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The map label for this gene is yqjE [H]

Identifier: 229916221

GI number: 229916221

Start: 446252

End: 447379

Strand: Reverse

Name: yqjE [H]

Synonym: EAT1b_0490

Alternate gene names: 229916221

Gene position: 447379-446252 (Counterclockwise)

Preceding gene: 229916222

Following gene: 229916219

Centisome position: 14.91

GC content: 51.24

Gene sequence:

>1128_bases
ATGGTAAACGAGACACGCGTGCTCGACACATTTTTAGGGTTGGTCGGCATCGATTCAGAAACGAAGGAAGAAGCGGTCAT
CTGTGAACATTTGAAAGGTGTATTCACAGAGCTTGGCTGCGACGTCTACGAGGACGACGCATCAACGAAGACGGCACACT
CTGGTAACAACTTGATCATCACGTTGCCTGCAACGAAAGAAAATGTTGATCCGATTTACTTTACGGCTCACATGGATACG
GTCACTCCAGGTAAAGGAATCAAGACGAGCATCAAAGACGGATATGTCGTCACGGATGGCACGACGATTCTCGGGGCAGA
CGACAAGACGGGCCTCACGGCGATGATCGAACTCGTGCGAGTATTGAAAGAAAAGCAAATTCCACACGGTCAAATCCAGT
TCGTGATCACGGTCGGGGAAGAATCAGGTCTCGTCGGTGCGAAAGTATTGGATTTATCGAAAATCGATGCGAAGTTCGGT
TTTGCCCTTGATTCGGATGGACCTGTTGGAGACATCATTGTTGCCGCTCCGACACAAGCAAAAGTGAACGCGAAGATTTA
CGGGAAAACGGCTCATGCTGGTGTCGCACCGGAAAAAGGAATCTCGGCGATTCAAATCGCAGCGAAAGCGATCGCGAAGA
TGCCACTCGGTCGTATCGATGAAGAAACGACAGCCAACATCGGACGCTTCGAAGGGGGCCGCGCGACAAACATCGTGTGT
GACTATGTCGAAGTTCTTGCTGAGGCCCGTTCGCTCATCCATGAGAAGATGGAGAAGCAGGCAGAAACGATGAAACAAGC
GTACATCGAGGTCGCTGAGTCGTTCGGTGGTCGTGCCGAAGTGGACATCGACATCATGTATCCTGGATTCAAGTTTGAGG
AAGGGGACGAAGTCGTTGAGGTGGCGAAACGTGCGGCTCAGACAATCGGACGTCCGACTCGAATCTTACACTCAGGGGGC
GGTTCGGATGCGAACGTCATCGCCGGTGGGGGAATCCCGACAGTCAACTTGGCGGTCGGCTATGAAGAGATTCATACGAC
GAACGAACGGATGCCACTTGAAGAGTTATACAAACTCGTCGAGTATGTCGTGACGATCGTCGAAGAAGTCGCATCACCTA
AGCAATAA

Upstream 100 bases:

>100_bases
ATACGTGTTTGCCATCATTGTACAGAATGGAAAAGATTCGTGCAATCCAGGGGAGTTTGATATAATGGTCAAGAAATGTT
TGGATGGAGGGATGTTGTTT

Downstream 100 bases:

>100_bases
AAAAAGAGCCGACCATTCGTCGGCTCTTCGTATGTGTTCGTACTCGTTATGCTGCGAACTCCAGTGATTCTAAAATCAAC
TCGTCTGTGCAATGAAGTTC

Product: peptidase T-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MVNETRVLDTFLGLVGIDSETKEEAVICEHLKGVFTELGCDVYEDDASTKTAHSGNNLIITLPATKENVDPIYFTAHMDT
VTPGKGIKTSIKDGYVVTDGTTILGADDKTGLTAMIELVRVLKEKQIPHGQIQFVITVGEESGLVGAKVLDLSKIDAKFG
FALDSDGPVGDIIVAAPTQAKVNAKIYGKTAHAGVAPEKGISAIQIAAKAIAKMPLGRIDEETTANIGRFEGGRATNIVC
DYVEVLAEARSLIHEKMEKQAETMKQAYIEVAESFGGRAEVDIDIMYPGFKFEEGDEVVEVAKRAAQTIGRPTRILHSGG
GSDANVIAGGGIPTVNLAVGYEEIHTTNERMPLEELYKLVEYVVTIVEEVASPKQ

Sequences:

>Translated_375_residues
MVNETRVLDTFLGLVGIDSETKEEAVICEHLKGVFTELGCDVYEDDASTKTAHSGNNLIITLPATKENVDPIYFTAHMDT
VTPGKGIKTSIKDGYVVTDGTTILGADDKTGLTAMIELVRVLKEKQIPHGQIQFVITVGEESGLVGAKVLDLSKIDAKFG
FALDSDGPVGDIIVAAPTQAKVNAKIYGKTAHAGVAPEKGISAIQIAAKAIAKMPLGRIDEETTANIGRFEGGRATNIVC
DYVEVLAEARSLIHEKMEKQAETMKQAYIEVAESFGGRAEVDIDIMYPGFKFEEGDEVVEVAKRAAQTIGRPTRILHSGG
GSDANVIAGGGIPTVNLAVGYEEIHTTNERMPLEELYKLVEYVVTIVEEVASPKQ
>Mature_375_residues
MVNETRVLDTFLGLVGIDSETKEEAVICEHLKGVFTELGCDVYEDDASTKTAHSGNNLIITLPATKENVDPIYFTAHMDT
VTPGKGIKTSIKDGYVVTDGTTILGADDKTGLTAMIELVRVLKEKQIPHGQIQFVITVGEESGLVGAKVLDLSKIDAKFG
FALDSDGPVGDIIVAAPTQAKVNAKIYGKTAHAGVAPEKGISAIQIAAKAIAKMPLGRIDEETTANIGRFEGGRATNIVC
DYVEVLAEARSLIHEKMEKQAETMKQAYIEVAESFGGRAEVDIDIMYPGFKFEEGDEVVEVAKRAAQTIGRPTRILHSGG
GSDANVIAGGGIPTVNLAVGYEEIHTTNERMPLEELYKLVEYVVTIVEEVASPKQ

Specific function: Could be a peptidase [H]

COG id: COG2195

COG function: function code E; Di- and tripeptidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family [H]

Homologues:

Organism=Escherichia coli, GI1787372, Length=413, Percent_Identity=27.1186440677966, Blast_Score=110, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR010162
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: 3.4.11.4 [C]

Molecular weight: Translated: 40177; Mature: 40177

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS00758 ARGE_DAPE_CPG2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNETRVLDTFLGLVGIDSETKEEAVICEHLKGVFTELGCDVYEDDASTKTAHSGNNLII
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCEEEE
TLPATKENVDPIYFTAHMDTVTPGKGIKTSIKDGYVVTDGTTILGADDKTGLTAMIELVR
EECCCCCCCCEEEEEEEECCCCCCCCCEECCCCCEEEECCCEEEECCCCCCHHHHHHHHH
VLKEKQIPHGQIQFVITVGEESGLVGAKVLDLSKIDAKFGFALDSDGPVGDIIVAAPTQA
HHHHHCCCCCCEEEEEEECCCCCCCCCEEECHHHHHHHHCEEECCCCCCCCEEEECCCCC
KVNAKIYGKTAHAGVAPEKGISAIQIAAKAIAKMPLGRIDEETTANIGRFEGGRATNIVC
EEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCHHHHH
DYVEVLAEARSLIHEKMEKQAETMKQAYIEVAESFGGRAEVDIDIMYPGFKFEEGDEVVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCHHHHH
VAKRAAQTIGRPTRILHSGGGSDANVIAGGGIPTVNLAVGYEEIHTTNERMPLEELYKLV
HHHHHHHHCCCCCEEEECCCCCCCCEEECCCCCEEEEEECHHHHHCCCCCCCHHHHHHHH
EYVVTIVEEVASPKQ
HHHHHHHHHHCCCCC
>Mature Secondary Structure
MVNETRVLDTFLGLVGIDSETKEEAVICEHLKGVFTELGCDVYEDDASTKTAHSGNNLII
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCEEEE
TLPATKENVDPIYFTAHMDTVTPGKGIKTSIKDGYVVTDGTTILGADDKTGLTAMIELVR
EECCCCCCCCEEEEEEEECCCCCCCCCEECCCCCEEEECCCEEEECCCCCCHHHHHHHHH
VLKEKQIPHGQIQFVITVGEESGLVGAKVLDLSKIDAKFGFALDSDGPVGDIIVAAPTQA
HHHHHCCCCCCEEEEEEECCCCCCCCCEEECHHHHHHHHCEEECCCCCCCCEEEECCCCC
KVNAKIYGKTAHAGVAPEKGISAIQIAAKAIAKMPLGRIDEETTANIGRFEGGRATNIVC
EEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCHHHHH
DYVEVLAEARSLIHEKMEKQAETMKQAYIEVAESFGGRAEVDIDIMYPGFKFEEGDEVVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCHHHHH
VAKRAAQTIGRPTRILHSGGGSDANVIAGGGIPTVNLAVGYEEIHTTNERMPLEELYKLV
HHHHHHHHCCCCCEEEECCCCCCCCEEECCCCCEEEEEECHHHHHCCCCCCCHHHHHHHH
EYVVTIVEEVASPKQ
HHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Aminopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]