Click here to switch to the map view.

The map label for this gene is greA [H]

Identifier: 229587229

GI number: 229587229

Start: 1246758

End: 1247315

Strand: Direct

Name: greA [H]

Synonym: RAF_ORF1219

Alternate gene names: 229587229

Gene position: 1246758-1247315 (Clockwise)

Preceding gene: 229587224

Following gene: 229587232

Centisome position: 97.51

GC content: 32.8

Gene sequence:

>558_bases
TTGCTGAAATCTATATTCTATATTATTGAAAATAGTATACTAAAATTACTAAATCTATACAATATAATTATGAATACAAA
ATTTCCTATCACCGCTAAAGGTTTTGAAAAATTAGAACACGAGCTTAAACATTTAAAACATGTAGAACGTAAAAAAATTA
GTGCAGATATAGCCGAAGCACGAGAACACGGCGATTTATCCGAGAATGCGGAATATGAAGCGGCACGTGAGAAACAAGCA
TTTATAGAAGGGCGTATTAAAGAGCTTGAAGATATGACGGCAAGAGCGGAAATTATTGATATTGGTAAATTATCGGGAGA
TAACATAAAATTCGGGGCTACGGTTACTTTGATCGACGACGATACTGAAGAAGAAGTAACATATATTATTGTAGGAGAAT
ATGAAGCTGATATAACTAGAAAAAGAGTTTCAATCGCTTCTCCTATAGCAAAAGCTTTGATTGGAAAATCGGTAGGTGAT
TTTGTAGAAGTTACAACACCGAAAGGATCTAAATCATACGAAGTAGTTACCATTGAATATAAAGAACTAGAACTTTAA

Upstream 100 bases:

>100_bases
CAGAATCCGCTTAGACAAGGACCACTTGCAATGACGATATTTAGGCAATGCCAAATAATTAAAAAGGCTTATTTGACAAA
TTTCACTATTACAAATATAC

Downstream 100 bases:

>100_bases
TTTGATTTTTTTGTCACTATAAACGTTAACCGTATGGCTTGTTATGTCATTCCCGCCTACGCAGGAACGACATCGAGTAG
GTTTTTCGATCCATGCAACA

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 185; Mature: 185

Protein sequence:

>185_residues
MLKSIFYIIENSILKLLNLYNIIMNTKFPITAKGFEKLEHELKHLKHVERKKISADIAEAREHGDLSENAEYEAAREKQA
FIEGRIKELEDMTARAEIIDIGKLSGDNIKFGATVTLIDDDTEEEVTYIIVGEYEADITRKRVSIASPIAKALIGKSVGD
FVEVTTPKGSKSYEVVTIEYKELEL

Sequences:

>Translated_185_residues
MLKSIFYIIENSILKLLNLYNIIMNTKFPITAKGFEKLEHELKHLKHVERKKISADIAEAREHGDLSENAEYEAAREKQA
FIEGRIKELEDMTARAEIIDIGKLSGDNIKFGATVTLIDDDTEEEVTYIIVGEYEADITRKRVSIASPIAKALIGKSVGD
FVEVTTPKGSKSYEVVTIEYKELEL
>Mature_185_residues
MLKSIFYIIENSILKLLNLYNIIMNTKFPITAKGFEKLEHELKHLKHVERKKISADIAEAREHGDLSENAEYEAAREKQA
FIEGRIKELEDMTARAEIIDIGKLSGDNIKFGATVTLIDDDTEEEVTYIIVGEYEADITRKRVSIASPIAKALIGKSVGD
FVEVTTPKGSKSYEVVTIEYKELEL

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082225, Length=153, Percent_Identity=52.2875816993464, Blast_Score=155, Evalue=2e-39,
Organism=Escherichia coli, GI87082261, Length=153, Percent_Identity=35.9477124183007, Blast_Score=86, Evalue=1e-18,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 20908; Mature: 20908

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00829 GREAB_1 ; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKSIFYIIENSILKLLNLYNIIMNTKFPITAKGFEKLEHELKHLKHVERKKISADIAEA
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
REHGDLSENAEYEAAREKQAFIEGRIKELEDMTARAEIIDIGKLSGDNIKFGATVTLIDD
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEECEEEEEECC
DTEEEVTYIIVGEYEADITRKRVSIASPIAKALIGKSVGDFVEVTTPKGSKSYEVVTIEY
CCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEEEEEE
KELEL
EEECC
>Mature Secondary Structure
MLKSIFYIIENSILKLLNLYNIIMNTKFPITAKGFEKLEHELKHLKHVERKKISADIAEA
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
REHGDLSENAEYEAAREKQAFIEGRIKELEDMTARAEIIDIGKLSGDNIKFGATVTLIDD
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEECEEEEEECC
DTEEEVTYIIVGEYEADITRKRVSIASPIAKALIGKSVGDFVEVTTPKGSKSYEVVTIEY
CCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEEEEEE
KELEL
EEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA