Click here to switch to the map view.

The map label for this gene is addA [H]

Identifier: 229587088

GI number: 229587088

Start: 1044594

End: 1047062

Strand: Direct

Name: addA [H]

Synonym: RAF_ORF1022

Alternate gene names: 229587088

Gene position: 1044594-1047062 (Clockwise)

Preceding gene: 229587087

Following gene: 229587090

Centisome position: 81.7

GC content: 31.83

Gene sequence:

>2469_bases
ATGAGTGACTTACAAAGAGAAGCATCCGATCCTAATTACTCCGTTTGGGTATCGGCATCTGCCGGTACCGGTAAAACCAA
AATCCTAACCGATCGATTTTTACGTCTGTTAATAACGGGCGTAGAGCCTTCCAATATTTTATGCCTTACTTTTACTAATG
CTGCAGCTATAGAGATGCAAGTAAGGATTAATAGCAGACTTAAATATCTTTCTCTTTGCAACGCCGAGAAACTAGAAGAA
GCACTTTTCTTAATGAGCGGCAATAAGCCATCGCCGCAAGACACAGAAAACGCAAAAACTTTATATGATAAAATACTAAA
TAGCAATAAGCTTTTAAATATCTATACAATACATGCTTTTTGTCAAAAAATCCTTAACACTTTTCCCTTAGAAGCCGGCA
TAACACCAGAATTTAAAATCCTTGAAGAAACTAAATTACAAGATATCTTCCTAAATATCAGAAACGAAATTTACTTAAGC
GATGAACATAATGACTTAATGAAAATTTTACTTAACCGATTTCATGAAATAACTCTGCAAGATATTTTTACCGAGATAAT
CGAGCAGAAAATTAAATTCAGAAAGTTATTTATAAATAAGCAAATTCCCAAAGAAATATCTAATAAAAGATTAGCGTTAG
GGGAATTAAATAATATTTACGATAAGGTAAAAAACTTATTTGCCGAGTATGATGTGGAGATAGCACCGAAAGAAATTTTT
TTTACCAAAGATGGGAAAAAACGAAAAAGCCTTTTATCGAAAGAATTAATAAGGAAATATCCTAAATTATTACTAGAGCT
AGAAAAAATTACCTCTGAAATTTACCGATTAGATGAGCTGCGTCGCATAGAAGAGCTAGAATATCATACGAACTTACTTA
CTAAACTTGCGCATATTATTTTAAAAAAATACGATTCATATAAGGAAGAAAATAATTTACTCGATTACGACGATTTAATT
TACTACACCGAGAAATTACTAAATAATAAAACTACTCATAAGTGGTTGCTGCATAAGCTTGAAAGCGAGATAAATCATAT
TCTTGTCGATGAAGCACAAGACACAAGTCCCTCGCAGTGGAATATTATTACTACTTTAATAACAGAGTTTAATGCTGTAG
AGAGGCCAAATAATAGTGTTTTCATAGTCGGCGATGATAAACAATCGATCTTCAGTTTTCAAGGGGCTGATCTTCGTAAT
TTCAACTTAGTAAATGAGCGACTAAAAGCAAATCTTACAAATGCTAATAAGAAATTCAAAAATATCACCCTTGAATATTC
TTATAGATCATGTACAGAGATTTTACAATTTACTTATAATGTTTTAAAAAATATAAAATCCAATTACCCGGGTTTATTCC
TTTCGAATAATCCTTTAATATCCTCATTTCGTACACATCAAGGCTCTGTAACGGTTTGGCCATTAGTGACGAGTGAAAAA
CAAGAAGAGCTTTTTTGGGCACTACCTGAAGATTATGAAAATTCTAAATCTGCTGCCGATTTACTCATAGAAAAAATCGT
AAATTTTATAAAAGAACAAATAGAAAGCGAAGAAATTTTACCATCTACTGCAAACAGAATATCTGAAAAAGATTTCATGA
TATTAGTCAGAAAGAGAGATGAATTTAGTAATAATCTGATCAAAGAACTTAGCAAAGCTAAGCTTAAAGTTGAGGTAAGC
GATAGAATGAACCTTAAGGAAAATCTAACTATAATGGATTTAATTTCATTAGCTAAATTTGTGTTACTACCTGATGATGA
TTTAAATCTTGCCGGTTTACTAAAATCACCGATTATTGGAATAAGCGAGCAGCAATTATACGAACTTCTTGTAAATAAAA
GTGAGAATAGCTTATGGGATATACTCTCTTCACATGAGGAGATATATAATAAGTTAGATTCTCTAATTGAACTTTATAAA
ATATCTATTGTTGAGAACTTCTTTGATTTAGTGGTACATAATCTAAATTTACGAGAGCTTTACGATGATGATAGTGATGA
TATGATAACTGAGCTATTAACATTAAGCAAAAACTATGCAAATGATATAGATAATTCACTGCAAGGATTCGTTGCTTGGT
TTGAGAATAACGATATCTATATTAAACGTGATATAGAGCATTCAGACAAAATAAGAGTAATGACCGTCCACGGCTCTAAA
GGGCTTGAAACTCCTATTGTTATATTATGCGATTCTACTACTTTACCGATAAGCAGCAATAAATTTATTTGGGATGATAA
AGGAGAAATGTTTTTCTCTGCTAATGCTGCCGATACTCCTGAGTTTTTGCAAGAACTGAAAGAAGCTGAAAAATTAAAAG
ATTTGCAAGAATATATAAGACTACTCTATGTTGCAATGACAAGAGCTAAAGACAGGCTGATTATATGCGGTTTTAGTAAT
AAATCTACCACTCCTGAAAATTGCTGGTATGAAATAGCCAAACAAACTTGCGATAAATCTCTCAATTAG

Upstream 100 bases:

>100_bases
GATGTCATCCCGCGAGCTTGTAGCGGGATCCAGAAAAATAATTAAAAAGACTGGGCCCCGTGGTCAAGCCACGGGGTAAC
AGTAGAAAATCGATACATAA

Downstream 100 bases:

>100_bases
TAGCTGCTATATTAAATATAATAGACTATTAAGACGCACATATATTTAAATAGCATCTTATGTTAAAAATTTTAGAAAGT
TTCTTTGAAAATAAAGTAAG

Product: ATP-dependent helicase

Products: NA

Alternate protein names: ATP-dependent helicase/nuclease AddA [H]

Number of amino acids: Translated: 822; Mature: 821

Protein sequence:

>822_residues
MSDLQREASDPNYSVWVSASAGTGKTKILTDRFLRLLITGVEPSNILCLTFTNAAAIEMQVRINSRLKYLSLCNAEKLEE
ALFLMSGNKPSPQDTENAKTLYDKILNSNKLLNIYTIHAFCQKILNTFPLEAGITPEFKILEETKLQDIFLNIRNEIYLS
DEHNDLMKILLNRFHEITLQDIFTEIIEQKIKFRKLFINKQIPKEISNKRLALGELNNIYDKVKNLFAEYDVEIAPKEIF
FTKDGKKRKSLLSKELIRKYPKLLLELEKITSEIYRLDELRRIEELEYHTNLLTKLAHIILKKYDSYKEENNLLDYDDLI
YYTEKLLNNKTTHKWLLHKLESEINHILVDEAQDTSPSQWNIITTLITEFNAVERPNNSVFIVGDDKQSIFSFQGADLRN
FNLVNERLKANLTNANKKFKNITLEYSYRSCTEILQFTYNVLKNIKSNYPGLFLSNNPLISSFRTHQGSVTVWPLVTSEK
QEELFWALPEDYENSKSAADLLIEKIVNFIKEQIESEEILPSTANRISEKDFMILVRKRDEFSNNLIKELSKAKLKVEVS
DRMNLKENLTIMDLISLAKFVLLPDDDLNLAGLLKSPIIGISEQQLYELLVNKSENSLWDILSSHEEIYNKLDSLIELYK
ISIVENFFDLVVHNLNLRELYDDDSDDMITELLTLSKNYANDIDNSLQGFVAWFENNDIYIKRDIEHSDKIRVMTVHGSK
GLETPIVILCDSTTLPISSNKFIWDDKGEMFFSANAADTPEFLQELKEAEKLKDLQEYIRLLYVAMTRAKDRLIICGFSN
KSTTPENCWYEIAKQTCDKSLN

Sequences:

>Translated_822_residues
MSDLQREASDPNYSVWVSASAGTGKTKILTDRFLRLLITGVEPSNILCLTFTNAAAIEMQVRINSRLKYLSLCNAEKLEE
ALFLMSGNKPSPQDTENAKTLYDKILNSNKLLNIYTIHAFCQKILNTFPLEAGITPEFKILEETKLQDIFLNIRNEIYLS
DEHNDLMKILLNRFHEITLQDIFTEIIEQKIKFRKLFINKQIPKEISNKRLALGELNNIYDKVKNLFAEYDVEIAPKEIF
FTKDGKKRKSLLSKELIRKYPKLLLELEKITSEIYRLDELRRIEELEYHTNLLTKLAHIILKKYDSYKEENNLLDYDDLI
YYTEKLLNNKTTHKWLLHKLESEINHILVDEAQDTSPSQWNIITTLITEFNAVERPNNSVFIVGDDKQSIFSFQGADLRN
FNLVNERLKANLTNANKKFKNITLEYSYRSCTEILQFTYNVLKNIKSNYPGLFLSNNPLISSFRTHQGSVTVWPLVTSEK
QEELFWALPEDYENSKSAADLLIEKIVNFIKEQIESEEILPSTANRISEKDFMILVRKRDEFSNNLIKELSKAKLKVEVS
DRMNLKENLTIMDLISLAKFVLLPDDDLNLAGLLKSPIIGISEQQLYELLVNKSENSLWDILSSHEEIYNKLDSLIELYK
ISIVENFFDLVVHNLNLRELYDDDSDDMITELLTLSKNYANDIDNSLQGFVAWFENNDIYIKRDIEHSDKIRVMTVHGSK
GLETPIVILCDSTTLPISSNKFIWDDKGEMFFSANAADTPEFLQELKEAEKLKDLQEYIRLLYVAMTRAKDRLIICGFSN
KSTTPENCWYEIAKQTCDKSLN
>Mature_821_residues
SDLQREASDPNYSVWVSASAGTGKTKILTDRFLRLLITGVEPSNILCLTFTNAAAIEMQVRINSRLKYLSLCNAEKLEEA
LFLMSGNKPSPQDTENAKTLYDKILNSNKLLNIYTIHAFCQKILNTFPLEAGITPEFKILEETKLQDIFLNIRNEIYLSD
EHNDLMKILLNRFHEITLQDIFTEIIEQKIKFRKLFINKQIPKEISNKRLALGELNNIYDKVKNLFAEYDVEIAPKEIFF
TKDGKKRKSLLSKELIRKYPKLLLELEKITSEIYRLDELRRIEELEYHTNLLTKLAHIILKKYDSYKEENNLLDYDDLIY
YTEKLLNNKTTHKWLLHKLESEINHILVDEAQDTSPSQWNIITTLITEFNAVERPNNSVFIVGDDKQSIFSFQGADLRNF
NLVNERLKANLTNANKKFKNITLEYSYRSCTEILQFTYNVLKNIKSNYPGLFLSNNPLISSFRTHQGSVTVWPLVTSEKQ
EELFWALPEDYENSKSAADLLIEKIVNFIKEQIESEEILPSTANRISEKDFMILVRKRDEFSNNLIKELSKAKLKVEVSD
RMNLKENLTIMDLISLAKFVLLPDDDLNLAGLLKSPIIGISEQQLYELLVNKSENSLWDILSSHEEIYNKLDSLIELYKI
SIVENFFDLVVHNLNLRELYDDDSDDMITELLTLSKNYANDIDNSLQGFVAWFENNDIYIKRDIEHSDKIRVMTVHGSKG
LETPIVILCDSTTLPISSNKFIWDDKGEMFFSANAADTPEFLQELKEAEKLKDLQEYIRLLYVAMTRAKDRLIICGFSNK
STTPENCWYEIAKQTCDKSLN

Specific function: The heterodimer acts as both an ATP-dependent DNA helicase and an ATP-dependent, dual-direction single-stranded exonuclease. Recognizes the chi site generating a DNA molecule suitable for the initiation of homologous recombination. The AddA nuclease domai

COG id: COG1074

COG function: function code L; ATP-dependent exoDNAse (exonuclease V) beta subunit (contains helicase and exonuclease domains)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1789183, Length=512, Percent_Identity=23.4375, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI48994965, Length=462, Percent_Identity=23.1601731601732, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014152
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR011604
- InterPro:   IPR014016
- InterPro:   IPR011335 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 95471; Mature: 95340

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLQREASDPNYSVWVSASAGTGKTKILTDRFLRLLITGVEPSNILCLTFTNAAAIEMQ
CCCHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEE
VRINSRLKYLSLCNAEKLEEALFLMSGNKPSPQDTENAKTLYDKILNSNKLLNIYTIHAF
EEECCCHHHHHHCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEHHHH
CQKILNTFPLEAGITPEFKILEETKLQDIFLNIRNEIYLSDEHNDLMKILLNRFHEITLQ
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHH
DIFTEIIEQKIKFRKLFINKQIPKEISNKRLALGELNNIYDKVKNLFAEYDVEIAPKEIF
HHHHHHHHHHHHHHHHHHCCCCCHHHCCCCEEHHHHHHHHHHHHHHHHHCCCEECCCEEE
FTKDGKKRKSLLSKELIRKYPKLLLELEKITSEIYRLDELRRIEELEYHTNLLTKLAHII
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKKYDSYKEENNLLDYDDLIYYTEKLLNNKTTHKWLLHKLESEINHILVDEAQDTSPSQW
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEECCCCCCCCCH
NIITTLITEFNAVERPNNSVFIVGDDKQSIFSFQGADLRNFNLVNERLKANLTNANKKFK
HHHHHHHHHHHHCCCCCCEEEEEECCHHHHHEECCCCCCCHHHHHHHHHHCCCCCCHHHH
NITLEYSYRSCTEILQFTYNVLKNIKSNYPGLFLSNNPLISSFRTHQGSVTVWPLVTSEK
EEEEEECHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHCCCCCEEEEEEECCCC
QEELFWALPEDYENSKSAADLLIEKIVNFIKEQIESEEILPSTANRISEKDFMILVRKRD
CHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCEEEEEECCC
EFSNNLIKELSKAKLKVEVSDRMNLKENLTIMDLISLAKFVLLPDDDLNLAGLLKSPIIG
HHHHHHHHHHHHCEEEEEECCCCCCHHCCHHHHHHHHHHEEECCCCCCCHHHHHHCCCCC
ISEQQLYELLVNKSENSLWDILSSHEEIYNKLDSLIELYKISIVENFFDLVVHNLNLREL
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
YDDDSDDMITELLTLSKNYANDIDNSLQGFVAWFENNDIYIKRDIEHSDKIRVMTVHGSK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEEECCCCCCCEEEEEEECCC
GLETPIVILCDSTTLPISSNKFIWDDKGEMFFSANAADTPEFLQELKEAEKLKDLQEYIR
CCCCCEEEEECCCCCCCCCCCEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
LLYVAMTRAKDRLIICGFSNKSTTPENCWYEIAKQTCDKSLN
HHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SDLQREASDPNYSVWVSASAGTGKTKILTDRFLRLLITGVEPSNILCLTFTNAAAIEMQ
CCHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEE
VRINSRLKYLSLCNAEKLEEALFLMSGNKPSPQDTENAKTLYDKILNSNKLLNIYTIHAF
EEECCCHHHHHHCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEHHHH
CQKILNTFPLEAGITPEFKILEETKLQDIFLNIRNEIYLSDEHNDLMKILLNRFHEITLQ
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHH
DIFTEIIEQKIKFRKLFINKQIPKEISNKRLALGELNNIYDKVKNLFAEYDVEIAPKEIF
HHHHHHHHHHHHHHHHHHCCCCCHHHCCCCEEHHHHHHHHHHHHHHHHHCCCEECCCEEE
FTKDGKKRKSLLSKELIRKYPKLLLELEKITSEIYRLDELRRIEELEYHTNLLTKLAHII
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKKYDSYKEENNLLDYDDLIYYTEKLLNNKTTHKWLLHKLESEINHILVDEAQDTSPSQW
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEECCCCCCCCCH
NIITTLITEFNAVERPNNSVFIVGDDKQSIFSFQGADLRNFNLVNERLKANLTNANKKFK
HHHHHHHHHHHHCCCCCCEEEEEECCHHHHHEECCCCCCCHHHHHHHHHHCCCCCCHHHH
NITLEYSYRSCTEILQFTYNVLKNIKSNYPGLFLSNNPLISSFRTHQGSVTVWPLVTSEK
EEEEEECHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHCCCCCEEEEEEECCCC
QEELFWALPEDYENSKSAADLLIEKIVNFIKEQIESEEILPSTANRISEKDFMILVRKRD
CHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCEEEEEECCC
EFSNNLIKELSKAKLKVEVSDRMNLKENLTIMDLISLAKFVLLPDDDLNLAGLLKSPIIG
HHHHHHHHHHHHCEEEEEECCCCCCHHCCHHHHHHHHHHEEECCCCCCCHHHHHHCCCCC
ISEQQLYELLVNKSENSLWDILSSHEEIYNKLDSLIELYKISIVENFFDLVVHNLNLREL
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
YDDDSDDMITELLTLSKNYANDIDNSLQGFVAWFENNDIYIKRDIEHSDKIRVMTVHGSK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEEECCCCCCCEEEEEEECCC
GLETPIVILCDSTTLPISSNKFIWDDKGEMFFSANAADTPEFLQELKEAEKLKDLQEYIR
CCCCCEEEEECCCCCCCCCCCEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
LLYVAMTRAKDRLIICGFSNKSTTPENCWYEIAKQTCDKSLN
HHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA