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The map label for this gene is ymfI [H]

Identifier: 229916165

GI number: 229916165

Start: 396987

End: 397682

Strand: Reverse

Name: ymfI [H]

Synonym: EAT1b_0434

Alternate gene names: 229916165

Gene position: 397682-396987 (Counterclockwise)

Preceding gene: 229916166

Following gene: 229916164

Centisome position: 13.26

GC content: 53.02

Gene sequence:

>696_bases
ATGAAGACATTGATTGTTGGAGCGAGCGGTGCCATCGGACGTGCCGTCTCGCTCGCTTTTCGTGAAGATGAACTAATTTT
GCATGCGAACGATGGTCGTGAAAGACTTCGTCATTTGATTGATGATGAAAATCTATCGGCAACACTGCTCCCTTGTGACC
TAACGGACCGTGAAGCTGTTGAGTCATGGGTACAGGATATGCCGGATGTCGATCGAATGGTATACGTGGCAGGACATGCG
CTCCATGGTCTCGTCGTCGACCAGTCAATGGACGAGCTCGACCGGCTATATGATGTACATGTTCGAGCGATGATCCGACT
CATCCAGTCCGTCGTTGCGAGCAAGCCTTACAATCATCATCTGGATATCGTGATCGTCTCAAGCGTATGGGGAGAGGTCG
GTGCGGCGGCTGAGGTGTTTTATTCCACCGTCAAAGCGGCACAGCTCGGCTTCGTCAAAGCGTTCGCTCGAGAGGCTGGC
CCGTTCAATGTTCGAATCAATGCGGTGACACCGGGGTGGATTGATACGCCAATGAATGATACGGTAGAAGAGTCGAAACA
ATCCGTGCTCGACGACATTCCACTAGGACGTCTCGGTGCGGCAAAGGACGTGGCCAACACCATCCGTTTCTTGTGTTCAC
CGGACGCCTCGTATATGACGGGCACGGTGTTACGCATCGACGGCGGTTGGCAATGA

Upstream 100 bases:

>100_bases
TCCCGACTTTGATTGATGCCATCACGCTCGATGAACTATACGAAGCATACGAGCGCTTGTTCCGCCGTGAACAACGAACG
GTGTGCGTCGTCAAAAAACC

Downstream 100 bases:

>100_bases
TCCGATCCATGTAGATAAACCTGTTCTTTTCAATTCTTCTAAAATAATTTAAAATAGGCAAGGTAGCGAACTCGCTCGAA
AGGATGGTTGGGGATGGAAC

Product: short-chain dehydrogenase/reductase SDR

Products: 3-oxoacyl-[acyl-carrier protein]; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MKTLIVGASGAIGRAVSLAFREDELILHANDGRERLRHLIDDENLSATLLPCDLTDREAVESWVQDMPDVDRMVYVAGHA
LHGLVVDQSMDELDRLYDVHVRAMIRLIQSVVASKPYNHHLDIVIVSSVWGEVGAAAEVFYSTVKAAQLGFVKAFAREAG
PFNVRINAVTPGWIDTPMNDTVEESKQSVLDDIPLGRLGAAKDVANTIRFLCSPDASYMTGTVLRIDGGWQ

Sequences:

>Translated_231_residues
MKTLIVGASGAIGRAVSLAFREDELILHANDGRERLRHLIDDENLSATLLPCDLTDREAVESWVQDMPDVDRMVYVAGHA
LHGLVVDQSMDELDRLYDVHVRAMIRLIQSVVASKPYNHHLDIVIVSSVWGEVGAAAEVFYSTVKAAQLGFVKAFAREAG
PFNVRINAVTPGWIDTPMNDTVEESKQSVLDDIPLGRLGAAKDVANTIRFLCSPDASYMTGTVLRIDGGWQ
>Mature_231_residues
MKTLIVGASGAIGRAVSLAFREDELILHANDGRERLRHLIDDENLSATLLPCDLTDREAVESWVQDMPDVDRMVYVAGHA
LHGLVVDQSMDELDRLYDVHVRAMIRLIQSVVASKPYNHHLDIVIVSSVWGEVGAAAEVFYSTVKAAQLGFVKAFAREAG
PFNVRINAVTPGWIDTPMNDTVEESKQSVLDDIPLGRLGAAKDVANTIRFLCSPDASYMTGTVLRIDGGWQ

