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The map label for this gene is thyA

Identifier: 229915874

GI number: 229915874

Start: 113927

End: 114721

Strand: Reverse

Name: thyA

Synonym: EAT1b_0141

Alternate gene names: 229915874

Gene position: 114721-113927 (Counterclockwise)

Preceding gene: 229915878

Following gene: 229915873

Centisome position: 3.82

GC content: 47.92

Gene sequence:

>795_bases
ATGAAACAATATCATGACTTATGTCGACATATCCTCGACCAAGGTGTCGTAAAAGAAGACCGGACCGGGACCGGGACAAC
GAGTGTATTTGGTTACCAAATGCGTTTCAATTTACAAGAAGGGTTCCCCTTGATCACGACGAAAAAATTACATATCCGTT
CCATCATTCACGAACTATTATGGTTCATCTCAGGAGAGACGAACGTCCGATACCTTCAAGATAATGGGGTCCGCATTTGG
AACGAATGGGCAGACGAAGAAGGCAATCTTGGTCCAGTATACGGTTCACAGTGGCGTTCATTCCCACGCCCGGACGGCTC
GTCCGTCGATCAACTGGCTCAAGTGATTGAACAAATCAAGACGAATCCAGATTCGAGACGATTGATCGTGTCGGCATGGA
ACCCAGGACAGCTCGAAGAGATGGCCCTCCCGCCGTGTCATTTACTTTTCCAATTCTATGTCGCGGACGGAAAGCTTTCG
TGCCAGTTATATCAACGAAGCGCCGACACGTTTTTAGGTGTTCCATTCAACATCGCATCGTATGCACTTTTGACGCATAT
GGTCGCACACGTGACCGGACTTGAAGTCGGCGACTTTGTCCATACCCTTGGTGATGCACACATCTACCACAACCATCTCG
AGCAAGTGGAGCTTCAACTGTCGCGTGACCCGCGTCCGCTACCAAAACTCAACATTGTCCGTGACGTATCTTCAATTGAA
TCATTCCGTTTTGAAGACTTCGAAATTCTCGGTTATGACCCGCATCCACATATTAAAGGAGAAGTGAGCGTATGA

Upstream 100 bases:

>100_bases
AGGATATTGATTATGGATGATTCTCACCACTATATCTTGTGTTTCCTCCCTACAATTGATACCTTTAGTGGTAGGAGATT
GAGAAGGAGGCACACTACGT

Downstream 100 bases:

>100_bases
TTTCACACGTCGTCGCATATGGGCAGAATCGCGAAATCGGAAAAGACAATGATTTATTATGGCGCTTACCAGATGACTTG
AAACATTTCAAGGCCATCAC

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKQYHDLCRHILDQGVVKEDRTGTGTTSVFGYQMRFNLQEGFPLITTKKLHIRSIIHELLWFISGETNVRYLQDNGVRIW
NEWADEEGNLGPVYGSQWRSFPRPDGSSVDQLAQVIEQIKTNPDSRRLIVSAWNPGQLEEMALPPCHLLFQFYVADGKLS
CQLYQRSADTFLGVPFNIASYALLTHMVAHVTGLEVGDFVHTLGDAHIYHNHLEQVELQLSRDPRPLPKLNIVRDVSSIE
SFRFEDFEILGYDPHPHIKGEVSV

Sequences:

>Translated_264_residues
MKQYHDLCRHILDQGVVKEDRTGTGTTSVFGYQMRFNLQEGFPLITTKKLHIRSIIHELLWFISGETNVRYLQDNGVRIW
NEWADEEGNLGPVYGSQWRSFPRPDGSSVDQLAQVIEQIKTNPDSRRLIVSAWNPGQLEEMALPPCHLLFQFYVADGKLS
CQLYQRSADTFLGVPFNIASYALLTHMVAHVTGLEVGDFVHTLGDAHIYHNHLEQVELQLSRDPRPLPKLNIVRDVSSIE
SFRFEDFEILGYDPHPHIKGEVSV
>Mature_264_residues
MKQYHDLCRHILDQGVVKEDRTGTGTTSVFGYQMRFNLQEGFPLITTKKLHIRSIIHELLWFISGETNVRYLQDNGVRIW
NEWADEEGNLGPVYGSQWRSFPRPDGSSVDQLAQVIEQIKTNPDSRRLIVSAWNPGQLEEMALPPCHLLFQFYVADGKLS
CQLYQRSADTFLGVPFNIASYALLTHMVAHVTGLEVGDFVHTLGDAHIYHNHLEQVELQLSRDPRPLPKLNIVRDVSSIE
SFRFEDFEILGYDPHPHIKGEVSV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily

Homologues:

Organism=Homo sapiens, GI4507751, Length=282, Percent_Identity=56.7375886524823, Blast_Score=325, Evalue=3e-89,
Organism=Escherichia coli, GI1789191, Length=264, Percent_Identity=67.4242424242424, Blast_Score=390, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI71993377, Length=283, Percent_Identity=49.469964664311, Blast_Score=285, Evalue=2e-77,
Organism=Saccharomyces cerevisiae, GI6324648, Length=280, Percent_Identity=45, Blast_Score=231, Evalue=6e-62,
Organism=Drosophila melanogaster, GI17137556, Length=283, Percent_Identity=54.7703180212014, Blast_Score=315, Evalue=1e-86,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): TYSY_EXISA (C4L1C9)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002884520.1
- GeneID:   7870221
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_0141
- OMA:   RMALAPC
- ProtClustDB:   CLSK2489404
- GO:   GO:0005737
- HAMAP:   MF_00008
- InterPro:   IPR000398
- InterPro:   IPR020940
- Gene3D:   G3DSA:3.30.572.10
- PANTHER:   PTHR11549:SF2
- PRINTS:   PR00108
- TIGRFAMs:   TIGR03284

Pfam domain/function: PF00303 Thymidylat_synt; SSF55831 Thymidylat_synth_C

EC number: =2.1.1.45

Molecular weight: Translated: 30226; Mature: 30226

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: ACT_SITE 146-146

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQYHDLCRHILDQGVVKEDRTGTGTTSVFGYQMRFNLQEGFPLITTKKLHIRSIIHELL
CCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEECCCCCCEEEECHHHHHHHHHHHH
WFISGETNVRYLQDNGVRIWNEWADEEGNLGPVYGSQWRSFPRPDGSSVDQLAQVIEQIK
HHHCCCCCEEEEECCCEEEEECCCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHH
TNPDSRRLIVSAWNPGQLEEMALPPCHLLFQFYVADGKLSCQLYQRSADTFLGVPFNIAS
CCCCCCEEEEEECCCCCHHHHCCCHHHHHHHHHHCCCCEEEEEECCCCCCEECCCHHHHH
YALLTHMVAHVTGLEVGDFVHTLGDAHIYHNHLEQVELQLSRDPRPLPKLNIVRDVSSIE
HHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
SFRFEDFEILGYDPHPHIKGEVSV
HCCCCCEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MKQYHDLCRHILDQGVVKEDRTGTGTTSVFGYQMRFNLQEGFPLITTKKLHIRSIIHELL
CCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEECCCCCCEEEECHHHHHHHHHHHH
WFISGETNVRYLQDNGVRIWNEWADEEGNLGPVYGSQWRSFPRPDGSSVDQLAQVIEQIK
HHHCCCCCEEEEECCCEEEEECCCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHH
TNPDSRRLIVSAWNPGQLEEMALPPCHLLFQFYVADGKLSCQLYQRSADTFLGVPFNIAS
CCCCCCEEEEEECCCCCHHHHCCCHHHHHHHHHHCCCCEEEEEECCCCCCEECCCHHHHH
YALLTHMVAHVTGLEVGDFVHTLGDAHIYHNHLEQVELQLSRDPRPLPKLNIVRDVSSIE
HHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
SFRFEDFEILGYDPHPHIKGEVSV
HCCCCCEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA