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The map label for this gene is pleD [H]

Identifier: 229916012

GI number: 229916012

Start: 247001

End: 248044

Strand: Direct

Name: pleD [H]

Synonym: EAT1b_0280

Alternate gene names: 229916012

Gene position: 247001-248044 (Clockwise)

Preceding gene: 229916008

Following gene: 229916013

Centisome position: 8.23

GC content: 47.99

Gene sequence:

>1044_bases
ATGGGAACAACCTATGAAACTCGTCGAGGGACACTATATAACGAACGGATTACTTTTTACGGCAATGTCATCGCCTGGTT
GGTCCACTTCCTATTTTGCATCTTGTTTTATCGTTTGGATATGACTCTCTTATTCCTATCAAATGGCTTGAGTGTGATCT
ATTACACGATTTCATTCTACTTTGTACATAGACGGTGGTACGGCATCTTCTTTACCGGACTTTTTATCGAAGTCGTTTAC
AACGCCGTCATCTCGACCGTCATTCTCGGTTGGGAGATCAACATGCATTATTTCATCCTCATGATGGCCTATGGTGTCTT
TTTCATGCCAAGCGTATCCAGAGGCCAACGCATCTTGACCACGGCCATCGCGATGTTGATTTATAGCCTTCTATTCATCT
ATGTGTCCGATGGCATCGAGACCCTACCGATTCAGGTGGAACAAGCGCTCGGGCTCTTTTGCATCCTATCGACCGTGGTG
ATGATCGCAGGGCTCTCCTTCATCTTCGAGTCAGCCGTTGTCGAAGTGACAGATGAACTCGAACGATCCAATGAGCGATT
GCAAATCCTAGCGTCTACAGATGGATTGACCGGACTATTGAACCGAAAAGTTGGGCATGAACACGTTTCTAAAGCAATCG
ACAAAGCCGCTCATGAAGGCGTTCCGTATGCAATCGTTCTCGGTGACATCGATCGATTCAAATCGTTTAACGAGACGTAC
GGTCATGATTGTGGGGACCGGGTGCTTCAACAAGTGGCCTCCATCTTATCCGATATCGACGGCATGGCCGTCCGCTGGGG
AGGAGAGGAGTTTCTGGTCTACCTATCGAGTCCGTCCGGTGACTTCCTTCCAATCGTCGAGCAGTTGAGACAACAAATCG
AGTCGACCCCTCTCCATTACGCAGGAGAACGTCTTCATGTCACAATGACATTCGGACTTGCGGTTCGTGTTGGTCACGAT
ACGGTCGAAGAGATCGTCAAAGAAGCGGACAACTTCCTTCGTGAAGGGAAGCGTAGCGGAAAGAATCAAGTGAGAACTAC
ATGA

Upstream 100 bases:

>100_bases
AACGATACATACGAAATTAGGACGTTTTTTAAGGTCATTTTACGGACTTCTATACAAATTTAATCATTGTTTCGTTATAC
TAAAAGAAAGGAGGTGTGTG

Downstream 100 bases:

>100_bases
AGAAAGGGGCATTCTATGAGTATTTTCCGTCAACTTGGACAGCGGCAGACACCGACGACGACGAAACGCTTCGAGTTGAA
CGGGTTGCACCGACTACCCG

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 347; Mature: 346

Protein sequence:

>347_residues
MGTTYETRRGTLYNERITFYGNVIAWLVHFLFCILFYRLDMTLLFLSNGLSVIYYTISFYFVHRRWYGIFFTGLFIEVVY
NAVISTVILGWEINMHYFILMMAYGVFFMPSVSRGQRILTTAIAMLIYSLLFIYVSDGIETLPIQVEQALGLFCILSTVV
MIAGLSFIFESAVVEVTDELERSNERLQILASTDGLTGLLNRKVGHEHVSKAIDKAAHEGVPYAIVLGDIDRFKSFNETY
GHDCGDRVLQQVASILSDIDGMAVRWGGEEFLVYLSSPSGDFLPIVEQLRQQIESTPLHYAGERLHVTMTFGLAVRVGHD
TVEEIVKEADNFLREGKRSGKNQVRTT

Sequences:

>Translated_347_residues
MGTTYETRRGTLYNERITFYGNVIAWLVHFLFCILFYRLDMTLLFLSNGLSVIYYTISFYFVHRRWYGIFFTGLFIEVVY
NAVISTVILGWEINMHYFILMMAYGVFFMPSVSRGQRILTTAIAMLIYSLLFIYVSDGIETLPIQVEQALGLFCILSTVV
MIAGLSFIFESAVVEVTDELERSNERLQILASTDGLTGLLNRKVGHEHVSKAIDKAAHEGVPYAIVLGDIDRFKSFNETY
GHDCGDRVLQQVASILSDIDGMAVRWGGEEFLVYLSSPSGDFLPIVEQLRQQIESTPLHYAGERLHVTMTFGLAVRVGHD
TVEEIVKEADNFLREGKRSGKNQVRTT
>Mature_346_residues
GTTYETRRGTLYNERITFYGNVIAWLVHFLFCILFYRLDMTLLFLSNGLSVIYYTISFYFVHRRWYGIFFTGLFIEVVYN
AVISTVILGWEINMHYFILMMAYGVFFMPSVSRGQRILTTAIAMLIYSLLFIYVSDGIETLPIQVEQALGLFCILSTVVM
IAGLSFIFESAVVEVTDELERSNERLQILASTDGLTGLLNRKVGHEHVSKAIDKAAHEGVPYAIVLGDIDRFKSFNETYG
HDCGDRVLQQVASILSDIDGMAVRWGGEEFLVYLSSPSGDFLPIVEQLRQQIESTPLHYAGERLHVTMTFGLAVRVGHDT
VEEIVKEADNFLREGKRSGKNQVRTT

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=156, Percent_Identity=33.974358974359, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87081881, Length=185, Percent_Identity=30.8108108108108, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1787802, Length=278, Percent_Identity=25.8992805755396, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787262, Length=164, Percent_Identity=31.0975609756098, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787541, Length=179, Percent_Identity=33.5195530726257, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1786584, Length=247, Percent_Identity=24.2914979757085, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1787816, Length=167, Percent_Identity=30.5389221556886, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI87082007, Length=165, Percent_Identity=29.0909090909091, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI87081977, Length=173, Percent_Identity=33.5260115606936, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1788956, Length=164, Percent_Identity=29.8780487804878, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787056, Length=156, Percent_Identity=28.8461538461538, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788085, Length=187, Percent_Identity=27.807486631016, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI87081974, Length=156, Percent_Identity=26.9230769230769, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 39394; Mature: 39263

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTTYETRRGTLYNERITFYGNVIAWLVHFLFCILFYRLDMTLLFLSNGLSVIYYTISFY
CCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
FVHRRWYGIFFTGLFIEVVYNAVISTVILGWEINMHYFILMMAYGVFFMPSVSRGQRILT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEHHHHHHHHHHHHHHHCCCCCCCHHHHH
TAIAMLIYSLLFIYVSDGIETLPIQVEQALGLFCILSTVVMIAGLSFIFESAVVEVTDEL
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERSNERLQILASTDGLTGLLNRKVGHEHVSKAIDKAAHEGVPYAIVLGDIDRFKSFNETY
HCCCCCEEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCHHHHHCCCHHC
GHDCGDRVLQQVASILSDIDGMAVRWGGEEFLVYLSSPSGDFLPIVEQLRQQIESTPLHY
CCCHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCC
AGERLHVTMTFGLAVRVGHDTVEEIVKEADNFLREGKRSGKNQVRTT
CCCEEEEEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
GTTYETRRGTLYNERITFYGNVIAWLVHFLFCILFYRLDMTLLFLSNGLSVIYYTISFY
CCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
FVHRRWYGIFFTGLFIEVVYNAVISTVILGWEINMHYFILMMAYGVFFMPSVSRGQRILT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEHHHHHHHHHHHHHHHCCCCCCCHHHHH
TAIAMLIYSLLFIYVSDGIETLPIQVEQALGLFCILSTVVMIAGLSFIFESAVVEVTDEL
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERSNERLQILASTDGLTGLLNRKVGHEHVSKAIDKAAHEGVPYAIVLGDIDRFKSFNETY
HCCCCCEEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCHHHHHCCCHHC
GHDCGDRVLQQVASILSDIDGMAVRWGGEEFLVYLSSPSGDFLPIVEQLRQQIESTPLHY
CCCHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCC
AGERLHVTMTFGLAVRVGHDTVEEIVKEADNFLREGKRSGKNQVRTT
CCCEEEEEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA