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The map label for this gene is alaS [H]

Identifier: 229587224

GI number: 229587224

Start: 1238526

End: 1241162

Strand: Direct

Name: alaS [H]

Synonym: RAF_ORF1214

Alternate gene names: 229587224

Gene position: 1238526-1241162 (Clockwise)

Preceding gene: 229587214

Following gene: 229587229

Centisome position: 96.87

GC content: 34.81

Gene sequence:

>2637_bases
ATGACTAAATTTACCACCGAAGAAGTTAGAAGTAAATTTATAACTTACTTTAAAGCAAATAATCATACACATGTACCGGC
CAGCTCGTTAATTCCACACAATGACCCTAGCTTAATGTTTGTTAATTCCGGAATGGTACAATTTAAAAATGTTTTTACCG
GACAAGGAAAAAGACCTTATAACAAAGCGGTAACTAGTCAAAAGTCTCTTAGAGCCGGCGGCAAGCATAACGACCTTGAG
AATGTCGGTTATACGGCAAGACATCATACCTTTTTTGAAATGCTAGGTAATTTTTCATTCGGTGACTATTTTAAAGAACA
AGCCATATATTACGCTTGGAATTTATTGACTAAAGAATTTGAACTCCCAAAAGACAAGCTTTATGCAACAATTTATCATA
CTGATGACGAAGCAGCTGCTTATTGGAAAAAAATAGCAGGTTTTGGGGATGATCGCATCATAAAGATCAAAACAAACGAT
AATTTTTGGTCAATGGGTGATACTGGCCCTTGCGGTCCTTGTTCCGAAATTTTTTACGACCACGGTGAACAAATATATGG
CGGACTTCCTGGTACTAAAGATGAAGACGGTGATAGATTTATCGAGATTTGGAATATGGTATTTATGCAATATGAACAAA
TCGATAAAGATACTAGCATAGAATTATCGCAAAAATCTATCGATACCGGTATGGGTCTTGAGCGTATGACAGCAGTATTA
CAGCATGTTAACAACAATTATGATATAGATTTATTTCAGGAAATAATTAATTTTACCGAAAATATAGTAAAAGTGAAAGT
AGAGGGAGAAGCTAAATTTTCTTACCGAGTTATTGCCGACCACTTACGAGCAAGTAGCTTTTTAATAGCTGACGGCGTTA
TTCCTTCAAATGAGGGGCGTGGTTACGTACTTCGCCGCATTATGCGAAGATCCATGAGGCATGCTCATATGCTTGGGTCA
AAAGAACCGTTAATGTATAAATTACTGCCAAAACTTGTTGATTTAATGGGAAATGTTTATCCTGAACTTAAAAGAGCAGA
AAGCTTTATAAGCAGTATCTTAGAACAAGAAGAAATTAGATTTAAAGCCACATTAGAACGTGGTCTCAAACTTCTTACCG
AAGAAACAGAAACACTAACAAAAGGTAATGAATTATCAGGAGAAGTAGCATTTAAACTATACGATACTTACGGATTTCCG
CTTGATTTAACCGAAGATATTCTAAAAAACCGTGATATTGCCGTTGATCACAAAGGGTTTGAAGAGCAAATGCTTATGCA
AAAAGAACGTGCTAGAAAATCTTGGCTTGGCTCCGGTGAATCAAAAACCGATCAGTTGTGGTTTGATATCAAAGAGCAAC
ATGGTAGCACGGAGTTTTTAGGATATACACTTAATGAAGCAAAATGTAAAATAATTGCATTAATCAAAAATAATAATTTA
GTTAATGATATAAAAGAAATAGATACACAATTCCTACTAATCAGCAACCAAACTCCTTTCTACGGTGAATCAGGCGGTCA
AATAGGTGACATCGGAACAATATTTGCAAAAGATTCTGAAGTAGAAGTAATAGATACTTTAAAATATTTAGGTTCTATTA
TAATCCACAAATGTATTTTGAAAAGGGGGCAGATTAACGTAGGCGAGAATGCTAATTTTAGTATAGACATAAGATATAGG
CAAAATTTAAGAATTCATCACTCGGCAACGCATATATTACATGCAGTACTGCATGAGGTACTTGGGAAACATGTTACTCA
AAAAGGTTCGTTAGTCGCTCCGACTTATTTACGCTTTGATATTAGCCATTCTAAAGCTGTGACTAATGAAGAAATCACTT
TAATCGAAGATAAGGTAAATGAGATTATTAGAGATAATCATGAAGTAACTACTACATTAATGGCAACTGAAGATGCAATT
AAACAAGGAGCTATGGCACTATTTGGCGAGAAATACGACTCTGAAGTAAGAGTAGTTAAGATGGGTGAGACTTCTTTAGA
ATTATGCGGCGGTACGCATGTCAGACGTACCGGTGACATCGGTTGCTTTAAAATCACAAGCGAAAGTGCTATAGCAGCAG
GTGTTCGTAGAATTGAAGCAGTTTGCGGTGAATTCGTAATTACATTAATGAGAGAAAAGGATAGTTTACTTAAATCGATA
GAAAGCAGTTTCAAAACTAATAAAAATGAACTTATTACTAAGGTTAATAATATCTTAGAGCGTAACAAGGAAGTTGAGAA
AGAGTTAGAAAAAACTCTTTTAGCTAGTTTAGATTTAAGTATAGAGCAAATTGAAAAGCAAGCTGCACAAATAACAGGAA
TAAAACTATTATATAAAAAAGTAGGAAATATAGACAATAAAATATTACGTCAAGCTGCCGAAAATTTAACAAAAAAAGTA
GAAGATTTGATAATGGTATATATTGCCGAGGGAATTGGTAAATTATCTATTACGGTTGCGGTGAGTAAAGCTATAACAGA
CAAATACAATGCAGATATAATTGCAAAAAAACTTTCTTTATTCTTAGGTGGCAGCGGCGGAGGTGGACAAGCAAGCCTTG
CTCAAGCCGGTGGAAATGATATAGGTAAGCTAACTAATATCCATGAAAAATTATACAGTTTATTGACAGTGTCGTAG

Upstream 100 bases:

>100_bases
GGCACGAGATTGCGTACGTACAATTACTACGTAATTTTCCTCGCCATGACGAAAAATGATCCTCTCCGGAATGACATAAC
ATTAAATAATATATCTTACC

Downstream 100 bases:

>100_bases
TGTACTAATTTAGGACGTCATTACGAGTGTGCTTTGTAGATGGATACCCGGTTGTCATTGTGAGCAACCGTAGTACTTGC
GTGGCAATCTCATCAAATAT

Product: alanyl-tRNA synthetase

Products: NA

Alternate protein names: Alanine--tRNA ligase; AlaRS [H]

Number of amino acids: Translated: 878; Mature: 877

Protein sequence:

>878_residues
MTKFTTEEVRSKFITYFKANNHTHVPASSLIPHNDPSLMFVNSGMVQFKNVFTGQGKRPYNKAVTSQKSLRAGGKHNDLE
NVGYTARHHTFFEMLGNFSFGDYFKEQAIYYAWNLLTKEFELPKDKLYATIYHTDDEAAAYWKKIAGFGDDRIIKIKTND
NFWSMGDTGPCGPCSEIFYDHGEQIYGGLPGTKDEDGDRFIEIWNMVFMQYEQIDKDTSIELSQKSIDTGMGLERMTAVL
QHVNNNYDIDLFQEIINFTENIVKVKVEGEAKFSYRVIADHLRASSFLIADGVIPSNEGRGYVLRRIMRRSMRHAHMLGS
KEPLMYKLLPKLVDLMGNVYPELKRAESFISSILEQEEIRFKATLERGLKLLTEETETLTKGNELSGEVAFKLYDTYGFP
LDLTEDILKNRDIAVDHKGFEEQMLMQKERARKSWLGSGESKTDQLWFDIKEQHGSTEFLGYTLNEAKCKIIALIKNNNL
VNDIKEIDTQFLLISNQTPFYGESGGQIGDIGTIFAKDSEVEVIDTLKYLGSIIIHKCILKRGQINVGENANFSIDIRYR
QNLRIHHSATHILHAVLHEVLGKHVTQKGSLVAPTYLRFDISHSKAVTNEEITLIEDKVNEIIRDNHEVTTTLMATEDAI
KQGAMALFGEKYDSEVRVVKMGETSLELCGGTHVRRTGDIGCFKITSESAIAAGVRRIEAVCGEFVITLMREKDSLLKSI
ESSFKTNKNELITKVNNILERNKEVEKELEKTLLASLDLSIEQIEKQAAQITGIKLLYKKVGNIDNKILRQAAENLTKKV
EDLIMVYIAEGIGKLSITVAVSKAITDKYNADIIAKKLSLFLGGSGGGGQASLAQAGGNDIGKLTNIHEKLYSLLTVS

Sequences:

>Translated_878_residues
MTKFTTEEVRSKFITYFKANNHTHVPASSLIPHNDPSLMFVNSGMVQFKNVFTGQGKRPYNKAVTSQKSLRAGGKHNDLE
NVGYTARHHTFFEMLGNFSFGDYFKEQAIYYAWNLLTKEFELPKDKLYATIYHTDDEAAAYWKKIAGFGDDRIIKIKTND
NFWSMGDTGPCGPCSEIFYDHGEQIYGGLPGTKDEDGDRFIEIWNMVFMQYEQIDKDTSIELSQKSIDTGMGLERMTAVL
QHVNNNYDIDLFQEIINFTENIVKVKVEGEAKFSYRVIADHLRASSFLIADGVIPSNEGRGYVLRRIMRRSMRHAHMLGS
KEPLMYKLLPKLVDLMGNVYPELKRAESFISSILEQEEIRFKATLERGLKLLTEETETLTKGNELSGEVAFKLYDTYGFP
LDLTEDILKNRDIAVDHKGFEEQMLMQKERARKSWLGSGESKTDQLWFDIKEQHGSTEFLGYTLNEAKCKIIALIKNNNL
VNDIKEIDTQFLLISNQTPFYGESGGQIGDIGTIFAKDSEVEVIDTLKYLGSIIIHKCILKRGQINVGENANFSIDIRYR
QNLRIHHSATHILHAVLHEVLGKHVTQKGSLVAPTYLRFDISHSKAVTNEEITLIEDKVNEIIRDNHEVTTTLMATEDAI
KQGAMALFGEKYDSEVRVVKMGETSLELCGGTHVRRTGDIGCFKITSESAIAAGVRRIEAVCGEFVITLMREKDSLLKSI
ESSFKTNKNELITKVNNILERNKEVEKELEKTLLASLDLSIEQIEKQAAQITGIKLLYKKVGNIDNKILRQAAENLTKKV
EDLIMVYIAEGIGKLSITVAVSKAITDKYNADIIAKKLSLFLGGSGGGGQASLAQAGGNDIGKLTNIHEKLYSLLTVS
>Mature_877_residues
TKFTTEEVRSKFITYFKANNHTHVPASSLIPHNDPSLMFVNSGMVQFKNVFTGQGKRPYNKAVTSQKSLRAGGKHNDLEN
VGYTARHHTFFEMLGNFSFGDYFKEQAIYYAWNLLTKEFELPKDKLYATIYHTDDEAAAYWKKIAGFGDDRIIKIKTNDN
FWSMGDTGPCGPCSEIFYDHGEQIYGGLPGTKDEDGDRFIEIWNMVFMQYEQIDKDTSIELSQKSIDTGMGLERMTAVLQ
HVNNNYDIDLFQEIINFTENIVKVKVEGEAKFSYRVIADHLRASSFLIADGVIPSNEGRGYVLRRIMRRSMRHAHMLGSK
EPLMYKLLPKLVDLMGNVYPELKRAESFISSILEQEEIRFKATLERGLKLLTEETETLTKGNELSGEVAFKLYDTYGFPL
DLTEDILKNRDIAVDHKGFEEQMLMQKERARKSWLGSGESKTDQLWFDIKEQHGSTEFLGYTLNEAKCKIIALIKNNNLV
NDIKEIDTQFLLISNQTPFYGESGGQIGDIGTIFAKDSEVEVIDTLKYLGSIIIHKCILKRGQINVGENANFSIDIRYRQ
NLRIHHSATHILHAVLHEVLGKHVTQKGSLVAPTYLRFDISHSKAVTNEEITLIEDKVNEIIRDNHEVTTTLMATEDAIK
QGAMALFGEKYDSEVRVVKMGETSLELCGGTHVRRTGDIGCFKITSESAIAAGVRRIEAVCGEFVITLMREKDSLLKSIE
SSFKTNKNELITKVNNILERNKEVEKELEKTLLASLDLSIEQIEKQAAQITGIKLLYKKVGNIDNKILRQAAENLTKKVE
DLIMVYIAEGIGKLSITVAVSKAITDKYNADIIAKKLSLFLGGSGGGGQASLAQAGGNDIGKLTNIHEKLYSLLTVS

