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The map label for this gene is guaA [H]

Identifier: 229916096

GI number: 229916096

Start: 326018

End: 326575

Strand: Direct

Name: guaA [H]

Synonym: EAT1b_0365

Alternate gene names: 229916096

Gene position: 326018-326575 (Clockwise)

Preceding gene: 229916094

Following gene: 229916097

Centisome position: 10.87

GC content: 47.85

Gene sequence:

>558_bases
ATGGAAGAAACAGTACGGTGTCCTTGGTGCGGGACGGATCCATTATACGTCCGTTACCATGACGAGGAATGGGGAAAGCC
CGTTCACGATGACCAGAAGCATTTTGAATGTCTGACACTCGAGAGTGCGCAAGCAGGACTTAGTTGGATCACCATCTTGA
GAAAGCGTGAAAATTATCGATTGGCTTATGCCGACTTTGAAGTGCAGCAAGTTGCCAGCTTCACGGATGAGGATGTGGAA
AGACTCCTCTCCGATAGCGGGATCGTGCGAAATCGACGAAAAATCGAGGCGTCGATCAATAATGCGAAACAGTTCATCAA
ACTACAGGAAGAGTTTGGCTCATTTGATTCGTACATCTGGTCATTCGTCAACGATGCACCGATCCTGAATGCATGGAATC
GTCTTGAAGATGTTCCTGCTACGACGGAACTTTCTGAACGATTGAGTAAAGACCTGAAACGACGCGGCTTTAAGTTTTTA
GGACCGACAACGGTCTATGCCCACCTACAAGCGACGGGTCTCGTCAATGACCATCTTACCTCTTGCACGTTCCGATAA

Upstream 100 bases:

>100_bases
AGTCACTCATGTCTCTTCCTCCATTCTCCCCCACTATACCCGTCCATTTCGCTATATAATCGTAAGGACATGCTACACTC
TAAGAAAGGAGCTGATTGAA

Downstream 100 bases:

>100_bases
CTTAAGACCAGCTCTCAAAAATATCTTTACATTCCCCCTCTTGTTGTGACAATATTTTGACAAAGTGATACTCTATCACT
GAAAGCGTTTTCATATTTTT

Product: DNA-3-methyladenine glycosylase I

Products: NA

Alternate protein names: GMP synthetase; Glutamine amidotransferase [H]

Number of amino acids: Translated: 185; Mature: 185

Protein sequence:

>185_residues
MEETVRCPWCGTDPLYVRYHDEEWGKPVHDDQKHFECLTLESAQAGLSWITILRKRENYRLAYADFEVQQVASFTDEDVE
RLLSDSGIVRNRRKIEASINNAKQFIKLQEEFGSFDSYIWSFVNDAPILNAWNRLEDVPATTELSERLSKDLKRRGFKFL
GPTTVYAHLQATGLVNDHLTSCTFR

Sequences:

>Translated_185_residues
MEETVRCPWCGTDPLYVRYHDEEWGKPVHDDQKHFECLTLESAQAGLSWITILRKRENYRLAYADFEVQQVASFTDEDVE
RLLSDSGIVRNRRKIEASINNAKQFIKLQEEFGSFDSYIWSFVNDAPILNAWNRLEDVPATTELSERLSKDLKRRGFKFL
GPTTVYAHLQATGLVNDHLTSCTFR
>Mature_185_residues
MEETVRCPWCGTDPLYVRYHDEEWGKPVHDDQKHFECLTLESAQAGLSWITILRKRENYRLAYADFEVQQVASFTDEDVE
RLLSDSGIVRNRRKIEASINNAKQFIKLQEEFGSFDSYIWSFVNDAPILNAWNRLEDVPATTELSERLSKDLKRRGFKFL
GPTTVYAHLQATGLVNDHLTSCTFR

Specific function: Catalyzes the synthesis of GMP from XMP [H]

COG id: COG2818

COG function: function code L; 3-methyladenine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 N-acetyltransferase domains [H]

Homologues:

Organism=Escherichia coli, GI1789971, Length=177, Percent_Identity=49.7175141242938, Blast_Score=199, Evalue=1e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000182
- InterPro:   IPR016181
- InterPro:   IPR005019
- InterPro:   IPR001962
- InterPro:   IPR001317
- InterPro:   IPR011257
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR001674
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00583 Acetyltransf_1; PF03352 Adenine_glyco; PF00733 Asn_synthase; PF00117 GATase; PF00958 GMP_synt_C [H]

EC number: =6.3.5.2 [H]

Molecular weight: Translated: 21524; Mature: 21524

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEETVRCPWCGTDPLYVRYHDEEWGKPVHDDQKHFECLTLESAQAGLSWITILRKRENYR
CCCCCCCCCCCCCCEEEEECCHHHCCCCCCCCHHHEEEEEHHHHHHHHHHHHHHHCCCCE
LAYADFEVQQVASFTDEDVERLLSDSGIVRNRRKIEASINNAKQFIKLQEEFGSFDSYIW
EEEECCHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
SFVNDAPILNAWNRLEDVPATTELSERLSKDLKRRGFKFLGPTTVYAHLQATGLVNDHLT
HHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCHHHEEHHHHHCCHHHHHH
SCTFR
HCCCC
>Mature Secondary Structure
MEETVRCPWCGTDPLYVRYHDEEWGKPVHDDQKHFECLTLESAQAGLSWITILRKRENYR
CCCCCCCCCCCCCCEEEEECCHHHCCCCCCCCHHHEEEEEHHHHHHHHHHHHHHHCCCCE
LAYADFEVQQVASFTDEDVERLLSDSGIVRNRRKIEASINNAKQFIKLQEEFGSFDSYIW
EEEECCHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
SFVNDAPILNAWNRLEDVPATTELSERLSKDLKRRGFKFLGPTTVYAHLQATGLVNDHLT
HHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCHHHEEHHHHHCCHHHHHH
SCTFR
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12810954 [H]