Specific function: Fatty acid biosynthesis pathway; first reduction step. [C]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI15277342, Length=252, Percent_Identity=30.1587301587302, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI66933014, Length=222, Percent_Identity=28.3783783783784, Blast_Score=92, Evalue=3e-19,
Organism=Homo sapiens, GI32483357, Length=198, Percent_Identity=30.8080808080808, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI40254992, Length=233, Percent_Identity=27.0386266094421, Blast_Score=81, Evalue=6e-16,
Organism=Homo sapiens, GI7705925, Length=241, Percent_Identity=29.4605809128631, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI5031737, Length=239, Percent_Identity=26.7782426778243, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI126723750, Length=188, Percent_Identity=28.7234042553192, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI4503301, Length=108, Percent_Identity=32.4074074074074, Blast_Score=65, Evalue=6e-11,
Organism=Homo sapiens, GI59889578, Length=207, Percent_Identity=27.0531400966184, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1787335, Length=234, Percent_Identity=30.7692307692308, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI2367365, Length=233, Percent_Identity=29.6137339055794, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87082100, Length=243, Percent_Identity=28.8065843621399, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI87082160, Length=240, Percent_Identity=25.8333333333333, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1787905, Length=236, Percent_Identity=27.9661016949153, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1789378, Length=243, Percent_Identity=25.1028806584362, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788459, Length=175, Percent_Identity=30.8571428571429, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI2367175, Length=242, Percent_Identity=27.2727272727273, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1789208, Length=173, Percent_Identity=24.8554913294798, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI25147288, Length=237, Percent_Identity=27.0042194092827, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71994600, Length=251, Percent_Identity=31.0756972111554, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71994604, Length=246, Percent_Identity=30.8943089430894, Blast_Score=91, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17555706, Length=166, Percent_Identity=34.3373493975904, Blast_Score=91, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI115534694, Length=240, Percent_Identity=29.5833333333333, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17560676, Length=144, Percent_Identity=31.9444444444444, Blast_Score=70, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17561402, Length=147, Percent_Identity=32.6530612244898, Blast_Score=70, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17508651, Length=245, Percent_Identity=24.8979591836735, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17562906, Length=258, Percent_Identity=29.0697674418605, Blast_Score=68, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17562908, Length=259, Percent_Identity=29.7297297297297, Blast_Score=67, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6323882, Length=229, Percent_Identity=27.9475982532751, Blast_Score=70, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324126, Length=225, Percent_Identity=25.7777777777778, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24639444, Length=244, Percent_Identity=28.6885245901639, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21355319, Length=140, Percent_Identity=32.1428571428571, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI23397609, Length=242, Percent_Identity=30.1652892561983, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28571526, Length=243, Percent_Identity=31.2757201646091, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24643142, Length=232, Percent_Identity=28.0172413793103, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21357041, Length=167, Percent_Identity=33.5329341317365, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24644337, Length=117, Percent_Identity=40.1709401709402, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24644339, Length=243, Percent_Identity=28.8065843621399, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100

Molecular weight: Translated: 25356; Mature: 25356

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLIVGASGAIGRAVSLAFREDELILHANDGRERLRHLIDDENLSATLLPCDLTDREAV
CCEEEEECCCCHHHHEEEEEECCEEEEEECCCHHHHHHHHCCCCCCEEEEECCCCCHHHH
ESWVQDMPDVDRMVYVAGHALHGLVVDQSMDELDRLYDVHVRAMIRLIQSVVASKPYNHH
HHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
LDIVIVSSVWGEVGAAAEVFYSTVKAAQLGFVKAFAREAGPFNVRINAVTPGWIDTPMND
EEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCH
TVEESKQSVLDDIPLGRLGAAKDVANTIRFLCSPDASYMTGTVLRIDGGWQ
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCHHEEEEEEECCCCC
>Mature Secondary Structure
MKTLIVGASGAIGRAVSLAFREDELILHANDGRERLRHLIDDENLSATLLPCDLTDREAV
CCEEEEECCCCHHHHEEEEEECCEEEEEECCCHHHHHHHHCCCCCCEEEEECCCCCHHHH
ESWVQDMPDVDRMVYVAGHALHGLVVDQSMDELDRLYDVHVRAMIRLIQSVVASKPYNHH
HHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
LDIVIVSSVWGEVGAAAEVFYSTVKAAQLGFVKAFAREAGPFNVRINAVTPGWIDTPMND
EEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCH
TVEESKQSVLDDIPLGRLGAAKDVANTIRFLCSPDASYMTGTVLRIDGGWQ
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCHHEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3R)-3-hydroxyacyl-[acyl-carrier protein]; NADP+

Specific reaction: (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ = 3-oxoacyl-[acyl-carrier protein] + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]