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The map label for this gene is glnA [H]

Identifier: 229916142

GI number: 229916142

Start: 373922

End: 375262

Strand: Reverse

Name: glnA [H]

Synonym: EAT1b_0411

Alternate gene names: 229916142

Gene position: 375262-373922 (Counterclockwise)

Preceding gene: 229916143

Following gene: 229916138

Centisome position: 12.51

GC content: 49.66

Gene sequence:

>1341_bases
ATGGCCAGAAAAACGTTCACAAAAGAAGACATTAAACGCATCGCACAAGAGGAAGACGTACGCTTTATTCGTCTTCAATT
CACGGACATCCTCGGAACAATCAAAAACGTCGAGGTGCCGATCAGCCAATTGGACAAAGCACTTGATAACAAGATGATGT
TTGACGGATCGTCAATCGAAGGATTCGTCCGCATCGAGGAATCAGACATGTACTTGTATCCAGACCTCAACACATGGGTT
GTCTTCCCGTGGACAGATGGCAGCGGAAAAGTCGCTCGCTTAATTTGCGATATTTATAACCCAGACGGCACGCCGTTCAG
CGGTGACCCGCGCGGAATTCTCAAGCGTAACCTTGAGAAGATGCAAAAACTTGGTTTCACAGCGTTCAACGTTGGACCAG
AGCCAGAGTTCTTCTTGTTCAAGAAAGACGCATCAGGTCGTCCGACTCTTGAACTTAACGACCAAGGTGGATACTTCGAC
TTGGCACCAGTTGACCTTGGTGAGAACTGCCGTAAAGAGATCGTCATCGAACTTGAAAACATGGGCTTTGAAATCGAAGC
GTCTCACCACGAAGTAGCGCCAGGACAACACGAAATCGACTTCAAATACGCAGATGCTGTCACGACGGCAGATAACATTC
AAACGTTCAAACTCGTCGTCAAGACAATTGCTGCGAAATACGGTCTTCACGCTACATTCATGCCGAAGCCACTCTTCGGT
GTAAACGGTTCAGGAATGCACGCGAACATGTCACTCTTTAAAGGTGACACAAACGTCTTCTACGATCCAAACGGTGACAT
GGAACTCTCAGACACAGCTCGCGCGTTCACGGCTGGGATCTTGGAGCACGCTCGTGCCTTCACAGCGGTATGTAACCCGA
CTGTGAACTCATACAAGCGTCTCGTACCAGGCTACGAAGCACCTTGCTATGTTGCATGGAGCGCACGCAACCGTTCACCA
CTCGTTCGTGTACCAGCGGCACGTGGTCTCTCGACACGTATCGAAGTACGTTCGGTTGACCCGGCAGCGAACCCATACCT
TGCACTTGCTGCCCTCTTGGCTTCAGGTCTTGACGGAATCGAAAAAGATTTGAATGCTCCAAAACCAATCGACAGCAACA
TCTACGTGATGGACAAGCCAGAGCGTGTAGCAAACGGAATCGATGATCTCCCAGCGACACTCTTTGACGCACTCGAAGTC
CTTCGTGCGGACAAAGTCGTGATGGACTCACTCGGTGAGCATATCGGCGAACACTTCCTCGAGTTGAAAGAAATCGAGTG
GGATATGTTCCGCATGCAAGTAACAGAGTGGGAACGCGATCAGTACATGACGCTCTACTAA

Upstream 100 bases:

>100_bases
GTTTCAATAAGACGTCGCTCATTCAAGGTGAGCTTTCACGTTTCTATCGATAACGCCGCACACATCAACCATTACGCAAT
GTGAGAGGGGACATTTTACC

Downstream 100 bases:

>100_bases
TAGAAGAGATATATTAAAGAGTATGGAGCATCCCTCAATTTGGGATGCTCCATACTCTTTTTGATAAATCATAATAGCGG
AGATATCTTTATGATTCCGG

Product: glutamine synthetase, type I

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MARKTFTKEDIKRIAQEEDVRFIRLQFTDILGTIKNVEVPISQLDKALDNKMMFDGSSIEGFVRIEESDMYLYPDLNTWV
VFPWTDGSGKVARLICDIYNPDGTPFSGDPRGILKRNLEKMQKLGFTAFNVGPEPEFFLFKKDASGRPTLELNDQGGYFD
LAPVDLGENCRKEIVIELENMGFEIEASHHEVAPGQHEIDFKYADAVTTADNIQTFKLVVKTIAAKYGLHATFMPKPLFG
VNGSGMHANMSLFKGDTNVFYDPNGDMELSDTARAFTAGILEHARAFTAVCNPTVNSYKRLVPGYEAPCYVAWSARNRSP
LVRVPAARGLSTRIEVRSVDPAANPYLALAALLASGLDGIEKDLNAPKPIDSNIYVMDKPERVANGIDDLPATLFDALEV
LRADKVVMDSLGEHIGEHFLELKEIEWDMFRMQVTEWERDQYMTLY

Sequences:

>Translated_446_residues
MARKTFTKEDIKRIAQEEDVRFIRLQFTDILGTIKNVEVPISQLDKALDNKMMFDGSSIEGFVRIEESDMYLYPDLNTWV
VFPWTDGSGKVARLICDIYNPDGTPFSGDPRGILKRNLEKMQKLGFTAFNVGPEPEFFLFKKDASGRPTLELNDQGGYFD
LAPVDLGENCRKEIVIELENMGFEIEASHHEVAPGQHEIDFKYADAVTTADNIQTFKLVVKTIAAKYGLHATFMPKPLFG
VNGSGMHANMSLFKGDTNVFYDPNGDMELSDTARAFTAGILEHARAFTAVCNPTVNSYKRLVPGYEAPCYVAWSARNRSP
LVRVPAARGLSTRIEVRSVDPAANPYLALAALLASGLDGIEKDLNAPKPIDSNIYVMDKPERVANGIDDLPATLFDALEV
LRADKVVMDSLGEHIGEHFLELKEIEWDMFRMQVTEWERDQYMTLY
>Mature_445_residues
ARKTFTKEDIKRIAQEEDVRFIRLQFTDILGTIKNVEVPISQLDKALDNKMMFDGSSIEGFVRIEESDMYLYPDLNTWVV
FPWTDGSGKVARLICDIYNPDGTPFSGDPRGILKRNLEKMQKLGFTAFNVGPEPEFFLFKKDASGRPTLELNDQGGYFDL
APVDLGENCRKEIVIELENMGFEIEASHHEVAPGQHEIDFKYADAVTTADNIQTFKLVVKTIAAKYGLHATFMPKPLFGV
NGSGMHANMSLFKGDTNVFYDPNGDMELSDTARAFTAGILEHARAFTAVCNPTVNSYKRLVPGYEAPCYVAWSARNRSPL
VRVPAARGLSTRIEVRSVDPAANPYLALAALLASGLDGIEKDLNAPKPIDSNIYVMDKPERVANGIDDLPATLFDALEVL
RADKVVMDSLGEHIGEHFLELKEIEWDMFRMQVTEWERDQYMTLY

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=438, Percent_Identity=26.9406392694064, Blast_Score=142, Evalue=6e-34,
Organism=Escherichia coli, GI1790301, Length=481, Percent_Identity=39.2931392931393, Blast_Score=294, Evalue=9e-81,
Organism=Escherichia coli, GI87081870, Length=393, Percent_Identity=27.735368956743, Blast_Score=135, Evalue=4e-33,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 50063; Mature: 49931

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARKTFTKEDIKRIAQEEDVRFIRLQFTDILGTIKNVEVPISQLDKALDNKMMFDGSSIE
CCCCCCCHHHHHHHHHHCCCEEEEEEHHHHHHHHHCCCCCHHHHHHHHCCCEEECCCCCC
GFVRIEESDMYLYPDLNTWVVFPWTDGSGKVARLICDIYNPDGTPFSGDPRGILKRNLEK
EEEEEECCCEEEECCCCEEEEEEEECCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHH
MQKLGFTAFNVGPEPEFFLFKKDASGRPTLELNDQGGYFDLAPVDLGENCRKEIVIELEN
HHHCCCEEEECCCCCCEEEEEECCCCCCEEEECCCCCEEEECCCCCCCCCCCEEEEEEEC
MGFEIEASHHEVAPGQHEIDFKYADAVTTADNIQTFKLVVKTIAAKYGLHATFMPKPLFG
CCEEEECCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCC
VNGSGMHANMSLFKGDTNVFYDPNGDMELSDTARAFTAGILEHARAFTAVCNPTVNSYKR
CCCCCCEEEEEEEECCCEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LVPGYEAPCYVAWSARNRSPLVRVPAARGLSTRIEVRSVDPAANPYLALAALLASGLDGI
HCCCCCCCEEEEECCCCCCCEEEECCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCHHH
EKDLNAPKPIDSNIYVMDKPERVANGIDDLPATLFDALEVLRADKVVMDSLGEHIGEHFL
HHHCCCCCCCCCCEEEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELKEIEWDMFRMQVTEWERDQYMTLY
HHHHCCHHHHEEEHHHCCCCCEEEEC
>Mature Secondary Structure 
ARKTFTKEDIKRIAQEEDVRFIRLQFTDILGTIKNVEVPISQLDKALDNKMMFDGSSIE
CCCCCCHHHHHHHHHHCCCEEEEEEHHHHHHHHHCCCCCHHHHHHHHCCCEEECCCCCC
GFVRIEESDMYLYPDLNTWVVFPWTDGSGKVARLICDIYNPDGTPFSGDPRGILKRNLEK
EEEEEECCCEEEECCCCEEEEEEEECCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHH
MQKLGFTAFNVGPEPEFFLFKKDASGRPTLELNDQGGYFDLAPVDLGENCRKEIVIELEN
HHHCCCEEEECCCCCCEEEEEECCCCCCEEEECCCCCEEEECCCCCCCCCCCEEEEEEEC
MGFEIEASHHEVAPGQHEIDFKYADAVTTADNIQTFKLVVKTIAAKYGLHATFMPKPLFG
CCEEEECCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCC
VNGSGMHANMSLFKGDTNVFYDPNGDMELSDTARAFTAGILEHARAFTAVCNPTVNSYKR
CCCCCCEEEEEEEECCCEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LVPGYEAPCYVAWSARNRSPLVRVPAARGLSTRIEVRSVDPAANPYLALAALLASGLDGI
HCCCCCCCEEEEECCCCCCCEEEECCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCHHH
EKDLNAPKPIDSNIYVMDKPERVANGIDDLPATLFDALEVLRADKVVMDSLGEHIGEHFL
HHHCCCCCCCCCCEEEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELKEIEWDMFRMQVTEWERDQYMTLY
HHHHCCHHHHEEEHHHCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2906311; 2565853; 9384377; 11719184 [H]