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The map label for this gene is gldA [H]

Identifier: 229916027

GI number: 229916027

Start: 262207

End: 263223

Strand: Reverse

Name: gldA [H]

Synonym: EAT1b_0295

Alternate gene names: 229916027

Gene position: 263223-262207 (Counterclockwise)

Preceding gene: 229916028

Following gene: 229916026

Centisome position: 8.77

GC content: 48.87

Gene sequence:

>1017_bases
ATGATTCGACAAGCGGTGCTGGAATATGACCATTCGAACGATGCGCTCGATCAACTTTACTCATTGATTGGGAATCGGGC
GGCACTCGTTTTGAAAGGATTTAACGGATACGGTGAAGCGATTCGCGTGTCCCCTCGTCTAGCAGAAATTTCGAGCGTAC
GATTCAAGGGGGAATGTTCAGACGAAGAAGTCGAACGACTAACAGCTTATCGACCTGACGTATGGATTGCCGTCGGTGGC
GGTAAATTGATTGATACGGCGAAGCGGATTGCATTCACTCAGTCGGTCCCGCTCATCGTCGTTCCGACACTTGTGTCGAA
TTGTGCCACGTTTACCCCGCTCAGCGTGATCTATGGTGAAGATGGTACATATTTACGATACGATACGTTCCCTGTCATCA
TCGAGCGCGTCATTGCGGATGAACGACTGTTACGTGATTCACCTTACGATTATTTTAAGGCTGGTGTCATCGATACGGTC
GCAAAGTGGTTCGAGGCTCGATATATTAGCGGAACAACATCAAGGGACGCAGGGATTCAGTCGGGGCTTTCGCTTGCGGC
ACAATGCGGAGCGTTGATGGATTTGTCCATTCGTGAAGATGATTTTCGCCATTATGGCACTGACATCCGATATATCGTTG
ATCATGTCCTCGCCATCGGAGGTGCCGTCGGTGGGTTCGGCGATGCCGGCACTCGTGTCGCCGTCGCCCATGCGGTGCAT
AATGCGCTCAGTCGTTTTGATTCGACGCATCACTGGTTGCACGGGGATAAAGTAGGCTTTGGGCTGATCGTTCAAGAACG
ATTGGCTTCATCCGAAGACGCCTCATTCCTCGAGAATTGGCTACACGCCATCGCCGCTCCAACGTCACTCGAACAACTTG
GCATTCGCTTTGATCAAGTCGATGAATTGGTCGACTATGTCATCGAAGAAATGTCGATTCAAGGCATACGCAAAATCGAA
GATGCTGAACGATTAAAAGAAATTTTAAGAAATCTTCAAAAAATGGTTTACAAATAA

Upstream 100 bases:

>100_bases
AGCACCACATCCGCTTTGGTCTCGTGTCGTCTGAACAGAAGATTGATGAAGCGGTAGAACGAATTGAGAGACTGTTTGCA
GCCTACGAGGAGATTCATCA

Downstream 100 bases:

>100_bases
TTCTTTTATCAGTAATATAATAACAAAATAATAGAACCGATTGCTTATCAAGAGTGGTGGAGGGACTGGCCCGATGAAAC
CCGGCAACCAGATCAGAAAT

Product: iron-containing alcohol dehydrogenase

Products: NA

Alternate protein names: GDH; GLDH [H]

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MIRQAVLEYDHSNDALDQLYSLIGNRAALVLKGFNGYGEAIRVSPRLAEISSVRFKGECSDEEVERLTAYRPDVWIAVGG
GKLIDTAKRIAFTQSVPLIVVPTLVSNCATFTPLSVIYGEDGTYLRYDTFPVIIERVIADERLLRDSPYDYFKAGVIDTV
AKWFEARYISGTTSRDAGIQSGLSLAAQCGALMDLSIREDDFRHYGTDIRYIVDHVLAIGGAVGGFGDAGTRVAVAHAVH
NALSRFDSTHHWLHGDKVGFGLIVQERLASSEDASFLENWLHAIAAPTSLEQLGIRFDQVDELVDYVIEEMSIQGIRKIE
DAERLKEILRNLQKMVYK

Sequences:

>Translated_338_residues
MIRQAVLEYDHSNDALDQLYSLIGNRAALVLKGFNGYGEAIRVSPRLAEISSVRFKGECSDEEVERLTAYRPDVWIAVGG
GKLIDTAKRIAFTQSVPLIVVPTLVSNCATFTPLSVIYGEDGTYLRYDTFPVIIERVIADERLLRDSPYDYFKAGVIDTV
AKWFEARYISGTTSRDAGIQSGLSLAAQCGALMDLSIREDDFRHYGTDIRYIVDHVLAIGGAVGGFGDAGTRVAVAHAVH
NALSRFDSTHHWLHGDKVGFGLIVQERLASSEDASFLENWLHAIAAPTSLEQLGIRFDQVDELVDYVIEEMSIQGIRKIE
DAERLKEILRNLQKMVYK
>Mature_338_residues
MIRQAVLEYDHSNDALDQLYSLIGNRAALVLKGFNGYGEAIRVSPRLAEISSVRFKGECSDEEVERLTAYRPDVWIAVGG
GKLIDTAKRIAFTQSVPLIVVPTLVSNCATFTPLSVIYGEDGTYLRYDTFPVIIERVIADERLLRDSPYDYFKAGVIDTV
AKWFEARYISGTTSRDAGIQSGLSLAAQCGALMDLSIREDDFRHYGTDIRYIVDHVLAIGGAVGGFGDAGTRVAVAHAVH
NALSRFDSTHHWLHGDKVGFGLIVQERLASSEDASFLENWLHAIAAPTSLEQLGIRFDQVDELVDYVIEEMSIQGIRKIE
DAERLKEILRNLQKMVYK

Specific function: Catalyzes the NAD-dependent oxidation of glycerol to dihydroxyacetone (glycerone). Allows microorganisms to utilize glycerol as a source of carbon under anaerobic conditions [H]

COG id: COG0371

COG function: function code C; Glycerol dehydrogenase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI87082352, Length=290, Percent_Identity=31.7241379310345, Blast_Score=134, Evalue=7e-33,
Organism=Escherichia coli, GI1786815, Length=286, Percent_Identity=33.2167832167832, Blast_Score=132, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211
- InterPro:   IPR016205 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: =1.1.1.6 [H]

Molecular weight: Translated: 37577; Mature: 37577

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRQAVLEYDHSNDALDQLYSLIGNRAALVLKGFNGYGEAIRVSPRLAEISSVRFKGECS
CCCHHHHHCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCEEECCCHHHHHHHCEECCCCC
DEEVERLTAYRPDVWIAVGGGKLIDTAKRIAFTQSVPLIVVPTLVSNCATFTPLSVIYGE
HHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEHHHHHHCCHHCCCEEEEECC
DGTYLRYDTFPVIIERVIADERLLRDSPYDYFKAGVIDTVAKWFEARYISGTTSRDAGIQ
CCCEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
SGLSLAAQCGALMDLSIREDDFRHYGTDIRYIVDHVLAIGGAVGGFGDAGTRVAVAHAVH
HHHHHHHHCCHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
NALSRFDSTHHWLHGDKVGFGLIVQERLASSEDASFLENWLHAIAAPTSLEQLGIRFDQV
HHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHCCCHHHH
DELVDYVIEEMSIQGIRKIEDAERLKEILRNLQKMVYK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIRQAVLEYDHSNDALDQLYSLIGNRAALVLKGFNGYGEAIRVSPRLAEISSVRFKGECS
CCCHHHHHCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCEEECCCHHHHHHHCEECCCCC
DEEVERLTAYRPDVWIAVGGGKLIDTAKRIAFTQSVPLIVVPTLVSNCATFTPLSVIYGE
HHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEHHHHHHCCHHCCCEEEEECC
DGTYLRYDTFPVIIERVIADERLLRDSPYDYFKAGVIDTVAKWFEARYISGTTSRDAGIQ
CCCEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
SGLSLAAQCGALMDLSIREDDFRHYGTDIRYIVDHVLAIGGAVGGFGDAGTRVAVAHAVH
HHHHHHHHCCHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
NALSRFDSTHHWLHGDKVGFGLIVQERLASSEDASFLENWLHAIAAPTSLEQLGIRFDQV
HHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHCCCHHHH
DELVDYVIEEMSIQGIRKIEDAERLKEILRNLQKMVYK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571; 12193646 [H]