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The map label for this gene is xth2 [H]

Identifier: 229587031

GI number: 229587031

Start: 964888

End: 965670

Strand: Direct

Name: xth2 [H]

Synonym: RAF_ORF0934

Alternate gene names: 229587031

Gene position: 964888-965670 (Clockwise)

Preceding gene: 229587030

Following gene: 229587032

Centisome position: 75.47

GC content: 30.27

Gene sequence:

>783_bases
ATGAAGATAGCTACTTGGAATATTAATTCCATAAAAACAAGACTTAATTTATTGCGTAATTTTTTATCTAAAGAAAATCC
GGATATTTTATTATTGCAAGAAATAAAATGCGAAACCGCAAAATTTCCTTTTGATGCATTATCCGATCTACCTTATAATT
CTTATGTTCATGGACAAAAATCATATAACGGCGTTGCTATAATTTCAAAATTTCCTGCCGATGAAATAATTAAGGATTTT
CCGAATAATTACTGCAGTGATCAAGCAAGATTCTTAGAAATAAAATTATCATTACCTATAGGGTTTTGTAATATAATCTC
GCTCTATGCTCCTAACGGTTCATTTGTCGGTAGCGATAAATTTGTAGCAAAACTAGCATTTTATGATAATTTTATCAATT
ATCTATCAACTAAAAAATCTTTTGACGAAAAAACTATCATAGGCGGTGATTTCAATATTGCACCGTTCGATATAGACGTA
TATTCACCTAAGCTACTTACTGAAACTACTTGTTTTACTGAGGTTGAACAAAAAAAATTACGCACTATTCTAAATTCCGG
ATTTGAGGATTTATACAGATTAATGCATCCGAGTAAACAAGAATTTTCATGGTGGGATTATAGAGCCGGATGTTTTGAAC
AAAATAAAGGTATGAGGATTGATATGGTTCTTGGTTGTAATAATACTATTGACTATTTAGAAAATTGCTATATGGATTAT
AATTTAAGGACTCAAGAAAAACCTTCAGATCATATACCGGTAATTGCGAGTTTTAAGGGATAG

Upstream 100 bases:

>100_bases
TAATTGCCAGAGCATTTATTACTAGTCTCTTAGTTTATCATTTTGGTTTTCTTGAGTGGCGATTCCGTAATACAAAATTA
GAAGATATAGCAAAACAAAC

Downstream 100 bases:

>100_bases
TCAATGTGGAATGTTGCTATCTTAGGATGCCGTATGTCATTTCTGCGTAGGCGGGAGTCCAAAAAAATAGCTTTAATATT
GATATAGGAGTTACATAATT

Product: Exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MKIATWNINSIKTRLNLLRNFLSKENPDILLLQEIKCETAKFPFDALSDLPYNSYVHGQKSYNGVAIISKFPADEIIKDF
PNNYCSDQARFLEIKLSLPIGFCNIISLYAPNGSFVGSDKFVAKLAFYDNFINYLSTKKSFDEKTIIGGDFNIAPFDIDV
YSPKLLTETTCFTEVEQKKLRTILNSGFEDLYRLMHPSKQEFSWWDYRAGCFEQNKGMRIDMVLGCNNTIDYLENCYMDY
NLRTQEKPSDHIPVIASFKG

Sequences:

>Translated_260_residues
MKIATWNINSIKTRLNLLRNFLSKENPDILLLQEIKCETAKFPFDALSDLPYNSYVHGQKSYNGVAIISKFPADEIIKDF
PNNYCSDQARFLEIKLSLPIGFCNIISLYAPNGSFVGSDKFVAKLAFYDNFINYLSTKKSFDEKTIIGGDFNIAPFDIDV
YSPKLLTETTCFTEVEQKKLRTILNSGFEDLYRLMHPSKQEFSWWDYRAGCFEQNKGMRIDMVLGCNNTIDYLENCYMDY
NLRTQEKPSDHIPVIASFKG
>Mature_260_residues
MKIATWNINSIKTRLNLLRNFLSKENPDILLLQEIKCETAKFPFDALSDLPYNSYVHGQKSYNGVAIISKFPADEIIKDF
PNNYCSDQARFLEIKLSLPIGFCNIISLYAPNGSFVGSDKFVAKLAFYDNFINYLSTKKSFDEKTIIGGDFNIAPFDIDV
YSPKLLTETTCFTEVEQKKLRTILNSGFEDLYRLMHPSKQEFSWWDYRAGCFEQNKGMRIDMVLGCNNTIDYLENCYMDY
NLRTQEKPSDHIPVIASFKG

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=269, Percent_Identity=23.0483271375465, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI18375503, Length=269, Percent_Identity=23.0483271375465, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI18375501, Length=269, Percent_Identity=23.0483271375465, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1788046, Length=267, Percent_Identity=34.0823970037453, Blast_Score=164, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI71989536, Length=265, Percent_Identity=25.6603773584906, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI221330655, Length=263, Percent_Identity=26.615969581749, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI17136678, Length=263, Percent_Identity=26.615969581749, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29966; Mature: 29966

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIATWNINSIKTRLNLLRNFLSKENPDILLLQEIKCETAKFPFDALSDLPYNSYVHGQK
CEEEEECHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHCCCCCCEECCCC
SYNGVAIISKFPADEIIKDFPNNYCSDQARFLEIKLSLPIGFCNIISLYAPNGSFVGSDK
CCCCEEEEECCCHHHHHHHCCCCCCCCCEEEEEEEEECCCCHHHEEEEECCCCCEECCHH
FVAKLAFYDNFINYLSTKKSFDEKTIIGGDFNIAPFDIDVYSPKLLTETTCFTEVEQKKL
HHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHH
RTILNSGFEDLYRLMHPSKQEFSWWDYRAGCFEQNKGMRIDMVLGCNNTIDYLENCYMDY
HHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCEEEEEEECCCHHHHHHHCEECC
NLRTQEKPSDHIPVIASFKG
CCCCCCCCCCCCCEEEECCC
>Mature Secondary Structure
MKIATWNINSIKTRLNLLRNFLSKENPDILLLQEIKCETAKFPFDALSDLPYNSYVHGQK
CEEEEECHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHCCCCCCEECCCC
SYNGVAIISKFPADEIIKDFPNNYCSDQARFLEIKLSLPIGFCNIISLYAPNGSFVGSDK
CCCCEEEEECCCHHHHHHHCCCCCCCCCEEEEEEEEECCCCHHHEEEEECCCCCEECCHH
FVAKLAFYDNFINYLSTKKSFDEKTIIGGDFNIAPFDIDVYSPKLLTETTCFTEVEQKKL
HHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHH
RTILNSGFEDLYRLMHPSKQEFSWWDYRAGCFEQNKGMRIDMVLGCNNTIDYLENCYMDY
HHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCEEEEEEECCCHHHHHHHCEECC
NLRTQEKPSDHIPVIASFKG
CCCCCCCCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]