Specific function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction:alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editin

COG id: COG0013

COG function: function code J; Alanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI109148542, Length=869, Percent_Identity=36.7088607594937, Blast_Score=488, Evalue=1e-138,
Organism=Homo sapiens, GI38569417, Length=954, Percent_Identity=29.4549266247379, Blast_Score=403, Evalue=1e-112,
Organism=Escherichia coli, GI1789048, Length=872, Percent_Identity=49.3119266055046, Blast_Score=832, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17506981, Length=966, Percent_Identity=32.712215320911, Blast_Score=476, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI17536681, Length=761, Percent_Identity=32.0630749014455, Blast_Score=335, Evalue=7e-92,
Organism=Saccharomyces cerevisiae, GI6324911, Length=781, Percent_Identity=39.4366197183099, Blast_Score=528, Evalue=1e-150,
Organism=Drosophila melanogaster, GI24582809, Length=967, Percent_Identity=33.0920372285419, Blast_Score=492, Evalue=1e-139,
Organism=Drosophila melanogaster, GI45552267, Length=967, Percent_Identity=33.0920372285419, Blast_Score=492, Evalue=1e-139,
Organism=Drosophila melanogaster, GI24658214, Length=994, Percent_Identity=27.4647887323944, Blast_Score=345, Evalue=9e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002318
- InterPro:   IPR018162
- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR023033
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: =6.1.1.7 [H]

Molecular weight: Translated: 98909; Mature: 98778

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: PS00018 EF_HAND_1 ; PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKFTTEEVRSKFITYFKANNHTHVPASSLIPHNDPSLMFVNSGMVQFKNVFTGQGKRPY
CCCCCHHHHHHHHHHHEECCCCCCCCHHHCCCCCCCCEEEEECCCHHHHHHHCCCCCCCH
NKAVTSQKSLRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKEQAIYYAWNLLTKEF
HHHHHCHHHHHCCCCCCCHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
ELPKDKLYATIYHTDDEAAAYWKKIAGFGDDRIIKIKTNDNFWSMGDTGPCGPCSEIFYD
CCCHHHEEEEEEECCCHHHHHHHHHHCCCCCEEEEEEECCCEEECCCCCCCCCHHHHHHC
HGEQIYGGLPGTKDEDGDRFIEIWNMVFMQYEQIDKDTSIELSQKSIDTGMGLERMTAVL
CCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHCCCCCHHHHHHHH
QHVNNNYDIDLFQEIINFTENIVKVKVEGEAKFSYRVIADHLRASSFLIADGVIPSNEGR
HHHCCCCCHHHHHHHHHHHCCEEEEEEECCCCEEHHHHHHHHHHCCEEEECCCCCCCCCC
GYVLRRIMRRSMRHAHMLGSKEPLMYKLLPKLVDLMGNVYPELKRAESFISSILEQEEIR
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FKATLERGLKLLTEETETLTKGNELSGEVAFKLYDTYGFPLDLTEDILKNRDIAVDHKGF
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCHHHHHCCCCEEECCCCH
EEQMLMQKERARKSWLGSGESKTDQLWFDIKEQHGSTEFLGYTLNEAKCKIIALIKNNNL
HHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHCCCCEEEEEEECCCCEEEEEEEECCCH
VNDIKEIDTQFLLISNQTPFYGESGGQIGDIGTIFAKDSEVEVIDTLKYLGSIIIHKCIL
HHHHHHCCCEEEEEECCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHH
KRGQINVGENANFSIDIRYRQNLRIHHSATHILHAVLHEVLGKHVTQKGSLVAPTYLRFD
HCCCCCCCCCCCEEEEEEEECCEEEEHHHHHHHHHHHHHHHHHHHCCCCCEECCEEEEEE
ISHSKAVTNEEITLIEDKVNEIIRDNHEVTTTLMATEDAIKQGAMALFGEKYDSEVRVVK
CCCCCCCCCCCEEEEHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCEEEEE
MGETSLELCGGTHVRRTGDIGCFKITSESAIAAGVRRIEAVCGEFVITLMREKDSLLKSI
ECCCHHHHCCCCCEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ESSFKTNKNELITKVNNILERNKEVEKELEKTLLASLDLSIEQIEKQAAQITGIKLLYKK
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
VGNIDNKILRQAAENLTKKVEDLIMVYIAEGIGKLSITVAVSKAITDKYNADIIAKKLSL
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHCCCCHHHHHHHHHH
FLGGSGGGGQASLAQAGGNDIGKLTNIHEKLYSLLTVS
HCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKFTTEEVRSKFITYFKANNHTHVPASSLIPHNDPSLMFVNSGMVQFKNVFTGQGKRPY
CCCCHHHHHHHHHHHEECCCCCCCCHHHCCCCCCCCEEEEECCCHHHHHHHCCCCCCCH
NKAVTSQKSLRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKEQAIYYAWNLLTKEF
HHHHHCHHHHHCCCCCCCHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
ELPKDKLYATIYHTDDEAAAYWKKIAGFGDDRIIKIKTNDNFWSMGDTGPCGPCSEIFYD
CCCHHHEEEEEEECCCHHHHHHHHHHCCCCCEEEEEEECCCEEECCCCCCCCCHHHHHHC
HGEQIYGGLPGTKDEDGDRFIEIWNMVFMQYEQIDKDTSIELSQKSIDTGMGLERMTAVL
CCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHCCCCCHHHHHHHH
QHVNNNYDIDLFQEIINFTENIVKVKVEGEAKFSYRVIADHLRASSFLIADGVIPSNEGR
HHHCCCCCHHHHHHHHHHHCCEEEEEEECCCCEEHHHHHHHHHHCCEEEECCCCCCCCCC
GYVLRRIMRRSMRHAHMLGSKEPLMYKLLPKLVDLMGNVYPELKRAESFISSILEQEEIR
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FKATLERGLKLLTEETETLTKGNELSGEVAFKLYDTYGFPLDLTEDILKNRDIAVDHKGF
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCHHHHHCCCCEEECCCCH
EEQMLMQKERARKSWLGSGESKTDQLWFDIKEQHGSTEFLGYTLNEAKCKIIALIKNNNL
HHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHCCCCEEEEEEECCCCEEEEEEEECCCH
VNDIKEIDTQFLLISNQTPFYGESGGQIGDIGTIFAKDSEVEVIDTLKYLGSIIIHKCIL
HHHHHHCCCEEEEEECCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHH
KRGQINVGENANFSIDIRYRQNLRIHHSATHILHAVLHEVLGKHVTQKGSLVAPTYLRFD
HCCCCCCCCCCCEEEEEEEECCEEEEHHHHHHHHHHHHHHHHHHHCCCCCEECCEEEEEE
ISHSKAVTNEEITLIEDKVNEIIRDNHEVTTTLMATEDAIKQGAMALFGEKYDSEVRVVK
CCCCCCCCCCCEEEEHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCEEEEE
MGETSLELCGGTHVRRTGDIGCFKITSESAIAAGVRRIEAVCGEFVITLMREKDSLLKSI
ECCCHHHHCCCCCEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ESSFKTNKNELITKVNNILERNKEVEKELEKTLLASLDLSIEQIEKQAAQITGIKLLYKK
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
VGNIDNKILRQAAENLTKKVEDLIMVYIAEGIGKLSITVAVSKAITDKYNADIIAKKLSL
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHCCCCHHHHHHHHHH
FLGGSGGGGQASLAQAGGNDIGKLTNIHEKLYSLLTVS
HCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA