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The map label for this gene is bcr2 [H]

Identifier: 229587057

GI number: 229587057

Start: 1005773

End: 1006945

Strand: Direct

Name: bcr2 [H]

Synonym: RAF_ORF0980

Alternate gene names: 229587057

Gene position: 1005773-1006945 (Clockwise)

Preceding gene: 229587056

Following gene: 229587058

Centisome position: 78.67

GC content: 31.8

Gene sequence:

>1173_bases
ATGAAATTAATTATTACCGTAATCATCATGGAAATACTCGCTGGAGCGGAAATAGATTTATTCATTCCAAGTTTTCCTGA
AATACAAGATACATTCAACCTTTCAACTTTTATGACAGAGTTACTACTTGGTGTTAATCTAACTGCTTATTGTATTACTT
CCCTTATAGTCGGTAATTTAGGAGATAGGTACGGACGCAGACCGATTATTATACTCGGTTTACTGATTTTTAATCTTGGT
AGTTTATTTTGTGTATTTGCAAATAATTATGCAGCTATATTTGGAAGATTTTTGCAAGGATGCGGGATATCATCGGTTGC
AGTGCTAGTATATATTGTTCTTGCCGATATTTATTCGGTACAAGAACAGCAAAGATTCATGGGCATTTTAAACGGCACTA
TTGTCCTTTCTATGGCAATAGCACCGGTTATAGGTAGCTACGTCAATTTATTTTGGGGCTGGCGTGGCAATTTTGTTTTG
TTGCTTATAATAGGTTTGATCAGCCTAATTCTTGGTATATTATTTATTCCAAAAAGAGAAGTTAATAAAAAAATACGTAT
TTCTTTAAAAGAATATATACCTATCTTTAAATCGCCAAAAGCTATATATTATATACTTACAATGATATTTCTTGCACAAG
GATATTGGATATTTATTGGGATTGCTCCTATTTTATATATGCAAGGTTTAGGTGTAACTTTAGAATATTTCGGACTTTAT
CAAGGAGCAATGGCAGTTCTATTCTCAATTATGAGCTTTTCAAGCGGTTATTGCTTTAAAAAATTCGGTATTAAAAATTG
CTTCTATTTTGGAGTTATATTTATAATACTATTCCTACTAGGCAATAGCATATTAGTTGTATATAATATTAATAATCCTT
TAACCATAACAATAGTTACTTTATTATTATCTCTTGGCGTGATATATCCGATTAACATATTATGGCCATTGCTTTTAGAA
GCTATTCCGGACGGTAAAGCTCGTATGATGGCTATTTTCACTGTGGCTAGATTGATTTTAACGGCATTTGGGCTGCAATT
AGTAGGATTTTTATATAACGGCACTTTTACTCCTCTTGGCATTACTATATCCAGTATTACACTATTTGGGTTAGTAACAT
TACCTAAATTATTTAAGTATGATAAAGTCTTTGAGATTTCTAGTAACGTGTAG

Upstream 100 bases:

>100_bases
ACTTGGTATCTATGCAACAATGCCTTGCAGGGATGACATCGCGCAAGGCTTTCGGCTTCAATAATACTTTGCTAAAATAA
CCTCAAACTAACAATTAATT

Downstream 100 bases:

>100_bases
ATGTCTTTATGTCATCAATTGTGTCGTCATTGCGAGGAAAAACTGTAAGTGTTGACGAAACAATCTCAGGAATTTGTTAT
TATTTCATAAGATTGCCACG

Product: MFS-type bicyclomycin resistance protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MKLIITVIIMEILAGAEIDLFIPSFPEIQDTFNLSTFMTELLLGVNLTAYCITSLIVGNLGDRYGRRPIIILGLLIFNLG
SLFCVFANNYAAIFGRFLQGCGISSVAVLVYIVLADIYSVQEQQRFMGILNGTIVLSMAIAPVIGSYVNLFWGWRGNFVL
LLIIGLISLILGILFIPKREVNKKIRISLKEYIPIFKSPKAIYYILTMIFLAQGYWIFIGIAPILYMQGLGVTLEYFGLY
QGAMAVLFSIMSFSSGYCFKKFGIKNCFYFGVIFIILFLLGNSILVVYNINNPLTITIVTLLLSLGVIYPINILWPLLLE
AIPDGKARMMAIFTVARLILTAFGLQLVGFLYNGTFTPLGITISSITLFGLVTLPKLFKYDKVFEISSNV

Sequences:

>Translated_390_residues
MKLIITVIIMEILAGAEIDLFIPSFPEIQDTFNLSTFMTELLLGVNLTAYCITSLIVGNLGDRYGRRPIIILGLLIFNLG
SLFCVFANNYAAIFGRFLQGCGISSVAVLVYIVLADIYSVQEQQRFMGILNGTIVLSMAIAPVIGSYVNLFWGWRGNFVL
LLIIGLISLILGILFIPKREVNKKIRISLKEYIPIFKSPKAIYYILTMIFLAQGYWIFIGIAPILYMQGLGVTLEYFGLY
QGAMAVLFSIMSFSSGYCFKKFGIKNCFYFGVIFIILFLLGNSILVVYNINNPLTITIVTLLLSLGVIYPINILWPLLLE
AIPDGKARMMAIFTVARLILTAFGLQLVGFLYNGTFTPLGITISSITLFGLVTLPKLFKYDKVFEISSNV
>Mature_390_residues
MKLIITVIIMEILAGAEIDLFIPSFPEIQDTFNLSTFMTELLLGVNLTAYCITSLIVGNLGDRYGRRPIIILGLLIFNLG
SLFCVFANNYAAIFGRFLQGCGISSVAVLVYIVLADIYSVQEQQRFMGILNGTIVLSMAIAPVIGSYVNLFWGWRGNFVL
LLIIGLISLILGILFIPKREVNKKIRISLKEYIPIFKSPKAIYYILTMIFLAQGYWIFIGIAPILYMQGLGVTLEYFGLY
QGAMAVLFSIMSFSSGYCFKKFGIKNCFYFGVIFIILFLLGNSILVVYNINNPLTITIVTLLLSLGVIYPINILWPLLLE
AIPDGKARMMAIFTVARLILTAFGLQLVGFLYNGTFTPLGITISSITLFGLVTLPKLFKYDKVFEISSNV

Specific function: Involved In Sulfonamide (Sulfathiazole) And Bicyclomycin Resistance. Probable Membrane Translocase. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=262, Percent_Identity=24.0458015267176, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI48994889, Length=159, Percent_Identity=32.0754716981132, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1790146, Length=258, Percent_Identity=24.8062015503876, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI87082312, Length=197, Percent_Identity=26.9035532994924, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1790794, Length=195, Percent_Identity=23.0769230769231, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87081738, Length=337, Percent_Identity=24.0356083086053, Blast_Score=65, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6324264, Length=183, Percent_Identity=29.5081967213115, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6322071, Length=164, Percent_Identity=30.4878048780488, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43452; Mature: 43452

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIITVIIMEILAGAEIDLFIPSFPEIQDTFNLSTFMTELLLGVNLTAYCITSLIVGNL
CHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC
GDRYGRRPIIILGLLIFNLGSLFCVFANNYAAIFGRFLQGCGISSVAVLVYIVLADIYSV
CHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
QEQQRFMGILNGTIVLSMAIAPVIGSYVNLFWGWRGNFVLLLIIGLISLILGILFIPKRE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHH
VNKKIRISLKEYIPIFKSPKAIYYILTMIFLAQGYWIFIGIAPILYMQGLGVTLEYFGLY
CCHHHEEEHHHHCCCCCCCHHHHHHHHHHHHHCCHHEEHHHHHHHHHCCCCCHHHHHHHH
QGAMAVLFSIMSFSSGYCFKKFGIKNCFYFGVIFIILFLLGNSILVVYNINNPLTITIVT
HHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHH
LLLSLGVIYPINILWPLLLEAIPDGKARMMAIFTVARLILTAFGLQLVGFLYNGTFTPLG
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
ITISSITLFGLVTLPKLFKYDKVFEISSNV
HHHHHHHHHHHHHHHHHHCCCHHEECCCCC
>Mature Secondary Structure
MKLIITVIIMEILAGAEIDLFIPSFPEIQDTFNLSTFMTELLLGVNLTAYCITSLIVGNL
CHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC
GDRYGRRPIIILGLLIFNLGSLFCVFANNYAAIFGRFLQGCGISSVAVLVYIVLADIYSV
CHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
QEQQRFMGILNGTIVLSMAIAPVIGSYVNLFWGWRGNFVLLLIIGLISLILGILFIPKRE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHH
VNKKIRISLKEYIPIFKSPKAIYYILTMIFLAQGYWIFIGIAPILYMQGLGVTLEYFGLY
CCHHHEEEHHHHCCCCCCCHHHHHHHHHHHHHCCHHEEHHHHHHHHHCCCCCHHHHHHHH
QGAMAVLFSIMSFSSGYCFKKFGIKNCFYFGVIFIILFLLGNSILVVYNINNPLTITIVT
HHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHH
LLLSLGVIYPINILWPLLLEAIPDGKARMMAIFTVARLILTAFGLQLVGFLYNGTFTPLG
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
ITISSITLFGLVTLPKLFKYDKVFEISSNV
HHHHHHHHHHHHHHHHHHCCCHHEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 9202461 [H]