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The map label for this gene is bfmBC [H]

Identifier: 229916239

GI number: 229916239

Start: 461384

End: 462793

Strand: Reverse

Name: bfmBC [H]

Synonym: EAT1b_0508

Alternate gene names: 229916239

Gene position: 462793-461384 (Counterclockwise)

Preceding gene: 229916240

Following gene: 229916238

Centisome position: 15.43

GC content: 51.42

Gene sequence:

>1410_bases
ATGGCACGAGAATTTGATGTTGTCGTCATCGGTGGAGGACCAGGAGGATATGTGGCTGCAATCAAGGCGGCCCATGCAGG
AAAATCTGTCGCAATCGTCGAGGCGAGAAAGTTAGGCGGCACCTGCTTACATAGAGGATGCATCCCGACGAAGGCACTTT
TAAAAGCTGCCCATGTCTATCAAACGGCGAAGCAAAGTGCGACGTATGGCGTGGAGACGGGAGATGTGACGTTCAATATG
GAACGCGCTCAAACGTATAAACGTGATCTTGTGGCTGGTCTTGAAAAGGGAATCGAGCATTTGATGAAACAAGGGAAAAT
CGAAGTGTTTCGCGGAAAAGCCTCGATCCTTGGACCAAGCATCTTCTCACCTCAACCAGGGACTGTATCCGTTGAAGATG
AAAGCGGTGAATCAGAACTGATCTTGCCGAATCAATTGATTATCGCGACAGGGTCGATTCCTCGTGAACTTCCTGGATTG
CCGTTCGATCACAAGCGGATTTTGAATTCAGACGACCTCCTCAATTTTGAAACGCTTCCTGAATCGATTGCGATTGTCGG
TGGCGGGGTCATCGGAGTTGAATGGGCGTCCATGCTCGTCGATTTAGATGTGAACGTGACGTTGATCGAAGTAGGAGATC
GATTACTCCCACTCGAGGACAAAGCAGTATCCCGTGAAGTGGAGCGTCTATTGAAGAAACGTGGCGTCCGTGTGAAGAAG
AGCGTGACGGTCGACGCTGAACGAACATTGATTCACGAAAATGCAGTGGAGCTTGCTGTCGGGGAAGAGATGTTACAAGT
AGACTGTGTCCTCGTCTCGGTTGGCCGAGTTGCCAACACGGAAGACTTAGGACTTCAAAACACTTCTATCGTCGTAGAGA
ACGGGATCATTCAAGTCGATAGTCAGTATCGGACGAAAGAACGTCATATCTTCGCAATCGGGGATTGCATCGGAAAGTTA
CAACTCGCCCACGTCGCGTCGGCAGAAGGTGTGAAAGCGGTCGAGACGATTCTTGGTCTTGAACCAACACCGCTCGATTA
TGCGCTCATCCCACGCTGTGTATACAGTGTCCCTGAAGTCGCTTCGGTCGGCTTGACAGAAGAGGCGGCAAAAGAGGCCG
GACATGATGTGAAGACGGGGACGTATCGATTCAACGGACTCGGGAAAGCGCGGATTGAAGGACAAGCCGACGGATTCGTC
AAACTCGTCTCGGACAAAGAAACAGACGACCTTCTCGGTGTTCATATCGTCGGACCGAAAGCGACCGAACTCATCACAGA
AGGTGGACTCGCGCTCGTCTTGAACGCGACGGCTTGGGAGATGGGACAACTTGTGCACCCGCATCCTGCACTGAGCGAAG
CCTTCCAAGAAGCGGCTCTTGCCGTCGATGGATTACCGATTCACGCCTAA

Upstream 100 bases:

>100_bases
CACGTATATCCGGGTGAAGATGAGCTTAAAGCGTTGGCTGAAGGTGCACTTCGCGTCATCGAGAAGGAAGAGACCGCACG
AGTTTATGAGGGGGATACAC

Downstream 100 bases:

>100_bases
GGAGGAACAAGGATGGAACAGATGACTGTAAACTCATATGAAGAGCTTGGTTTAACAAAACAAGATTTATTGGCGATGTA
CGAAACGATGGTACGTGCCC

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of branched-chain alpha-keto acid dehydrogenase complex; LPD-Val [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MAREFDVVVIGGGPGGYVAAIKAAHAGKSVAIVEARKLGGTCLHRGCIPTKALLKAAHVYQTAKQSATYGVETGDVTFNM
ERAQTYKRDLVAGLEKGIEHLMKQGKIEVFRGKASILGPSIFSPQPGTVSVEDESGESELILPNQLIIATGSIPRELPGL
PFDHKRILNSDDLLNFETLPESIAIVGGGVIGVEWASMLVDLDVNVTLIEVGDRLLPLEDKAVSREVERLLKKRGVRVKK
SVTVDAERTLIHENAVELAVGEEMLQVDCVLVSVGRVANTEDLGLQNTSIVVENGIIQVDSQYRTKERHIFAIGDCIGKL
QLAHVASAEGVKAVETILGLEPTPLDYALIPRCVYSVPEVASVGLTEEAAKEAGHDVKTGTYRFNGLGKARIEGQADGFV
KLVSDKETDDLLGVHIVGPKATELITEGGLALVLNATAWEMGQLVHPHPALSEAFQEAALAVDGLPIHA

Sequences:

>Translated_469_residues
MAREFDVVVIGGGPGGYVAAIKAAHAGKSVAIVEARKLGGTCLHRGCIPTKALLKAAHVYQTAKQSATYGVETGDVTFNM
ERAQTYKRDLVAGLEKGIEHLMKQGKIEVFRGKASILGPSIFSPQPGTVSVEDESGESELILPNQLIIATGSIPRELPGL
PFDHKRILNSDDLLNFETLPESIAIVGGGVIGVEWASMLVDLDVNVTLIEVGDRLLPLEDKAVSREVERLLKKRGVRVKK
SVTVDAERTLIHENAVELAVGEEMLQVDCVLVSVGRVANTEDLGLQNTSIVVENGIIQVDSQYRTKERHIFAIGDCIGKL
QLAHVASAEGVKAVETILGLEPTPLDYALIPRCVYSVPEVASVGLTEEAAKEAGHDVKTGTYRFNGLGKARIEGQADGFV
KLVSDKETDDLLGVHIVGPKATELITEGGLALVLNATAWEMGQLVHPHPALSEAFQEAALAVDGLPIHA
>Mature_468_residues
AREFDVVVIGGGPGGYVAAIKAAHAGKSVAIVEARKLGGTCLHRGCIPTKALLKAAHVYQTAKQSATYGVETGDVTFNME
RAQTYKRDLVAGLEKGIEHLMKQGKIEVFRGKASILGPSIFSPQPGTVSVEDESGESELILPNQLIIATGSIPRELPGLP
FDHKRILNSDDLLNFETLPESIAIVGGGVIGVEWASMLVDLDVNVTLIEVGDRLLPLEDKAVSREVERLLKKRGVRVKKS
VTVDAERTLIHENAVELAVGEEMLQVDCVLVSVGRVANTEDLGLQNTSIVVENGIIQVDSQYRTKERHIFAIGDCIGKLQ
LAHVASAEGVKAVETILGLEPTPLDYALIPRCVYSVPEVASVGLTEEAAKEAGHDVKTGTYRFNGLGKARIEGQADGFVK
LVSDKETDDLLGVHIVGPKATELITEGGLALVLNATAWEMGQLVHPHPALSEAFQEAALAVDGLPIHA

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=469, Percent_Identity=38.1663113006397, Blast_Score=285, Evalue=6e-77,
Organism=Homo sapiens, GI50301238, Length=467, Percent_Identity=31.9057815845824, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI33519430, Length=464, Percent_Identity=24.3534482758621, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI33519428, Length=464, Percent_Identity=24.3534482758621, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI33519426, Length=464, Percent_Identity=24.3534482758621, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI148277071, Length=464, Percent_Identity=24.3534482758621, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI148277065, Length=464, Percent_Identity=24.3534482758621, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI22035672, Length=476, Percent_Identity=27.5210084033613, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI291045266, Length=481, Percent_Identity=25.1559251559252, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI291045268, Length=477, Percent_Identity=23.2704402515723, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1786307, Length=462, Percent_Identity=36.5800865800866, Blast_Score=255, Evalue=5e-69,
Organism=Escherichia coli, GI87082354, Length=464, Percent_Identity=29.0948275862069, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI87081717, Length=461, Percent_Identity=28.6334056399132, Blast_Score=154, Evalue=8e-39,
Organism=Escherichia coli, GI1789915, Length=454, Percent_Identity=27.5330396475771, Blast_Score=154, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI32565766, Length=467, Percent_Identity=39.4004282655246, Blast_Score=290, Evalue=1e-78,
Organism=Caenorhabditis elegans, GI17557007, Length=476, Percent_Identity=26.890756302521, Blast_Score=134, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI71983429, Length=470, Percent_Identity=27.8723404255319, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI71983419, Length=470, Percent_Identity=27.8723404255319, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI71982272, Length=493, Percent_Identity=25.7606490872211, Blast_Score=101, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6321091, Length=479, Percent_Identity=37.9958246346555, Blast_Score=277, Evalue=3e-75,
Organism=Saccharomyces cerevisiae, GI6325240, Length=483, Percent_Identity=26.9151138716356, Blast_Score=173, Evalue=5e-44,
Organism=Saccharomyces cerevisiae, GI6325166, Length=478, Percent_Identity=26.7782426778243, Blast_Score=153, Evalue=6e-38,
Organism=Drosophila melanogaster, GI21358499, Length=479, Percent_Identity=39.0396659707724, Blast_Score=287, Evalue=1e-77,
Organism=Drosophila melanogaster, GI24640553, Length=494, Percent_Identity=29.1497975708502, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24640549, Length=494, Percent_Identity=29.1497975708502, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24640551, Length=494, Percent_Identity=29.1497975708502, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI17737741, Length=480, Percent_Identity=26.25, Blast_Score=125, Evalue=8e-29,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50048; Mature: 49917

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAREFDVVVIGGGPGGYVAAIKAAHAGKSVAIVEARKLGGTCLHRGCIPTKALLKAAHVY
CCCEEEEEEEECCCCCEEEEEEHHCCCCEEEEEEEHHCCCHHHHCCCCCHHHHHHHHHHH
QTAKQSATYGVETGDVTFNMERAQTYKRDLVAGLEKGIEHLMKQGKIEVFRGKASILGPS
HHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCC
IFSPQPGTVSVEDESGESELILPNQLIIATGSIPRELPGLPFDHKRILNSDDLLNFETLP
CCCCCCCEEEEECCCCCCEEECCCEEEEEECCCCHHCCCCCCCHHHHCCCCCCCCHHHCC
ESIAIVGGGVIGVEWASMLVDLDVNVTLIEVGDRLLPLEDKAVSREVERLLKKRGVRVKK
CEEEEECCCEEHHHHHHEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEE
SVTVDAERTLIHENAVELAVGEEMLQVDCVLVSVGRVANTEDLGLQNTSIVVENGIIQVD
EEEECHHHHHHHCCCHHHHHCHHHHHHEEEEEECCCCCCCCCCCCCCCEEEEECCEEEEC
SQYRTKERHIFAIGDCIGKLQLAHVASAEGVKAVETILGLEPTPLDYALIPRCVYSVPEV
CCCCCCCCEEEEEEHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCCH
ASVGLTEEAAKEAGHDVKTGTYRFNGLGKARIEGQADGFVKLVSDKETDDLLGVHIVGPK
HHCCCCHHHHHHCCCCCCCCEEEECCCCCEEECCCCCCEEEEECCCCCCCEEEEEEECCC
ATELITEGGLALVLNATAWEMGQLVHPHPALSEAFQEAALAVDGLPIHA
HHHHHHCCCEEEEEECCHHHHCCCCCCCHHHHHHHHHHHHEECCCCCCC
>Mature Secondary Structure 
AREFDVVVIGGGPGGYVAAIKAAHAGKSVAIVEARKLGGTCLHRGCIPTKALLKAAHVY
CCEEEEEEEECCCCCEEEEEEHHCCCCEEEEEEEHHCCCHHHHCCCCCHHHHHHHHHHH
QTAKQSATYGVETGDVTFNMERAQTYKRDLVAGLEKGIEHLMKQGKIEVFRGKASILGPS
HHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCC
IFSPQPGTVSVEDESGESELILPNQLIIATGSIPRELPGLPFDHKRILNSDDLLNFETLP
CCCCCCCEEEEECCCCCCEEECCCEEEEEECCCCHHCCCCCCCHHHHCCCCCCCCHHHCC
ESIAIVGGGVIGVEWASMLVDLDVNVTLIEVGDRLLPLEDKAVSREVERLLKKRGVRVKK
CEEEEECCCEEHHHHHHEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEE
SVTVDAERTLIHENAVELAVGEEMLQVDCVLVSVGRVANTEDLGLQNTSIVVENGIIQVD
EEEECHHHHHHHCCCHHHHHCHHHHHHEEEEEECCCCCCCCCCCCCCCEEEEECCEEEEC
SQYRTKERHIFAIGDCIGKLQLAHVASAEGVKAVETILGLEPTPLDYALIPRCVYSVPEV
CCCCCCCCEEEEEEHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCCH
ASVGLTEEAAKEAGHDVKTGTYRFNGLGKARIEGQADGFVKLVSDKETDDLLGVHIVGPK
HHCCCCHHHHHHCCCCCCCCEEEECCCCCEEECCCCCCEEEEECCCCCCCEEEEEEECCC
ATELITEGGLALVLNATAWEMGQLVHPHPALSEAFQEAALAVDGLPIHA
HHHHHHCCCEEEEEECCHHHHCCCCCCCHHHHHHHHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 8504804 [H]