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The map label for this gene is dfrA [H]

Identifier: 229915873

GI number: 229915873

Start: 113442

End: 113930

Strand: Reverse

Name: dfrA [H]

Synonym: EAT1b_0140

Alternate gene names: 229915873

Gene position: 113930-113442 (Counterclockwise)

Preceding gene: 229915874

Following gene: 229915866

Centisome position: 3.8

GC content: 48.26

Gene sequence:

>489_bases
ATGATTTCACACGTCGTCGCATATGGGCAGAATCGCGAAATCGGAAAAGACAATGATTTATTATGGCGCTTACCAGATGA
CTTGAAACATTTCAAGGCCATCACGTCTGGCGGGACGGTCGTGATGGGAAGAAAGACGTTTGAGTCCATCGGTAAAGCGT
TGCCGAACCGACGCAACATCGTCGTCACATCTGATGTTTCCTTTTCAGCCGATCAAGTCGAAGTGTGGCATGATTTAGAC
CGTCTCGCCGAAATGAGTCAACAGGAAGAATGGTTTGTCATCGGTGGGGCCATGTTATATGAACAGACGATGCCGATGAC
GAGCCGGATCTATGCAACGGAAGTCGCTGCATCGTTCGAGGCCGATACGTATTATCCCGAACAGACTGGGGAATGGTCCG
TTACGAGTACGATCGACCACCCGCAAGATGAAACACATGCATATGCCTTTTCATTCAAACAATACGACAAAACAGACATC
GCATCATGA

Upstream 100 bases:

>100_bases
ACATTGTCCGTGACGTATCTTCAATTGAATCATTCCGTTTTGAAGACTTCGAAATTCTCGGTTATGACCCGCATCCACAT
ATTAAAGGAGAAGTGAGCGT

Downstream 100 bases:

>100_bases
TGTCTGTTTTTTAGTCTGGCAATTTTTCTAGCCACTGTTCAATTTTTCTCGTGATGACTTCGTCCTGTCCTCCGTCCGCA
TAATCGTCATAGATCGCTTC

Product: dihydrofolate reductase region

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MISHVVAYGQNREIGKDNDLLWRLPDDLKHFKAITSGGTVVMGRKTFESIGKALPNRRNIVVTSDVSFSADQVEVWHDLD
RLAEMSQQEEWFVIGGAMLYEQTMPMTSRIYATEVAASFEADTYYPEQTGEWSVTSTIDHPQDETHAYAFSFKQYDKTDI
AS

Sequences:

>Translated_162_residues
MISHVVAYGQNREIGKDNDLLWRLPDDLKHFKAITSGGTVVMGRKTFESIGKALPNRRNIVVTSDVSFSADQVEVWHDLD
RLAEMSQQEEWFVIGGAMLYEQTMPMTSRIYATEVAASFEADTYYPEQTGEWSVTSTIDHPQDETHAYAFSFKQYDKTDI
AS
>Mature_162_residues
MISHVVAYGQNREIGKDNDLLWRLPDDLKHFKAITSGGTVVMGRKTFESIGKALPNRRNIVVTSDVSFSADQVEVWHDLD
RLAEMSQQEEWFVIGGAMLYEQTMPMTSRIYATEVAASFEADTYYPEQTGEWSVTSTIDHPQDETHAYAFSFKQYDKTDI
AS

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Escherichia coli, GI1786233, Length=159, Percent_Identity=34.5911949685535, Blast_Score=100, Evalue=9e-23,
Organism=Drosophila melanogaster, GI116008026, Length=140, Percent_Identity=32.1428571428571, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24647458, Length=140, Percent_Identity=32.1428571428571, Blast_Score=71, Evalue=3e-13,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 18409; Mature: 18409

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISHVVAYGQNREIGKDNDLLWRLPDDLKHFKAITSGGTVVMGRKTFESIGKALPNRRNI
CCCCEEEECCCCCCCCCCCEEEECCHHHHHHHHHCCCCEEEECHHHHHHHHHHCCCCCCE
VVTSDVSFSADQVEVWHDLDRLAEMSQQEEWFVIGGAMLYEQTMPMTSRIYATEVAASFE
EEECCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHCCCCHHHHHHHHHHHHCC
ADTYYPEQTGEWSVTSTIDHPQDETHAYAFSFKQYDKTDIAS
CCCCCCCCCCCEEEEECCCCCCCCCEEEEEEECCCCCCCCCC
>Mature Secondary Structure
MISHVVAYGQNREIGKDNDLLWRLPDDLKHFKAITSGGTVVMGRKTFESIGKALPNRRNI
CCCCEEEECCCCCCCCCCCEEEECCHHHHHHHHHCCCCEEEECHHHHHHHHHHCCCCCCE
VVTSDVSFSADQVEVWHDLDRLAEMSQQEEWFVIGGAMLYEQTMPMTSRIYATEVAASFE
EEECCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHCCCCHHHHHHHHHHHHCC
ADTYYPEQTGEWSVTSTIDHPQDETHAYAFSFKQYDKTDIAS
CCCCCCCCCCCEEEEECCCCCCCCCEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840350; 9384377 [H]