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The map label for this gene is tig

Identifier: 229587209

GI number: 229587209

Start: 1218945

End: 1220282

Strand: Direct

Name: tig

Synonym: RAF_ORF1194

Alternate gene names: 229587209

Gene position: 1218945-1220282 (Clockwise)

Preceding gene: 229587208

Following gene: 229587213

Centisome position: 95.34

GC content: 30.12

Gene sequence:

>1338_bases
ATGGGAATTACCATATTAAAAAACGAAGGATTGGATTTTCATGCAAGAATTTCTACTCCTTTAAGTGAGATAGATGACGA
TATTCAAAAAGAATTACTTGATTTAACCAAAAAAGTTAAAATAGCAGGTTTTAGAGTCGGTAAAGTACCGGTTTCAATTG
TTAAGAAAAAATACGGTACTTCCGTCAGGAATGATATAATAGAAAGAAGAATTAACCATTCAGTAAATCACGTTATTAAA
GAGCATAATCTAAATATAATCGGTAGACCTACAATTGAAGAATTACAAAACGAATCCGATAAAGCTTTAGAATTTACCGT
AAAAATGGAGCTATTACCTAAAATTACTATTCCGGATTTAAAAAAAATCTCACTAGATCGTCCAAAATTAGAAGTAAACT
CTAAAGATGTTGAAGAACAACTAGAAAAACTTGCTGCATTAACAAAAAATTATACTAAAGAAAGTAAAGCAAAAATAAAA
GACGGAGATCAGGTTACCATTGATGCAATCGGGTACATCAAGGAGAAAGCTTTTGAGGACGGTAAATTAAATGATTTTAA
GGTAATAATAGGTAGTAATGCACTTATTCCCGGTTTTGAGAAACAATTAATAGGTTCTAAAACAGGTAGTGAAGTAGATG
TTAATGTTACTTTTCCTGAAAATTACCATGCTAAAGATTTAGCAGGTAAGGATGCTCGTTTTGTAGTACAAATTAAAGCA
GTACATACTGCAGAACCTACCGTTATCGATGACGAATTTGCTAAAAAATTCCAAAGTAATAGCCTTGAAGAATTGCGTAC
ACATTTTACTAAACAAATAGAAAATGAGTCGGAAGAAGCTATTAATACTATAATGAAAATGAACTTATTTGATAAGTTAG
AAAAATTGTTAGATTTTGATGTACCGGAATCTTTATTAGAGCAAGAAAAAAATATTCTAAAGTCCGGAACCGATAAAAAT
GAACAAGATGAATCATTATTGAAAGATAAATCCTCTAAAGAAATAACAGCATATTATAATAAATTAGCCCTACGCCGTGT
GCGAATAGGCTTATTACTTGCTGAATATGCAAAATCTAAAAATTTGCAATTAGAGCCTGATGATTTAAGAAAAGTTATTA
TGCAACAAGCACGTAATTTTCCTGGGCAGGAAAATATGATATTTGATTTTTATAAAAATAATCCTAGAGCAATTGAAGGA
CTTAAAGGTCCTGCCTTAGAAGATAAAGCTGTACAATATATATTTAATCACGAAATAAAGCTTAAAGAAAAGAAATATAC
TAAGGAAGAGCTAGAGAAATATCTAGAAGCAGAAGAGCAACGTATTACTTTAATTTAA

Upstream 100 bases:

>100_bases
ATGCCTAATCCTAATGGGATTTTACAAAAACTATTGCATATTGCAGCTCTTTAGTTTATTTTGATTAGCAAACTATATAC
AGAATTAATGGATTATTAAA

Downstream 100 bases:

>100_bases
AGCTTATTCATTGTCATTGCCAGCGAACGTTAGTGAACGTGGCAATCTCAGGAATCCTCGTAATGACAATCCTCAGTATC
CACGCAGGCAATACCTAGTA

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MGITILKNEGLDFHARISTPLSEIDDDIQKELLDLTKKVKIAGFRVGKVPVSIVKKKYGTSVRNDIIERRINHSVNHVIK
EHNLNIIGRPTIEELQNESDKALEFTVKMELLPKITIPDLKKISLDRPKLEVNSKDVEEQLEKLAALTKNYTKESKAKIK
DGDQVTIDAIGYIKEKAFEDGKLNDFKVIIGSNALIPGFEKQLIGSKTGSEVDVNVTFPENYHAKDLAGKDARFVVQIKA
VHTAEPTVIDDEFAKKFQSNSLEELRTHFTKQIENESEEAINTIMKMNLFDKLEKLLDFDVPESLLEQEKNILKSGTDKN
EQDESLLKDKSSKEITAYYNKLALRRVRIGLLLAEYAKSKNLQLEPDDLRKVIMQQARNFPGQENMIFDFYKNNPRAIEG
LKGPALEDKAVQYIFNHEIKLKEKKYTKEELEKYLEAEEQRITLI

Sequences:

>Translated_445_residues
MGITILKNEGLDFHARISTPLSEIDDDIQKELLDLTKKVKIAGFRVGKVPVSIVKKKYGTSVRNDIIERRINHSVNHVIK
EHNLNIIGRPTIEELQNESDKALEFTVKMELLPKITIPDLKKISLDRPKLEVNSKDVEEQLEKLAALTKNYTKESKAKIK
DGDQVTIDAIGYIKEKAFEDGKLNDFKVIIGSNALIPGFEKQLIGSKTGSEVDVNVTFPENYHAKDLAGKDARFVVQIKA
VHTAEPTVIDDEFAKKFQSNSLEELRTHFTKQIENESEEAINTIMKMNLFDKLEKLLDFDVPESLLEQEKNILKSGTDKN
EQDESLLKDKSSKEITAYYNKLALRRVRIGLLLAEYAKSKNLQLEPDDLRKVIMQQARNFPGQENMIFDFYKNNPRAIEG
LKGPALEDKAVQYIFNHEIKLKEKKYTKEELEKYLEAEEQRITLI
>Mature_444_residues
GITILKNEGLDFHARISTPLSEIDDDIQKELLDLTKKVKIAGFRVGKVPVSIVKKKYGTSVRNDIIERRINHSVNHVIKE
HNLNIIGRPTIEELQNESDKALEFTVKMELLPKITIPDLKKISLDRPKLEVNSKDVEEQLEKLAALTKNYTKESKAKIKD
GDQVTIDAIGYIKEKAFEDGKLNDFKVIIGSNALIPGFEKQLIGSKTGSEVDVNVTFPENYHAKDLAGKDARFVVQIKAV
HTAEPTVIDDEFAKKFQSNSLEELRTHFTKQIENESEEAINTIMKMNLFDKLEKLLDFDVPESLLEQEKNILKSGTDKNE
QDESLLKDKSSKEITAYYNKLALRRVRIGLLLAEYAKSKNLQLEPDDLRKVIMQQARNFPGQENMIFDFYKNNPRAIEGL
KGPALEDKAVQYIFNHEIKLKEKKYTKEELEKYLEAEEQRITLI

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=434, Percent_Identity=31.1059907834101, Blast_Score=211, Evalue=9e-56,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_RICAE (C3PLX7)

Other databases:

- EMBL:   CP001612
- RefSeq:   YP_002845710.1
- GeneID:   7815466
- GenomeReviews:   CP001612_GR
- KEGG:   raf:RAF_ORF1194
- OMA:   YIKEKAF
- ProtClustDB:   PRK01490
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 50955; Mature: 50824

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGITILKNEGLDFHARISTPLSEIDDDIQKELLDLTKKVKIAGFRVGKVPVSIVKKKYGT
CCEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHCC
SVRNDIIERRINHSVNHVIKEHNLNIIGRPTIEELQNESDKALEFTVKMELLPKITIPDL
HHHHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHCCCCCCEEEEEEEEEECCCCCCCCC
KKISLDRPKLEVNSKDVEEQLEKLAALTKNYTKESKAKIKDGDQVTIDAIGYIKEKAFED
HHCCCCCCCEECCCHHHHHHHHHHHHHHHHHCHHHHCCCCCCCEEEEHHHHHHHHHHCCC
GKLNDFKVIIGSNALIPGFEKQLIGSKTGSEVDVNVTFPENYHAKDLAGKDARFVVQIKA
CCCCCEEEEECCCCCCCCHHHHHHCCCCCCEEEEEEECCCCCCCHHCCCCCCEEEEEEEE
VHTAEPTVIDDEFAKKFQSNSLEELRTHFTKQIENESEEAINTIMKMNLFDKLEKLLDFD
EECCCCCEECHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC
VPESLLEQEKNILKSGTDKNEQDESLLKDKSSKEITAYYNKLALRRVRIGLLLAEYAKSK
CCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
NLQLEPDDLRKVIMQQARNFPGQENMIFDFYKNNPRAIEGLKGPALEDKAVQYIFNHEIK
CCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEECCCCCCCCCHHHHHHHHCCCEE
LKEKKYTKEELEKYLEAEEQRITLI
EHHHHCCHHHHHHHHHHHHCEEECC
>Mature Secondary Structure 
GITILKNEGLDFHARISTPLSEIDDDIQKELLDLTKKVKIAGFRVGKVPVSIVKKKYGT
CEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHCC
SVRNDIIERRINHSVNHVIKEHNLNIIGRPTIEELQNESDKALEFTVKMELLPKITIPDL
HHHHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHCCCCCCEEEEEEEEEECCCCCCCCC
KKISLDRPKLEVNSKDVEEQLEKLAALTKNYTKESKAKIKDGDQVTIDAIGYIKEKAFED
HHCCCCCCCEECCCHHHHHHHHHHHHHHHHHCHHHHCCCCCCCEEEEHHHHHHHHHHCCC
GKLNDFKVIIGSNALIPGFEKQLIGSKTGSEVDVNVTFPENYHAKDLAGKDARFVVQIKA
CCCCCEEEEECCCCCCCCHHHHHHCCCCCCEEEEEEECCCCCCCHHCCCCCCEEEEEEEE
VHTAEPTVIDDEFAKKFQSNSLEELRTHFTKQIENESEEAINTIMKMNLFDKLEKLLDFD
EECCCCCEECHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC
VPESLLEQEKNILKSGTDKNEQDESLLKDKSSKEITAYYNKLALRRVRIGLLLAEYAKSK
CCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
NLQLEPDDLRKVIMQQARNFPGQENMIFDFYKNNPRAIEGLKGPALEDKAVQYIFNHEIK
CCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEECCCCCCCCCHHHHHHHHCCCEE
LKEKKYTKEELEKYLEAEEQRITLI
EHHHHCCHHHHHHHHHHHHCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA