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The map label for this gene is rnz

Identifier: 229916219

GI number: 229916219

Start: 445053

End: 445958

Strand: Reverse

Name: rnz

Synonym: EAT1b_0488

Alternate gene names: 229916219

Gene position: 445958-445053 (Counterclockwise)

Preceding gene: 229916221

Following gene: 229916218

Centisome position: 14.87

GC content: 50.22

Gene sequence:

>906_bases
ATGAAGTTACAGTTTTTAGGAACTGGATCGGGGATGCCTTCGAAACAGCGCAACGTATCGGGGCTTGCTCTCGATTTATC
GAACGTGACCTGGCTGATTGATTGTGGCGAGGGCACGCAACACCAAATGCTCTATACGAAAATCAAACCGAGAAAAGTGA
CAGCGGTATGGGTTACGCATCTTCATGGTGACCATGTCTTCGGATTGCCCGGATTCTTATCGACTCGTTCGGCACTCGAT
GGAACCGCCCCCTTGACCGTCTATGGGCCAAAAGGATTGAAAAAATGGCTTGAAGCTACTTTGCGTGTCACAGGCTCACA
TCTAGGATACGAGCTGACCGTGACCGAGTACTCGGACGGGGACACATTCATACAAGACGACCATCTCGTCACTGTGCGAA
AATTAGAGCATCGGTTCCCTTCATTCGGTTTCCGAATCGATGGTCCAGAAAAACCGGGCATGTTACGCGTCGATCTTGTA
AGAGAACTCGGTGTTCCAAGTGGACCGATTTATCGAACCATCAAAGAATCAGACCGATTTGAATTTGAAGGAAAATGGTA
TGACTCTAAAGAATACGTCACTGAACCGATTCCGGGAAAAATCGTAGCGATTTTAGGAGATACAATCCCTTGTGAGAACG
CGCGGAAGTTGGCGGATGGAGCGGACCTCCTCGTTCACGAGGCGACGTTTATGGAGGCGGAACAAGCGCTCGCTCGGAAG
TATGGTCACTCGACGACACGTGAAGCGACCGCGCTAGCGAATGCGTGCGCCGTCGGCAAGCTGATTGTGACACACATCTC
AGCGCGCTATGTCGGGCGAGAGGAAGAATTTGTTCAAGAAGTCGTGTCGGATTTTCCATCGAGTCTCGTTGCGACGGACT
TTTTAGAATATACAGTGGGGGATTGA

Upstream 100 bases:

>100_bases
CGATTCGGGTTGTTCTGCCTTGGTCATCACAAATCACATCCTTTTCAGCGATTACGTCAAGTATAGCATGGAACGGCTTG
AAGAAAGGACTTAAATATTT

Downstream 100 bases:

>100_bases
TGTGACCATTCAACTCTATGTATGGGCAGCCTTTTTAGTGATTAGCGTTCTATTAGGGATTCGATTAAATCAATTGAAAG
AGCCGGAAGCGAACTTCTTT

Product: ribonuclease Z

Products: NA

Alternate protein names: RNase Z; tRNA 3 endonuclease; tRNase Z

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MKLQFLGTGSGMPSKQRNVSGLALDLSNVTWLIDCGEGTQHQMLYTKIKPRKVTAVWVTHLHGDHVFGLPGFLSTRSALD
GTAPLTVYGPKGLKKWLEATLRVTGSHLGYELTVTEYSDGDTFIQDDHLVTVRKLEHRFPSFGFRIDGPEKPGMLRVDLV
RELGVPSGPIYRTIKESDRFEFEGKWYDSKEYVTEPIPGKIVAILGDTIPCENARKLADGADLLVHEATFMEAEQALARK
YGHSTTREATALANACAVGKLIVTHISARYVGREEEFVQEVVSDFPSSLVATDFLEYTVGD

Sequences:

>Translated_301_residues
MKLQFLGTGSGMPSKQRNVSGLALDLSNVTWLIDCGEGTQHQMLYTKIKPRKVTAVWVTHLHGDHVFGLPGFLSTRSALD
GTAPLTVYGPKGLKKWLEATLRVTGSHLGYELTVTEYSDGDTFIQDDHLVTVRKLEHRFPSFGFRIDGPEKPGMLRVDLV
RELGVPSGPIYRTIKESDRFEFEGKWYDSKEYVTEPIPGKIVAILGDTIPCENARKLADGADLLVHEATFMEAEQALARK
YGHSTTREATALANACAVGKLIVTHISARYVGREEEFVQEVVSDFPSSLVATDFLEYTVGD
>Mature_301_residues
MKLQFLGTGSGMPSKQRNVSGLALDLSNVTWLIDCGEGTQHQMLYTKIKPRKVTAVWVTHLHGDHVFGLPGFLSTRSALD
GTAPLTVYGPKGLKKWLEATLRVTGSHLGYELTVTEYSDGDTFIQDDHLVTVRKLEHRFPSFGFRIDGPEKPGMLRVDLV
RELGVPSGPIYRTIKESDRFEFEGKWYDSKEYVTEPIPGKIVAILGDTIPCENARKLADGADLLVHEATFMEAEQALARK
YGHSTTREATALANACAVGKLIVTHISARYVGREEEFVQEVVSDFPSSLVATDFLEYTVGD

Specific function: Zinc phosphodiesterase, which displays some tRNA 3'- processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA

COG id: COG1234

COG function: function code R; Metal-dependent hydrolases of the beta-lactamase superfamily III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase Z family

Homologues:

Organism=Homo sapiens, GI8922122, Length=357, Percent_Identity=32.2128851540616, Blast_Score=182, Evalue=4e-46,
Organism=Homo sapiens, GI260166619, Length=105, Percent_Identity=43.8095238095238, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI260166622, Length=105, Percent_Identity=43.8095238095238, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI145553959, Length=105, Percent_Identity=43.8095238095238, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI87082078, Length=307, Percent_Identity=43.3224755700326, Blast_Score=263, Evalue=7e-72,
Organism=Drosophila melanogaster, GI24652403, Length=308, Percent_Identity=27.5974025974026, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNZ_EXISA (C4L3C2)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002884865.1
- ProteinModelPortal:   C4L3C2
- GeneID:   7871306
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_0488
- OMA:   HSTSTMA
- ProtClustDB:   CLSK2489211
- HAMAP:   MF_01818
- InterPro:   IPR001279
- InterPro:   IPR013471
- SMART:   SM00849
- TIGRFAMs:   TIGR02651

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.26.11

Molecular weight: Translated: 33319; Mature: 33319

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLQFLGTGSGMPSKQRNVSGLALDLSNVTWLIDCGEGTQHQMLYTKIKPRKVTAVWVTH
CEEEEEECCCCCCCCCCCCCEEEEEECCEEEEEECCCCCCCCEEEEECCCCEEEEEEEEE
LHGDHVFGLPGFLSTRSALDGTAPLTVYGPKGLKKWLEATLRVTGSHLGYELTVTEYSDG
ECCCEEECCCCHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCEEEEEEEECCC
DTFIQDDHLVTVRKLEHRFPSFGFRIDGPEKPGMLRVDLVRELGVPSGPIYRTIKESDRF
CCEEECCCEEHHHHHHHCCCCCCEEECCCCCCCEEEHHHHHHCCCCCCCHHHHHHCCCCE
EFEGKWYDSKEYVTEPIPGKIVAILGDTIPCENARKLADGADLLVHEATFMEAEQALARK
EECCEEECCHHHCCCCCCCEEEEEECCCCCCCCHHHHCCCCCEEEEHHHHHHHHHHHHHH
YGHSTTREATALANACAVGKLIVTHISARYVGREEEFVQEVVSDFPSSLVATDFLEYTVG
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHCC
D
C
>Mature Secondary Structure
MKLQFLGTGSGMPSKQRNVSGLALDLSNVTWLIDCGEGTQHQMLYTKIKPRKVTAVWVTH
CEEEEEECCCCCCCCCCCCCEEEEEECCEEEEEECCCCCCCCEEEEECCCCEEEEEEEEE
LHGDHVFGLPGFLSTRSALDGTAPLTVYGPKGLKKWLEATLRVTGSHLGYELTVTEYSDG
ECCCEEECCCCHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCEEEEEEEECCC
DTFIQDDHLVTVRKLEHRFPSFGFRIDGPEKPGMLRVDLVRELGVPSGPIYRTIKESDRF
CCEEECCCEEHHHHHHHCCCCCCEEECCCCCCCEEEHHHHHHCCCCCCCHHHHHHCCCCE
EFEGKWYDSKEYVTEPIPGKIVAILGDTIPCENARKLADGADLLVHEATFMEAEQALARK
EECCEEECCHHHCCCCCCCEEEEEECCCCCCCCHHHHCCCCCEEEEHHHHHHHHHHHHHH
YGHSTTREATALANACAVGKLIVTHISARYVGREEEFVQEVVSDFPSSLVATDFLEYTVG
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHCC
D
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA