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The map label for this gene is rlmN [H]

Identifier: 229916075

GI number: 229916075

Start: 308150

End: 309211

Strand: Reverse

Name: rlmN [H]

Synonym: EAT1b_0344

Alternate gene names: 229916075

Gene position: 309211-308150 (Counterclockwise)

Preceding gene: 229916076

Following gene: 229916071

Centisome position: 10.31

GC content: 49.53

Gene sequence:

>1062_bases
ATGAAATCATCTATATACGGGTTGACGTTCGATCAACTCACAAACGAGTGTACGGAAGCGGGATACGGTGCTTTCCACGC
ACGTCAAGTTTGGGACTCGCTCTACATCGACCGGGTCAAATCGATGGACGACCTCAATGTACGTGATGAGGTGCGTGATT
ACTTAACAGAGAAATATGTCATCTCAACACAAGAACTGTTCGTCAAACAAGAAGCGGGCGACGGGACGGTCAAATTCTTA
CTTAAATTACATGACGGGCACTACATCGAGACGGTGCTCATGCGTCACAAATACGGACGTTCGGTCTGTGTGACGACTCA
GGTCGGTTGTAACATCGGCTGTAGCTTCTGTGCGAGCGGCTTGTTGAAAAAGACACGTGACTTGACGGCGGGTGAGGTCG
TCGAGCAGATCATGACCGTCCAACACTTCCTCGATGAAGAGGGTGAAGGGGACCGCGTCAGCCATATCGTCGTTATGGGA
ATCGGTGAACCGTTCGATAACTTCGACAACTTGGTCGATTTCTTACTTGTCGTCAAAGATGAGCGTGGACTTGCGATTGC
TCCGCGTAAAATCAACGTGTCGACGAGTGGTCTCGCGGATAAGATCTACAAGTTCGCAGACCTCGACTTACGCATCAACT
TGGCGCTCTCTCTACATGCGCCAAACGATGAATTACGTACTCGCATCATGAAAATCAACCGGGCGTTCCCGCTTGATAAA
CTCATGCCGTCGATTCGCTATTACGTCGAGAAGACGAATAAACGAATCACATTCGAGTATATCCTGCTCTCGAAAGTGAA
CGATTTGCCGGAACATGCGGAAGAACTCGCCGACTTGATCGAAGACATCAAAGACAAGTCGTACGTCAACTTGATTCCAT
ATAACCCGGTCAACGAGCACATCCAATATGAGCGAAGCACGCCTGAGGACATCATGGCGTTCTACGATATCTTGAAAAAA
CGTGGCGTCAACACTGGTGTCCGTCTCGAGCACGGGACAGACATCGATGCGGCATGTGGACAACTCCGCAGTAAGCATAT
GAACGTCGAAGGCGCGAAGTAA

Upstream 100 bases:

>100_bases
TCGTCACACGACCGACGCCTCCAGATTATTCGTAAGTTGCACCCGTCTCTCACGTCCGCTACAATGGTACGTATGCGGAT
TAGAGAAAGGACAGATTACA

Downstream 100 bases:

>100_bases
AAAATGGCCTCTCCGGGAAATTGAACTGAACCCTGAATGATAGACACTTGATAAAAAGTGCTCATTCAGGGTTTTTGACG
TTTCCCTTTTTGGTGGAGCA

Product: radical SAM enzyme, Cfr family

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MKSSIYGLTFDQLTNECTEAGYGAFHARQVWDSLYIDRVKSMDDLNVRDEVRDYLTEKYVISTQELFVKQEAGDGTVKFL
LKLHDGHYIETVLMRHKYGRSVCVTTQVGCNIGCSFCASGLLKKTRDLTAGEVVEQIMTVQHFLDEEGEGDRVSHIVVMG
IGEPFDNFDNLVDFLLVVKDERGLAIAPRKINVSTSGLADKIYKFADLDLRINLALSLHAPNDELRTRIMKINRAFPLDK
LMPSIRYYVEKTNKRITFEYILLSKVNDLPEHAEELADLIEDIKDKSYVNLIPYNPVNEHIQYERSTPEDIMAFYDILKK
RGVNTGVRLEHGTDIDAACGQLRSKHMNVEGAK

Sequences:

>Translated_353_residues
MKSSIYGLTFDQLTNECTEAGYGAFHARQVWDSLYIDRVKSMDDLNVRDEVRDYLTEKYVISTQELFVKQEAGDGTVKFL
LKLHDGHYIETVLMRHKYGRSVCVTTQVGCNIGCSFCASGLLKKTRDLTAGEVVEQIMTVQHFLDEEGEGDRVSHIVVMG
IGEPFDNFDNLVDFLLVVKDERGLAIAPRKINVSTSGLADKIYKFADLDLRINLALSLHAPNDELRTRIMKINRAFPLDK
LMPSIRYYVEKTNKRITFEYILLSKVNDLPEHAEELADLIEDIKDKSYVNLIPYNPVNEHIQYERSTPEDIMAFYDILKK
RGVNTGVRLEHGTDIDAACGQLRSKHMNVEGAK
>Mature_353_residues
MKSSIYGLTFDQLTNECTEAGYGAFHARQVWDSLYIDRVKSMDDLNVRDEVRDYLTEKYVISTQELFVKQEAGDGTVKFL
LKLHDGHYIETVLMRHKYGRSVCVTTQVGCNIGCSFCASGLLKKTRDLTAGEVVEQIMTVQHFLDEEGEGDRVSHIVVMG
IGEPFDNFDNLVDFLLVVKDERGLAIAPRKINVSTSGLADKIYKFADLDLRINLALSLHAPNDELRTRIMKINRAFPLDK
LMPSIRYYVEKTNKRITFEYILLSKVNDLPEHAEELADLIEDIKDKSYVNLIPYNPVNEHIQYERSTPEDIMAFYDILKK
RGVNTGVRLEHGTDIDAACGQLRSKHMNVEGAK

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=349, Percent_Identity=34.6704871060172, Blast_Score=201, Evalue=6e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 40193; Mature: 40193

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSSIYGLTFDQLTNECTEAGYGAFHARQVWDSLYIDRVKSMDDLNVRDEVRDYLTEKYV
CCCCEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHE
ISTQELFVKQEAGDGTVKFLLKLHDGHYIETVLMRHKYGRSVCVTTQVGCNIGCSFCASG
EEHHHHHEEECCCCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHH
LLKKTRDLTAGEVVEQIMTVQHFLDEEGEGDRVSHIVVMGIGEPFDNFDNLVDFLLVVKD
HHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHEEC
ERGLAIAPRKINVSTSGLADKIYKFADLDLRINLALSLHAPNDELRTRIMKINRAFPLDK
CCCCEECCCEECCCCHHHHHHHHHHHCCEEEEEEEEEEECCCHHHHHHHHHHHCCCCHHH
LMPSIRYYVEKTNKRITFEYILLSKVNDLPEHAEELADLIEDIKDKSYVNLIPYNPVNEH
HHHHHHHHHHHCCCEEEEEEEEEHHHCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHH
IQYERSTPEDIMAFYDILKKRGVNTGVRLEHGTDIDAACGQLRSKHMNVEGAK
HEECCCCHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKSSIYGLTFDQLTNECTEAGYGAFHARQVWDSLYIDRVKSMDDLNVRDEVRDYLTEKYV
CCCCEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHE
ISTQELFVKQEAGDGTVKFLLKLHDGHYIETVLMRHKYGRSVCVTTQVGCNIGCSFCASG
EEHHHHHEEECCCCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHH
LLKKTRDLTAGEVVEQIMTVQHFLDEEGEGDRVSHIVVMGIGEPFDNFDNLVDFLLVVKD
HHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHEEC
ERGLAIAPRKINVSTSGLADKIYKFADLDLRINLALSLHAPNDELRTRIMKINRAFPLDK
CCCCEECCCEECCCCHHHHHHHHHHHCCEEEEEEEEEEECCCHHHHHHHHHHHCCCCHHH
LMPSIRYYVEKTNKRITFEYILLSKVNDLPEHAEELADLIEDIKDKSYVNLIPYNPVNEH
HHHHHHHHHHHCCCEEEEEEEEEHHHCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHH
IQYERSTPEDIMAFYDILKKRGVNTGVRLEHGTDIDAACGQLRSKHMNVEGAK
HEECCCCHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA