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The map label for this gene is mtnE [H]

Identifier: 229916028

GI number: 229916028

Start: 263220

End: 264395

Strand: Reverse

Name: mtnE [H]

Synonym: EAT1b_0296

Alternate gene names: 229916028

Gene position: 264395-263220 (Counterclockwise)

Preceding gene: 229916032

Following gene: 229916027

Centisome position: 8.81

GC content: 52.81

Gene sequence:

>1176_bases
ATGGCAGACAGACGTTCCAAGTTATTAAAATCGTTACCGACACAACACTTTGTCGGATTAGCAGAAGAAGTCGCGGCAGT
CGAGGCGAGTGGCAAGACCGTCATCCGACTCGGACAAGGCACACCCGACTTACCGACACCAGCTCCAATCTGTGAGGCAC
TGGAACGAGCATTGACCGACCCGACGACCCATCAATACGGACCATTCCGGGGACAGGCTCGACTAAAAGAAGCTGTGGCC
GCATTCTATCAACGTGAATACGGAGTCGACCTCGACCCAGCAAAGGAAGTGGCGATCTTGATTGGTAGCAAATCGGGTCT
AATCACGTTGCCTCAATGTCTTCTTGAACCAGGTGAAGGCATCTTGCTCCCGAACCCTGGCTATCCCGATTATATCTCGG
GCGCGCGCCTCGCCGGAGCGGACATCATCGATTTGACGTTAAAGGAGGAACACGGCTATCTCCCGGATTACGCCACGCTC
GATCCGCTTCATGCTCGACTCATGTATTTGAACTACCCGAGCAATCCGACCGGCGCTGTCGCGACGCCAGCCTTCTTTGA
GGAGACGGTTAAATTCGCCACAAACCATGATCTGATGGTCGTACACGATTTCGCCTATGGAAGTATTGGCTTTGATGGGC
ATGTCCCGCCAAGTTTTCTCCAAGCAGACGGGGCAAAAGAAATCGGGATTGAGGTGTATACGATGTCGAAGGCGTTCAAC
ATGTCGGGATGGCGTGTCGCGTTCGCAGTCGGAAATGCAGATATCATCGAGGCGATCAATACGTACCAAGATCATGTCCA
CGTCTCTGTGTTCTCTGCCATTCAAGAGGCAGCCATCGTGGCGTTAGAATCCCCGCGTGACATACGTGACGACCTAGCCT
CGCTTTACGAAACAAGACGAAACCGATTGATTGATGGGTTACGCCGGGCAGGGTGGGAGATTGAGGCACCAAAAGGTTCT
TTCTTCGTATGGGCGAGAGTGCCTGAAGGCGATGCAAAATCATTCTCGACGAAGTTACTACATGAGGCGGGCGTCGCCGT
CACACCGGGCTACTTTTTTGGAACGGAGGGGGAGCACCACATCCGCTTTGGTCTCGTGTCGTCTGAACAGAAGATTGATG
AAGCGGTAGAACGAATTGAGAGACTGTTTGCAGCCTACGAGGAGATTCATCAATGA

Upstream 100 bases:

>100_bases
AGGTGACCTTGAGAGATGGAAAGGTGGTTGACTACATACGTCTGTATCGAGCACCCCTTTCCTCAGAAAGGGGTGTTTTT
TCGTATGAGGAGGACCCATC

Downstream 100 bases:

>100_bases
TTCGACAAGCGGTGCTGGAATATGACCATTCGAACGATGCGCTCGATCAACTTTACTCATTGATTGGGAATCGGGCGGCA
CTCGTTTTGAAAGGATTTAA

Product: aminotransferase class I and II

Products: N-succinyl-2-L-amino-6-oxoheptanedioate; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MADRRSKLLKSLPTQHFVGLAEEVAAVEASGKTVIRLGQGTPDLPTPAPICEALERALTDPTTHQYGPFRGQARLKEAVA
AFYQREYGVDLDPAKEVAILIGSKSGLITLPQCLLEPGEGILLPNPGYPDYISGARLAGADIIDLTLKEEHGYLPDYATL
DPLHARLMYLNYPSNPTGAVATPAFFEETVKFATNHDLMVVHDFAYGSIGFDGHVPPSFLQADGAKEIGIEVYTMSKAFN
MSGWRVAFAVGNADIIEAINTYQDHVHVSVFSAIQEAAIVALESPRDIRDDLASLYETRRNRLIDGLRRAGWEIEAPKGS
FFVWARVPEGDAKSFSTKLLHEAGVAVTPGYFFGTEGEHHIRFGLVSSEQKIDEAVERIERLFAAYEEIHQ

Sequences:

>Translated_391_residues
MADRRSKLLKSLPTQHFVGLAEEVAAVEASGKTVIRLGQGTPDLPTPAPICEALERALTDPTTHQYGPFRGQARLKEAVA
AFYQREYGVDLDPAKEVAILIGSKSGLITLPQCLLEPGEGILLPNPGYPDYISGARLAGADIIDLTLKEEHGYLPDYATL
DPLHARLMYLNYPSNPTGAVATPAFFEETVKFATNHDLMVVHDFAYGSIGFDGHVPPSFLQADGAKEIGIEVYTMSKAFN
MSGWRVAFAVGNADIIEAINTYQDHVHVSVFSAIQEAAIVALESPRDIRDDLASLYETRRNRLIDGLRRAGWEIEAPKGS
FFVWARVPEGDAKSFSTKLLHEAGVAVTPGYFFGTEGEHHIRFGLVSSEQKIDEAVERIERLFAAYEEIHQ
>Mature_390_residues
ADRRSKLLKSLPTQHFVGLAEEVAAVEASGKTVIRLGQGTPDLPTPAPICEALERALTDPTTHQYGPFRGQARLKEAVAA
FYQREYGVDLDPAKEVAILIGSKSGLITLPQCLLEPGEGILLPNPGYPDYISGARLAGADIIDLTLKEEHGYLPDYATLD
PLHARLMYLNYPSNPTGAVATPAFFEETVKFATNHDLMVVHDFAYGSIGFDGHVPPSFLQADGAKEIGIEVYTMSKAFNM
SGWRVAFAVGNADIIEAINTYQDHVHVSVFSAIQEAAIVALESPRDIRDDLASLYETRRNRLIDGLRRAGWEIEAPKGSF
FVWARVPEGDAKSFSTKLLHEAGVAVTPGYFFGTEGEHHIRFGLVSSEQKIDEAVERIERLFAAYEEIHQ

Specific function: Catalyzes the formation of methionine from 2-keto-4- methylthiobutyrate (KMTB) (Probable) [H]

COG id: COG0436

COG function: function code E; Aspartate/tyrosine/aromatic aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. MtnE subfamily [H]

Homologues:

Organism=Homo sapiens, GI56713256, Length=388, Percent_Identity=23.9690721649485, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI56713254, Length=388, Percent_Identity=23.9690721649485, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI95147551, Length=421, Percent_Identity=24.2280285035629, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI169881279, Length=421, Percent_Identity=24.2280285035629, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI4507369, Length=228, Percent_Identity=25.4385964912281, Blast_Score=66, Evalue=7e-11,
Organism=Escherichia coli, GI1788722, Length=390, Percent_Identity=38.4615384615385, Blast_Score=281, Evalue=5e-77,
Organism=Escherichia coli, GI1786816, Length=361, Percent_Identity=26.5927977839335, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1788627, Length=397, Percent_Identity=23.9294710327456, Blast_Score=82, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71994472, Length=408, Percent_Identity=24.2647058823529, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71994476, Length=408, Percent_Identity=24.2647058823529, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17567369, Length=398, Percent_Identity=23.6180904522613, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17567663, Length=394, Percent_Identity=25.6345177664975, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6322401, Length=398, Percent_Identity=23.6180904522613, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI28573069, Length=385, Percent_Identity=21.5584415584416, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24646114, Length=385, Percent_Identity=21.5584415584416, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI28573067, Length=385, Percent_Identity=21.5584415584416, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI28573065, Length=385, Percent_Identity=21.5584415584416, Blast_Score=92, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004839
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: 2.6.1.17

Molecular weight: Translated: 42913; Mature: 42782

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADRRSKLLKSLPTQHFVGLAEEVAAVEASGKTVIRLGQGTPDLPTPAPICEALERALTD
CCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCC
PTTHQYGPFRGQARLKEAVAAFYQREYGVDLDPAKEVAILIGSKSGLITLPQCLLEPGEG
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEECHHHHCCCCCE
ILLPNPGYPDYISGARLAGADIIDLTLKEEHGYLPDYATLDPLHARLMYLNYPSNPTGAV
EEECCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEEEECCCCCCCCC
ATPAFFEETVKFATNHDLMVVHDFAYGSIGFDGHVPPSFLQADGAKEIGIEVYTMSKAFN
CCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHCCCCCCCCEEEEEECCCCC
MSGWRVAFAVGNADIIEAINTYQDHVHVSVFSAIQEAAIVALESPRDIRDDLASLYETRR
CCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHH
NRLIDGLRRAGWEIEAPKGSFFVWARVPEGDAKSFSTKLLHEAGVAVTPGYFFGTEGEHH
HHHHHHHHHCCCEEECCCCCEEEEEECCCCCHHHHHHHHHHHCCCEECCCEEECCCCCCE
IRFGLVSSEQKIDEAVERIERLFAAYEEIHQ
EEEEECCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ADRRSKLLKSLPTQHFVGLAEEVAAVEASGKTVIRLGQGTPDLPTPAPICEALERALTD
CCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCC
PTTHQYGPFRGQARLKEAVAAFYQREYGVDLDPAKEVAILIGSKSGLITLPQCLLEPGEG
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEECHHHHCCCCCE
ILLPNPGYPDYISGARLAGADIIDLTLKEEHGYLPDYATLDPLHARLMYLNYPSNPTGAV
EEECCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEEEECCCCCCCCC
ATPAFFEETVKFATNHDLMVVHDFAYGSIGFDGHVPPSFLQADGAKEIGIEVYTMSKAFN
CCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHCCCCCCCCEEEEEECCCCC
MSGWRVAFAVGNADIIEAINTYQDHVHVSVFSAIQEAAIVALESPRDIRDDLASLYETRR
CCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHH
NRLIDGLRRAGWEIEAPKGSFFVWARVPEGDAKSFSTKLLHEAGVAVTPGYFFGTEGEHH
HHHHHHHHHCCCEEECCCCCEEEEEECCCCCHHHHHHHHHHHCCCEECCCEEECCCCCCE
IRFGLVSSEQKIDEAVERIERLFAAYEEIHQ
EEEEECCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-succinyl-L-2,6-diaminoheptanedioate; 2-oxoglutarate

Specific reaction: N-succinyl-L-2,6-diaminoheptanedioate + 2-oxoglutarate = N-succinyl-2-L-amino-6-oxoheptanedioate + L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 12022921 [H]