKGNVRELENVIGRAMIYMASSEQWIAP
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHEEEEEECCCCCCCC
HHLQLSNRSTSQKVVESGTFHEAVVKYERDLIERTIEEAGGNKSEAARRLKISIRTLYNK
CEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LGTTQ
HCCCC
>Mature Secondary Structure
MNKRLMIVGAGKGGTEVLNMLMDSPLATIIAVVDSDASAEGIALAKSYGISVADDWKQYA
CCCEEEEEECCCCHHHHHHHHHCCCHHHHEEEECCCCCCCCEEEHHHCCCCCHHHHHHHH
DATLDFVIETTGKHQTFEQLKDHFKDAVVLPGEFIRIVVERLKQNQQMLEAIIECTSEAI
CCCEEEEEECCCCHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVADHHGNTILINPSYTRLTGFKKEDVVDKPATADIGMQESVHLKVLSTGQGVRDIRMQI
HHHHCCCCEEEECCCHHHHCCCCHHCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHH
GATRRDILVNAEPIIVEGKLRGSVGVIRDVSQIRALRDELQEAKTRIRNLEAKYQFADII
CCCCCCEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
ATSDAMRFVIEQAKLAAVTPVTVLIRGESGTGKELFAHAIHGESPRKYKKFIRVNCAAIS
HHHHHHHHHHHHHHHHEECEEEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHEEHHHCC
PTLLESELFGYEDGAFSGARRGGKTGYFEEADGGSLFLDEIGELPLDVQVKLLRVLQENE
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHCCCCCHHHHHHHHHHHCCC
IVRVGGTTAIPIDVRIIAATNADLEQKVADGTFREDLYYRINRMPIHIPPLRERLNELEA
EEEECCCEEEEEEEEEEEECCCCHHHHHHCCCHHHHHHHHHCCCCEECCHHHHHHHHHHH
LCERIIRKLNQEYGRSVRGVDEDALSLLKSQHWKGNVRELENVIGRAMIYMASSEQWIAP
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHEEEEEECCCCCCCC
HHLQLSNRSTSQKVVESGTFHEAVVKYERDLIERTIEEAGGNKSEAARRLKISIRTLYNK
CEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LGTTQ
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]